##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033148_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2374170 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16195807376894 33.0 31.0 34.0 28.0 34.0 2 31.28728776793574 33.0 31.0 34.0 28.0 34.0 3 31.46825164162634 34.0 31.0 34.0 28.0 34.0 4 34.83638155650185 37.0 35.0 37.0 32.0 37.0 5 34.947014325006215 37.0 35.0 37.0 32.0 37.0 6 34.99956574297544 37.0 35.0 37.0 32.0 37.0 7 34.94635262007354 37.0 35.0 37.0 32.0 37.0 8 34.91546687895138 37.0 35.0 37.0 32.0 37.0 9 36.54556034319362 39.0 38.0 39.0 33.0 39.0 10-11 36.527188870215696 39.0 37.5 39.0 33.0 39.0 12-13 36.464817810013606 39.0 37.0 39.0 32.0 39.0 14-15 37.84824106951061 41.0 38.0 41.0 33.0 41.0 16-17 37.83907049621552 41.0 38.0 41.0 33.0 41.0 18-19 37.81949544472384 40.5 38.5 41.0 32.0 41.0 20-21 37.831080335443545 40.0 38.5 41.0 32.0 41.0 22-23 37.78035144913802 40.0 38.0 41.0 32.0 41.0 24-25 37.761308162431504 40.0 38.0 41.0 32.0 41.0 26-27 37.702465493203945 40.0 38.0 41.0 32.0 41.0 28-29 37.66704342991446 40.0 38.0 41.0 32.0 41.0 30-31 37.66212634310096 40.0 38.0 41.0 32.0 41.0 32-33 37.731703079391956 40.0 38.0 41.0 32.0 41.0 34-35 37.69695325103089 40.0 38.0 41.0 32.0 41.0 36-37 37.61867873825379 40.0 38.0 41.0 32.0 41.0 38-39 37.536625431203326 40.0 38.0 41.0 31.0 41.0 40-41 37.467985653933795 40.0 38.0 41.0 31.0 41.0 42-43 37.35111617954907 40.0 38.0 41.0 31.0 41.0 44-45 37.22480572157849 40.0 38.0 41.0 31.0 41.0 46-47 37.09499572482173 40.0 37.5 41.0 30.0 41.0 48-49 36.9667517490323 40.0 37.0 41.0 30.0 41.0 50-51 36.83950243664101 40.0 37.0 41.0 30.0 41.0 52-53 36.680402835517256 40.0 36.5 41.0 30.0 41.0 54-55 36.51567874246579 40.0 36.0 41.0 30.0 41.0 56-57 36.35305306696656 40.0 36.0 41.0 30.0 41.0 58-59 36.14604345939844 39.0 35.0 41.0 29.5 41.0 60-61 35.9165683165064 39.0 35.0 41.0 29.0 41.0 62-63 35.64574482871909 39.0 35.0 41.0 29.0 41.0 64-65 35.36610120589511 38.5 35.0 40.5 28.0 41.0 66-67 35.07079610979837 37.5 35.0 40.0 28.0 41.0 68-69 34.752693572911795 37.0 34.0 40.0 28.0 41.0 70-71 34.40178883567731 37.0 34.0 39.5 27.5 41.0 72-73 34.033881103712034 36.0 34.0 39.0 27.0 41.0 74-75 33.663582852112526 36.0 34.0 39.0 26.0 40.5 76-77 33.330379669526614 35.0 34.0 38.0 26.0 40.0 78-79 32.95999507196199 35.0 33.0 37.0 26.0 39.0 80-81 32.60207167136305 35.0 33.0 37.0 26.0 39.0 82-83 32.38357383843616 35.0 33.0 36.0 26.0 38.5 84-85 32.111015007349934 35.0 33.0 36.0 25.0 37.0 86-87 31.85358567415139 35.0 33.0 36.0 25.0 37.0 88-89 31.49093641146169 35.0 33.0 35.0 23.5 36.5 90-91 31.29747490702014 35.0 33.0 35.0 23.0 36.0 92-93 31.114149365883655 35.0 33.0 35.0 21.5 36.0 94-95 30.93506235863481 35.0 33.0 35.0 20.0 36.0 96-97 30.74057754920667 35.0 32.5 35.0 18.5 35.5 98-99 30.532267065964106 35.0 32.0 35.0 8.5 35.0 100 30.35804386375028 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3310595838188668 1101 2 0.2628974086679854 1101 3 0.3303326662787498 1101 4 0.3012791124474319 1101 5 0.33124989788323234 1101 6 0.3367247035415488 1101 7 0.24604857167017968 1101 8 0.2549539178512177 1101 9 0.402948463473777 1101 10-11 0.3753152879536543 1101 12-13 0.39429828317945237 1101 14-15 0.45949050851548634 1101 16-17 0.39479508583654876 1101 18-19 0.36846395432383616 1101 20-21 0.3649672146257785 1101 22-23 0.3669624216448284 1101 24-25 0.41887750543961033 1101 26-27 0.4617046935079827 1101 28-29 0.4639744956812706 1101 30-31 0.4456076222620311 1101 32-33 0.4508645002444638 1101 34-35 0.4414926490827469 1101 36-37 0.3790335061215302 1101 38-39 0.4391980601843173 1101 40-41 0.43303228961977425 1101 42-43 0.45005388289666115 1101 44-45 0.36534462694153547 1101 46-47 0.41623833726769277 1101 48-49 