FastQCFastQC Report
Wed 25 May 2016
SRR1033149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370713
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.13682565083411136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37530.11134142835655246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45100.045.076191
GTATCAA77250.041.9111821
TCAACGC85650.036.9986274
ATCAACG86300.036.828923
CAACGCA87050.036.404675
AACGCAG90750.034.9038286
TATCAAC94600.033.896842
ACGCAGA104400.030.0251457
CGCAGAG107450.029.171148
GTACATG81500.026.5030021
TAGACCG909.500021E-426.1212185
GCAGAGT121750.025.5518889
TACATGG81600.025.4681872
ACATGGG80300.025.0014613
GAGTACT83800.021.50514812-13
AGAGTAC111850.020.67107810-11
CAGAGTA125400.019.67413910-11
GTCGTAC1200.005029686319.6077961
GTGGTAT24000.018.4313261
GTACTTT98550.018.31030814-15
Produced by FastQC (version 0.11.5)