##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033152_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2373545
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.464068724207884	33.0	31.0	34.0	30.0	34.0
2	31.573470062712104	34.0	31.0	34.0	30.0	34.0
3	31.763870497504787	34.0	31.0	34.0	30.0	34.0
4	35.10921806833239	37.0	35.0	37.0	33.0	37.0
5	35.221070171410275	37.0	35.0	37.0	33.0	37.0
6	35.29440941713766	37.0	36.0	37.0	33.0	37.0
7	35.20869079794148	37.0	35.0	37.0	33.0	37.0
8	35.22431005099966	37.0	36.0	37.0	33.0	37.0
9	36.86288062792153	39.0	38.0	39.0	34.0	39.0
10-11	36.847694692959266	39.0	38.0	39.0	34.0	39.0
12-13	36.7969080004803	39.0	38.0	39.0	33.0	39.0
14-15	38.22819221881195	41.0	39.0	41.0	34.0	41.0
16-17	38.23752993939445	41.0	39.0	41.0	33.5	41.0
18-19	38.23245293432397	41.0	39.0	41.0	33.5	41.0
20-21	38.263593064382604	41.0	39.0	41.0	34.0	41.0
22-23	38.221486215765864	40.5	39.0	41.0	34.0	41.0
24-25	38.2178939097426	40.5	39.0	41.0	33.5	41.0
26-27	38.16975452329743	40.0	38.5	41.0	33.0	41.0
28-29	38.140628890541365	40.0	38.5	41.0	33.0	41.0
30-31	38.139589727601546	40.0	38.0	41.0	33.0	41.0
32-33	38.20182785664481	40.5	39.0	41.0	33.0	41.0
34-35	38.17361541491735	41.0	38.5	41.0	33.0	41.0
36-37	38.10002148684773	41.0	38.0	41.0	33.0	41.0
38-39	38.02305538761641	40.5	38.0	41.0	33.0	41.0
40-41	37.9675969909987	40.0	38.0	41.0	33.0	41.0
42-43	37.859574181235246	40.0	38.0	41.0	33.0	41.0
44-45	37.753880377241636	40.0	38.0	41.0	32.0	41.0
46-47	37.63688364871953	40.0	38.0	41.0	32.0	41.0
48-49	37.52400986709753	40.0	38.0	41.0	32.0	41.0
50-51	37.412104468211055	40.0	37.5	41.0	31.5	41.0
52-53	37.269727348754714	40.0	37.0	41.0	31.0	41.0
54-55	37.12622490831225	40.0	37.0	41.0	31.0	41.0
56-57	36.97961951427085	40.0	36.5	41.0	31.0	41.0
58-59	36.78755679795411	40.0	36.0	41.0	31.0	41.0
60-61	36.571212679768024	39.5	36.0	41.0	30.0	41.0
62-63	36.31685011238464	39.0	35.0	41.0	30.0	41.0
64-65	36.0550754251552	39.0	35.0	41.0	30.0	41.0
66-67	35.773329344925	38.5	35.0	40.5	29.5	41.0
68-69	35.473998807690606	38.0	35.0	40.0	29.0	41.0
70-71	35.129295210328856	37.0	35.0	40.0	29.0	41.0
72-73	34.76878487662968	37.0	34.0	39.5	29.0	41.0
74-75	34.39951949510121	36.0	34.0	39.0	28.5	41.0
76-77	34.06600907082023	36.0	34.0	39.0	28.0	40.0
78-79	33.68898714791588	35.0	34.0	37.5	28.0	39.5
80-81	33.31522638079329	35.0	34.0	37.0	27.5	39.0
82-83	33.07077009283582	35.0	34.0	37.0	27.5	39.0
84-85	32.78094474720302	35.0	34.0	36.0	27.0	38.0
86-87	32.50358514374069	35.0	34.0	36.0	27.0	37.0
88-89	32.116925316351704	35.0	33.0	36.0	26.0	37.0
90-91	31.90088412058756	35.0	33.0	35.0	26.0	36.5
92-93	31.699429545258255	35.0	33.0	35.0	25.0	36.0
94-95	31.509156135653633	35.0	33.0	35.0	25.0	36.0
96-97	31.316487574493003	35.0	33.0	35.0	24.5	36.0
98-99	31.10712225805704	35.0	33.0	35.0	23.5	35.5
100	30.938369822354325	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14917589344156212
1101	2	0.14879064938477882
1101	3	0.1659673108686981
1101	4	0.16386721272240834
1101	5	0.15686470808017816
1101	6	0.1945718503170326
1101	7	0.15885537418109408
1101	8	0.23823335127936218
1101	9	0.1825705421567818
1101	10-11	0.16819946518343443
1101	12-13	0.20552191879758652
1101	14-15	0.24571540552472726
1101	16-17	0.1326958691706821
