##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033149_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3370713
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.236819331696292	33.0	31.0	34.0	28.0	34.0
2	31.420527645041272	33.0	31.0	34.0	28.0	34.0
3	31.50947796504775	34.0	31.0	34.0	28.0	34.0
4	34.911716897878875	37.0	35.0	37.0	32.0	37.0
5	34.992623815792086	37.0	35.0	37.0	32.0	37.0
6	35.071693436967195	37.0	35.0	37.0	33.0	37.0
7	34.98534078694923	37.0	35.0	37.0	32.0	37.0
8	34.968298398588075	37.0	35.0	37.0	32.0	37.0
9	36.61003057810024	39.0	38.0	39.0	33.0	39.0
10-11	36.59361342837554	39.0	37.5	39.0	33.0	39.0
12-13	36.53587623746074	39.0	37.0	39.0	33.0	39.0
14-15	37.9330086839194	41.0	38.5	41.0	33.0	41.0
16-17	37.93394112758933	41.0	38.5	41.0	33.0	41.0
18-19	37.918476298634744	41.0	38.5	41.0	33.0	41.0
20-21	37.93237528677167	40.5	39.0	41.0	33.0	41.0
22-23	37.88485685372798	40.0	38.0	41.0	33.0	41.0
24-25	37.875522626815155	40.0	38.0	41.0	32.5	41.0
26-27	37.825435597750385	40.0	38.0	41.0	32.0	41.0
28-29	37.79080271740727	40.0	38.0	41.0	32.0	41.0
30-31	37.78620280041641	40.0	38.0	41.0	32.0	41.0
32-33	37.853096511034906	40.0	38.0	41.0	32.0	41.0
34-35	37.82666115447978	40.0	38.0	41.0	32.0	41.0
36-37	37.74429890649249	40.0	38.0	41.0	32.0	41.0
38-39	37.66095229703626	40.0	38.0	41.0	32.0	41.0
40-41	37.601336126807595	40.0	38.0	41.0	32.0	41.0
42-43	37.486200100690866	40.0	38.0	41.0	31.0	41.0
44-45	37.36632531455511	40.0	38.0	41.0	31.0	41.0
46-47	37.24165718054311	40.0	38.0	41.0	31.0	41.0
48-49	37.12430456108248	40.0	37.5	41.0	30.5	41.0
50-51	37.004946431215004	40.0	37.0	41.0	30.0	41.0
52-53	36.85616188622407	40.0	37.0	41.0	30.0	41.0
54-55	36.70358912194541	40.0	36.5	41.0	30.0	41.0
56-57	36.550194869750115	40.0	36.0	41.0	30.0	41.0
58-59	36.35606442909854	40.0	36.0	41.0	30.0	41.0
60-61	36.13851238595514	39.0	35.0	41.0	29.5	41.0
62-63	35.879900928972596	39.0	35.0	41.0	29.0	41.0
64-65	35.61297758070771	39.0	35.0	41.0	29.0	41.0
66-67	35.326773593598745	38.0	35.0	40.0	28.5	41.0
68-69	35.01976614443294	37.5	35.0	40.0	28.0	41.0
70-71	34.669455542492045	37.0	34.0	40.0	28.0	41.0
72-73	34.30215506333526	36.5	34.0	39.0	28.0	41.0
74-75	33.92567878072087	36.0	34.0	39.0	27.0	41.0
76-77	33.59057371541273	35.5	34.0	38.5	27.0	40.0
78-79	33.21468425226354	35.0	34.0	37.0	26.0	39.5
80-81	32.84507031598359	35.0	33.0	37.0	26.0	39.0
82-83	32.61110334816402	35.0	33.0	36.5	26.0	39.0
84-85	32.324774610000915	35.0	33.0	36.0	26.0	37.5
86-87	32.055022631710266	35.0	33.0	36.0	26.0	37.0
88-89	31.68271267829685	35.0	33.0	35.5	24.5	37.0
90-91	31.47814408998927	35.0	33.0	35.0	24.0	36.0
92-93	31.288295236052434	35.0	33.0	35.0	23.5	36.0
94-95	31.106153801881085	35.0	33.0	35.0	22.5	36.0
96-97	30.91322132735715	35.0	33.0	35.0	20.0	36.0
98-99	30.70468340081164	35.0	33.0	35.0	18.5	35.0
100	30.532831184381465	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15242930472608762
1101	2	0.2115944912236074
1101	3	0.20383985700722462
1101	4	0.14954018647297573
1101	5	0.12629892002879473
1101	6	0.1426838065140359
1101	7	0.17666763956722065
1101	8	0.09776035422601836
1101	9	0.1949282900607514
1101	10-11	0.1923706775018985
1101	12-13	0.20116742791589104
1101	14-15	0.262432710277551
1101	16-17	0.2215064679412322
1101	18-19	0.21982712499158197
1101	20-21	0.20333852354573168
1101	22-23	0.2230057294675163
