FastQCFastQC Report
Wed 25 May 2016
SRR1033149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370713
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27200.030.6535741
TCAACGC35000.029.9299834
ATCAACG35200.029.76393
GTATCAA37900.029.5811121
CAACGCA37750.027.7500655
GTACATG80450.026.5831571
AACGCAG40100.026.2323936
TACATGG79750.025.2908732
ACATGGG81600.023.5483683
TATCAAC46150.023.533012
CTGTCGC8950.021.512719
ACGCAGA48350.021.464967
CGCAGAG49950.020.5893658
GTATTAG17500.018.0348911
CATGGGG55700.017.457614
GAGTACA56700.017.4466881
GGGTACG1901.989755E-417.3548641
CGTGTCG2454.1990534E-617.250869
GTCTCGC7000.016.8235931
GTATATA30650.016.5985451
Produced by FastQC (version 0.11.5)