FastQCFastQC Report
Wed 25 May 2016
SRR1033148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374170
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38450.16195133457166083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.13352034605778018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39150.040.988931
GTATCAA65500.035.3482361
ATCAACG73750.030.8555723
TCAACGC74350.030.6065714
CAACGCA75600.030.163975
AACGCAG78000.029.283176
TATCAAC78650.028.8746662
GTACATG64050.027.9195481
TACATGG65300.026.4973962
ACGCAGA88300.025.7609047
CGCAGAG89700.025.1476738
ACATGGG65050.025.0080073
CATGGGG42300.022.8969254
GCAGAGT99150.022.466469
AGAGTAC82800.020.29059210-11
GAGTACT62950.020.26771512-13
CAGAGTA98350.018.82655510-11
TACGACC2001.4042227E-518.80664
TCTAGAC10400.018.5353493
GTACTTT68850.018.25788914-15
Produced by FastQC (version 0.11.5)