##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033150_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2401615
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55558655321523	33.0	31.0	34.0	30.0	34.0
2	31.710028043628974	34.0	31.0	34.0	30.0	34.0
3	31.828843923776294	34.0	31.0	34.0	30.0	34.0
4	35.15785794142691	37.0	35.0	37.0	33.0	37.0
5	35.2378145539564	37.0	35.0	37.0	33.0	37.0
6	35.34366790680438	37.0	36.0	37.0	33.0	37.0
7	35.27841806451076	37.0	36.0	37.0	33.0	37.0
8	35.273342313401606	37.0	36.0	37.0	33.0	37.0
9	36.91690591539443	39.0	38.0	39.0	34.0	39.0
10-11	36.91051646496212	39.0	38.0	39.0	34.0	39.0
12-13	36.85234206981552	39.0	38.0	39.0	34.0	39.0
14-15	38.30322616239489	41.0	39.0	41.0	34.0	41.0
16-17	38.314327442158714	41.0	39.0	41.0	34.0	41.0
18-19	38.31302415249738	41.0	39.0	41.0	34.0	41.0
20-21	38.34838390000063	41.0	39.0	41.0	34.0	41.0
22-23	38.31602067775226	41.0	39.0	41.0	34.0	41.0
24-25	38.310498976730244	41.0	39.0	41.0	34.0	41.0
26-27	38.268255528050915	40.5	39.0	41.0	33.5	41.0
28-29	38.23949737988812	40.0	38.5	41.0	33.5	41.0
30-31	38.235501318904156	40.0	39.0	41.0	33.0	41.0
32-33	38.30186728513938	41.0	39.0	41.0	34.0	41.0
34-35	38.27220037349866	41.0	39.0	41.0	33.0	41.0
36-37	38.1948534631904	41.0	39.0	41.0	33.0	41.0
38-39	38.11882899632123	41.0	38.0	41.0	33.0	41.0
40-41	38.061336434024604	41.0	38.0	41.0	33.0	41.0
42-43	37.955920911553264	40.5	38.0	41.0	33.0	41.0
44-45	37.84372495175121	40.0	38.0	41.0	33.0	41.0
46-47	37.729526381205986	40.0	38.0	41.0	32.0	41.0
48-49	37.616176614486506	40.0	38.0	41.0	32.0	41.0
50-51	37.50574176127314	40.0	38.0	41.0	32.0	41.0
52-53	37.364004430352075	40.0	37.0	41.0	31.5	41.0
54-55	37.221032721731	40.0	37.0	41.0	31.0	41.0
56-57	37.07248892932464	40.0	37.0	41.0	31.0	41.0
58-59	36.8820491627509	40.0	36.0	41.0	31.0	41.0
60-61	36.671739433672755	39.5	36.0	41.0	31.0	41.0
62-63	36.41543440559791	39.0	35.0	41.0	30.0	41.0
64-65	36.154014694278644	39.0	35.0	41.0	30.0	41.0
66-67	35.87088750694845	38.5	35.0	40.5	30.0	41.0
68-69	35.56989005315173	38.0	35.0	40.0	29.5	41.0
70-71	35.22360973761406	37.0	35.0	40.0	29.0	41.0
72-73	34.86020656932939	37.0	34.5	39.5	29.0	41.0
74-75	34.493959065045814	36.0	34.0	39.0	29.0	41.0
76-77	34.16332759413977	36.0	34.0	39.0	29.0	40.5
78-79	33.78819044684515	35.0	34.0	37.5	28.0	39.5
80-81	33.411599486179085	35.0	34.0	37.0	28.0	39.0
82-83	33.170881469344586	35.0	34.0	37.0	28.0	39.0
84-85	32.87967659262621	35.0	34.0	36.0	27.5	38.0
86-87	32.60727406349477	35.0	34.0	36.0	27.0	37.0
88-89	32.2236838544063	35.0	34.0	36.0	26.5	37.0
90-91	32.01394707311538	35.0	33.5	35.0	26.0	36.5
92-93	31.82152218403033	35.0	33.0	35.0	26.0	36.0
94-95	31.63963020717309	35.0	33.0	35.0	25.0	36.0
96-97	31.449911205584577	35.0	33.0	35.0	25.0	36.0
98-99	31.252770323303277	35.0	33.0	35.0	24.5	35.5
100	31.083621229880727	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13007267953790702
1101	2	0.15205280308461866
1101	3	0.19028376569183436
1101	4	0.2185129873092393
1101	5	0.1400043680692704
1101	6	0.12777666644732477
1101	7	0.15209946709315858
1101	8	0.1276992963811523
1101	9	0.2655047468043108
1101	10-11	0.2168640081860289
1101	12-13	0.19241579059573866
1101	14-15	0.20618114024907186
1101	16-17	0.1449624103792928
1101	18-19	0.15645442052812797
1101	20-21	0.17171360955990878
1101	22-23	0.1643234377778242
