FastQCFastQC Report
Wed 25 May 2016
SRR1033147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4372514
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64630.1478097039826516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53520.12240098030560909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55600.053.147421
GTATCAA111600.045.66271
TCAACGC131300.038.1664924
ATCAACG132200.037.942223
CAACGCA134850.037.161745
TATCAAC144650.034.7090382
AACGCAG144900.034.66936
CGCAGAG161000.030.9097798
ACGCAGA162950.030.713627
GTACATG122500.027.4258081
GCAGAGT186350.026.4023829
TACATGG125800.025.3360422
ACATGGG130400.023.5770743
AGAGTAC166900.021.77859110-11
GAGTACT116450.021.73061812-13
TACACCG4400.021.3681565
CAGAGTA178750.021.07091110-11
TGGTATC26000.020.9736672
GTGGTAT28500.020.4726541
CATGGGG80200.019.9294054
Produced by FastQC (version 0.11.5)