FastQCFastQC Report
Wed 25 May 2016
SRR1033152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373545
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.18617721593650005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35280.14863842901651328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36000.046.7841641
GTATCAA65300.040.1291921
TCAACGC73600.035.1920284
ATCAACG74100.035.0814363
CAACGCA75350.034.3746955
TATCAAC79100.033.3400342
AACGCAG78500.033.106026
ACGCAGA89800.028.7307877
CGCAGAG89950.028.5771798
GTACATG57400.026.5553171
GCAGAGT101600.025.0691059
TACATGG58900.024.9011252
ACATGGG61250.023.4079953
ACCGTCC1703.4588647E-622.1143558
GAGTACT69850.021.59592812-13
AGAGTAC89600.020.90034910-11
CAGAGTA101150.020.00048410-11
AGTACTT74350.018.96153612-13
GTACTTT79400.018.82087714-15
CGTACAC2051.739798E-518.344533
Produced by FastQC (version 0.11.5)