FastQCFastQC Report
Wed 25 May 2016
SRR1033151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776051
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC40600.024.1813474
GTACATG61200.024.1787511
ATCAACG41800.023.6067583
CAACGCA42650.022.9089165
GTATCAA47100.022.7122731
TACATGG62250.022.2797812
AACGCAG44750.022.0354636
ACATGGG64850.020.7228433
GGTATCA33100.020.3593391
TATCAAC52250.019.6154582
ACGCAGA51400.018.3623757
CGCAGAG51500.018.053518
GACCGTC2102.161088E-517.8881977
GAGTACA48250.017.7758221
TATACTG17400.017.2778995
GTCTTAC10500.017.054931
TACACTG22450.016.948415
GTCCTAG10900.016.8614041
GTGTAGG11750.016.042711
CATGGGG39550.016.0342084
Produced by FastQC (version 0.11.5)