0.40884603863685953 1101 50-51 0.4751927166266725 1101 52-53 0.4647015668091399 1101 54-55 0.3977707301019464 1101 56-57 0.4460127417428552 1101 58-59 0.49115251605142873 1101 60-61 0.4842443020390803 1101 62-63 0.4204990396395516 1101 64-65 0.39530235013837967 1101 66-67 0.4393969028042548 1101 68-69 0.4863266462789042 1101 70-71 0.495432056140686 1101 72-73 0.4949929743006649 1101 74-75 0.4649056269624765 1101 76-77 0.4460606516212948 1101 78-79 0.39945813748705206 1101 80-81 0.47456135051719883 1101 82-83 0.5286946319573573 1101 84-85 0.46911950562064675 1101 86-87 0.48551049417957515 1101 88-89 0.6706585068473778 1101 90-91 0.8151617596481167 1101 92-93 0.7095026763933596 1101 94-95 0.7623027852679982 1101 96-97 0.8308822589101688 1101 98-99 0.8575914819158328 1101 100 0.8828362350513217 1102 1 0.333831013501122 1102 2 0.2871522462158431 1102 3 0.293562218397291 1102 4 0.22155030821500787 1102 5 0.10207332455600238 1102 6 0.10444558842689844 1102 7 0.1391978835765002 1102 8 0.1509651491738211 1102 9 0.21769717929286259 1102 10-11 0.2507060109187762 1102 12-13 0.2511736444654815 1102 14-15 0.3364207105109642 1102 16-17 0.2767991215590584 1102 18-19 0.38632969541468043 1102 20-21 0.3211805225980626 1102 22-23 0.3181070227871885 1102 24-25 0.318833542296872 1102 26-27 0.4247720220570699 1102 28-29 0.4039237479446953 1102 30-31 0.3876599704449859 1102 32-33 0.42453297029719295 1102 34-35 0.45875294634878827 1102 36-37 0.46514939877626915 1102 38-39 0.3684107354120485 1102 40-41 0.4222224834036652 1102 42-43 0.44033153428218696 1102 44-45 0.40120774220656585 1102 46-47 0.4636705522111413 1102 48-49 0.45876548226598146 1102 50-51 0.37711739652671383 1102 52-53 0.37472165997341733 1102 54-55 0.4829270385138642 1102 56-57 0.3923219953221775 1102 58-59 0.3617057552463834 1102 60-61 0.39566925046035806 1102 62-63 0.4063720882677444 1102 64-65 0.4038576887977072 1102 66-67 0.379673678344858 1102 68-69 0.41153149786164533 1102 70-71 0.4103784992917312 1102 72-73 0.5266030720663153 1102 74-75 0.5047373451096036 1102 76-77 0.3979001609430668 1102 78-79 0.37081057449350396 1102 80-81 0.36449344903736147 1102 82-83 0.4013492153820124 1102 84-85 0.39383725925358704 1102 86-87 0.43888820080741553 1102 88-89 0.4846077685101058 1102 90-91 0.5185396158104503 1102 92-93 0.5651420282339359 1102 94-95 0.5830686411586115 1102 96-97 0.5462960187329848 1102 98-99 0.4921359592436261 1102 100 0.5508925471802364 1103 1 0.2698632556473086 1103 2 0.21486314476939583 1103 3 0.256958465740297 1103 4 0.24404275088949845 1103 5 0.220510867180721 1103 6 0.2667336889471983 1103 7 0.307539005869522 1103 8 0.22638318572978022 1103 9 0.3739874671948584 1103 10-11 0.3465999403932898 1103 12-13 0.29583193810457686 1103 14-15 0.25497750657467577 1103 16-17 0.26305907856753663 1103 18-19 0.37497963423493985 1103 20-21 0.3227963271719787 1103 22-23 0.3539532941873915 1103 24-25 0.3012316480982662 1103 26-27 0.3250691605574332 1103 28-29 0.3393313272372822 1103 30-31 0.3358544645517654 1103 32-33 0.2782907068650857 1103 34-35 0.3010268873447757 1103 36-37 0.3647702043169616 1103 38-39 0.31517878485013995 1103 40-41 0.3595165836518319 1103 42-43 0.3263105158323327 1103 44-45 0.27138691298338813 1103 46-47 0.2758251515001007 1103 48-49 0.24271757171180752 1103 50-51 0.1678573390925422 1103 52-53 0.15602308546878163 1103 54-55 0.22967648504833704 1103 56-57 0.11450136961509116 1103 58-59 0.13581695864107246 1103 60-61 0.15231824797703553 1103 62-63 0.14445586262201715 1103 64-65 0.16413837447041857 1103 66-67 0.10553066515078768 1103 68-69 0.12074625547680284 1103 70-71 0.1491349426497166 1103 72-73 0.13447143412312101 1103 74-75 0.18251298743022915 1103 76-77 0.14228581462361944 