##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033147_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4372514
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29634027472525	33.0	31.0	34.0	28.0	34.0
2	31.376580155032094	33.0	31.0	34.0	28.0	34.0
3	31.52905582463544	34.0	31.0	34.0	28.0	34.0
4	34.926750377471635	37.0	35.0	37.0	32.0	37.0
5	35.01486924913219	37.0	35.0	37.0	33.0	37.0
6	35.15583300590919	37.0	35.0	37.0	33.0	37.0
7	35.04062674241867	37.0	35.0	37.0	32.0	37.0
8	35.051397891464724	37.0	35.0	37.0	32.0	37.0
9	36.67993035585478	39.0	37.0	39.0	33.0	39.0
10-11	36.660990450802444	39.0	37.5	39.0	33.0	39.0
12-13	36.62125164150417	39.0	37.0	39.0	33.0	39.0
14-15	38.01869805333956	41.0	38.0	41.0	33.0	41.0
16-17	38.0135242562974	40.5	38.0	41.0	33.0	41.0
18-19	37.98986326859102	40.0	38.5	41.0	33.0	41.0
20-21	38.02839990906833	40.0	39.0	41.0	33.0	41.0
22-23	37.976859307940465	40.0	38.0	41.0	33.0	41.0
24-25	37.96932496957128	40.0	38.0	41.0	33.0	41.0
26-27	37.92994830891336	40.0	38.0	41.0	33.0	41.0
28-29	37.896712966499365	40.0	38.0	41.0	32.5	41.0
30-31	37.8922825633034	40.0	38.0	41.0	33.0	41.0
32-33	37.9660499657634	40.0	38.0	41.0	33.0	41.0
34-35	37.9364740741825	40.0	38.0	41.0	33.0	41.0
36-37	37.85019887872286	40.0	38.0	41.0	32.5	41.0
38-39	37.76725368975377	40.0	38.0	41.0	32.0	41.0
40-41	37.70339363121536	40.0	38.0	41.0	32.0	41.0
42-43	37.58152815519858	40.0	38.0	41.0	32.0	41.0
44-45	37.44000876841103	40.0	38.0	41.0	31.0	41.0
46-47	37.30820290112278	40.0	38.0	41.0	31.0	41.0
48-49	37.190300248323965	40.0	37.0	41.0	31.0	41.0
50-51	37.06281237750182	40.0	37.0	41.0	30.5	41.0
52-53	36.91140234199365	40.0	37.0	41.0	30.0	41.0
54-55	36.75926755180201	40.0	36.0	41.0	30.0	41.0
56-57	36.6043622730539	40.0	36.0	41.0	30.0	41.0
58-59	36.405705733589414	39.5	35.5	41.0	30.0	41.0
60-61	36.18614485854133	39.0	35.0	41.0	30.0	41.0
62-63	35.92954041999637	39.0	35.0	41.0	29.0	41.0
64-65	35.668536224240796	38.5	35.0	40.5	29.0	41.0
66-67	35.37745756788887	38.0	35.0	40.0	29.0	41.0
68-69	35.07297918314269	37.0	34.5	40.0	28.5	41.0
70-71	34.729575251217035	37.0	34.0	40.0	28.0	41.0
72-73	34.364512726545875	36.5	34.0	39.0	28.0	41.0
74-75	33.99073061858693	36.0	34.0	39.0	27.5	40.5
76-77	33.64843062823813	35.5	34.0	38.5	27.0	40.0
78-79	33.26998072504742	35.0	34.0	37.0	27.0	39.0
80-81	32.900903347593626	35.0	33.0	37.0	26.0	39.0
82-83	32.667745374857574	35.0	33.0	36.5	26.0	39.0
84-85	32.38453175907499	35.0	33.0	36.0	26.0	37.0
86-87	32.11842432065398	35.0	33.0	36.0	26.0	37.0
88-89	31.747028711629053	35.0	33.0	35.0	25.0	37.0
90-91	31.54367681841613	35.0	33.0	35.0	25.0	36.0
92-93	31.352725800306185	35.0	33.0	35.0	24.0	36.0
94-95	31.171251481413208	35.0	33.0	35.0	23.5	36.0
96-97	30.981165068882568	35.0	33.0	35.0	21.5	36.0
98-99	30.770492558743094	35.0	32.5	35.0	19.5	35.0
100	30.591104796920032	35.0	32.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2338499478205982
1101	2	0.2772532355000443
1101	3	0.2339214957552258
1101	4	0.18269758623820564
1101	5	0.16638360831578325
1101	6	0.2223966871709706
1101	7	0.18004385508484688
1101	8	0.18365264437336037
1101	9	0.27057985470401036
1101	10-11	0.26473475392533174
1101	12-13	0.2236490660295587
