FastQCFastQC Report
Wed 25 May 2016
SRR1033151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776051
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44840.1615244100342537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36200.13040106251650277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48750.039.573661
GTATCAA79350.035.1645241
TCAACGC88350.030.7580034
ATCAACG89100.030.4990983
CAACGCA91050.029.8975435
AACGCAG93550.029.1383256
TATCAAC96250.028.4288392
CCGTCGT1057.4327916E-526.8531599
ACGCAGA106750.025.6233257
GTACATG65600.025.5354291
CGCAGAG107300.025.5330268
TACATGG65700.024.5451452
GCAGAGT120450.022.5113969
ACATGGG69450.022.0689773
GAGTACT76150.021.01264212-13
CGTATAC2751.4333636E-920.5156563
TAGACCG1405.201842E-420.1493055
AGAGTAC102400.020.14642710-11
GTACTTT86400.018.30226114-15
TATACCG1550.001031606918.1993735
Produced by FastQC (version 0.11.5)