1101	18-19	0.1754294067336417
1101	20-21	0.19211758545088742
1101	22-23	0.17820485402559427
1101	24-25	0.2131357962393352
1101	26-27	0.2472935740480935
1101	28-29	0.2762206963872984
1101	30-31	0.2159800254517492
1101	32-33	0.2587224921747264
1101	34-35	0.358547387703986
1101	36-37	0.2547126289349748
1101	38-39	0.2556753355904249
1101	40-41	0.2537628131776444
1101	42-43	0.24815374508580135
1101	44-45	0.20746489564719184
1101	46-47	0.261647372795359
1101	48-49	0.21755077425192582
1101	50-51	0.22128684839525192
1101	52-53	0.21084742623072117
1101	54-55	0.0986111085081589
1101	56-57	0.18138400491252327
1101	58-59	0.1819276317459071
1101	60-61	0.16979829163290816
1101	62-63	0.20856293059129172
1101	64-65	0.19029586060135983
1101	66-67	0.2832125072761329
1101	68-69	0.2129467912831231
1101	70-71	0.23392107282456465
1101	72-73	0.3181768647345038
1101	74-75	0.26931531886208404
1101	76-77	0.23681406504279323
1101	78-79	0.22053621786582767
1101	80-81	0.2639027522361488
1101	82-83	0.2871860700126021
1101	84-85	0.17063008425969883
1101	86-87	0.2611553156920934
1101	88-89	0.3594640767223254
1101	90-91	0.3968784432805208
1101	92-93	0.36475593499710257
1101	94-95	0.34179056603883495
1101	96-97	0.35554730653686306
1101	98-99	0.4087537951971534
1101	100	0.5659222100221619
1102	1	0.15524824893823563
1102	2	0.20284417296497992
1102	3	0.20023733314244296
1102	4	0.20485318078911519
1102	5	0.07765228034718064
1102	6	0.07413748601250347
1102	7	0.04317174897583698
1102	8	0.16743815581892818
1102	9	0.143857862946021
1102	10-11	0.15627530106220888
1102	12-13	0.13672417526284875
1102	14-15	0.19594309366426188
1102	16-17	0.22193069332026027
1102	18-19	0.22178442498549344
1102	20-21	0.2486506622243283
1102	22-23	0.21933522465299404
1102	24-25	0.22953832363464244
1102	26-27	0.2620427885086798
1102	28-29	0.2573776518065216
1102	30-31	0.2526334347716812
1102	32-33	0.2697710405259315
1102	34-35	0.26704238531188196
1102	36-37	0.2003678029803666
1102	38-39	0.19408799394692977
1102	40-41	0.22788491921188836
1102	42-43	0.2261137785227021
1102	44-45	0.28555250804548393
1102	46-47	0.23664207160911843
1102	48-49	0.27545646395815737
1102	50-51	0.2515111893395101
1102	52-53	0.17990567268593338
1102	54-55	0.29596791952481993
1102	56-57	0.23701868395855286
1102	58-59	0.2422648572890438
1102	60-61	0.21125698531547243
1102	62-63	0.2582081454163827
1102	64-65	0.2973949375815934
1102	66-67	0.19815777800464218
1102	68-69	0.18316284309981512
1102	70-71	0.22912310497516586
1102	72-73	0.30210914448992554
1102	74-75	0.2398950057678917
1102	76-77	0.18733436990042662
1102	78-79	0.2211543098944304
1102	80-81	0.22492032588759514
1102	82-83	0.21150348752001236
1102	84-85	0.20574176842465874
1102	86-87	0.24944499529014763
1102	88-89	0.39329129660501394
1102	90-91	0.36136763674665673
1102	92-93	0.39322860628675116
1102	94-95	0.42020807870472865
1102	96-97	0.4595083441323524
1102	98-99	0.3715016147009642
1102	100	0.3637882415979554
1103	1	0.1032256269052354
1103	2	0.11907245508421482
1103	3	0.1989253162683262
1103	4	0.14555656290342966
1103	5	0.09788994564214448
1103	6	0.0996109260637752
1103	7	0.18503912641721598
1103	8	0.09293406191256537
1103	9	0.05056613841869506
1103	10-11	0.17585339726434768
1103	12-13	0.16620971137043483
1103	14-15	0.17642769043694528