1101	14-15	0.3038121500166824
1101	16-17	0.2065733882862446
1101	18-19	0.23300048015888564
1101	20-21	0.25021142267613783
1101	22-23	0.23123128780633806
1101	24-25	0.2306730751587125
1101	26-27	0.2362121168165956
1101	28-29	0.3208057865976741
1101	30-31	0.33703525356909125
1101	32-33	0.31670477544108877
1101	34-35	0.3295372162539323
1101	36-37	0.23801640547853964
1101	38-39	0.2360172651172121
1101	40-41	0.23818817565785366
1101	42-43	0.20965117417636492
1101	44-45	0.24163999413759285
1101	46-47	0.2063488881129132
1101	48-49	0.1482333652210599
1101	50-51	0.15201819260963845
1101	52-53	0.18815000454186048
1101	54-55	0.1924561986955169
1101	56-57	0.1569055115711464
1101	58-59	0.14813720526363738
1101	60-61	0.1821205343890142
1101	62-63	0.22215444977751275
1101	64-65	0.2125210282052592
1101	66-67	0.12563314913969492
1101	68-69	0.15257660734643252
1101	70-71	0.21408926438121512
1101	72-73	0.314791059337189
1101	74-75	0.24506344571977934
1101	76-77	0.2673450680641096
1101	78-79	0.20936915471345685
1101	80-81	0.32216131396651804
1101	82-83	0.285414177102993
1101	84-85	0.21662027593065858
1101	86-87	0.2534454059690816
1101	88-89	0.3830284267574591
1101	90-91	0.4478925790656163
1101	92-93	0.4536503811302417
1101	94-95	0.4456022913053168
1101	96-97	0.4690080039772617
1101	98-99	0.5096752928772617
1101	100	0.45694099496368423
1102	1	0.2754889663307267
1102	2	0.2910726610053551
1102	3	0.2950912078350214
1102	4	0.2201068841790388
1102	5	0.1315368500874854
1102	6	0.20684321223613011
1102	7	0.15946187540764356
1102	8	0.2176401324938979
1102	9	0.2745963832312981
1102	10-11	0.21642352241500618
1102	12-13	0.2771846061093015
1102	14-15	0.3006983173964173
1102	16-17	0.27983861708175084
1102	18-19	0.30043096368607536
1102	20-21	0.2690055777067215
1102	22-23	0.30264411993913853
1102	24-25	0.3011532070204268
1102	26-27	0.36080905620443815
1102	28-29	0.33891339047899294
1102	30-31	0.3141755072091641
1102	32-33	0.31609102812149814
1102	34-35	0.3721875249946578
1102	36-37	0.3979743528412243
1102	38-39	0.3268850342742553
1102	40-41	0.432548005409636
1102	42-43	0.3847503198095552
1102	44-45	0.35772079427942316
1102	46-47	0.3621772686384048
1102	48-49	0.3078035524077336
1102	50-51	0.3196130857356465
1102	52-53	0.22065836102568426
1102	54-55	0.29731125077427123
1102	56-57	0.2904434531930278
1102	58-59	0.2435935245167542
1102	60-61	0.20354147306868242
1102	62-63	0.29840806551216303
1102	64-65	0.33089018305091855
1102	66-67	0.24074924634381034
1102	68-69	0.18286094253920027
1102	70-71	0.26350385716770575
1102	72-73	0.2950007057342674
1102	74-75	0.20763016814802882
1102	76-77	0.1278982053752955
1102	78-79	0.19845743840895125
1102	80-81	0.17498528534175506
1102	82-83	0.20288926155066633
1102	84-85	0.19490182877043338
1102	86-87	0.21472510223663477
1102	88-89	0.3291910833378857
1102	90-91	0.3440798952963391
1102	92-93	0.36029239888376097
1102	94-95	0.4107821075187452
1102	96-97	0.3940127223573704
1102	98-99	0.3662113051529232
1102	100	0.37469235219886343
1103	1	0.13551404985674154
1103	2	0.118308490582546
1103	3	0.09693669440013863
1103	4	0.13914156752976226
1103	5	0.10296414635902096
1103	6	0.14419143604413165
1103	7	0.1373345050025634
1103	8	0.10954320511842752
