##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033147_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4372514 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75087604064847 34.0 31.0 34.0 31.0 34.0 2 32.82542583054051 34.0 31.0 34.0 31.0 34.0 3 32.92956889331858 34.0 31.0 34.0 31.0 34.0 4 36.364268244767196 37.0 37.0 37.0 35.0 37.0 5 36.306238927994286 37.0 37.0 37.0 35.0 37.0 6 36.38857531388121 37.0 37.0 37.0 35.0 37.0 7 36.354112302442026 37.0 37.0 37.0 35.0 37.0 8 36.34580152287677 37.0 37.0 37.0 35.0 37.0 9 38.16277935302208 39.0 39.0 39.0 37.0 39.0 10-11 38.14459450558649 39.0 39.0 39.0 37.0 39.0 12-13 38.120234377751565 39.0 39.0 39.0 37.0 39.0 14-15 39.65397903814602 41.0 40.0 41.0 37.0 41.0 16-17 39.666110045616776 41.0 40.0 41.0 37.0 41.0 18-19 39.679775639369026 41.0 40.0 41.0 37.0 41.0 20-21 39.674537119835406 41.0 40.0 41.0 37.0 41.0 22-23 39.608334816080635 41.0 40.0 41.0 37.0 41.0 24-25 39.56982321840479 41.0 40.0 41.0 37.0 41.0 26-27 39.476748387769604 41.0 39.5 41.0 36.5 41.0 28-29 39.384705800827625 41.0 39.0 41.0 36.0 41.0 30-31 39.2933403986814 41.0 39.0 41.0 36.0 41.0 32-33 39.33223347026447 41.0 39.0 41.0 36.0 41.0 34-35 39.283666330170696 41.0 39.0 41.0 35.5 41.0 36-37 39.20064795675897 41.0 39.0 41.0 35.0 41.0 38-39 39.112635659942995 41.0 39.0 41.0 35.0 41.0 40-41 39.003904504365224 41.0 39.0 41.0 35.0 41.0 42-43 38.90118373548947 41.0 39.0 41.0 35.0 41.0 44-45 38.77291221937769 41.0 39.0 41.0 35.0 41.0 46-47 38.58027212720188 41.0 39.0 41.0 35.0 41.0 48-49 38.4134855828935 41.0 39.0 41.0 35.0 41.0 50-51 38.291759614720505 41.0 38.0 41.0 34.5 41.0 52-53 38.16393772552815 40.5 38.0 41.0 34.0 41.0 54-55 38.03865785221042 40.0 38.0 41.0 34.0 41.0 56-57 37.88805787700166 40.0 37.5 41.0 34.0 41.0 58-59 37.70444085484918 40.0 37.0 41.0 34.0 41.0 60-61 37.520298734320804 40.0 36.5 41.0 33.0 41.0 62-63 37.29239231709721 39.5 36.0 41.0 33.0 41.0 64-65 37.050964502343504 39.0 35.5 41.0 33.0 41.0 66-67 36.77487550640204 39.0 35.0 41.0 33.0 41.0 68-69 36.45451655500703 38.5 35.0 41.0 32.0 41.0 70-71 36.12155661479872 37.0 35.0 40.0 32.0 41.0 72-73 35.76840394336073 37.0 35.0 39.5 32.0 41.0 74-75 35.42670452284429 36.5 35.0 39.0 31.0 41.0 76-77 35.074696387478696 36.0 35.0 39.0 31.0 40.5 78-79 34.72529762054507 35.5 35.0 37.5 31.0 39.5 80-81 34.377405423973485 35.0 35.0 37.0 31.0 39.0 82-83 34.04415183118911 35.0 34.5 37.0 31.0 39.0 84-85 33.76326010619977 35.0 34.0 36.0 30.0 38.0 86-87 33.52597990538166 35.0 34.0 36.0 30.0 37.0 88-89 33.30352401387394 35.0 34.0 36.0 30.0 37.0 90-91 33.100428494911625 35.0 34.0 35.0 30.0 36.0 92-93 32.938657600638905 35.0 34.0 35.0 30.0 36.0 94-95 32.79004321083934 35.0 34.0 35.0 30.0 36.0 96-97 32.66886029410083 35.0 34.0 35.0 29.5 36.0 98-99 32.55869060682253 35.0 34.0 35.0 29.5 36.0 100 32.460981714409606 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14291833907508078 1101 2 0.12672268704138645 1101 3 0.12691670529830645 1101 4 0.0776873144720227 1101 5 0.07759585367487887 1101 6 0.06687261531111943 1101 7 0.10155924913379977 1101 8 0.11636341013638685 1101 9 