FastQCFastQC Report
Wed 25 May 2016
SRR1033150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401615
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC29800.031.8429584
GTATCAA33300.031.1144281
ATCAACG30750.031.0190663
CAACGCA32050.029.6074925
AACGCAG32800.029.0664446
GGTATCA21350.027.132551
GTACATG63000.026.5381851
TATCAAC39050.024.918232
TACATGG62400.024.8597772
ACGCAGA39900.023.7765167
ACATGGG64300.023.3838923
CGCAGAG40000.023.1300188
TCTGTCG6050.020.1830928
GAGTACA45200.019.9011571
CTGTCGC6800.019.3383249
CCGGTCG2201.500397E-619.212759
CATGGGG40250.018.7904974
AGTACAT45550.018.369592
GCAGAGT48500.018.011139
GTATAGA9800.017.7811161
Produced by FastQC (version 0.11.5)