1103	9	0.15903335892189574
1103	10-11	0.17585105192963368
1103	12-13	0.18150595178745732
1103	14-15	0.1438807165532623
1103	16-17	0.17778888169338813
1103	18-19	0.17678280982838857
1103	20-21	0.1461387684485871
1103	22-23	0.15700907291911648
1103	24-25	0.1486141981577589
1103	26-27	0.18599958517785353
1103	28-29	0.19845810967091637
1103	30-31	0.1952720923515514
1103	32-33	0.15370809767237148
1103	34-35	0.20916016579383978
1103	36-37	0.2242069572644283
1103	38-39	0.1620123496190331
1103	40-41	0.22265028697486855
1103	42-43	0.13638604586887482
1103	44-45	0.06665059059363188
1103	46-47	0.1474388796619479
1103	48-49	0.18707520532119304
1103	50-51	0.10527550914163442
1103	52-53	0.10372834051317881
1103	54-55	0.14799456939282152
1103	56-57	0.1313315084893958
1103	58-59	0.09846929741010513
1103	60-61	0.09362768313287262
1103	62-63	0.18031888162671805
1103	64-65	0.15568486015619953
1103	66-67	0.13098743752151876
1103	68-69	0.11012175558417425
1103	70-71	0.1724516102610636
1103	72-73	0.18238120256354762
1103	74-75	0.17841265408985407
1103	76-77	0.11619978632936778
1103	78-79	0.12075095353849008
1103	80-81	0.13558247869124074
1103	82-83	0.15166790943939645
1103	84-85	0.1545560862936597
1103	86-87	0.2188713573055452
1103	88-89	0.23130485882461826
1103	90-91	0.24707480510203794
1103	92-93	0.21932344339339593
1103	94-95	0.21870518512018222
1103	96-97	0.3069264984998483
1103	98-99	0.38783386196536895
1103	100	0.43387241847246827
1104	1	0.11440893226107818
1104	2	0.09200201253108986
1104	3	0.10186726349526154
1104	4	0.10897757628174531
1104	5	0.10001360369313517
1104	6	0.11856446378038754
1104	7	0.1041268188605855
1104	8	0.051391663051887804
1104	9	0.09177239548233729
1104	10-11	0.0871228325814144
1104	12-13	0.11010801846711615
1104	14-15	0.04570982193081363
1104	16-17	0.1208248500071889
1104	18-19	0.07778654313862177
1104	20-21	0.07551024364770598
1104	22-23	0.07588070337195774
1104	24-25	0.04126528168425381
1104	26-27	0.042342963065387096
1104	28-29	0.17147429216262822
1104	30-31	0.14655383812539213
1104	32-33	0.10858162005418848
1104	34-35	0.09236099188802172
1104	36-37	0.11667426372081735
1104	38-39	0.018556113113476158
1104	40-41	0.03424521198371622
1104	42-43	-0.008449782355533841
1104	44-45	-0.04233390569832807
1104	46-47	-0.09813025156715582
1104	48-49	-0.14571401790476557
1104	50-51	-0.12321535255258453
1104	52-53	-0.1253257672112298
1104	54-55	-0.16490701419505172
1104	56-57	-0.1422522526942842
1104	58-59	-0.22179528380202385
1104	60-61	-0.14332306172389053
1104	62-63	-0.1442267304700806
1104	64-65	-0.17450584917273915
1104	66-67	-0.15190613064207525
1104	68-69	-0.12376688056611584
1104	70-71	-0.06570469773341614
1104	72-73	-0.08860404472312666
1104	74-75	-0.06053833628442362
1104	76-77	-0.134582981230551
1104	78-79	-0.11032686828801275
1104	80-81	-0.05745637806374049
1104	82-83	-0.09990917944516298
1104	84-85	-0.025086902376436626
1104	86-87	0.0058491812983803015
1104	88-89	0.0315720396338115
1104	90-91	0.006781772744275827
1104	92-93	-0.02028069574281588
1104	94-95	0.054887965678087625
1104	96-97	0.1029808878472771
1104	98-99	0.09502608050483019
1104	100	0.03774076332345899
1105	1	0.05487384831790365
1105	2	0.04404131584030324