0.1356762270770986 1101 10-11 0.12621198035086678 1101 12-13 0.14956660508884312 1101 14-15 0.1950074467242615 1101 16-17 0.22691855196570998 1101 18-19 0.2240339193799059 1101 20-21 0.18724778583030144 1101 22-23 0.25022046691372424 1101 24-25 0.22552607688914605 1101 26-27 0.21857503907804698 1101 28-29 0.20143755478975578 1101 30-31 0.23716266465329738 1101 32-33 0.2012417338812611 1101 34-35 0.22278596928109806 1101 36-37 0.24070207661728915 1101 38-39 0.20896125264408028 1101 40-41 0.2760403393636395 1101 42-43 0.2566841576652905 1101 44-45 0.1859913161385407 1101 46-47 0.2513245317083772 1101 48-49 0.24106846771281454 1101 50-51 0.17253879152285378 1101 52-53 0.2677913301604562 1101 54-55 0.3079086900509225 1101 56-57 0.32611757667122276 1101 58-59 0.27779653629284695 1101 60-61 0.2568552000429918 1101 62-63 0.22968193777251855 1101 64-65 0.221677922588583 1101 66-67 0.285740193876066 1101 68-69 0.37049692883398677 1101 70-71 0.35498231706216643 1101 72-73 0.4028024785274056 1101 74-75 0.37879388309685424 1101 76-77 0.3505810684758188 1101 78-79 0.28491529412031724 1101 80-81 0.2504091924334517 1101 82-83 0.24586375567979246 1101 84-85 0.24688805769056188 1101 86-87 0.26233680153450223 1101 88-89 0.2593872969021689 1101 90-91 0.2621160071227493 1101 92-93 0.25927960748762047 1101 94-95 0.3315479107267123 1101 96-97 0.28964808699006994 1101 98-99 0.2652574776327512 1101 100 0.2756705808891624 1102 1 0.12877262356703056 1102 2 0.15487489303620094 1102 3 0.10997103980150769 1102 4 0.07835528438987183 1102 5 0.05042424756214814 1102 6 0.07502205112170657 1102 7 0.059448690866517495 1102 8 0.12325850843735964 1102 9 0.14997924180583766 1102 10-11 0.13546614940654678 1102 12-13 0.13516269099271483 1102 14-15 0.2005109211461047 1102 16-17 0.1619721971440029 1102 18-19 0.2097979223509867 1102 20-21 0.20711106256455025 1102 22-23 0.22960931798399287 1102 24-25 0.18751564777782903 1102 26-27 0.19454849279151176 1102 28-29 0.2017729117176117 1102 30-31 0.182384180371173 1102 32-33 0.16608938448587196 1102 34-35 0.24962197986267398 1102 36-37 0.21926819370899153 1102 38-39 0.21712121682494967 1102 40-41 0.2519746323117005 1102 42-43 0.24894016849978584 1102 44-45 0.26683205034949964 1102 46-47 0.2521336354738466 1102 48-49 0.2125414457706185 1102 50-51 0.23976735701361207 1102 52-53 0.3085774910263197 1102 54-55 0.30959868734754536 1102 56-57 0.2659309399826668 1102 58-59 0.29073160851925905 1102 60-61 0.20886169864226645 1102 62-63 0.20864956455498174 1102 64-65 0.20106814562778652 1102 66-67 0.1572437907014006 1102 68-69 0.1725473423692847 1102 70-71 0.19085680528792892 1102 72-73 0.1737373226490675 1102 74-75 0.165934155825191 1102 76-77 0.10475456814427986 1102 78-79 0.16472309081695613 1102 80-81 0.1617115202233066 1102 82-83 0.1054593481520456 1102 84-85 0.15124574717079753 1102 86-87 0.15546146555124096 1102 88-89 0.1980185896762876 1102 90-91 0.1985262909723886 1102 92-93 0.1884312510944781 1102 94-95 0.2105127816411425 1102 96-97 0.20745781255572382 1102 98-99 0.22216169654458184 1102 100 0.22139131434613546 1103 1 0.09897618498452943 1103 2 