FastQCFastQC Report
Wed 25 May 2016
SRR1033148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374170
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.028.6737941
GTACATG66700.026.9081671
TACCGTC1606.978371E-826.4156117
TCAACGC35600.025.2044374
ATCAACG35450.025.1833443
GTATCAA37850.025.0158421
TACATGG68450.024.5833592
CAACGCA36800.024.5102125
CCGATCG1151.3855791E-424.5014369
AACGCAG38700.023.5412166
ACCGTCC2402.6557245E-1023.4805418
ACATGGG69150.023.170653
GTACCGC3009.094947E-1221.9151736
TATAGCG1354.0917803E-420.8790685
TATCAAC44350.020.8787382
ACGCAGA43650.020.6564227
ATACCGT3202.1827873E-1120.5454756
TATACCG1656.887609E-519.9300215
CGCAGAG45850.019.3580068
GAGTACA49800.018.9184511
Produced by FastQC (version 0.11.5)