1103 78-79 0.15341949099678232 1103 80-81 0.1702718489844699 1103 82-83 0.0991138192026213 1103 84-85 0.05715155889090795 1103 86-87 0.06434713210807175 1103 88-89 0.24122037628245963 1103 90-91 0.28285514970129455 1103 92-93 0.24214424276222246 1103 94-95 0.18361458724949387 1103 96-97 0.1873286780705179 1103 98-99 0.2237210415469022 1103 100 0.2601325777642778 1104 1 -0.023961863132139882 1104 2 -0.01049739893825219 1104 3 0.020370406501246663 1104 4 0.0014200372246691018 1104 5 0.019237755912925536 1104 6 0.04157988884591646 1104 7 0.04200700194324014 1104 8 0.012678703967353044 1104 9 -0.039668520735993695 1104 10-11 0.037964057296903775 1104 12-13 -0.008640916964559153 1104 14-15 -0.00907993722847067 1104 16-17 0.052534050736049664 1104 18-19 -0.0322500398887513 1104 20-21 0.05140773586251868 1104 22-23 0.01612026356002616 1104 24-25 0.04902911978656732 1104 26-27 0.07679079571111913 1104 28-29 0.1099804557020363 1104 30-31 0.14823932774773851 1104 32-33 0.10724353487891847 1104 34-35 0.1366225452324059 1104 36-37 0.1796623043401766 1104 38-39 0.1241814956665408 1104 40-41 0.05805192415238736 1104 42-43 0.10725861589784103 1104 44-45 0.11156573458809049 1104 46-47 0.07457971551941256 1104 48-49 -0.007153423802314762 1104 50-51 0.051964170667076814 1104 52-53 0.0571323157818 1104 54-55 0.03710230526614566 1104 56-57 0.057457115909848255 1104 58-59 -0.028969150239191777 1104 60-61 -0.025592074800925957 1104 62-63 -0.03864880315102681 1104 64-65 -0.08423174736973493 1104 66-67 0.026253663802982885 1104 68-69 -0.017730828344149074 1104 70-71 -0.00935457782847493 1104 72-73 0.00667937413495423 1104 74-75 0.021785974059099544 1104 76-77 0.0021353972354276607 1104 78-79 -0.061197834678154095 1104 80-81 0.07382171120079306 1104 82-83 0.08705524403693943 1104 84-85 0.12034769649419275 1104 86-87 0.2027608951925579 1104 88-89 0.27634111663123306 1104 90-91 0.1852431705233606 1104 92-93 0.1797644660517257 1104 94-95 0.18463116342478259 1104 96-97 0.18198210097085266 1104 98-99 0.17196432966898811 1104 100 0.1756784153290063 1105 1 0.22183438882532514 1105 2 0.2710654075736265 1105 3 0.27265089525437247 1105 4 0.19307176981929075 1105 5 0.2147936898675269 1105 6 0.24248232442059248 1105 7 0.1897185298931987 1105 8 0.1225360221471874 1105 9 0.22656220254378923 1105 10-11 0.2304179266463251 1105 12-13 0.23743740379277511 1105 14-15 0.31099616251106 1105 16-17 0.3230617951154926 1105 18-19 0.3195808923411363 1105 20-21 0.3225637610943508 1105 22-23 0.3851650067089736 1105 24-25 0.3755692673483466 1105 26-27 0.4336544352315741 1105 28-29 0.45588252095326 1105 30-31 0.47236752749813604 1105 32-33 0.44861161816547224 1105 34-35 0.4534441824589308 1105 36-37 0.48374458398215125 1105 38-39 0.45632783272917976 1105 40-41 0.3731268113998496 1105 42-43 0.4003640930163499 1105 44-45 0.4042306306241059 1105 46-47 0.40283096510150074 1105 48-49 0.3841063427577751 1105 50-51 0.45868869085592934 1105 52-53 0.43759882642808634 1105 54-55 0.36831245079057595 1105 56-57 0.43954451270670347 1105 58-59 0.3417846183499478 1105 60-61 0.32200713323756247 1105 62-63 0.4205444362424302 1105 64-65 0.4355151452011654 1105 66-67 0.47641874038610155 1105 68-69 0.3799306886345377 1105 70-71 0.4195526634140734 1105 72-73 0.37886967470608823 1105 74-75 0.4201730250592135 1105 76-77 0.4239867653210183 1105 78-79 0.4723831192981436 1105 80-81 0.4860601284406485 1105 82-83 0.3704817812080634 1105 84-85 0.39028303871113224 1105 86-87 0.5061175254475394 1105 88-89 0.4482863346017716 1105 90-91 0.49804397180641047 1105 92-93 0.503177053219062 1105 94-95 0.4638563175474033 1105 96-97 0.4761165235396767 1105 98-99 0.5008354384174112 1105 100 0.583575049631019 