1103	16-17	0.16677596587150134
1103	18-19	0.1809289096144866
1103	20-21	0.14306348465442653
1103	22-23	0.2086469838034546
1103	24-25	0.14548679755452554
1103	26-27	0.25550457673782745
1103	28-29	0.21629014275652025
1103	30-31	0.23800045360415822
1103	32-33	0.20171639632869898
1103	34-35	0.25464761540654735
1103	36-37	0.3051140299569113
1103	38-39	0.21645874638132057
1103	40-41	0.20040958523085095
1103	42-43	0.12724148907190624
1103	44-45	0.11272878933050379
1103	46-47	0.15381087551702421
1103	48-49	0.1550294056871806
1103	50-51	0.15917571283051757
1103	52-53	0.16819518452002313
1103	54-55	0.20079561769650667
1103	56-57	0.06021847574496775
1103	58-59	0.10121651545252064
1103	60-61	0.1102239712542783
1103	62-63	0.23991688716541404
1103	64-65	0.2988317045739777
1103	66-67	0.1926157444632679
1103	68-69	0.12453651866302806
1103	70-71	0.15962919284876165
1103	72-73	0.19618383178805487
1103	74-75	0.15805151015342744
1103	76-77	0.094336229919179
1103	78-79	0.10187166334377196
1103	80-81	0.15235539054739888
1103	82-83	0.23075463922259587
1103	84-85	0.16380491580698475
1103	86-87	0.15036182176243074
1103	88-89	0.2526448425942718
1103	90-91	0.24015566582195902
1103	92-93	0.20477127735506784
1103	94-95	0.19495751874921652
1103	96-97	0.19210805521056784
1103	98-99	0.22147033179668796
1103	100	0.10730352444118907
1104	1	-0.05534554323450536
1104	2	0.0016595875698683926
1104	3	0.029717015253702073
1104	4	0.004133432700946571
1104	5	-0.044442486484427945
1104	6	-0.04312147335171801
1104	7	-0.0012427059649340322
1104	8	-0.004109302570633133
1104	9	0.03463609056635164
1104	10-11	0.029202276620765133
1104	12-13	0.05060181808785558
1104	14-15	-0.13619440821008055
1104	16-17	0.0073557945348312614
1104	18-19	0.017747088053418736
1104	20-21	-0.014349787618222365
1104	22-23	-0.025244172557982836
1104	24-25	-0.034455528517113976
1104	26-27	0.041657423386475045
1104	28-29	0.11237840154411316
1104	30-31	0.08277663465171514
1104	32-33	0.03610032527626572
1104	34-35	0.02425860963337101
1104	36-37	0.0997417720911784
1104	38-39	0.047424326230633085
1104	40-41	0.021763311774485317
1104	42-43	0.04395003583263701
1104	44-45	0.046401210999448494
1104	46-47	-0.038931477648645796
1104	48-49	-0.10403821290493198
1104	50-51	-0.07753739365644208
1104	52-53	-0.04718984388326675
1104	54-55	-0.14238490698590311
1104	56-57	-0.04534748788130116
1104	58-59	-0.12500909682702144
1104	60-61	-0.06575606011862334
1104	62-63	-0.12315337805040372
1104	64-65	-0.14598862817039304
1104	66-67	-0.10251323714005878
1104	68-69	-0.12585275144210328
1104	70-71	-0.0633870164823307
1104	72-73	-0.02945855681461751
1104	74-75	-0.0077770590094417
1104	76-77	-0.14702610393372595
1104	78-79	-0.07572678172350322
1104	80-81	-0.021379719096664473
1104	82-83	0.101603202619728
1104	84-85	0.03793924930635484
1104	86-87	0.1115345139586168
1104	88-89	0.20215694362855885
1104	90-91	0.16547994743792316
1104	92-93	0.14156793752082208
1104	94-95	0.16705918081898474
1104	96-97	0.27540652976738045
1104	98-99	0.2118374971421062
1104	100	0.31169647371671516
1105	1	0.2243751742326907
1105	2	0.21670794036470298
1105	3	0.17914681341589755
1105	4	0.18607877999840383
1105	5	0.17025997328845222
1105	6	0.27415231180938804
1105	7	0.1328859405580758