1101	24-25	0.14311115147584275
1101	26-27	0.14647903914490712
1101	28-29	0.22867637427093968
1101	30-31	0.18460337836850016
1101	32-33	0.2106360521536672
1101	34-35	0.20831746090561154
1101	36-37	0.18360846005603548
1101	38-39	0.13007671666759535
1101	40-41	0.15098178902358228
1101	42-43	0.1354044390617375
1101	44-45	0.055537084322260455
1101	46-47	0.13753642755492024
1101	48-49	0.09089600401282638
1101	50-51	0.053022261141464355
1101	52-53	0.03521795186902921
1101	54-55	0.013651379896771232
1101	56-57	0.15409637277758037
1101	58-59	0.16624759351906704
1101	60-61	0.1551797030840305
1101	62-63	0.20353670741947383
1101	64-65	0.24250966092389348
1101	66-67	0.1487051861846922
1101	68-69	0.15519661450350952
1101	70-71	0.1542067895063255
1101	72-73	0.13485846890781517
1101	74-75	0.07911001938032314
1101	76-77	0.04918199202155904
1101	78-79	0.04828132461354073
1101	80-81	0.1081462309973702
1101	82-83	0.05070408595107523
1101	84-85	0.01791985171578858
1101	86-87	0.06273888777568004
1101	88-89	0.2603276961194325
1101	90-91	0.2121260700933334
1101	92-93	0.22379977607804236
1101	94-95	0.23902999761961397
1101	96-97	0.20334937902424244
1101	98-99	0.25702049451547637
1101	100	0.40568112787175536
1102	1	0.030681991139587694
1102	2	0.04173255917658736
1102	3	-0.002788696082916431
1102	4	-0.07970813457600201
1102	5	-0.09224146555061452
1102	6	-0.11919030051964086
1102	7	-0.14284540143949442
1102	8	-0.0012757855710319177
1102	9	0.0019217745190474034
1102	10-11	-0.02566332831077034
1102	12-13	-0.046114113354015274
1102	14-15	-0.014742455101995233
1102	16-17	-0.031079585815369626
1102	18-19	-0.09115349272863682
1102	20-21	-0.08713218279733326
1102	22-23	0.010515354885775707
1102	24-25	-0.06977952546063904
1102	26-27	0.029115033918301947
1102	28-29	0.039969040983450554
1102	30-31	0.07075976376152227
1102	32-33	0.05668332079634553
1102	34-35	0.010398243978762878
1102	36-37	0.024969210119067498
1102	38-39	-0.043030359874599355
1102	40-41	-0.08885545279839846
1102	42-43	-0.02200953980261744
1102	44-45	-0.03081919562180957
1102	46-47	-0.04479651607573487
1102	48-49	-0.024787465716450185
1102	50-51	-0.06205739867102267
1102	52-53	-0.027506757573235063
1102	54-55	-0.11144959894465956
1102	56-57	-0.12168223025292946
1102	58-59	-0.12998260614624968
1102	60-61	-0.19043247397853236
1102	62-63	-0.0835524427994443
1102	64-65	-0.17143894416975058
1102	66-67	-0.18159190479941856
1102	68-69	-0.2203422067605061
1102	70-71	-0.21228703256001324
1102	72-73	-0.11976821884864108
1102	74-75	-0.16167924316467008
1102	76-77	-0.23533947601135452
1102	78-79	-0.20078293048125317
1102	80-81	-0.18846289721480503
1102	82-83	-0.1577050101679447
1102	84-85	-0.12327516008991779
1102	86-87	-0.13361578751028702
1102	88-89	0.12401176186456553
1102	90-91	0.1536628740728787
1102	92-93	0.12909262618005002
1102	94-95	0.12181591330629615
1102	96-97	0.03829264289880285
1102	98-99	0.004411778929661381
1102	100	-0.01715774153199945
1103	1	0.21727315977300776
1103	2	0.1752456497319308
1103	3	0.1944885107013299
1103	4	0.22292425133102967
1103	5	0.14625512963612408
1103	6	0.1926710326445118
1103	7	0.24821484895173995
1103	8	0.0870955082580096
1103	9	0.19949822420817043
1103	10-11	0.20867241588413066
1103	12-13	0.2538060532915054
1103	14-15	0.3105406625226692
1103	16-17	0.18671849395453677