1106 1 0.15198504978998528 1106 2 0.1371690656202489 1106 3 0.17423892406272756 1106 4 0.17458265019838137 1106 5 0.18599643379094744 1106 6 0.24745870442877305 1106 7 0.19166854989283166 1106 8 0.0939709483039124 1106 9 0.13526219956936814 1106 10-11 0.19341349476250969 1106 12-13 0.20503477493940636 1106 14-15 0.2738063843893741 1106 16-17 0.21868267393634255 1106 18-19 0.24554650184613536 1106 20-21 0.22297145622619752 1106 22-23 0.21547442172074227 1106 24-25 0.2823250156343917 1106 26-27 0.26712661176465247 1106 28-29 0.2838210579210667 1106 30-31 0.2075740120859848 1106 32-33 0.20958611319130682 1106 34-35 0.2381236844080732 1106 36-37 0.2369101201477477 1106 38-39 0.2484714607474956 1106 40-41 0.19927681266509722 1106 42-43 0.2148883347504622 1106 44-45 0.17944593192162017 1106 46-47 0.1707489519439136 1106 48-49 0.1351989976750474 1106 50-51 0.20956581140556807 1106 52-53 0.16606692090449826 1106 54-55 0.21972658709264437 1106 56-57 0.20066284529199407 1106 58-59 0.19545354157931172 1106 60-61 0.15507487762046424 1106 62-63 0.23242865943748114 1106 64-65 0.3357561397047064 1106 66-67 0.3153389864548828 1106 68-69 0.229793798784371 1106 70-71 0.2767898901726511 1106 72-73 0.3078839523596386 1106 74-75 0.3369489017247247 1106 76-77 0.3237318753989271 1106 78-79 0.32747832067294524 1106 80-81 0.32892369895946416 1106 82-83 0.28098718377344056 1106 84-85 0.27669531711139683 1106 86-87 0.36308172720901766 1106 88-89 0.3967369954332476 1106 90-91 0.36276874263189285 1106 92-93 0.38352939752710924 1106 94-95 0.3955311461076825 1106 96-97 0.3460993562605914 1106 98-99 0.3384634771523345 1106 100 0.45234391998676315 1107 1 0.2366191408461269 1107 2 0.265747347192022 1107 3 0.28786737200554313 1107 4 0.2428995395216873 1107 5 0.22436502199238362 1107 6 0.2506431057401386 1107 7 0.19382076048567853 1107 8 0.27114404263819125 1107 9 0.3039446138091364 1107 10-11 0.27470272878132107 1107 12-13 0.25853400666846227 1107 14-15 0.30227066818403614 1107 16-17 0.2475571033170212 1107 18-19 0.336589383368711 1107 20-21 0.26909909108286456 1107 22-23 0.24457051344579384 1107 24-25 0.2494688417490991 1107 26-27 0.365560723830626 1107 28-29 0.3152008517970657 1107 30-31 0.30273618188546436 1107 32-33 0.335895210721155 1107 34-35 0.3457227220483716 1107 36-37 0.3089026128083603 1107 38-39 0.31113507767461357 1107 40-41 0.31613624235341575 1107 42-43 0.2892964534211231 1107 44-45 0.31330444614050634 1107 46-47 0.26536649451727357 1107 48-49 0.31060779952541395 1107 50-51 0.25001059727174635 1107 52-53 0.2668771754880055 1107 54-55 0.2571373763748639 1107 56-57 0.27172812433004623 1107 58-59 0.34461428680285167 1107 60-61 0.3163156982488289 1107 62-63 0.2957495900547613 1107 64-65 0.26149147292016295 1107 66-67 0.39386225051774204 1107 68-69 0.35696530080991096 1107 70-71 0.35575406524360886 1107 72-73 0.36005666843052353 1107 74-75 0.41328782081944127 1107 76-77 0.3851731972370942 1107 78-79 0.35134738880056204 1107 80-81 0.2331183373029191 1107 82-83 0.30105701047404665 1107 84-85 0.277962103179604 1107 86-87 0.32682745121162426 1107 88-89 0.38848429704635024 1107 90-91 0.3628539059314413 1107 92-93 0.3869041193594214 1107 94-95 0.39634066577946925 1107 96-97 0.3566816527559702 1107 98-99 0.3783848185420311 1107 100 0.4714799404743708 1108 1 0.06496884029391481 1108 2 0.06211494757200242 1108 3 0.14424897868843445 1108 4 0.06301164813304894 1108 5 0.08205508049059063 1108 6 0.04933695306462482 1108 7 0.01988203525793608 1108 8 0.13447990857336123 1108 9 0.08879629126224131 1108 10-11 0.14125731947872566 1108 12-13 0.12777834257079945 1108 14-15 0.17595606936299646 1108 16-17 0.2112058580525229 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0.19161956099794253 