1101	24-25	0.19978523794676306
1101	26-27	0.21300752209728557
1101	28-29	0.28561741520130823
1101	30-31	0.31302576809497396
1101	32-33	0.3207928007419554
1101	34-35	0.27845455465043756
1101	36-37	0.28138438019308154
1101	38-39	0.24210614058519297
1101	40-41	0.2181495956991597
1101	42-43	0.22109988701056693
1101	44-45	0.2514579817671674
1101	46-47	0.23692912405455502
1101	48-49	0.21348830281905862
1101	50-51	0.22189578789864584
1101	52-53	0.22970519736091433
1101	54-55	0.15246421746687133
1101	56-57	0.2139716765493631
1101	58-59	0.19467993197719125
1101	60-61	0.21894221323729113
1101	62-63	0.22666927392747027
1101	64-65	0.21696757573999292
1101	66-67	0.20986527743600902
1101	68-69	0.22261935884966988
1101	70-71	0.2246043916419893
1101	72-73	0.3262391521047263
1101	74-75	0.27755727152759846
1101	76-77	0.2514539987909856
1101	78-79	0.21534470492518665
1101	80-81	0.18396592852715088
1101	82-83	0.18955551015866234
1101	84-85	0.16495637214286774
1101	86-87	0.19026119548181697
1101	88-89	0.3545777556755958
1101	90-91	0.42093965543335443
1101	92-93	0.4773297566604846
1101	94-95	0.49708892099276625
1101	96-97	0.45818606291024366
1101	98-99	0.5030459330576207
1101	100	0.48419631162109056
1102	1	0.43135103339525216
1102	2	0.4118238043662501
1102	3	0.3980187572342935
1102	4	0.36257907678525925
1102	5	0.2913655176765886
1102	6	0.3137418204836493
1102	7	0.3516664023163827
1102	8	0.2675508268446549
1102	9	0.39728311160630625
1102	10-11	0.40372821149274785
1102	12-13	0.4001093943739491
1102	14-15	0.44905002733428745
1102	16-17	0.4617532032533447
1102	18-19	0.4751639866878108
1102	20-21	0.4390332475819463
1102	22-23	0.43712841423567284
1102	24-25	0.4986531743978304
1102	26-27	0.5234409345618545
1102	28-29	0.4872510196983981
1102	30-31	0.4165372723841614
1102	32-33	0.41340547654691306
1102	34-35	0.44482651018620345
1102	36-37	0.49547064644779226
1102	38-39	0.4657496220254558
1102	40-41	0.438345434565953
1102	42-43	0.5104297611986937
1102	44-45	0.42504921440733057
1102	46-47	0.4377919981691534
1102	48-49	0.46100361886605157
1102	50-51	0.4531475697961582
1102	52-53	0.4194687198135938
1102	54-55	0.4572517118293433
1102	56-57	0.39843622374398535
1102	58-59	0.5182865941372512
1102	60-61	0.44966230347877456
1102	62-63	0.520858934275779
1102	64-65	0.5310299311319326
1102	66-67	0.49148092758266415
1102	68-69	0.45631640043612265
1102	70-71	0.4819435725660881
1102	72-73	0.48685840383964063
1102	74-75	0.46442043102540254
1102	76-77	0.5103186331104368
1102	78-79	0.5197299879126405
1102	80-81	0.48548533314060904
1102	82-83	0.5030921154946597
1102	84-85	0.4193798351534497
1102	86-87	0.41003606615765165
1102	88-89	0.5084367946387935
1102	90-91	0.5054177215156948
1102	92-93	0.618631538404216
1102	94-95	0.599908799731935
1102	96-97	0.4892472103166341
1102	98-99	0.5465835319223551
1102	100	0.584834434809892
1103	1	0.2471032708173233
1103	2	0.24147734164439072
1103	3	0.2501634309559897
1103	4	0.2702818050325959
1103	5	0.27989975212952345
1103	6	0.2780027421673168
1103	7	0.2820470888092075
1103	8	0.1522630252671675
1103	9	0.24073207554081932
1103	10-11	0.3244022095667418
1103	12-13	0.372542857192002
1103	14-15	0.3979390753760157
1103	16-17	0.382606856744097
1103	18-19	0.3807641020350587
1103	20-21	0.3550719691384927
1103	22-23	0.3558334592536383
1103	24-25	0.33811695210683723