0.11244720433286659 1103 3 0.11059108995725353 1103 4 0.09017436688636593 1103 5 0.08019165794982541 1103 6 0.09586341879231242 1103 7 0.12065015965360715 1103 8 0.14410202425091967 1103 9 0.16590769349563317 1103 10-11 0.12212717097967385 1103 12-13 0.1468889369693187 1103 14-15 0.16900932343652642 1103 16-17 0.17121957095444884 1103 18-19 0.10991375530642244 1103 20-21 0.1320422145830875 1103 22-23 0.13005770325320753 1103 24-25 0.13365886345059153 1103 26-27 0.20433400467531726 1103 28-29 0.15839040173921148 1103 30-31 0.19158035206387325 1103 32-33 0.16850104238980634 1103 34-35 0.17131352032877345 1103 36-37 0.19081752428546395 1103 38-39 0.18611338107783837 1103 40-41 0.2077116663752463 1103 42-43 0.11430878587245275 1103 44-45 0.139869750032112 1103 46-47 0.11921593388278495 1103 48-49 0.07687422013454182 1103 50-51 0.05500510160371164 1103 52-53 0.0844040059843465 1103 54-55 0.05160045866099949 1103 56-57 0.08809589440981824 1103 58-59 0.12448952513264544 1103 60-61 0.1168764086729368 1103 62-63 -0.010286663186164446 1103 64-65 0.03262640626061142 1103 66-67 0.09226868485166051 1103 68-69 0.13582288839612744 1103 70-71 0.11743727884942956 1103 72-73 0.0796727857643802 1103 74-75 0.0795465589312272 1103 76-77 0.02695141684118596 1103 78-79 0.011178708657347158 1103 80-81 0.09353514434623378 1103 82-83 0.03065850926830649 1103 84-85 0.011371016126780376 1103 86-87 0.035618224021618516 1103 88-89 0.03835229689955355 1103 90-91 0.03134942622319414 1103 92-93 0.031174976849435154 1103 94-95 0.05500477070732046 1103 96-97 0.0786129193302969 1103 98-99 0.08980518316600694 1103 100 0.13633700759652356 1104 1 0.03632878422053665 1104 2 0.051372559791659 1104 3 0.09080165731931089 1104 4 0.03960281658777376 1104 5 -0.027330564994507256 1104 6 0.00720849363904108 1104 7 0.027567976280920448 1104 8 0.04396771173602332 1104 9 0.08994963366157549 1104 10-11 0.07497986770056997 1104 12-13 0.08447874943728095 1104 14-15 0.07701138867196278 1104 16-17 0.05342713433768154 1104 18-19 0.06350444874894379 1104 20-21 0.055681217204345046 1104 22-23 0.08492256031147605 1104 24-25 0.071965370386458 1104 26-27 0.009526338752422703 1104 28-29 0.08591435774825129 1104 30-31 0.05329442527141737 1104 32-33 0.10376818709348612 1104 34-35 0.09214748550861174 1104 36-37 0.05550596979863087 1104 38-39 0.017800663993426724 1104 40-41 0.022505539991506396 1104 42-43 0.006042067213847702 1104 44-45 0.054652069883637466 1104 46-47 0.04573976713307104 1104 48-49 0.03078155894147727 1104 50-51 0.05481374237550085 1104 52-53 0.09486377360925502 1104 54-55 0.07077970809955758 1104 56-57 0.11096368323588734 1104 58-59 0.13093688690268124 1104 60-61 0.11445589496366892 1104 62-63 0.1279006955510127 1104 64-65 0.11255607271786516 1104 66-67 0.09834452087485346 1104 68-69 0.11140581193186705 1104 70-71 0.10662259401447471 1104 72-73 0.17560405530648637 1104 74-75 0.1421708521657763 1104 76-77 0.10423346590136617 1104 78-79 0.08223111220146251 1104 80-81 0.0944088978033335 1104 82-83 0.10161234475714309 1104 84-85 0.11413814277769774 1104 86-87 0.15304274544302388 1104 88-89 