2214 82-83 0.025218941371811354 2214 84-85 0.05446668413962641 2214 86-87 0.06486422930001723 2214 88-89 0.05615576414377088 2214 90-91 -0.026828943278477624 2214 92-93 0.014977929574584437 2214 94-95 0.013129390162461618 2214 96-97 -0.055256599107497806 2214 98-99 -0.13032053732995763 2214 100 -0.08496009521571679 2215 1 -0.1459309817855221 2215 2 -0.12498094141669469 2215 3 -0.10125818589892432 2215 4 -0.16563085997692895 2215 5 -0.06635238462705217 2215 6 -0.0724966883985445 2215 7 -0.005357919348107032 2215 8 -0.03161625630058751 2215 9 -0.07054061814330481 2215 10-11 -0.14064228023345038 2215 12-13 -0.12700623279471301 2215 14-15 -0.22151282397324223 2215 16-17 -0.09105128332140566 2215 18-19 -0.12294588548257934 2215 20-21 -0.16200701452319777 2215 22-23 -0.11811457825589855 2215 24-25 -0.09945755414955926 2215 26-27 -0.16795138150487077 2215 28-29 -0.07682615362799794 2215 30-31 -0.0739460035420123 2215 32-33 -0.02940969289763018 2215 34-35 -0.05236680702071084 2215 36-37 -0.24263967290082888 2215 38-39 -0.09641530148035571 2215 40-41 -0.0838043724978732 2215 42-43 -0.1072578848337642 2215 44-45 -0.08572520714527343 2215 46-47 0.006734113497522287 2215 48-49 -0.022111655500239635 2215 50-51 -0.030266141436570138 2215 52-53 -0.19716433052531102 2215 54-55 -0.10806012497998552 2215 56-57 -0.11214440977202855 2215 58-59 -0.07645092223529559 2215 60-61 -0.056089332092483346 2215 62-63 0.022831189042918254 2215 64-65 -5.397070844068708E-4 2215 66-67 -0.026839978021861555 2215 68-69 0.0793580922885937 2215 70-71 0.13933300165471962 2215 72-73 0.16579932099507033 2215 74-75 0.19622187110395828 2215 76-77 0.0777029951210153 2215 78-79 0.04635776719405982 2215 80-81 0.048172381366065053 2215 82-83 0.04122865697843281 2215 84-85 0.018205812485220463 2215 86-87 -0.12355405297092048 2215 88-89 0.03776622076603786 2215 90-91 0.0660006977562837 2215 92-93 -0.07084986505530111 2215 94-95 -0.2326519171862529 2215 96-97 -0.22809545683106336 2215 98-99 -0.23134947865293043 2215 100 -0.17955153979750094 2216 1 -0.057674180550463916 2216 2 -0.08511060264817161 2216 3 -0.09207464281671918 2216 4 -0.09606623082856203 2216 5 -0.0763275696454997 2216 6 -0.047815190726893775 2216 7 0.01802068441457294 2216 8 -1.4157689678029328E-4 2216 9 -0.08994248957517925 2216 10-11 -0.0858351023577626 2216 12-13 -0.15142915159383818 2216 14-15 -0.16476774744113953 2216 16-17 -0.20313200368603646 2216 18-19 -0.2242451151282694 2216 20-21 -0.24022349946656618 2216 22-23 -0.19061826869248932 2216 24-25 -0.25691135020552025 2216 26-27 -0.4135855057553428 2216 28-29 -0.30768705550922704 2216 30-31 -0.3882892923968342 2216 32-33 -0.3033131386204957 2216 34-35 -0.3287373891338987 2216 36-37 -0.3637917602289491 2216 38-39 -0.30309374345170426 2216 40-41 -0.3471142719950535 2216 42-43 -0.3413148497660998 2216 44-45 -0.32244151451280345 2216 46-47 -0.17403100749430678 2216 48-49 -0.14941507448607183 2216 50-51 -0.1473469034100532 2216 52-53 -0.07646108189417333 2216 54-55 -0.223242919371323 2216 56-57 -0.27759579600444084 2216 58-59 -0.2778902394850604 2216 60-61 -0.21132041019927073 2216 62-63 -0.17335266194478294 2216 64-65 -0.13812356569592765 2216 66-67 -0.19429164263089405 2216 68-69 -0.22312157373461616 2216 70-71 -0.26606944451618375 2216 72-73 -0.10650557309161712 2216 74-75 -0.08255403812324857 2216 76-77 -0.13758670046058086 2216 78-79 -0.15331048946758585 2216 80-81 -0.18905759397197386 2216 82-83 -0.1258504070855011 2216 84-85 -0.13181956220248026 2216 86-87 -0.15732379603492674 2216 88-89 -0.14429860635992853 2216 90-91 -0.14778191854917822 2216 92-93 -0.09937528229140469 2216 94-95 -0.11114115485573706 2216 96-97 -0.10888321437978021 2216 98-99 -0.16864557850772854 2216 100 -0.1074140072452856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40671.0 3 6868.0 4 4218.0 5 3452.0 6 2894.0 7 3013.0 8 3426.0 9 3750.0 10 4114.0 11 4517.0 12 4964.0 13 5389.0 14 5776.0 15 6012.0 16 5945.0 17 6012.0 18 6046.0 19 6191.0 20 6056.0 21 6397.0 22 7446.0 23 9834.0 24 14181.0 25 18338.0 26 18842.0 27 17527.0 28 19344.0 29 23494.0 30 28632.0 31 35280.0 32 45380.0 33 60623.0 34 84107.0 35 126030.0 36 232805.0 37 476883.0 38 730033.0 39 289146.0 40 534.