1105	3	0.06427402176943176
1105	4	0.03530391491974427
1105	5	0.026553131351903403
1105	6	0.06218120640703262
1105	7	0.005677905646280124
1105	8	0.007116221014932478
1105	9	0.03965838490943696
1105	10-11	0.033977383460928934
1105	12-13	0.058660451350149856
1105	14-15	0.08218199916923652
1105	16-17	0.0819792366673866
1105	18-19	0.05933567113630289
1105	20-21	0.11617821313616616
1105	22-23	0.13566040805049084
1105	24-25	0.13681086108692142
1105	26-27	0.12235717566677806
1105	28-29	0.1424562143714212
1105	30-31	0.19005567278090751
1105	32-33	0.17294545809429707
1105	34-35	0.16410228610025257
1105	36-37	0.20013127401811914
1105	38-39	0.17106541166238287
1105	40-41	0.1308672137284077
1105	42-43	0.09720223212127621
1105	44-45	0.1344034367729776
1105	46-47	0.16470459299390683
1105	48-49	0.15474094477576728
1105	50-51	0.19541887111823542
1105	52-53	0.12276931144777592
1105	54-55	0.09023867635742988
1105	56-57	0.13518342047965604
1105	58-59	0.08426488743248939
1105	60-61	0.05303484528833735
1105	62-63	0.09892366117364304
1105	64-65	0.12192642345411286
1105	66-67	0.11950569998197835
1105	68-69	0.07765324074208024
1105	70-71	0.05104567295582285
1105	72-73	-9.001086980475748E-4
1105	74-75	0.029245559289407197
1105	76-77	0.030471898998669644
1105	78-79	0.008549129042570769
1105	80-81	-0.006928985779993013
1105	82-83	0.025067987008540626
1105	84-85	1.0174485267810951E-4
1105	86-87	0.17077355158220087
1105	88-89	0.15301780739899584
1105	90-91	0.1483953129802842
1105	92-93	0.16601462115947285
1105	94-95	0.1516090456535757
1105	96-97	0.15819023901013907
1105	98-99	0.222268949719286
1105	100	0.32057494736298153
1106	1	0.22754119852537613
1106	2	0.1945576553349717
1106	3	0.18120131192272737
1106	4	0.19635264559772736
1106	5	0.16305034892484116
1106	6	0.2072133342322644
1106	7	0.1464640329115241
1106	8	0.2015839148627805
1106	9	0.20624427793708833
1106	10-11	0.2632905365744804
1106	12-13	0.21573487570172745
1106	14-15	0.27886179116465826
1106	16-17	0.2860058878419238
1106	18-19	0.27581346010288854
1106	20-21	0.2720295364034797
1106	22-23	0.2661937928938656
1106	24-25	0.29862878387609726
1106	26-27	0.33139813700116605
1106	28-29	0.3164839200277427
1106	30-31	0.3259075479755822
1106	32-33	0.31809485259492476
1106	34-35	0.2757639650275081
1106	36-37	0.2675456472226756
1106	38-39	0.2601976256230998
1106	40-41	0.18198490598892647
1106	42-43	0.21302249528281436
1106	44-45	0.21600338069296043
1106	46-47	0.2613508066255221
1106	48-49	0.2689137262323129
1106	50-51	0.21531079591767366
1106	52-53	0.11339293856125465
1106	54-55	0.19052095595672824
1106	56-57	0.21258402960805967
1106	58-59	0.16463026954666304
1106	60-61	0.11841115396618562
1106	62-63	0.1374562408804607
1106	64-65	0.18872406434388722
1106	66-67	0.248023667447697
1106	68-69	0.1703642886176766
1106	70-71	0.18765975172597393
1106	72-73	0.2583356407772115
1106	74-75	0.2606930064428923
1106	76-77	0.24795253091590297
1106	78-79	0.2949203609666142
1106	80-81	0.24866850337507884
1106	82-83	0.1674928781978764
1106	84-85	0.18091385182058417
1106	86-87	0.18927909877417193
1106	88-89	0.22006833549020044
1106	90-91	0.2324301414571721
1106	92-93	0.2227471486931698
1106	94-95	0.22366037194051103