1103	18-19	0.20247411375108726
1103	20-21	0.1992279449360339
1103	22-23	0.22147472975360216
1103	24-25	0.2930569906329268
1103	26-27	0.2892002071196771
1103	28-29	0.35498564209159866
1103	30-31	0.37422299812264015
1103	32-33	0.32286596470786577
1103	34-35	0.2519263918449397
1103	36-37	0.3394624004925433
1103	38-39	0.2949407097866086
1103	40-41	0.3546735515580792
1103	42-43	0.32027385274485454
1103	44-45	0.2583602126597171
1103	46-47	0.2718704149397908
1103	48-49	0.22981484786473771
1103	50-51	0.18148667357023385
1103	52-53	0.15191051180881487
1103	54-55	0.21982683594092833
1103	56-57	0.19069696231992594
1103	58-59	0.14217379321085133
1103	60-61	0.19159841254179355
1103	62-63	0.17444252399833715
1103	64-65	0.2385500060480723
1103	66-67	0.23847375945638305
1103	68-69	0.21410139176760623
1103	70-71	0.14319820619986956
1103	72-73	0.1725072432331629
1103	74-75	0.19246812603552144
1103	76-77	0.19389116307844034
1103	78-79	0.1276290202018231
1103	80-81	0.19152642309141044
1103	82-83	0.1479309074398003
1103	84-85	0.14651108742521046
1103	86-87	0.22970686612926983
1103	88-89	0.2539445115156198
1103	90-91	0.32134093946221753
1103	92-93	0.313271875852255
1103	94-95	0.2269437524130069
1103	96-97	0.1815691940978894
1103	98-99	0.23736154207308502
1103	100	0.2547842709029702
1104	1	0.06628059552431864
1104	2	0.06081377912486019
1104	3	0.1823368919328452
1104	4	0.10322595558352532
1104	5	-0.015171910446376558
1104	6	0.03563793812362803
1104	7	0.08958876683282568
1104	8	0.06537612645660573
1104	9	0.1397544048372268
1104	10-11	0.10931558123461116
1104	12-13	0.09778180568640948
1104	14-15	0.0546047823088216
1104	16-17	0.1475600364658689
1104	18-19	0.10695964931513657
1104	20-21	0.0742913794932818
1104	22-23	0.018824386460707387
1104	24-25	0.05813321386828818
1104	26-27	0.08574811208318067
1104	28-29	0.10310804704153043
1104	30-31	0.09918110417708448
1104	32-33	0.12130299827730084
1104	34-35	0.037242139897806226
1104	36-37	0.15820273265894969
1104	38-39	0.08592214754611405
1104	40-41	0.13053127496984018
1104	42-43	0.11768304295355136
1104	44-45	0.0560899678769502
1104	46-47	0.029529544088475745
1104	48-49	-0.014486557872182004
1104	50-51	-0.028098729018708468
1104	52-53	-0.011440257547207011
1104	54-55	-0.028458226068892145
1104	56-57	-0.036186908341420576
1104	58-59	-0.07039252473536095
1104	60-61	-0.11944920747503573
1104	62-63	-0.11575531040693932
1104	64-65	-0.22011797001641042
1104	66-67	-0.16613728833324615
1104	68-69	-0.15638084655636675
1104	70-71	-0.16132667914301635
1104	72-73	-0.18726591886079547
1104	74-75	-0.15332941935241706
1104	76-77	-0.1852344576982432
1104	78-79	-0.21888123109400226
1104	80-81	-0.1737694465386994
1104	82-83	-0.13828920708162684
1104	84-85	-0.1010538057876289
1104	86-87	-0.09035778962801544
1104	88-89	-0.02453794391057329
1104	90-91	-0.01134346174270462
1104	92-93	-0.05632024333686658
1104	94-95	-0.039082123458793916
1104	96-97	-0.018842960913097784
1104	98-99	0.06582449222098674
1104	100	0.09362182416085929
1105	1	0.1502247476775409
1105	2	0.1709972941176865
1105	3	0.10010007238054897
1105	4	0.15837945880670645
1105	5	0.1833834109497232
1105	6	0.10781620330132569
1105	7	0.09480560737785027
1105	8	0.09585647801139174
1105	9	0.05937640508861364
1105	10-11	0.13642517928747822
1105	12-13	0.17093486163057037
1105	14-15	0.1845246883475795