1105	8	0.15740026767132775
1105	9	0.24683784812022225
1105	10-11	0.2225087249976383
1105	12-13	0.2314733721988489
1105	14-15	0.3180108805022499
1105	16-17	0.3049493902974447
1105	18-19	0.2761905694546485
1105	20-21	0.3415392216510682
1105	22-23	0.3185165112027022
1105	24-25	0.3370644746930367
1105	26-27	0.4040871011682654
1105	28-29	0.3786510230279987
1105	30-31	0.3836485900307167
1105	32-33	0.3742813774009832
1105	34-35	0.37320126843518864
1105	36-37	0.3951231773317332
1105	38-39	0.4079138731990568
1105	40-41	0.3810358128779754
1105	42-43	0.379568189197272
1105	44-45	0.4023764928902267
1105	46-47	0.4147040104938924
1105	48-49	0.4416742384413155
1105	50-51	0.3715116011671995
1105	52-53	0.3584230929972776
1105	54-55	0.3462796730871247
1105	56-57	0.3855674031744343
1105	58-59	0.3656854980929296
1105	60-61	0.4082489958582656
1105	62-63	0.4264232842023077
1105	64-65	0.3406314914074713
1105	66-67	0.34463294479485995
1105	68-69	0.2964529345255542
1105	70-71	0.3555632664116146
1105	72-73	0.3882457889178994
1105	74-75	0.3600585013613298
1105	76-77	0.42677510789565076
1105	78-79	0.4414771828044053
1105	80-81	0.4256348469319846
1105	82-83	0.36409347187056085
1105	84-85	0.41127137883566434
1105	86-87	0.4393243297765963
1105	88-89	0.40149284507713645
1105	90-91	0.47576966293890166
1105	92-93	0.4767097857612619
1105	94-95	0.5418924539621166
1105	96-97	0.4701322630846647
1105	98-99	0.4175431269322445
1105	100	0.4443219702858734
1106	1	0.018899407927136735
1106	2	0.04242508853464955
1106	3	0.07822245185767329
1106	4	0.007145584924280968
1106	5	0.013652963390704542
1106	6	0.03728372692616233
1106	7	0.025391251972649798
1106	8	-0.023158746530128838
1106	9	-0.00673138348863489
1106	10-11	0.02217904695707773
1106	12-13	0.029460687176452893
1106	14-15	0.07663193982246952
1106	16-17	0.06591238386916842
1106	18-19	0.08517276598283985
1106	20-21	0.06534379278114244
1106	22-23	0.04235265844875613
1106	24-25	0.1607685869948554
1106	26-27	0.12208166695769052
1106	28-29	0.13058753554344094
1106	30-31	0.13694443484775576
1106	32-33	0.1864698361356787
1106	34-35	0.12020991873165343
1106	36-37	0.1853507592639616
1106	38-39	0.11648745139672201
1106	40-41	0.07966872298739958
1106	42-43	0.13398447935449553
1106	44-45	0.09671509020898839
1106	46-47	0.1401116737443573
1106	48-49	0.18848017786974935
1106	50-51	0.18027650723800548
1106	52-53	0.09758160899779256
1106	54-55	0.0776794695965961
1106	56-57	0.12825152896432712
1106	58-59	0.1566991693816675
1106	60-61	0.12762858365351093
1106	62-63	0.20865333764781724
1106	64-65	0.20716419997551583
1106	66-67	0.20182181284957323
1106	68-69	0.17039741513743678
1106	70-71	0.1750309944516033
1106	72-73	0.17620912943707623
1106	74-75	0.21005834542989277
1106	76-77	0.15802103905971876
1106	78-79	0.1771768276705501
1106	80-81	0.14498048065684088
1106	82-83	0.10326087596298095
1106	84-85	0.08817723133231681
1106	86-87	0.07290903758057254
1106	88-89	0.220511997355878
1106	90-91	0.29619496593317507
1106	92-93	0.22862244076685556
1106	94-95	0.21799182906688586
1106	96-97	0.18070312807532574
1106	98-99	0.25164349999820956
1106	100	0.32061228343518167
1107	1	-0.0239533160338965
1107	2	-0.008049510163179008
1107	3	0.0334325057955418
1107	4	-0.08620011930925386
1107	5	-0.05587571947454961