1106	96-97	0.23190104438593195
1106	98-99	0.22988540418609915
1106	100	0.2884759587853729
1107	1	0.15732436844840336
1107	2	0.13997824866179087
1107	3	0.1758013716724811
1107	4	0.10324065968663376
1107	5	0.12173643606941198
1107	6	0.0965653829234796
1107	7	0.10392438108510049
1107	8	0.05578371296969209
1107	9	0.0836143384997996
1107	10-11	0.07676117938532201
1107	12-13	0.13618110839845343
1107	14-15	0.09922487019063908
1107	16-17	0.08595426309170762
1107	18-19	0.14970152168030637
1107	20-21	0.049840813533279515
1107	22-23	0.13486564006591806
1107	24-25	0.08860343799106118
1107	26-27	0.14936090224450282
1107	28-29	0.17023722859610757
1107	30-31	0.17487537402361397
1107	32-33	0.18595581023688368
1107	34-35	0.17484363638162392
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2216	48-49	-0.41110434699686493
2216	50-51	-0.3874143954411551
2216	52-53	-0.40299694727065827
2216	54-55	-0.41277279179139725
2216	56-57	-0.4414992645561213
2216	58-59	-0.45827721889081374
2216	60-61	-0.4226051147505956
2216	62-63	-0.33297613511047786
2216	64-65	-0.3613160825536994
2216	66-67	-0.42707226432015233
2216	68-69	-0.4523242959116516
2216	70-71	-0.5407347492178474
2216	72-73	-0.4415088325482941
2216	74-75	-0.44851604148502133
2216	76-77	-0.6251468151195567
2216	78-79	-0.5822185241815063
2216	80-81	-0.4946442395320574
2216	82-83	-0.4995899532992354
2216	84-85	-0.5630283189920675
2216	86-87	-0.5961098352809664
2216	88-89	-0.5907811616997911
2216	90-91	-0.6040486688424451
2216	92-93	-0.5228135255720332
2216	94-95	-0.5070679890519081
2216	96-97	-0.5535164698901305
2216	98-99	-0.5140965295516438
2216	100	-0.5012127646074127
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60552.0
3	10047.0
4	6479.0
5	5192.0
6	4486.0
7	4974.0
8	5933.0
9	6476.0
10	7123.0
11	7724.0
12	8413.0
13	9402.0
14	10037.0
15	10236.0
16	10326.0
17	10666.0
18	10687.0
19	10681.0
20	10721.0
21	11446.0
22	12912.0
23	17242.0
24	25389.0
25	33631.0
26	34851.0
27	31725.0
28	34116.0
29	42869.0
30	51949.0
31	63531.0
32	82274.0
33	111715.0
34	153276.0
35	234190.0
36	429489.0
37	872034.0
38	1376860.0
39	542208.0
40	652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.5355037528769	20.079532077861582	13.425409420397935	23.95955474886358
2	15.000509465176052	20.649871488840425	40.77823381589417	23.571385230089355
3	17.69428039576734	26.01400848308258	30.619698931581972	25.672012189568104
4	11.733432598900833	17.06229135527014	40.00265857228263	31.201617473546406
5	12.306091761725558	38.67177775988051	36.372060373987786	12.650070104406144
6	29.918526323927225	39.28391635390604	18.272143257899486	12.525414064267249
7	26.4739584548395	33.21655018084692	23.18872898486485	17.120762379448724
8	24.68184572368218	36.99565196208385	20.82572691650253	17.496775397731447
9	25.04730937575413	16.766630334957014	21.23204525413707	36.95401503515179
10-11	23.32102630568543	27.227620231989434	29.72076836935677	19.730585092968365
12-13	23.975624487273283	24.7485130138947	29.47628650731447	21.799575991517543
14-15	21.322148466954978	26.774667892570474	26.36123987688656	25.54194376358798
16-17	21.104737779837496	30.084962585398916	27.05908402907892	21.751215605684667