1103	26-27	0.38575195946400953
1103	28-29	0.40782342637515967
1103	30-31	0.4238988626690343
1103	32-33	0.3660097769729589
1103	34-35	0.42299930101727057
1103	36-37	0.3976325871012847
1103	38-39	0.4139540549259664
1103	40-41	0.4020359976265837
1103	42-43	0.3967664304016978
1103	44-45	0.3279136410933603
1103	46-47	0.3199274754806041
1103	48-49	0.33595274429212196
1103	50-51	0.2600202728908343
1103	52-53	0.27462911207717866
1103	54-55	0.33080418090143127
1103	56-57	0.260862113630985
1103	58-59	0.2416761383722843
1103	60-61	0.29812564119877294
1103	62-63	0.34359253792455746
1103	64-65	0.3753322357602684
1103	66-67	0.34737979555522003
1103	68-69	0.30156004806667625
1103	70-71	0.35684636438242023
1103	72-73	0.3371244705371481
1103	74-75	0.3745071992732534
1103	76-77	0.24798145315601516
1103	78-79	0.22834463191142618
1103	80-81	0.31606919341358974
1103	82-83	0.33085818917825804
1103	84-85	0.3758040216943712
1103	86-87	0.4250048869735892
1103	88-89	0.46968107381267643
1103	90-91	0.4392398053220141
1103	92-93	0.47010638047105857
1103	94-95	0.48319434664746197
1103	96-97	0.41492769464528223
1103	98-99	0.504174306810512
1103	100	0.5889983299484598
1104	1	0.13223511837279034
1104	2	0.18944729964351836
1104	3	0.1473884597496884
1104	4	0.1523086745540354
1104	5	0.11618119243518521
1104	6	0.15638488110812432
1104	7	0.1413647815890542
1104	8	0.1199070110048055
1104	9	0.21286891285469522
1104	10-11	0.19723638889459494
1104	12-13	0.16700351844706063
1104	14-15	0.13761218068164993
1104	16-17	0.2509590898937546
1104	18-19	0.20149828101807543
1104	20-21	0.15260174561662865
1104	22-23	0.16488200853396506
1104	24-25	0.1493955906303981
1104	26-27	0.17657681273684034
1104	28-29	0.19215482633516245
1104	30-31	0.1812616012084476
1104	32-33	0.20010940489628837
1104	34-35	0.2050764733035919
1104	36-37	0.2597006385092442
1104	38-39	0.17772132252460437
1104	40-41	0.19036515271254473
1104	42-43	0.21315207113909196
1104	44-45	0.1285394730071303
1104	46-47	0.13234199957420145
1104	48-49	0.035908777865230945
1104	50-51	0.06265838781735056
1104	52-53	0.06623742983548198
1104	54-55	0.07509130408328701
1104	56-57	0.09712588115721132
1104	58-59	0.12706674128131112
1104	60-61	0.16263378619488122
1104	62-63	0.17155893550520318
1104	64-65	0.07018859485735618
1104	66-67	0.10981380281054243
1104	68-69	0.016159315637722216
1104	70-71	0.01146900312667043
1104	72-73	0.10061410308126284
1104	74-75	0.03492090610856735
1104	76-77	-0.034973323494533304
1104	78-79	-0.05473980259412059
1104	80-81	0.031399639905586696
1104	82-83	0.021762225123836743
1104	84-85	0.02959522082262822
1104	86-87	0.04879020666985667
1104	88-89	0.193362081005926
1104	90-91	0.14755101176076835
1104	92-93	0.14964630198667805
1104	94-95	0.19668242743534137
1104	96-97	0.32392878604717623
1104	98-99	0.33598746501378685
1104	100	0.3295521862351407
1105	1	0.11858955250275116
1105	2	0.15656165871916627
1105	3	0.16326590395273044
1105	4	0.1389338638357529
1105	5	0.05574930093475672
1105	6	0.09985219297855252
1105	7	0.08579907614432614
1105	8	0.06613948187187901
1105	9	0.05196793188162019
1105	10-11	0.09015149192968863
1105	12-13	0.09948837590969362
1105	14-15	0.1261083785880288
1105	16-17	0.15891097941906906
1105	18-19	0.11755142803235685
1105	20-21	0.16588767622430112
1105	22-23	0.19812399286473692
1105	24-25	0.19606299796465265
1105	26-27	0.25099374275198016