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.38394198092118 19.477314428620083 13.067350576446696 24.07139301401204 2 15.488952366012807 19.94945725372324 40.53788850572096 24.023701874543 3 18.35694449771292 25.148682269015126 29.616799926833696 26.877573306438258 4 12.100842673075798 16.550041904013895 38.797976303690966 32.55113911921934 5 12.786228180249909 38.101815527934086 35.750258933740774 13.361697358075237 6 31.20643318702242 38.067305829127946 17.72829136372444 12.997969620125193 7 27.659419419925403 32.291633758142886 22.44780444860057 17.601142373331143 8 25.12247183958622 36.91237423703909 20.187832982491024 17.77732094088367 9 25.009373370192094 16.943303959227315 20.819203065534392 37.2281196050462 10-11 23.956048724466797 26.987425400455162 28.827315798209863 20.229210076868178 12-13 24.503258390732643 24.28548469549491 28.89479270425473 22.316464209517715 14-15 21.794521269312998 26.278591664999105 25.74177716458309 26.185109901104802 16-17 21.60714259384123 29.66806932710846 26.310868227645113 22.413919851405197 18-19 21.63574251456896 28.75870314456983 28.012508683660307 21.5930456572009 20-21 22.764014936795416 27.99161948625327 27.53233851596403 21.71202706098728 22-23 22.324837812789617 27.801794590951218 27.61018198668801 22.263185609571153 24-25 22.27810829131872 27.87705823147239 27.569547927412568 22.27528554979632 26-27 22.029383830151865 27.849424563675463 27.61180181070181 22.509389795470867 28-29 22.029344936442897 27.892792424079975 27.756740810828855 22.32112182864827 30-31 22.18684622274199 28.30369472883331 27.36568892693535 22.14377012148935 32-33 21.93789399756571 28.022187516193846 27.832769559320976 22.20714892691947 34-35 22.05310147574778 27.725809125274587 27.900654369014244 22.320435029963388 36-37 22.314778053807117 28.07787046232165 27.45628843158585 22.151063052285387 38-39 22.13441848807109 28.281740038881903 27.527608046826785 22.05623342622022 40-41 21.95710657348596 28.00433959637373 27.77337466803794 22.265179162102374 42-43 21.93177443093407 27.931219244660227 27.79452884567591 22.342477478729794 44-45 22.01529505427617 27.96456989768194 27.843064571161385 22.177070476880502 46-47 22.102008785276322 28.154312687765483 27.677938071721147 22.065740455237044 48-49 21.987381132100904 28.025171183253693 27.695167442832208 22.292280241813195 50-51 21.970168617655357 27.935644752970713 27.71405764929381 22.38012898008012 52-53 22.014407399193384 27.93524484217173 27.769068805514436 22.281278953120456 54-55 22.107155959902126 28.293923863912546 27.551850371363184 22.04706980482214 56-57 21.98901352785464 27.97367762077569 27.59827244117406 22.439036410195605 58-59 21.822517622990265 27.701818014864998 27.94877470435645 22.52688965778829 60-61 22.123533908853304 28.058473053829157 27.6215468275146 22.196446209802932 62-63 21.94093680091959 28.07527986383056 27.631490825369266 22.35229250988058 64-65 21.946698723362314 27.797396685181543 27.78360214091327 22.472302450542873 66-67 21.794060431017108 27.855737379000335 27.653684288782177 22.696517901200377 68-69 22.24592945662134 27.95954845194989 27.582142197494253 22.21237989393452 70-71 22.14606765709722 