18-19	21.105050780699138	29.1615794850985	28.708024961968377	21.02534477223399
20-21	22.378093882673706	28.355537143405503	28.102215035790266	21.164153938130525
22-23	21.92915821773595	28.193672937460185	28.207122605850376	21.67004623895349
24-25	21.83148668165552	28.298692625284787	28.05893348702685	21.810887206032845
26-27	21.509961408790726	28.36091324217625	28.13162139120955	21.997503957823476
28-29	21.58608950412086	28.290269242967387	28.291115591676093	21.832525661235664
30-31	21.80858595389639	28.757821658746362	27.912390559682503	21.521201827674748
32-33	21.50711428132542	28.35750168679594	28.434455496324052	21.700928535554592
34-35	21.659662789144402	28.252377018132314	28.32446297506052	21.763497217662763
36-37	21.988847237157515	28.426485164282017	27.864192508841	21.72047508971946
38-39	21.75199407860668	28.556255523858493	28.108708620238502	21.583041777296323
40-41	21.471228324178686	28.310112200215226	28.372222012559195	21.846437463046893
42-43	21.509691294582943	28.216764515270594	28.32273079658488	21.95081339356158
44-45	21.618402514112148	28.37083611611758	28.3091154863087	21.70164588346157
46-47	21.68541903439468	28.58607154954795	28.12815133209931	21.600358083958064
48-49	21.649726450637267	28.364339253813238	28.119515947649965	21.866418347899533
50-51	21.629552831693893	28.27888049651619	28.135177886585865	21.95638878520405
52-53	21.61753591189411	28.34627515913923	28.18045450184215	21.85573442712451
54-55	21.785058707345176	28.604078432592335	28.019577958478315	21.591284901584178
56-57	21.667381189230085	28.326472277649025	27.9748334060816	22.03131312703929
58-59	21.49162800920415	28.06638128413904	28.38947785152104	22.052512855135774
60-61	21.79014219123762	28.391762235008123	27.9990330145041	21.81906255925016
62-63	21.617042814130944	28.325076763174444	28.00806380694801	22.049816615746607
64-65	21.595526497099712	28.11645744769049	28.202485469977955	22.085530585231837
66-67	21.479453539814912	28.12769872693275	28.165823962499587	22.22702377075275
68-69	21.79309871637268	28.403453500671834	27.913868320992595	21.889579461962892
70-71	21.76947084957293	28.0812580783455	28.035059347644193	22.11421172443738
72-73	21.510280735486518	28.188496293418837	28.209868870264344	22.091354100830305
74-75	21.593144830818073	28.28857367741372	28.082645983329325	22.035635508438883
76-77	21.670239885169092	28.17721029141819	28.0013548802907	22.151194943122015
78-79	21.65510880083397	28.272870382885895	28.12216185037464	21.9498589659055
80-81	21.70689621851704	28.109718227893932	28.1238410825494	22.059544471039626
82-83	21.48456292368639	28.072998161312128	28.3582983898016	22.08414052519988
84-85	21.48271083242717	28.048396564202555	28.21615430418675	22.252738299183523
86-87	21.658167150991925	28.056349296016887	28.073033474018654	22.212450078972537
88-89	21.465389559724375	27.983227617207536	28.252482183341144	22.29890063972694
90-91	21.394627500108154	27.95278317530419	28.26229078896137	22.390298535626282
92-93	21.485068967727084	28.05013939434592	28.17701005197129	22.287781585955706
94-95	21.31801530391792	27.83436308911088	28.369020080812856	22.478601526158343
96-97	21.379807304058293	27.88884586510364	28.288829023707486	22.44251780713058