1105	28-29	0.2708268916455765
1105	30-31	0.2601394075156449
1105	32-33	0.29838817571728526
1105	34-35	0.2602088882091209
1105	36-37	0.2761364637775259
1105	38-39	0.2629351285684507
1105	40-41	0.22531396435255147
1105	42-43	0.21714221203052375
1105	44-45	0.23778999970407
1105	46-47	0.2322222583140814
1105	48-49	0.22697797267726827
1105	50-51	0.2762080783828935
1105	52-53	0.2520227843683642
1105	54-55	0.20009624186721453
1105	56-57	0.19819491137644718
1105	58-59	0.205226511509764
1105	60-61	0.22913339117216225
1105	62-63	0.31021231953311457
1105	64-65	0.26066114077875113
1105	66-67	0.26974738961678923
1105	68-69	0.19680649451717613
1105	70-71	0.18332404624998588
1105	72-73	0.16960111670955058
1105	74-75	0.18833085738909006
1105	76-77	0.24909039037734715
1105	78-79	0.1924946269272425
1105	80-81	0.24996611893645593
1105	82-83	0.2470774203308892
1105	84-85	0.23887413977713834
1105	86-87	0.2590081275336402
1105	88-89	0.29049816633083125
1105	90-91	0.30704637024387793
1105	92-93	0.2461964369527685
1105	94-95	0.25283120286492533
1105	96-97	0.3038167806485319
1105	98-99	0.2822964715316587
1105	100	0.3213595797436035
1106	1	0.0026390996374274778
1106	2	-0.009342708162879632
1106	3	-0.03952606015663562
1106	4	-0.013187031925276926
1106	5	-0.07577821427831566
1106	6	-0.06264322193637639
1106	7	-0.015026709433584529
1106	8	-0.10446847689527061
1106	9	-0.10799369448332641
1106	10-11	-0.04584518799845938
1106	12-13	-0.09752614587769415
1106	14-15	-0.057513697538496444
1106	16-17	-0.05453954398602434
1106	18-19	-0.035655309926497125
1106	20-21	-0.036527840644112075
1106	22-23	0.019809799322416666
1106	24-25	0.11116861951408197
1106	26-27	0.0840660789351233
1106	28-29	0.05234217996513024
1106	30-31	0.13024676904607446
1106	32-33	0.12798313635609304
1106	34-35	0.07190414699601888
1106	36-37	0.04295102882930735
1106	38-39	0.08852343025293408
1106	40-41	0.10023049229100423
1106	42-43	0.1666307986956781
1106	44-45	0.09841164318667239
1106	46-47	0.10603930636577275
1106	48-49	0.1194763032360342
1106	50-51	0.15893571022171926
1106	52-53	0.08229422140456677
1106	54-55	0.09230441883738649
1106	56-57	0.08583587335081688
1106	58-59	0.0794428146614834
1106	60-61	0.10983323974679138
1106	62-63	0.21147404089161626
1106	64-65	0.2128408196817162
1106	66-67	0.20378823684645653
1106	68-69	0.16116321883835383
1106	70-71	0.15275022873166932
1106	72-73	0.16428994226939864
1106	74-75	0.22943203023034187
1106	76-77	0.16962757424160912
1106	78-79	0.1673474339398382
1106	80-81	0.14010865629902725
1106	82-83	0.05412951630259499
1106	84-85	0.12382824189991481
1106	86-87	0.15983626443722443
1106	88-89	0.22577816219250124
1106	90-91	0.22434648404804491
1106	92-93	0.23102803500775337
1106	94-95	0.18403117841113925
1106	96-97	0.16527831766310186
1106	98-99	0.27657099350714986
1106	100	0.3319819286239003
1107	1	0.05605614594116304
1107	2	0.09475210886612118
1107	3	0.06507163919581771
1107	4	0.06795967526380764
1107	5	-0.0026460964089594086
1107	6	0.033423977733441745
1107	7	0.04846882820233844
1107	8	0.010104010595206603
1107	9	0.0359489446222625
1107	10-11	0.057169343997884425
1107	12-13	0.04322071514648229
1107	14-15	0.05880923733485588
1107	16-17	0.035314323100870126
1107	18-19	0.0968290381868826
1107	20-21	0.03524910055983099
1107	22-23	0.05387564720285809
1107	24-25	0.11151086611089767
