FastQCFastQC Report
Wed 25 May 2016
SRR1033152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373545
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC27100.035.1878284
GTATCAA28850.034.9320681
ATCAACG27950.033.9546623
CAACGCA28400.033.4117165
AACGCAG30850.030.903386
CCGATCG650.006177918528.9015319
GGTATCA17600.028.8979761
TATCAAC36150.027.563312
ACGCAGA36100.025.6285537
CGCAGAG36100.025.36898
GTACATG59550.024.9897371
TACATGG58200.023.9041062
ACATGGG60200.023.1006953
GAGTACA43950.021.3231091
ACCGCGT1456.635935E-419.4337888
GCAGAGT46650.019.1282929
GTATAGG9750.018.3541951
CATGGGG37500.018.2888894
CCGGTCG1550.001038950218.1799959
CCTATAC10100.017.6763273
Produced by FastQC (version 0.11.5)