FastQCFastQC Report
Wed 25 May 2016
SRR1033150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401615
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46570.19391118060138698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37390.1556869023552901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37300.046.9378551
GTATCAA68400.041.490661
ATCAACG78650.035.6288833
TCAACGC78950.035.553054
CAACGCA80850.034.71615
AACGCAG83700.033.5754936
TATCAAC83850.033.363272
ACGCAGA96700.028.8673327
CGCAGAG97200.028.4281568
GTACATG59600.025.7430781
GCAGAGT108550.025.1959539
TACATGG60750.024.3017792
ACATGGG60900.022.8522573
GAGTACT73850.021.73090712-13
TATAACG1954.66227E-721.7001442
AGAGTAC97250.021.09270510-11
GTCTCGC5900.019.9423871
CAGAGTA108300.019.65655310-11
AGTACTT80750.019.43755312-13
CATGGGG37700.019.3305854
Produced by FastQC (version 0.11.5)