1105	16-17	0.22449064128831964
1105	18-19	0.14958354478897462
1105	20-21	0.2427493932152771
1105	22-23	0.32276546153012475
1105	24-25	0.2937835151286734
1105	26-27	0.35765878523811523
1105	28-29	0.3352632055235887
1105	30-31	0.31943741627990363
1105	32-33	0.29689956075842616
1105	34-35	0.23930827516216624
1105	36-37	0.2756164967731394
1105	38-39	0.2788236743347028
1105	40-41	0.2102483954723553
1105	42-43	0.17467863382709936
1105	44-45	0.2091497481878548
1105	46-47	0.1669406800302795
1105	48-49	0.17219248514658858
1105	50-51	0.23111134194193994
1105	52-53	0.15619408531992462
1105	54-55	0.13432122129664492
1105	56-57	0.19065938275233663
1105	58-59	0.15379714932229405
1105	60-61	0.16597606672823417
1105	62-63	0.16334863789151655
1105	64-65	0.14748311259472047
1105	66-67	0.263888071081567
1105	68-69	0.20503986833916343
1105	70-71	0.11508380057190237
1105	72-73	0.1812052765332126
1105	74-75	0.1446702721230011
1105	76-77	0.20516553054843456
1105	78-79	0.18463656130649753
1105	80-81	0.20171284757827124
1105	82-83	0.2154027629539499
1105	84-85	0.05222594112426293
1105	86-87	0.17938778369718023
1105	88-89	0.2611727155321546
1105	90-91	0.30783591183812575
1105	92-93	0.26653963263298763
1105	94-95	0.30048725621090355
1105	96-97	0.3538848942860291
1105	98-99	0.3571347504185134
1105	100	0.4015825746101811
1106	1	-0.01551010942144515
1106	2	-0.023591711002051596
1106	3	-0.05703600858265645
1106	4	-0.021558584193442698
1106	5	-0.012082110633976129
1106	6	0.01643131277187848
1106	7	-0.025431316064242537
1106	8	-0.10834013147032806
1106	9	-0.03252237837170924
1106	10-11	-0.07292688868113117
1106	12-13	-0.05677352449886541
1106	14-15	-0.04778652552471385
1106	16-17	-0.05623567116563777
1106	18-19	-0.06801329288941105
1106	20-21	-0.07093241853932142
1106	22-23	-0.06413301053928677
1106	24-25	0.002596387206544648
1106	26-27	0.045249406848796525
1106	28-29	0.0546479362448693
1106	30-31	-0.053009775541994486
1106	32-33	-0.0032267973661959104
1106	34-35	-0.06350665953778645
1106	36-37	-0.09367648134215756
1106	38-39	-0.06844738577706266
1106	40-41	-0.11862933208684012
1106	42-43	-0.07489550482450369
1106	44-45	-0.06482310244124534
1106	46-47	-0.09339622535505754
1106	48-49	-0.0777186215602299
1106	50-51	-0.03637901571108415
1106	52-53	-0.03350318395801821
1106	54-55	-0.07817041129654001
1106	56-57	-0.13066270865088825
1106	58-59	-0.2153555657910502
1106	60-61	-0.19969721722385714
1106	62-63	-0.14141231376611074
1106	64-65	-0.08029009768110029
1106	66-67	-0.11726870503932929
1106	68-69	-0.17649982068510184
1106	70-71	-0.164530121696302
1106	72-73	-0.010340205537126224
1106	74-75	0.04014484166913235
1106	76-77	-0.053796907090145396
1106	78-79	-0.11477105175092817
1106	80-81	-0.0658483270274175
1106	82-83	-0.13390444122174472
1106	84-85	-0.07655347307696303
1106	86-87	-0.07808397275516654
1106	88-89	-0.006688216893664389
1106	90-91	0.012238509347547222
1106	92-93	0.0026489147848813843
1106	94-95	-0.03404556537071812
1106	96-97	0.0017311854635622126
1106	98-99	-0.011668031810348367
1106	100	0.048588031980763446
1107	1	0.1483675514940117
1107	2	0.15020330339692123
1107	3	0.09531204056806786
1107	4	0.12004054326163782
1107	5	0.015442660090677407
1107	6	0.11784805579384283
1107	7	0.06993146109024906
1107	8	0.07606564152327877
1107	9	0.030851527995750416
1107	10-11	0.09102114858787758