0.0978151912620362 1104 90-91 0.11304790184836833 1104 92-93 0.10023471703414799 1104 94-95 0.02795596327246841 1104 96-97 0.06782386941080887 1104 98-99 0.09083924510862573 1104 100 0.0973639928582557 1105 1 0.0661260496613707 1105 2 0.08459724872017915 1105 3 0.08171948616224256 1105 4 0.057433937173954064 1105 5 0.06510373019484206 1105 6 0.046659395250941316 1105 7 0.006496655898359904 1105 8 -0.00652096031959104 1105 9 0.06518713670794085 1105 10-11 0.020772459382094155 1105 12-13 0.03688874198932979 1105 14-15 0.09621741900584624 1105 16-17 0.10104333488113326 1105 18-19 0.08239103720074326 1105 20-21 0.08969420442439713 1105 22-23 0.1292083657036045 1105 24-25 0.06837122215176095 1105 26-27 0.0904036707920497 1105 28-29 0.05379091766096877 1105 30-31 0.0610174025380843 1105 32-33 0.0032438696513921172 1105 34-35 0.019093461679730694 1105 36-37 0.0010873084708009628 1105 38-39 -0.03720472267360009 1105 40-41 0.02382908849542531 1105 42-43 0.024782429862952426 1105 44-45 0.07510045012539024 1105 46-47 0.1027935677512346 1105 48-49 0.030759807283907037 1105 50-51 0.010973333489893378 1105 52-53 0.011114197823523853 1105 54-55 0.018173722863352282 1105 56-57 0.05833560469304189 1105 58-59 0.023428693019106106 1105 60-61 -3.9900667556480585E-4 1105 62-63 -0.01157047723913962 1105 64-65 -0.031274997759780376 1105 66-67 -0.036846244578505605 1105 68-69 -0.06647343562021746 1105 70-71 0.014572881796873105 1105 72-73 -0.029759265704704774 1105 74-75 -0.0592725012793025 1105 76-77 -0.05722931598759118 1105 78-79 -0.03917604921861084 1105 80-81 -0.008930835755940336 1105 82-83 0.06750984548030203 1105 84-85 0.020568433839933675 1105 86-87 -0.03919794860567549 1105 88-89 -0.04066064389912327 1105 90-91 -0.06304520435666205 1105 92-93 0.04185964557255062 1105 94-95 0.02654426930804732 1105 96-97 -0.0036072074442827784 1105 98-99 -0.04773088519632296 1105 100 -0.03382720079527246 1106 1 0.02612996801170908 1106 2 0.03932921130351019 1106 3 0.028980107764589036 1106 4 0.02876689207688088 1106 5 -0.012924082126097858 1106 6 0.03483116735302616 1106 7 0.0498331383660684 1106 8 0.07595673653190005 1106 9 0.08767939308836503 1106 10-11 0.065800537195841 1106 12-13 0.07077947902392623 1106 14-15 0.11782294230561519 1106 16-17 0.09247729821545647 1106 18-19 0.1405832623378771 1106 20-21 0.09911807788734706 1106 22-23 0.1071200132957486 1106 24-25 0.06072206706215155 1106 26-27 0.04133853294031553 1106 28-29 0.024257675760580355 1106 30-31 0.11686894524065394 1106 32-33 0.11811410935840883 1106 34-35 0.1154871339589505 1106 36-37 0.0739157456193098 1106 38-39 0.08283753435438967 1106 40-41 0.06161691211441678 1106 42-43 0.08129357823511896 1106 44-45 0.11406466135691318 1106 46-47 0.24441376363678557 1106 48-49 0.23240502546658348 1106 50-51 0.21498114185479977 1106 52-53 0.21380279484921516 1106 54-55 0.2084845736479295 1106 56-57 0.21217201146751563 1106 58-59 0.1460057849758627 1106 60-61 0.1569063852039534 1106 62-63 0.15979037456271072 1106 64-65 0.13757429833128754 1106 66-67 0.13754389894336327 1106 68-69 0.1482695864997865 1106 70-71 0.1411553230126188 