1107	26-27	0.14431086016306693
1107	28-29	0.08867712147965534
1107	30-31	0.048834680693040866
1107	32-33	0.09071395413133843
1107	34-35	0.13511189524010092
1107	36-37	0.03167031001505194
1107	38-39	0.09800914578934794
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2216	34-35	-0.5181526891076231
2216	36-37	-0.48818543756940613
2216	38-39	-0.489675992312371
2216	40-41	-0.5066065979250354
2216	42-43	-0.5150559647992594
2216	44-45	-0.48170099381788845
2216	46-47	-0.4202195487861573
2216	48-49	-0.43294820408389256
2216	50-51	-0.4094235545925855
2216	52-53	-0.41043083931111823
2216	54-55	-0.3793922353596031
2216	56-57	-0.5265196463918258
2216	58-59	-0.5127892324915138
2216	60-61	-0.48745163887326015
2216	62-63	-0.43087349042083645
2216	64-65	-0.36002977525276947
2216	66-67	-0.40670040941129315
2216	68-69	-0.38201487855074134
2216	70-71	-0.5070526617210973
2216	72-73	-0.38689242599097184
2216	74-75	-0.413449707376202
2216	76-77	-0.5246327685452812
2216	78-79	-0.5249661024266246
2216	80-81	-0.48769920951869494
2216	82-83	-0.46904870753407835
2216	84-85	-0.5275029384896541
2216	86-87	-0.5135606095006473
2216	88-89	-0.4462259819532939
2216	90-91	-0.44901943802300437
2216	92-93	-0.4138906897669976
2216	94-95	-0.42633247927552276
2216	96-97	-0.4477861422570477
2216	98-99	-0.49230603246796534
2216	100	-0.5188098555625906
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53508.0
3	9047.0
4	5569.0
5	4335.0
6	3715.0
7	4054.0
8	4860.0
9	5108.0
10	5600.0
11	6334.0
12	6799.0
13	7359.0
14	7912.0
15	8195.0
16	8358.0
17	8282.0
18	8276.0
19	8518.0
20	8666.0
21	8856.0
22	10168.0
23	13710.0
24	20113.0
25	26078.0
26	26265.0
27	24024.0
28	26457.0
29	32289.0
30	39044.0
31	48131.0
32	62275.0
33	83296.0
34	114491.0
35	171422.0
36	308225.0
37	655041.0
38	1069222.0
39	456213.0
40	898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21364319893891	19.636387340738263	13.014188745920602	24.135780714402227
2	15.298568530846849	19.950538824438667	40.79017287352143	23.960719771193055
3	18.20554070056889	25.218295076341306	29.850042645208198	26.726121577881614
4	11.827265755402175	16.533170454896208	39.55194777442998	32.087616015271635
5	12.524471306984458	38.21619754176891	36.21691105325455	13.042420097992077
6	30.96990325771615	38.60093080995182	17.812911116135247	12.616254816196781
7	27.157707101311505	32.605097914953255	22.72317025844421	17.514024725291026
8	24.64212160136063	37.128765604744395	20.449147219059707	17.77996557483527
9	24.662794406735657	16.887533845183782	21.099187715589185	37.35048403249138
10-11	23.495261241855967	27.142811996990527	29.33800114746804	20.02392561368546
12-13	24.253660429793676	24.434777358069564	29.169646832375008	22.141915379761755
14-15	21.38120018248649	26.57007740839512	26.11668008115456	25.932042327963828
16-17	21.206566082401988	29.927822113236896	26.754036592344416	22.111575212016703
18-19	21.1330842390498	29.029182131204163	28.565869137669296	21.271864492076737
20-21	22.349468763905175	28.32715638797142	28.006407267108585	21.31696758101482
22-23	21.816286325229502	28.17760868768592	28.08015143335157	21.92595355373301
24-25	21.82049264948733	28.24704642658642	28.037826630515255	21.894634293410995
26-27	21.539239193215263	28.190048733069272	28.148684895537762	22.122027178177696
28-29	21.57601015402396	28.261702015597496	28.226287557389	21.93600027298954