1107	12-13	0.04642067843942499
1107	14-15	0.0037252712734670013
1107	16-17	0.01903667542115528
1107	18-19	0.06733951428166307
1107	20-21	0.024252018537445963
1107	22-23	0.015264997444319306
1107	24-25	0.10613683571108368
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2216	10-11	-0.1717257158030563
2216	12-13	-0.1961406186669663
2216	14-15	-0.2519651458878016
2216	16-17	-0.245703315273758
2216	18-19	-0.34426965033943446
2216	20-21	-0.2983023189325351
2216	22-23	-0.30064820737233333
2216	24-25	-0.2887876812643171
2216	26-27	-0.43779185900162076
2216	28-29	-0.38981425885108223
2216	30-31	-0.4874956271673696
2216	32-33	-0.3747862484468598
2216	34-35	-0.37297712020703244
2216	36-37	-0.36263707338846984
2216	38-39	-0.31564267876078844
2216	40-41	-0.3455906696174509
2216	42-43	-0.41807878091145056
2216	44-45	-0.36431799484021354
2216	46-47	-0.3441119936399346
2216	48-49	-0.32445415616096795
2216	50-51	-0.29911965888186387
2216	52-53	-0.30415781477856285
2216	54-55	-0.2805303471357945
2216	56-57	-0.3598078500506503
2216	58-59	-0.3630069672563039
2216	60-61	-0.25521082941827444
2216	62-63	-0.14790293674938226
2216	64-65	-0.18161229870733564
2216	66-67	-0.23834740272864963
2216	68-69	-0.26001961122598516
2216	70-71	-0.2243912187210242
2216	72-73	-0.18926864615064432
2216	74-75	-0.18031107947233238
2216	76-77	-0.1520402708910069
2216	78-79	-0.20565340412210276
2216	80-81	-0.13764221160138845
2216	82-83	-0.2214572113677491
2216	84-85	-0.26042539835799516
2216	86-87	-0.26911710158636026
2216	88-89	-0.2312346116631332
2216	90-91	-0.25020788070611744
2216	92-93	-0.26317117931151657
2216	94-95	-0.29318847160959294
2216	96-97	-0.2858472631926645
2216	98-99	-0.3055745697851471
2216	100	-0.22596324009064261
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26361.0
3	4759.0
4	3031.0
5	2465.0
6	2034.0
7	2251.0
8	2592.0
9	2958.0
10	3271.0
11	3618.0
12	4077.0
13	4548.0
14	5096.0
15	5167.0
16	5002.0
17	5164.0
18	5157.0
19	5000.0
20	5112.0
21	5410.0
22	6444.0
23	8414.0
24	13031.0
25	18295.0
26	19214.0
27	16832.0
28	17172.0
29	21286.0
30	26099.0
31	31565.0
32	41105.0
33	55118.0
34	76350.0
35	113797.0
36	208356.0
37	452646.0
38	789707.0
39	382003.0
40	1108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77201349560591	20.1946552320539	13.43448631974025	23.598844952599944
2	15.097773799781637	20.1408149491131	41.241379367846015	23.520031883259247
3	18.151174651004652	25.638449820669056	29.936017519766406	26.274358008559883
4	11.862378365769128	16.774401826179684	39.76489602772594	31.59832378032525
5	12.386989486996944	38.268380884222	36.14057772569714	13.204051903083917
6	30.472096391482545	38.707506619127216	18.040546786964462	12.779850202425777
7	26.851205251496076	32.636295836388776	23.00061337967202	17.511885532443124
8	24.29690024754042	37.465309358893634	20.637599824089715	17.600190569476233
9	24.21752728878005	17.50942248986952	21.70391933932208	36.56913088202835
10-11	23.272369934348443	27.486817941301965	29.32671493788955	19.91409718646004
12-13	23.987803669310605	24.747981645950755	29.42436617477659	21.839848509962046
14-15	21.236744422172794	26.93761637609171	26.384154485396426	25.44148471633907
16-17	20.93352654779632	30.26645979197724	26.91289502469981	21.887118635526626
18-19	20.761972983609834	29.36134369787621	28.823806241631562	21.05287707688239