1107	6	-0.03897886501749781
1107	7	-0.07099163080368243
1107	8	-0.07093788317167338
1107	9	-0.07434687590164657
1107	10-11	-0.07808532378059851
1107	12-13	-0.14009348129007293
1107	14-15	-0.14797775681977754
1107	16-17	-0.23795591305232477
1107	18-19	-0.13663645255766
1107	20-21	-0.17534202226315188
1107	22-23	-0.26298593844346385
1107	24-25	-0.20763907595215159
1107	26-27	-0.1675113408721174
1107	28-29	-0.17276095455332552
1107	30-31	-0.09704336792277246
1107	32-33	-0.0979292845018449
1107	34-35	-0.1182127025984201
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2216	20-21	-0.22548399875243064
2216	22-23	-0.13337538068918775
2216	24-25	-0.12019406425564227
2216	26-27	-0.23518410345505458
2216	28-29	-0.2851033459376353
2216	30-31	-0.2920793402042605
2216	32-33	-0.2404111512352145
2216	34-35	-0.2623629769882143
2216	36-37	-0.3231192007697956
2216	38-39	-0.27178194297563607
2216	40-41	-0.24977395976278416
2216	42-43	-0.26434917246656653
2216	44-45	-0.225104560506324
2216	46-47	-0.1587880475052117
2216	48-49	-0.12101392988065385
2216	50-51	-0.15843787455692393
2216	52-53	-0.14621614290956586
2216	54-55	-0.08547345007444562
2216	56-57	-0.10641134894515858
2216	58-59	-0.20657632839424878
2216	60-61	-0.1267999711625336
2216	62-63	-0.047118952015978266
2216	64-65	0.0051219229401553434
2216	66-67	0.008706724079900141
2216	68-69	-0.06833614392549237
2216	70-71	-0.12423982733513839
2216	72-73	-0.05147182882789281
2216	74-75	-0.08334773458011568
2216	76-77	-0.16215626906389247
2216	78-79	-0.18308868908541598
2216	80-81	-0.11889226660920826
2216	82-83	-0.14943699842046954
2216	84-85	-0.21707135570425606
2216	86-87	-0.31459476217066396
2216	88-89	-0.1772056357340226
2216	90-91	-0.20162106966309778
2216	92-93	-0.2183706724177874
2216	94-95	-0.20168438792458332
2216	96-97	-0.20128414692329955
2216	98-99	-0.15497581967120766
2216	100	-0.15943689741665779
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27690.0
3	4943.0
4	3186.0
5	2522.0
6	2112.0
7	2255.0
8	2702.0
9	3029.0
10	3252.0
11	3639.0
12	4218.0
13	4555.0
14	5055.0
15	5198.0
16	5101.0
17	5189.0
18	5179.0
19	5173.0
20	5300.0
21	5552.0
22	6468.0
23	8836.0
24	13237.0
25	18095.0
26	18609.0
27	16359.0
28	17264.0
29	21715.0
30	26421.0
31	32554.0
32	41810.0
33	56181.0
34	77678.0
35	116480.0
36	211579.0
37	454780.0
38	769600.0
39	359088.0
40	941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.986241913721564	20.05714077986724	13.148297189542705	23.808320116868494
2	15.1906214515778	20.093960954490772	41.10960480383605	23.605812790095378
3	17.962790191774147	25.57975117295086	30.01548476418091	26.44197387109408
4	11.77560474362276	16.695024945029054	39.90627227234737	31.62309803900082
5	12.326492798386864	38.38637670261713	36.35229822754164	12.934832271454367
6	30.483753192289036	38.94480968880001	17.88587774728574	12.685559371625214
7	26.845568020224363	32.71446779375362	22.931353031021224	17.50861115500079
8	24.328016514682314	37.4651361622747	20.682204030826394	17.52464329221658
9	24.35281019895445	17.23357490141612	21.586857055689883	36.826757843939546
10-11	23.199213833098824	27.42351244167274	29.563116062861326	19.814157662367105
12-13	23.913431423879047	24.74707113653913	29.486430995123563	21.853066444458253
