FastQCFastQC Report
Wed 25 May 2016
SRR1033147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4372514
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35300.036.1706661
GTATCAA58750.031.8379061
TCAACGC56700.031.734274
ATCAACG58100.031.1387923
CAACGCA58950.030.6023835
GTACATG122900.028.0621591
AACGCAG67200.027.1126236
TATCAAC71100.025.922822
TACATGG124700.025.6394232
ACATGGG129850.023.8846653
CGCAGAG76800.022.562348
ACGCAGA80600.022.0227137
CATGGGG82300.020.09354
GAGTACA88250.018.3122311
GCAGAGT95700.017.0760089
GTATTAG19950.016.2955151
AGTACAT93400.016.159352
CTGTCGC11200.015.9325269
GTATATA37450.015.7260661
AGAGTAC77450.015.58226910-11
Produced by FastQC (version 0.11.5)