27.764341214572646 27.71095298929835 22.378638139031786 72-73 21.941491126074794 27.84300783638222 27.847641135014957 22.367859902528036 74-75 21.99727772415549 27.910214178621818 27.674800288865352 22.41770780835734 76-77 21.968000785143104 27.90073836727722 27.572717877737972 22.558542969841703 78-79 22.094624606137984 27.94407448928251 27.698337865953913 22.262963038625593 80-81 21.99017980385723 27.74498154448426 27.831562196330633 22.43327645532788 82-83 21.884327819909274 27.76203506612676 27.942354928542834 22.411282185421133 84-85 21.85405985880565 27.73512191022843 27.783076854063015 22.6277413769029 86-87 22.10424848659459 27.82713650049113 27.673395065784483 22.395219947129792 88-89 21.8338872064836 27.787582196456256 27.735316288192564 22.643214308867577 90-91 21.786071610336304 27.599151453153425 27.844913910040642 22.76986302646963 92-93 21.907734826295663 27.833988453611042 27.736737416266642 22.52153930382665 94-95 21.729658044786888 27.560681347059703 27.959041216179713 22.750619391973697 96-97 21.808418125617923 27.509073803659263 27.931045659350357 22.751462411372458 98-99 21.768681831864626 27.65203664203768 27.84669455442954 22.73258697166815 100 21.85737085856183 27.556952387892697 27.76625127475309 22.81942547879239 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 402.0 1 348.0 2 291.5 3 241.0 4 184.0 5 163.5 6 156.0 7 155.0 8 154.5 9 166.0 10 181.5 11 169.5 12 177.0 13 215.5 14 256.0 15 289.5 16 341.0 17 472.0 18 652.5 19 925.5 20 1342.5 21 1909.0 22 2861.0 23 4509.5 24 6715.5 25 9378.0 26 12675.0 27 17101.0 28 22509.5 29 28801.0 30 35369.0 31 42235.5 32 48860.5 33 55202.0 34 61726.0 35 67589.0 36 73017.5 37 77727.0 38 81195.0 39 83005.0 40 84399.5 41 86444.0 42 86926.0 43 86028.0 44 85097.0 45 84399.0 46 83470.5 47 82153.0 48 81167.0 49 79213.0 50 76139.0 51 72853.0 52 69111.5 53 65736.0 54 62786.5 55 59336.5 56 54860.5 57 49670.5 58 45102.0 59 41168.0 60 36640.0 61 31557.5 62 27249.5 63 23126.0 64 19242.5 65 15487.5 66 11813.0 67 8862.5 68 6535.5 69 4891.0 70 3553.0 71 2627.5 72 2092.5 73 1504.5 74 1120.5 75 822.5 76 504.5 77 328.5 78 225.5 79 144.0 80 81.5 81 53.5 82 37.0 83 28.0 84 29.5 85 23.0 86 11.5 87 5.0 88 6.0 89 6.5 90 6.0 91 4.5 92 2.5 93 2.0 94 2.5 95 2.5 96 1.0 97 1.0 98 3.0 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32752498767990496 2 0.11422939385132488 3 0.06288513459440562 4 0.03744466487235539 5 0.04110910339192223 6 0.00968759608621118 7 0.006949797192281934 8 0.01512107389108615 9 0.017858872785015394 10-11 0.02089151155982933 12-13 0.01581605361031434 14-15 0.01874339242766946 16-17 0.019648972061815286 18-19 0.019333072189438836 20-21 0.02394521032613503 22-23 0.015584393703904943 24-25 0.02489290994326438 26-27 0.018932932351095333 28-29 0.018469612538276534 30-31 0.01954367210435647 32-33 0.02455595007939617 34-35 0.015015773933627331 36-37 0.009582296128752364 38-39 0.013267794639810966 40-41 0.009118976315933568 42-43 0.00808703673283716 44-45 0.008423996596705374 46-47 0.008339756630738321 48-49 0.004464718196253849 50-51 0.0067391972773643005 52-53 0.007644776911510128 54-55 0.003327478655698623 56-57 0.002885218834371591 58-59 0.0029273388173551174 60-61 0.0035801985535997842 62-63 0.0029062788258633543 64-65 0.0018111592682916555 66-67 0.0017479792938163653 68-69 0.0029694588003386447 70-71 0.0021481191321598707 72-73 0.002169179123651634 74-75 0.002253419089618688 76-77 0.0033906586301739134 78-79 0.0035170185791244942 80-81 0.0034959585876327306 82-83 0.0023376590555857415 84-85 0.002800978868404537 86-87 0.002274479081110451 88-89 0.59486051967635 90-91 0.6053062754562647 92-93 0.6022315166984673 94-95 0.5893638618969999 96-97 0.58721574276484 98-99 0.5844358238879271 100 0.5858468433178753 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2374170.