14-15	21.16242109678913	26.9681846156569	26.345513207194948	25.52388108035902
16-17	20.892261280317125	30.305485258382582	26.91155577365179	21.8906976876485
18-19	20.75184513215695	29.34738293479518	28.843162344449812	21.05760958859805
20-21	22.013126723727957	28.652132971448353	28.334384418015503	21.00035588680819
22-23	21.548773026299155	28.474592483595686	28.456178186347632	21.52045630375753
24-25	21.456625967317187	28.583092878042283	28.344231959742576	21.61604919489795
26-27	21.24703759515093	28.43202818962088	28.505751195075412	21.81518302015278
28-29	21.213463279131194	28.5653274293622	28.510230534677966	21.710978756828645
30-31	21.51560336721151	29.053503248862285	28.104644569894205	21.326248814032
32-33	21.086217348402336	28.652388661288974	28.693413537281963	21.567980453026728
34-35	21.191466679027084	28.38695540875223	28.770198479458358	21.651379432762333
36-37	21.611578614055958	28.838542345879052	28.109095896379166	21.440783143685824
38-39	21.425788875004713	28.94342693879649	28.299466691780378	21.331317494418414
40-41	21.16920401017058	28.605362413888923	28.649098697581504	21.57633487835899
42-43	21.138417601808754	28.500868183202876	28.679834849265617	21.680879365722753
44-45	21.30629317238956	28.679317786708484	28.58518859966612	21.42920044123583
46-47	21.33533669089544	28.962954879370876	28.38518481689798	21.316523612835702
48-49	21.193926782130724	28.6990132874814	28.49911331120228	21.607946619185594
50-51	21.189621073015513	28.596739004949047	28.50669886819927	21.706941053836164
52-53	21.250123243101125	28.601605826006864	28.536107911238002	21.61216301965401
54-55	21.3891746865565	29.00316161036523	28.290451043491522	21.317212659586747
56-57	21.20591821481146	28.707605606268586	28.360963578466443	21.725512600453513
58-59	21.06327991822998	28.31969516567614	28.77960940153007	21.83741551456381
60-61	21.395482289772016	28.757796111076704	28.330550475066136	21.516171124085144
62-63	21.254545803093812	28.711716822615564	28.371225295510115	21.66251207878051
64-65	21.15639392700329	28.49552167998987	28.560615683947333	21.787468709059503
66-67	21.069535276378947	28.534078784246518	28.370923622529105	22.025462316845427
68-69	21.566581559566544	28.659921296998498	28.311614169910847	21.46188297352411
70-71	21.490177358630476	28.440957979284565	28.355977848709244	21.71288681337571
72-73	21.205083286145715	28.57060700129429	28.5414517944777	21.682857918082295
74-75	21.218365026676842	28.620343665400533	28.449709616661128	21.711581691261493
76-77	21.239565936204958	28.643369471610413	28.291712239769858	21.82535235241477
78-79	21.345971056796024	28.68543857195101	28.355648003181834	21.612942368071135
80-81	21.29790434299004	28.43952761346209	28.494025650100273	21.768542393447596
82-83	21.134418482762573	28.524207730531053	28.628463137529504	21.71291064917687
84-85	21.127101478737423	28.40562002378367	28.528793721448803	21.938484776030105
86-87	21.30329489208306	28.567423020595818	28.388045967713392	21.741236119607734
88-89	21.12417154896661	28.458111540650844	28.440712850405596	21.977004059976952
90-91	21.07207560062294	28.359198756831955	28.569466592402126	21.999259050142978
92-93	21.256561213229176	28.534376444463	28.396682893043877	21.81237944926394
94-95	21.022957117062287	28.2889989898504	28.552036266214152	22.13600762687316