30-31	21.84760755423761	28.749912278892737	27.802405628037874	21.60007453883178
32-33	21.422109815375908	28.37763952447868	28.358425313024725	21.84182534712068
34-35	21.59108172680348	28.123980032069273	28.42838297028904	21.856555270838204
36-37	21.875081592882964	28.522424097849747	27.83105051281869	21.771443796448605
38-39	21.807345097951945	28.546172048274993	28.022535253057985	21.623947600715073
40-41	21.541013621979715	28.294873736740627	28.344660134402506	21.819452506877155
42-43	21.5044042593372	28.180999430191484	28.280408096235774	22.034188214235538
44-45	21.62258188411914	28.326422964981944	28.244608199248848	21.80638695165007
46-47	21.666466149039437	28.524762394029153	28.07005962271731	21.738711834214104
48-49	21.617315272905337	28.354803715154116	28.147554118015822	21.880326893924728
50-51	21.623890112413623	28.258234166513624	28.151232206269754	21.966643514803
52-53	21.65582966310223	28.136936842850126	28.235661843951597	21.97157165009605
54-55	21.715479731585997	28.658561911865316	27.971980772642613	21.653977583906077
56-57	21.551933348918954	28.354256239490088	28.072825004717224	22.020985406873734
58-59	21.450668813840966	27.971405358313422	28.46822620627618	22.10969962156943
60-61	21.647746953252422	28.456900509626987	28.061882111580942	21.833470425539648
62-63	21.574360371040868	28.41964667690809	28.06656686324736	21.939426088803682
64-65	21.521931230536882	28.134966119920602	28.266691904219332	22.076410745323177
66-67	21.37888844815714	28.236898622434737	28.069690864421982	22.314522064986146
68-69	21.82884948041065	28.30278160128725	28.052202660391217	21.816166257910883
70-71	21.7199468051572	28.120209070862916	28.126943721903412	22.032900402076468
72-73	21.52662268800123	28.268483566742848	28.240729271910354	21.96416447334557
74-75	21.59038635980986	28.23536567004215	28.143275992378275	22.030971977769717
76-77	21.551883989346663	28.292015604403627	28.023531212812486	22.132569193437227
78-79	21.72846981867291	28.263801573253854	28.1510321326152	21.856696475458033
80-81	21.634367838309206	28.02534221468907	28.280520735071974	22.05976921192975
82-83	21.452211502360754	28.172392263364937	28.406858225822795	21.968538008451517
84-85	21.4773004569651	28.063739251752096	28.233219085876954	22.22574120540585
86-87	21.56286459163882	28.242995575762748	28.159361545108307	22.034778287490127
88-89	21.452506629775712	27.990129580962357	28.263528547956003	22.293835241305928
90-91	21.407013140683702	28.004056054021813	28.290543973321025	22.298386831973456
92-93	21.567612284923324	28.152334887930365	28.213451162127072	22.06660166501924
94-95	21.349349974340406	27.887107333212263	28.35584448569412	22.407698206753214
96-97	21.332942766312787	27.948517377347432	28.337214132994937	22.381325723344844
98-99	21.353829836190528	27.95212342906121	28.29408827727728	22.399958457470987
100	21.54952645913677	27.8654340537569	28.166832501968237	22.41820698513809
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	266.0
1	252.5
2	230.5
3	210.0
4	181.0
5	149.0
6	129.5
7	119.5
8	133.5
9	151.5
10	166.0
11	186.5
12	198.0
13	221.0
14	263.5
15	320.5
16	402.5
17	596.0
18	903.0
19	1294.5
20	1924.5
21	2841.5
22	4356.0
23	6920.0
24	10443.5
25	14903.5
26	20975.5
27	28818.5
28	38691.5
29	49383.0
30	59169.0
31	68792.0
32	77863.0
33	87644.0
34	97521.5
35	105396.0