20-21	22.024142961919278	28.632616571797698	28.311254623534637	21.031985842748384
22-23	21.54324981254309	28.473293871424026	28.43181493062793	21.551641385404952
24-25	21.488885482906316	28.542393098886965	28.352118530414778	21.61660288779194
26-27	21.220228470595536	28.459996405874627	28.466160143481815	21.85361498004802
28-29	21.242299478246963	28.499322820531614	28.526289223101063	21.732088478120357
30-31	21.486004364207005	29.068475653168196	28.087663962307648	21.357856020317158
32-33	21.12239296560939	28.639206765169906	28.67490016676357	21.56350010245714
34-35	21.214428735867692	28.286848939462843	28.802041948396578	21.696680376272887
36-37	21.66490933940319	28.774693154835525	28.14503280205416	21.415364703707127
38-39	21.415808876733323	28.92502011949889	28.302809650279904	21.35636135348788
40-41	21.113722815251883	28.653378832370446	28.643530285530822	21.589368066846855
42-43	21.087938922142722	28.500737488308875	28.638969698433492	21.77235389111491
44-45	21.32292850228691	28.659945960340792	28.520151604209985	21.49697393316231
46-47	21.31585226555821	28.917197637894628	28.381722124895425	21.385227971651737
48-49	21.23549350200499	28.7005675595771	28.462800488026286	21.60113845039163
50-51	21.270903260718164	28.55073571191066	28.468951772668966	21.709409254702212
52-53	21.214745276102796	28.533228173921948	28.56987295296747	21.682153597007787
54-55	21.439913855258265	29.018281814644315	28.261933647145927	21.2798706829515
56-57	21.233464804309225	28.652233729633934	28.383760614004988	21.730540852051845
58-59	21.093386138350002	28.22822610246469	28.79623674730314	21.88215101188217
60-61	21.43950941203439	28.714528030400665	28.318134319983564	21.52782823758139
62-63	21.192645728183923	28.787158922409468	28.32697561526069	21.69321973414592
64-65	21.177360941805475	28.492467908846653	28.531567534818553	21.79860361452932
66-67	21.063811758259124	28.542477290390895	28.345668659042634	22.04804229230735
68-69	21.58636561614893	28.63842707988937	28.28590416340141	21.48930314056029
70-71	21.514743005855564	28.42578529603123	28.3755888093689	21.683882888744307
72-73	21.20628664141789	28.56422792021131	28.52223453130735	21.707250907063447
74-75	21.22285539875235	28.605349981959638	28.421365366962593	21.750429252325414
76-77	21.25547047090813	28.637160476862668	28.29718970822767	21.810179344001533
78-79	21.45889130956188	28.7199733837011	28.31893837943716	21.50219692729986
80-81	21.29543303453758	28.456953882297487	28.485435736982563	21.76217734618237
82-83	21.150755216813057	28.516877765387694	28.62813868640493	21.70422833139432
84-85	21.18151464609546	28.46449441182539	28.45685349573993	21.897137446339222
86-87	21.327021322399325	28.612913528253937	28.323080965322944	21.736984184023793
88-89	21.130774671294102	28.48931735944536	28.43249714275781	21.947410826502725
90-91	21.112394391214153	28.356838723947597	28.49591891792258	22.03484796691567
92-93	21.250261028711797	28.600410650264674	28.337109391608834	21.812218929414694
94-95	21.019791643760882	28.322207667233158	28.55138068207401	22.106620006931944
96-97	21.073844674377774	28.24912295103939	28.601680717320505	22.075351657262335
98-99	21.075032291678884	28.363549018852307	28.476548953515064	22.084869735953745
100	21.253461687309745	28.274467197528523	28.273713291036383	22.198357824125342