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.60398190828387 #Duplication Level Percentage of deduplicated Percentage of total 1 81.19075284508513 56.511996921492525 2 11.868871735747156 16.522414671333735 3 3.2489468051336803 6.784189039365042 4 1.3218425768384965 3.6802202721546418 5 0.6774379752789401 2.35761902876499 6 0.41640127568283475 1.7389912115528612 7 0.26555745926747487 1.2938699613324205 8 0.18861627820158536 1.0502755212440789 9 0.14083305088657963 0.8822287013398119 >10 0.6542767180238809 7.658097603101586 >50 0.021537285457702945 1.007079306052282 >100 0.004804887259518799 0.45885797434187137 >500 1.2110713705489263E-4 0.05415978792422105 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.211998298352687E-5 0.0 10-11 0.0 0.0 0.0 6.317997447529031E-5 0.0 12-13 0.0 0.0 0.0 1.0529995745881718E-4 0.0 14-15 0.0 0.0 0.0 1.2635994895058062E-4 0.0 16-17 0.0 0.0 0.0 1.2635994895058062E-4 0.0 18-19 0.0 0.0 0.0 1.4741994044234404E-4 0.0 20-21 0.0 0.0 0.0 1.684799319341075E-4 0.0 22-23 0.0 0.0 0.0 2.3165990640939781E-4 0.0 24-25 0.0 0.0 0.0 4.843798043105591E-4 0.0 26-27 0.0 0.0 0.0 5.686197702776128E-4 0.0 28-29 0.0 0.0 0.0 0.0011372395405552255 0.0 30-31 0.0 0.0 0.0 0.004169878315369161 0.0 32-33 0.0 0.0 0.0 0.011224975465109912 0.0 34-35 4.211998298352687E-5 0.0 0.0 0.020996811517288147 0.0 36-37 4.211998298352687E-5 0.0 0.0 0.040456243655677564 0.0 38-39 8.423996596705375E-5 0.0 0.0 0.07587914934482366 0.0 40-41 8.423996596705375E-5 0.0 0.0 0.11696719274525413 0.0 42-43 8.423996596705375E-5 0.0 0.0 0.16056137513320445 0.0 44-45 8.423996596705375E-5 0.0 0.0 0.2079884759726557 0.0 46-47 8.423996596705375E-5 0.0 0.0 0.2646187930940076 0.0 48-49 8.423996596705375E-5 0.0 0.0 0.3261350282414486 0.0 50-51 8.423996596705375E-5 0.0 0.0 0.3969387196367573 0.0 52-53 8.423996596705375E-5 0.0 0.0 0.4698273501897505 0.0 54-55 8.423996596705375E-5 0.0 0.0 0.5478124986837505 0.0 56-57 8.423996596705375E-5 0.0 0.0 0.6392971017239709 0.0 58-59 8.423996596705375E-5 0.0 0.0 0.7425121200251035 0.0 60-61 8.423996596705375E-5 0.0 0.0 0.8576049735275907 0.0 62-63 8.423996596705375E-5 0.0 0.0 0.9763622655496448 0.0 64-65 8.423996596705375E-5 0.0 0.0 1.0985102162018727 0.0 66-67 8.423996596705375E-5 0.0 0.0 1.2386223395965748 0.0 68-69 8.423996596705375E-5 0.0 0.0 1.3938134168993797 0.0 70-71 8.423996596705375E-5 0.0 0.0 1.5643572279996798 0.0 72-73 8.423996596705375E-5 0.0 0.0 1.741240096539001 0.0 74-75 1.684799319341075E-4 0.0 0.0 1.9218084635893806 0.0 76-77 1.684799319341075E-4 0.0 0.0 2.1150338855263104 0.0 78-79 1.684799319341075E-4 0.0 0.0 2.3346685367939113 0.0 80-81 2.1059991491763436E-4 0.0 0.0 2.5814073971114118 0.0 82-83 2.1059991491763436E-4 0.0 0.0 2.837854913506615 0.0 84-85 2.1059991491763436E-4 0.0 0.0 3.095229069527456 0.0 86-87 2.1059991491763436E-4 0.0 0.0 3.3635544211240136 0.0 88 2.1059991491763436E-4 0.0 0.0 3.5793982739230974 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2530 0.0 28.673794 1 GTACATG 6670 0.0 26.908167 1 TACCGTC 160 6.978371E-8 26.415611 7 TCAACGC 3560 0.0 25.204437 4 ATCAACG 3545 0.0 25.183344 3 GTATCAA 3785 0.0 25.015842 1 TACATGG 6845 0.0 24.583359 2 CAACGCA 3680 0.0 24.510212 5 CCGATCG 115 1.3855791E-4 24.501436 9 AACGCAG 3870 0.0 23.541216 6 ACCGTCC 240 2.6557245E-10 23.480541 8 ACATGGG 6915 0.0 23.17065 3 GTACCGC 300 9.094947E-12 21.915173 6 TATAGCG 135 4.0917803E-4 20.879068 5 TATCAAC 4435 0.0 20.878738 2 ACGCAGA 4365 0.0 20.656422 7 ATACCGT 320 2.1827873E-11 20.545475 6 TATACCG 165 6.887609E-5 19.930021 5 CGCAGAG 4585 0.0 19.358006 8 GAGTACA 4980 0.0 18.918451 1 >>END_MODULE