36	111463.5
37	116611.5
38	120265.0
39	122004.5
40	123788.0
41	124636.0
42	124305.0
43	123116.0
44	120775.5
45	118918.0
46	115932.5
47	111412.5
48	108792.0
49	106145.0
50	102317.0
51	98192.5
52	92690.0
53	88135.5
54	84497.5
55	80651.0
56	74733.0
57	67773.5
58	60783.5
59	52504.0
60	45047.0
61	38523.0
62	32233.5
63	26437.5
64	21498.0
65	17276.5
66	13801.0
67	10940.0
68	8617.0
69	6801.5
70	5152.0
71	3555.0
72	2538.0
73	1876.5
74	1407.0
75	961.0
76	544.0
77	354.0
78	257.0
79	172.5
80	115.5
81	68.5
82	45.5
83	29.5
84	23.5
85	19.0
86	13.5
87	14.0
88	13.5
89	7.0
90	3.0
91	3.0
92	4.5
93	6.5
94	4.5
95	3.0
96	4.0
97	4.0
98	3.5
99	2.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3307608805614717
2	0.11961860888185971
3	0.06592077106535027
4	0.03942785992162489
5	0.043343945331447684
6	0.00934520381889529
7	0.007031153349454552
8	0.014952326110232464
9	0.017444380461937876
10-11	0.02030727623502802
12-13	0.015263832904195642
14-15	0.01781522188332261
16-17	0.018883245176910642
18-19	0.01889807883376603
20-21	0.023422344174659784
22-23	0.015352834845327977
24-25	0.024089858733152305
26-27	0.018378900843827404
28-29	0.018408568157538184
30-31	0.019506258764836994
32-33	0.024075025076296913
34-35	0.015100662678786358
36-37	0.009523207701159962
38-39	0.012994283405321069
40-41	0.009330370162039902
42-43	0.008381016123294983
44-45	0.008737023887824326
46-47	0.007995341045054859
48-49	0.004138590262653628
50-51	0.006348805134106642
52-53	0.007105321633731497
54-55	0.002937064057367091
56-57	0.0025217216654161894
58-59	0.002417886067428464
60-61	0.0032782381650410465
62-63	0.0025810562928377466
64-65	0.002210214871453013
66-67	0.001661369567803607
68-69	0.003515576674727276
70-71	0.002536555322271579
72-73	0.0018838744206344474
74-75	0.002536555322271579
76-77	0.003738081527558116
78-79	0.0035304103315826653
80-81	0.0039012517529673986
82-83	0.002210214871453013
84-85	0.0031299015964871527
86-87	0.0022398821851637917
88-89	0.602187133701386
90-91	0.612897033950977
92-93	0.6094111245899606
94-95	0.5967431816354581
96-97	0.5942066263131865
98-99	0.5912695622558195
100	0.5933166069018632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3370713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.25920598952158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.02659860989348	50.26694665355236
2	13.95660273237686	18.215936252522024
3	4.389754679114696	8.594157145434364
4	1.8223230145012377	4.756934119311287
5	0.9306517859326087	3.036679830134612
6	0.4971570605443821	1.946644501392653
7	0.3226506345111018	1.4739146954146882
8	0.2101718687194862	1.097251941917411
9	0.15177384725316614	0.8914176685544897
>10	0.6509517079494204	7.206485397163102
>50	0.03163644241191796	1.4085460744902112
>100	0.009545711973805278	1.0392909024678234
>500	1.8190481778210154E-4	0.06579481764495908
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.9667313710778697E-5	0.0	0.0	0.0
4	0.0	2.9667313710778697E-5	0.0	2.9667313710778697E-5	0.0
5	0.0	2.9667313710778697E-5	0.0	2.9667313710778697E-5	0.0
6	0.0	2.9667313710778697E-5	0.0	2.9667313710778697E-5	0.0
7	0.0	2.9667313710778697E-5	0.0	5.9334627421557395E-5	0.0
8	0.0	2.9667313710778697E-5	0.0	5.9334627421557395E-5	0.0
9	0.0	2.9667313710778697E-5	0.0	2.3733850968622958E-4	0.0
10-11	0.0	7.416828427694675E-5	0.0	3.560077645293444E-4	0.0