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	335.0
1	327.5
2	321.5
3	275.0
4	202.0
5	178.0
6	162.0
7	157.5
8	161.5
9	159.5
10	170.0
11	178.5
12	200.5
13	243.5
14	283.0
15	347.0
16	431.0
17	575.0
18	848.0
19	1263.0
20	1888.5
21	2884.5
22	4394.0
23	6680.0
24	9649.5
25	13217.5
26	18038.0
27	23994.0
28	31198.5
29	38890.0
30	46256.5
31	53674.5
32	60935.5
33	67610.5
34	73356.5
35	78225.5
36	81839.5
37	85110.0
38	87784.5
39	88800.0
40	89537.5
41	89353.5
42	87362.5
43	85348.5
44	83412.0
45	81286.5
46	79204.0
47	76887.0
48	74778.0
49	73049.5
50	70449.5
51	67139.5
52	62462.0
53	58278.0
54	55864.5
55	52901.5
56	48826.5
57	44365.5
58	40569.5
59	36525.5
60	31975.5
61	27169.5
62	22798.5
63	18939.0
64	15402.0
65	12208.5
66	9284.0
67	6825.5
68	5051.5
69	3804.0
70	2661.0
71	1900.5
72	1478.0
73	1045.0
74	741.0
75	548.5
76	344.5
77	236.5
78	176.5
79	104.5
80	67.5
81	43.0
82	30.0
83	21.5
84	14.0
85	11.5
86	9.5
87	6.0
88	5.0
89	5.0
90	2.5
91	2.0
92	3.0
93	5.0
94	4.0
95	2.0
96	1.5
97	0.5
98	0.5
99	1.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33090233030689764
2	0.11925308594425
3	0.06591397871848735
4	0.040597681143730364
5	0.04430352075582473
6	0.010034914005783609
7	0.006912015456265887
8	0.01628071110481905
9	0.01852919806047181
10-11	0.022047663759595104
12-13	0.01669709757808808
14-15	0.018987223181067738
16-17	0.02013228598255757
18-19	0.01998655071691341
20-21	0.024316970038911315
22-23	0.016509723665117017
24-25	0.025441213516737695
26-27	0.019757538156615444
28-29	0.019944912069586505
30-31	0.02046539516117279
32-33	0.025441213516737695
34-35	0.015781047336896215
36-37	0.010034914005783609
38-39	0.014053043472829742
40-41	0.010222287918754671
42-43	0.00920214105924555
44-45	0.009597708208851127
46-47	0.008244452170726781
48-49	0.004372057969324808
50-51	0.0068911961326024364
52-53	0.007557414489832883
54-55	0.0031437178731811718
56-57	0.0029563439602101086
58-59	0.0031437178731811718
60-61	0.00368502028843091
62-63	0.0029979826075370113
64-65	0.002394222221296919
66-67	0.001811281158720278
68-69	0.0028730666655563024
70-71	0.002186028984662404
72-73	0.0019361971007009868
74-75	0.002310944926643113
76-77	0.0035392850227867496
78-79	0.0032894531388253318
80-81	0.0035809236701136522
82-83	0.0022068483083258557
84-85	0.002935524636546657
86-87	0.0023317642503065646
88-89	0.5937879302052994
90-91	0.6038853021820733
92-93	0.6006583070142384
94-95	0.5878336036375522
96-97	0.585564297358236
98-99	0.5830659785186219
100	0.5850646335903131
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2401615.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.44409800622631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.67351808074066	61.54555482522181
2	11.76678692321264	17.51935677860048
3	2.957270452002009	6.604539940792642
4	1.0648740062325852	3.170943395370456
5	0.4870236017490651	1.812801636997637
6	0.27133899178827436	1.211975189855816
7	0.17893540912559866	0.9324479593611188
8	0.1169058042876133	0.6962357721507038
9	0.08753291690937297	0.5864678140655003
>10	0.37687147908667357	4.771810807454283
>50	0.015571078381011296	0.7621274683717514
>100	0.00331528558481722	0.3645715598087462
>500	5.597089965732653E-5	0.021166851949100306
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.163864732690294E-5	0.0
3	0.0	0.0	0.0	4.163864732690294E-5	0.0