98-99	21.39979093862621	27.868741574535605	28.2072843406083	22.524183146229884
100	21.46153410787687	27.891067564191115	28.131814431651954	22.515583896280063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	440.0
1	422.5
2	371.0
3	307.5
4	245.5
5	213.0
6	206.0
7	208.0
8	222.0
9	242.0
10	241.5
11	238.0
12	261.0
13	294.5
14	347.5
15	437.5
16	555.0
17	746.0
18	1054.0
19	1423.0
20	2093.0
21	3184.0
22	5098.5
23	8711.5
24	12972.5
25	17709.5
26	25207.5
27	35989.5
28	50907.0
29	64982.0
30	75478.0
31	88275.5
32	102333.0
33	116442.5
34	130634.5
35	141509.0
36	149116.0
37	157699.0
38	164463.0
39	167023.5
40	169685.5
41	169816.5
42	165016.0
43	160634.5
44	157088.5
45	151007.5
46	145214.5
47	139374.0
48	134219.0
49	131246.0
50	128183.0
51	123063.0
52	114736.5
53	107937.0
54	104059.5
55	99377.0
56	91526.0
57	82504.0
58	74676.0
59	67541.5
60	60201.0
61	52183.0
62	44369.0
63	37232.5
64	31301.5
65	25457.5
66	20080.0
67	15118.0
68	11025.0
69	8523.0
70	6260.5
71	4479.0
72	3212.5
73	2338.0
74	1830.0
75	1308.0
76	777.5
77	534.5
78	415.5
79	271.0
80	154.0
81	103.0
82	79.0
83	53.5
84	46.0
85	35.5
86	26.0
87	22.0
88	17.5
89	12.5
90	9.0
91	8.5
92	6.0
93	4.0
94	6.5
95	6.5
96	2.5
97	2.5
98	3.5
99	3.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3321658890057299
2	0.11867314775893228
3	0.06577451781743866
4	0.039954131650579046
5	0.043224561430792445
6	0.00976554906399385
7	0.007044002603536547
8	0.015025680878323087
9	0.01735843498728649
10-11	0.020411598453429766
12-13	0.01526581733071638
14-15	0.017690051992972463
16-17	0.01886786411661575
18-19	0.018833558909130993
20-21	0.022973053945624874
22-23	0.01507142115496943
24-25	0.023864989340228526
26-27	0.01813601969027429
28-29	0.018147454759435876
30-31	0.01918804605314014
32-33	0.024253781691722428
34-35	0.014865589910060893
36-37	0.009456802196631045
38-39	0.013035978844207246
40-41	0.009582587957408484
42-43	0.008427645972088367
44-45	0.00901083449932922
46-47	0.008118899104725566
48-49	0.004048014483201198
50-51	0.006632340113719476
52-53	0.0073298793325761785
54-55	0.00338478047182925
56-57	0.002984553051173764
58-59	0.003133208950274373
60-61	0.0037278325466768092
62-63	0.0031446440194359583
64-65	0.0023213190398018164
66-67	0.0018753513424999897
68-69	0.0031789492269207143
70-71	0.0022755787631554753
72-73	0.002024007241600599
74-75	0.002412799593094499
76-77	0.003933663791585345
78-79	0.003819313099969491
80-81	0.003853618307454247
82-83	0.002344189178124987
84-85	0.0031446440194359583
86-87	0.0024699749389024256
88-89	0.604709327402954
90-91	0.6152524611699356
92-93	0.6117647650756521
94-95	0.5986487407473138
96-97	0.5964760776066126
98-99	0.5933314335871767
100	0.5955384019353626
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4372514.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.245828823739274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.32980138450927	36.22192935518913
2	15.534149418272525	16.2318902285891
3	6.264450726406534	9.818742609797546
4	3.085411757415154	6.447995781146587
5	1.724005182129084	4.503603981837778
6	1.058277010746886	3.317433573094817
7	0.7178360004446113	2.6252755761922746
8	0.4464945316692632	1.8661981497862363
9	0.3516630455234396	1.6535634552047207