12-13	0.0	1.1866925484311479E-4	0.0	3.856750782401231E-4	0.0
14-15	0.0	1.1866925484311479E-4	0.0	5.043443330832379E-4	0.0
16-17	0.0	1.1866925484311479E-4	0.0	6.081799310709633E-4	0.0
18-19	0.0	1.1866925484311479E-4	0.0	6.230135879263526E-4	0.0
20-21	0.0	1.1866925484311479E-4	0.0	6.526809016371314E-4	0.0
22-23	0.0	1.1866925484311479E-4	0.0	6.675145584925208E-4	0.0
24-25	0.0	1.1866925484311479E-4	0.0	7.713501564802462E-4	0.0
26-27	0.0	1.1866925484311479E-4	0.0	9.048530681787504E-4	0.0
28-29	0.0	1.780038822646722E-4	0.0	0.001661369567803607	0.0
30-31	0.0	1.780038822646722E-4	0.0	0.004895106762278485	0.0
32-33	0.0	1.780038822646722E-4	0.0	0.011777923543179143	0.0
34-35	0.0	1.780038822646722E-4	0.0	0.021790641920566955	0.0
36-37	0.0	2.0767119597545089E-4	0.0	0.04111889680313928	0.0
38-39	0.0	2.0767119597545089E-4	0.0	0.0758889884721719	0.0
40-41	0.0	2.0767119597545089E-4	0.0	0.11702271893216658	0.0
42-43	0.0	2.0767119597545089E-4	0.0	0.15814161573530586	0.0
44-45	0.0	2.2250485283084023E-4	0.0	0.20577248789796104	0.0
46-47	0.0	2.3733850968622958E-4	0.0	0.263757252545678	1.4833656855389349E-5
48-49	0.0	2.3733850968622958E-4	0.0	0.33254091938411845	2.9667313710778697E-5
50-51	0.0	2.3733850968622958E-4	0.0	0.4059823544751511	2.9667313710778697E-5
52-53	0.0	2.3733850968622958E-4	0.0	0.48216801608443083	2.9667313710778697E-5
54-55	0.0	2.3733850968622958E-4	0.0	0.5626109372112073	2.9667313710778697E-5
56-57	0.0	2.670058233970083E-4	0.0	0.6590445404280934	2.9667313710778697E-5
58-59	0.0	2.670058233970083E-4	0.0	0.7696146186281656	2.9667313710778697E-5
60-61	0.0	2.670058233970083E-4	0.0	0.8862219951683813	2.9667313710778697E-5
62-63	0.0	2.670058233970083E-4	0.0	1.0063597820401795	2.9667313710778697E-5
64-65	0.0	2.670058233970083E-4	0.0	1.1329057086735062	2.9667313710778697E-5
66-67	0.0	2.96673137107787E-4	0.0	1.2750121413481361	2.9667313710778697E-5
68-69	0.0	2.96673137107787E-4	0.0	1.439472894903838	2.9667313710778697E-5
70-71	0.0	2.96673137107787E-4	0.0	1.6174322761979438	2.9667313710778697E-5
72-73	0.0	2.96673137107787E-4	0.0	1.8045291901149696	2.9667313710778697E-5
74-75	0.0	2.96673137107787E-4	0.0	1.9939698218151471	2.9667313710778697E-5
76-77	0.0	2.96673137107787E-4	0.0	2.195663054077876	2.9667313710778697E-5
78-79	0.0	2.96673137107787E-4	0.0	2.428180625286104	2.9667313710778697E-5
80-81	0.0	2.96673137107787E-4	0.0	2.6895051581074982	2.9667313710778697E-5
82-83	0.0	2.96673137107787E-4	0.0	2.962310941334964	2.9667313710778697E-5
84-85	0.0	2.96673137107787E-4	0.0	3.2302067841432955	2.9667313710778697E-5
86-87	0.0	2.96673137107787E-4	0.0	3.510889239160973	2.9667313710778697E-5
88	0.0	2.96673137107787E-4	0.0	3.7393572220476794	2.9667313710778697E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2720	0.0	30.653574	1
TCAACGC	3500	0.0	29.929983	4
ATCAACG	3520	0.0	29.7639	3
GTATCAA	3790	0.0	29.581112	1
CAACGCA	3775	0.0	27.750065	5
GTACATG	8045	0.0	26.583157	1
AACGCAG	4010	0.0	26.232393	6
TACATGG	7975	0.0	25.290873	2
ACATGGG	8160	0.0	23.548368	3
TATCAAC	4615	0.0	23.53301	2
CTGTCGC	895	0.0	21.51271	9
ACGCAGA	4835	0.0	21.46496	7
CGCAGAG	4995	0.0	20.589365	8
GTATTAG	1750	0.0	18.034891	1
CATGGGG	5570	0.0	17.45761	4
GAGTACA	5670	0.0	17.446688	1
GGGTACG	190	1.989755E-4	17.354864	1
CGTGTCG	245	4.1990534E-6	17.25086	9
GTCTCGC	700	0.0	16.823593	1
GTATATA	3065	0.0	16.598545	1
>>END_MODULE