4	0.0	0.0	0.0	8.327729465380588E-5	0.0
5	0.0	0.0	0.0	8.327729465380588E-5	0.0
6	0.0	0.0	0.0	8.327729465380588E-5	0.0
7	0.0	0.0	0.0	1.2491594198070881E-4	0.0
8	0.0	0.0	0.0	1.2491594198070881E-4	0.0
9	0.0	0.0	0.0	3.331091786152235E-4	0.0
10-11	0.0	0.0	0.0	3.955671496055779E-4	0.0
12-13	0.0	0.0	0.0	4.372057969324808E-4	0.0
14-15	0.0	0.0	0.0	4.580251205959323E-4	0.0
16-17	0.0	0.0	0.0	4.788444442593838E-4	0.0
18-19	0.0	0.0	0.0	5.829410625766411E-4	0.0
20-21	0.0	0.0	0.0	6.66218357230447E-4	0.0
22-23	0.0	0.0	0.0	6.870376808938985E-4	0.0
24-25	0.0	0.0	0.0	8.535922702015103E-4	0.0
26-27	0.0	0.0	0.0	0.0012283400961436365	0.0
28-29	0.0	0.0	0.0	0.0022484869556527584	0.0
30-31	0.0	0.0	0.0	0.005454662799824286	0.0
32-33	4.163864732690294E-5	0.0	0.0	0.01261651014005159	0.0
34-35	4.163864732690294E-5	0.0	0.0	0.02177701255197024	0.0
36-37	4.163864732690294E-5	0.0	0.0	0.03866148404302938	0.0
38-39	4.163864732690294E-5	0.0	0.0	0.06820410432146701	0.0
40-41	4.163864732690294E-5	0.0	0.0	0.10365941252032487	0.0
42-43	4.163864732690294E-5	0.0	0.0	0.13944782989779794	0.0
44-45	6.245797099035441E-5	0.0	0.0	0.18062845210410494	0.0
46-47	8.327729465380588E-5	0.0	0.0	0.23205218155283008	0.0
48-49	1.0409661831725734E-4	0.0	0.0	0.2912415187280226	0.0
50-51	1.2491594198070881E-4	0.0	0.0	0.3578425351274038	0.0
52-53	1.2491594198070881E-4	0.0	0.0	0.4237148751985643	0.0
54-55	1.2491594198070881E-4	0.0	0.0	0.49566645777945256	0.0
56-57	1.2491594198070881E-4	0.0	0.0	0.5767785427722595	0.0
58-59	1.2491594198070881E-4	2.081932366345147E-5	0.0	0.672859721479088	0.0
60-61	1.2491594198070881E-4	4.163864732690294E-5	0.0	0.7740624538071257	0.0
62-63	1.2491594198070881E-4	4.163864732690294E-5	0.0	0.8807614875823144	0.0
64-65	1.2491594198070881E-4	4.163864732690294E-5	0.0	0.9901878527574153	0.0
66-67	1.2491594198070881E-4	4.163864732690294E-5	0.0	1.1173106430464501	0.0
68-69	1.2491594198070881E-4	4.163864732690294E-5	0.0	1.2599230101410925	0.0
70-71	1.2491594198070881E-4	4.163864732690294E-5	0.0	1.4126743878598358	0.0
72-73	1.2491594198070881E-4	4.163864732690294E-5	0.0	1.584267253494003	0.0
74-75	1.2491594198070881E-4	4.163864732690294E-5	0.0	1.7533409809648925	0.0
76-77	1.2491594198070881E-4	4.163864732690294E-5	0.0	1.928702144182144	0.0
78-79	1.2491594198070881E-4	4.163864732690294E-5	0.0	2.1303789325099984	0.0
80-81	1.2491594198070881E-4	4.163864732690294E-5	0.0	2.361723257058271	0.0
82-83	1.2491594198070881E-4	4.163864732690294E-5	0.0	2.6081615912625464	0.0
84-85	1.2491594198070881E-4	4.163864732690294E-5	0.0	2.8487288761937277	0.0
86-87	1.2491594198070881E-4	4.163864732690294E-5	0.0	3.0923149630561104	0.0
88	1.2491594198070881E-4	4.163864732690294E-5	0.0	3.2944081378572334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	2980	0.0	31.842958	4
GTATCAA	3330	0.0	31.114428	1
ATCAACG	3075	0.0	31.019066	3
CAACGCA	3205	0.0	29.607492	5
AACGCAG	3280	0.0	29.066444	6
GGTATCA	2135	0.0	27.13255	1
GTACATG	6300	0.0	26.538185	1
TATCAAC	3905	0.0	24.91823	2
TACATGG	6240	0.0	24.859777	2
ACGCAGA	3990	0.0	23.776516	7
ACATGGG	6430	0.0	23.383892	3
CGCAGAG	4000	0.0	23.130018	8
TCTGTCG	605	0.0	20.183092	8
GAGTACA	4520	0.0	19.901157	1
CTGTCGC	680	0.0	19.338324	9
CCGGTCG	220	1.500397E-6	19.21275	9
CATGGGG	4025	0.0	18.790497	4
AGTACAT	4555	0.0	18.36959	2
GCAGAGT	4850	0.0	18.01113	9
GTATAGA	980	0.0	17.781116	1
>>END_MODULE