>10	1.405656006278354	12.226626191431686
>50	0.05502729010496269	1.9719858854706063
>100	0.025475893280407044	2.4117077373115325
>500	0.001270021322357514	0.4042518299207193
>1k	4.8173189713739676E-4	0.29879564502724965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.861041496951182E-5	0.0
3	0.0	0.0	0.0	6.861041496951182E-5	0.0
4	0.0	0.0	0.0	6.861041496951182E-5	0.0
5	0.0	0.0	0.0	6.861041496951182E-5	0.0
6	0.0	0.0	0.0	6.861041496951182E-5	0.0
7	0.0	0.0	0.0	6.861041496951182E-5	0.0
8	0.0	0.0	0.0	6.861041496951182E-5	0.0
9	0.0	0.0	0.0	4.5740276646341214E-4	0.0
10-11	0.0	0.0	0.0	4.5740276646341214E-4	0.0
12-13	0.0	0.0	0.0	4.5740276646341214E-4	0.0
14-15	0.0	0.0	0.0	4.688378356249974E-4	0.0
16-17	0.0	0.0	0.0	4.8027290478658275E-4	0.0
18-19	0.0	0.0	0.0	4.8027290478658275E-4	0.0
20-21	0.0	0.0	0.0	5.603183889176799E-4	0.0
22-23	0.0	0.0	0.0	5.831885272408505E-4	0.0
24-25	0.0	0.0	0.0	6.289288038871917E-4	0.0
26-27	0.0	0.0	0.0	7.661496338262153E-4	0.0
28-29	0.0	0.0	0.0	0.001772435720045722	0.0
30-31	0.0	0.0	0.0	0.0056031838891767985	0.0
32-33	0.0	0.0	0.0	0.015414473229816988	0.0
34-35	0.0	0.0	0.0	0.029754049958444957	0.0
36-37	0.0	0.0	0.0	0.05515133856632591	0.0
38-39	0.0	0.0	0.0	0.09829585451298727	0.0
40-41	0.0	0.0	0.0	0.15038259454400832	0.0
42-43	0.0	0.0	0.0	0.20362427656034948	0.0
44-45	0.0	0.0	0.0	0.26315524661556255	0.0
46-47	0.0	0.0	0.0	0.33871818363531825	0.0
48-49	0.0	0.0	0.0	0.4245155075546928	0.0
50-51	0.0	0.0	0.0	0.5165792493746161	0.0
52-53	0.0	0.0	0.0	0.6050523794778015	0.0
54-55	0.0	0.0	0.0	0.7025020388728316	0.0
56-57	0.0	2.2870138323170605E-5	0.0	0.8215982841907424	0.0
58-59	0.0	2.2870138323170605E-5	0.0	0.9544051774333941	0.0
60-61	0.0	2.2870138323170605E-5	0.0	1.095045093051732	0.0
62-63	0.0	2.2870138323170605E-5	0.0	1.2343814107856486	0.0
64-65	0.0	2.2870138323170605E-5	0.0	1.38601042786827	0.0
66-67	0.0	2.2870138323170605E-5	0.0	1.5567931857965465	0.0
68-69	0.0	2.2870138323170605E-5	0.0	1.7511321861976885	0.0
70-71	0.0	2.2870138323170605E-5	0.0	1.96220069278223	0.0
72-73	0.0	2.2870138323170605E-5	0.0	2.18119370229575	0.0
74-75	0.0	4.574027664634121E-5	0.0	2.4039145443559473	0.0
76-77	0.0	4.574027664634121E-5	0.0	2.6444397891007325	0.0
78-79	0.0	4.574027664634121E-5	0.0	2.9164343441782004	0.0
80-81	0.0	4.574027664634121E-5	0.0	3.2152327013704243	0.0
82-83	0.0	4.574027664634121E-5	0.0	3.526529589156261	0.0
84-85	0.0	4.574027664634121E-5	0.0	3.8378036068037744	0.0
86-87	0.0	4.574027664634121E-5	0.0	4.167270819487371	0.0
88	0.0	4.574027664634121E-5	0.0	4.43589202916217	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3530	0.0	36.170666	1
GTATCAA	5875	0.0	31.837906	1
TCAACGC	5670	0.0	31.73427	4
ATCAACG	5810	0.0	31.138792	3
CAACGCA	5895	0.0	30.602383	5
GTACATG	12290	0.0	28.062159	1
AACGCAG	6720	0.0	27.112623	6
TATCAAC	7110	0.0	25.92282	2
TACATGG	12470	0.0	25.639423	2
ACATGGG	12985	0.0	23.884665	3
CGCAGAG	7680	0.0	22.56234	8
ACGCAGA	8060	0.0	22.022713	7
CATGGGG	8230	0.0	20.0935	4
GAGTACA	8825	0.0	18.312231	1
GCAGAGT	9570	0.0	17.076008	9
GTATTAG	1995	0.0	16.295515	1
AGTACAT	9340	0.0	16.15935	2
CTGTCGC	1120	0.0	15.932526	9
GTATATA	3745	0.0	15.726066	1
AGAGTAC	7745	0.0	15.582269	10-11
>>END_MODULE