96-97	21.03429299529341	28.259527662637602	28.60961360014257	22.09656574192642
98-99	21.039792935612343	28.317178059057426	28.501336531544368	22.141692473785866
100	21.229779497459326	28.250473595212767	28.32497743271134	22.194769474616567
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	423.0
1	378.0
2	316.0
3	263.0
4	196.0
5	170.5
6	166.0
7	163.0
8	174.0
9	176.0
10	166.0
11	162.5
12	183.0
13	229.5
14	276.0
15	347.5
16	468.5
17	620.5
18	876.5
19	1293.0
20	1935.5
21	2843.0
22	4340.5
23	6692.0
24	9590.0
25	13080.0
26	17713.0
27	23574.0
28	30560.0
29	37942.0
30	45246.5
31	52669.5
32	59468.5
33	65888.5
34	72331.5
35	77693.0
36	81582.5
37	84666.0
38	87333.5
39	88568.5
40	88859.0
41	88392.0
42	86617.0
43	85111.5
44	83143.5
45	80676.5
46	78785.0
47	76103.0
48	73942.0
49	72143.5
50	69482.0
51	66543.0
52	61961.5
53	57961.0
54	55402.0
55	52321.0
56	48205.5
57	43869.5
58	39835.0
59	35682.5
60	31162.0
61	26484.0
62	22339.0
63	18271.0
64	14475.0
65	11451.0
66	8978.5
67	6746.5
68	5034.0
69	3722.0
70	2607.0
71	1861.5
72	1397.5
73	1057.0
74	772.0
75	518.5
76	336.0
77	225.5
78	146.0
79	97.0
80	71.0
81	42.5
82	25.0
83	20.0
84	16.0
85	15.0
86	12.5
87	10.5
88	11.5
89	7.5
90	5.5
91	7.0
92	6.0
93	2.0
94	3.0
95	4.5
96	2.0
97	1.0
98	2.0
99	2.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3290015567431837
2	0.11682946815838756
3	0.06471332963984251
4	0.03909763665740485
5	0.04301582653794219
6	0.010448506348099573
7	0.00716228257732632
8	0.016178332409960628
9	0.018495541479095615
10-11	0.021571109879947505
12-13	0.016747101908748306
14-15	0.01952775279171029
16-17	0.020412505345380014
18-19	0.020075456753505833
20-21	0.025257578853571345
22-23	0.016388987779881993
24-25	0.025952741574311843
26-27	0.019927997994560877
28-29	0.01908537651487543
30-31	0.02037037427139574
32-33	0.025046923483649983
34-35	0.015904480429062857
36-37	0.010048261145248984
38-39	0.014303499617660505
40-41	0.009669081479390532
42-43	0.008341952648885948
44-45	0.00888965661068149
46-47	0.008257690500917403
48-49	0.004550155990301427
50-51	0.006698840763499323
52-53	0.00787851083505895
54-55	0.0029913062528833453
56-57	0.002485733365072076
58-59	0.002485733365072076
60-61	0.0036232723626474323
62-63	0.0027174542719855745
64-65	0.00229614353214285
66-67	0.0015799152744102175
68-69	0.0027385198089777104
70-71	0.0020644226252293507
72-73	0.0017273740333551711
74-75	0.0018748327923001251
76-77	0.0029070441049148005
78-79	0.0029070441049148005
80-81	0.0027385198089777104
82-83	0.001980160477260806
84-85	0.0028438474939383915
86-87	0.002232946921166441
88-89	0.5970815805051094
90-91	0.6071509071873505
92-93	0.6033380449917739
94-95	0.5895611837989168
96-97	0.5890556109111055
98-99	0.5853059453265053
100	0.5870965159708369
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2373545.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.98954472186922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.57583736922626	61.09748611976165
2	11.622251057743274	17.19850128811375
3	3.012589720423604	6.687004255437771
4	1.1175090406403534	3.3073594055821034
5	0.534415203455174	1.9770568798046717
6	0.3042347631430805	1.3506114968113345
7	0.18408523511994768	0.9534267915580226
8	0.12840103696748123	0.7600267413631867
9	0.09235219702320012	0.6149787310629847
>10	0.41147744178620227	5.057833959837174
>50	0.014099685422719391	0.69255119698281
>100	0.0026902535950588955	0.27507746301564573
>500	5.699545366671099E-5	0.02808567066898881
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.426214796854494E-5	0.0
10-11	0.0	0.0	0.0	8.426214796854494E-5	0.0
12-13	0.0	0.0	0.0	8.426214796854494E-5	0.0
14-15	0.0	0.0	0.0	1.263932219528174E-4	0.0
16-17	0.0	0.0	0.0	1.263932219528174E-4	0.0
18-19	0.0	2.1065536992136236E-5	0.0	1.4745875894495365E-4	0.0
20-21	0.0	4.213107398427247E-5	0.0	1.8958983292922612E-4	0.0
22-23	0.0	4.213107398427247E-5	0.0	2.317209069134986E-4	0.0
24-25	0.0	4.213107398427247E-5	0.0	3.5811412886631604E-4	0.0
26-27	0.0	4.213107398427247E-5	0.0	5.898350357798146E-4	0.0
28-29	0.0	4.213107398427247E-5	0.0	0.0011586045345674928	0.0
30-31	0.0	4.213107398427247E-5	0.0	0.004465893842332882	0.0
32-33	0.0	4.213107398427247E-5	0.0	0.012028421622509791	0.0
34-35	0.0	4.213107398427247E-5	0.0	0.022687583340530726	0.0
36-37	0.0	4.213107398427247E-5	0.0	0.04103566606068139	0.0
38-39	0.0	4.213107398427247E-5	0.0	0.07356085517653974	0.0
40-41	0.0	4.213107398427247E-5	0.0	0.11524955288397734	0.0
42-43	0.0	4.213107398427247E-5	0.0	0.1586024280137937	0.0
44-45	0.0	4.213107398427247E-5	0.0	0.20433570882372148	0.0
46-47	0.0	6.31966109764087E-5	0.0	0.25942208805815775	0.0
48-49	0.0	8.426214796854494E-5	0.0	0.32331386175530696	0.0
50-51	0.0	1.263932219528174E-4	0.0	0.3947892287696252	0.0
52-53	0.0	1.6852429593708988E-4	0.0	0.4655904986001951	0.0
54-55	0.0	1.6852429593708988E-4	0.0	0.538266601223065	0.0
56-57	0.0	2.1065536992136236E-4	0.0	0.6259624317213281	0.0
58-59	0.0	2.1065536992136236E-4	0.0	0.7286990556319766	0.0
60-61	0.0	2.1065536992136236E-4	0.0	0.8379238649362031	0.0
62-63	0.0	2.1065536992136236E-4	0.0	0.9480544923310913	0.0
64-65	0.0	2.1065536992136236E-4	0.0	1.064020273472801	0.0
66-67	0.0	2.527864439056348E-4	0.0	1.2000193802940329	0.0
68-69	0.0	2.527864439056348E-4	0.0	1.3495846929382	0.0
70-71	0.0	2.527864439056348E-4	0.0	1.520152345963527	0.0
72-73	0.0	2.527864439056348E-4	0.0	1.7053816127353811	0.0
74-75	0.0	2.527864439056348E-4	0.0	1.886250313349863	0.0
76-77	0.0	2.527864439056348E-4	0.0	2.0759665395010414	0.0
78-79	0.0	2.527864439056348E-4	0.0	2.294984927608282	0.0
80-81	0.0	2.527864439056348E-4	0.0	2.535068852707659	0.0
82-83	0.0	2.527864439056348E-4	0.0	2.7893509497397355	0.0
84-85	0.0	2.738519808977711E-4	0.0	3.0373765822851473	0.0
86-87	0.0	2.949175178899073E-4	0.0	3.3004851393169288	0.0
88	0.0	2.949175178899073E-4	0.0	3.511709278737079	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	2710	0.0	35.187828	4
GTATCAA	2885	0.0	34.932068	1
ATCAACG	2795	0.0	33.954662	3
CAACGCA	2840	0.0	33.411716	5
AACGCAG	3085	0.0	30.90338	6
CCGATCG	65	0.0061779185	28.901531	9
GGTATCA	1760	0.0	28.897976	1
TATCAAC	3615	0.0	27.56331	2
ACGCAGA	3610	0.0	25.628553	7
CGCAGAG	3610	0.0	25.3689	8
GTACATG	5955	0.0	24.989737	1
TACATGG	5820	0.0	23.904106	2
ACATGGG	6020	0.0	23.100695	3
GAGTACA	4395	0.0	21.323109	1
ACCGCGT	145	6.635935E-4	19.433788	8
GCAGAGT	4665	0.0	19.128292	9
GTATAGG	975	0.0	18.354195	1
CATGGGG	3750	0.0	18.288889	4
CCGGTCG	155	0.0010389502	18.179995	9
CCTATAC	1010	0.0	17.676327	3
>>END_MODULE