1106 72-73 0.16137657814440587 1106 74-75 0.1311749479492832 1106 76-77 0.1087017655536684 1106 78-79 0.14647492704047238 1106 80-81 0.10632941433298271 1106 82-83 0.12783469504132938 1106 84-85 0.18146806780523406 1106 86-87 0.17639049552373365 1106 88-89 0.17136802378997373 1106 90-91 0.1615511164726513 1106 92-93 0.1515427698592262 1106 94-95 0.14579604662028345 1106 96-97 0.1278223199587316 1106 98-99 0.1792113892839211 1106 100 0.2424704981375143 1107 1 -0.0028699001866954177 1107 2 0.011362246515048469 1107 3 0.01065119999868358 1107 4 0.011059282836470175 1107 5 -0.07418110361284391 1107 6 -0.08625145899788578 1107 7 -0.030320247926439947 1107 8 0.03321621716553835 1107 9 -0.006346445969249714 1107 10-11 -0.018675517194814972 1107 12-13 0.018420154426216584 1107 14-15 0.046670987951571874 1107 16-17 0.050154101413440344 1107 18-19 0.04813436846956165 1107 20-21 0.029842679686673534 1107 22-23 0.036768863119768014 1107 24-25 0.05455016061456064 1107 26-27 0.07531443895491208 1107 28-29 0.03662575476148788 1107 30-31 0.06481434802294928 1107 32-33 0.10995114567980124 1107 34-35 0.10478292249518972 1107 36-37 0.1346175282035844 1107 38-39 0.11078851459728156 1107 40-41 0.13446626865194844 1107 42-43 0.11821659655023353 1107 44-45 0.1391275552684732 1107 46-47 0.11478734663064927 1107 48-49 0.0814590478078756 1107 50-51 0.08988285505093785 1107 52-53 0.04641978079195752 1107 54-55 0.07308514912333663 1107 56-57 0.07084376130286074 1107 58-59 0.03664369903373199 1107 60-61 0.08517153380543618 1107 62-63 0.08701464449836749 1107 64-65 0.06808748210993087 1107 66-67 0.09381965502086587 1107 68-69 0.061694338122748604 1107 70-71 0.07092140796358848 1107 72-73 0.11338782990313234 1107 74-75 0.12388303423025349 1107 76-77 0.1204632146007043 1107 78-79 0.07337294888431956 1107 80-81 0.11007621904047937 1107 82-83 0.16381551004713657 1107 84-85 0.12380334887832589 1107 86-87 0.09482210824911874 1107 88-89 0.12747337258648628 1107 90-91 0.1348824734248879 1107 92-93 0.10288169506836198 1107 94-95 0.013746535868762066 1107 96-97 0.04995614175739149 1107 98-99 0.06138379044696052 1107 100 0.09408808309797934 1108 1 0.051896602983248385 1108 2 0.08034417621986734 1108 3 0.03451624917948237 1108 4 0.023375487969836684 1108 5 0.012588232182238812 1108 6 0.011821087138983444 1108 7 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-0.061221734418168694 2214 34-35 -0.10343261876714394 2214 36-37 -0.13365318023007688 2214 38-39 -0.17318443786123794 2214 40-41 -0.0919089505099393 2214 42-43 -0.15317721591847544 2214 44-45 -0.15005723047783448 2214 46-47 -0.12419030279699683 2214 48-49 -0.25213624788937494 2214 50-51 -0.15727087188302846 2214 52-53 -0.23777983077347642 2214 54-55 -0.19161007602935598 2214 56-57 -0.21537720614848155 2214 58-59 -0.2592396837086781 2214 60-61 -0.3615336549389241 2214 62-63 -0.3437117499744744 2214 64-65 -0.21856900188547002 2214 66-67 -0.35922335408432815 2214 68-69 -0.30857887327020705 2214 70-71 -0.2597231375486544 2214 72-73 -0.34559286371652576 2214 74-75 -0.27352625266176744 2214 76-77 -0.21420453731925448 2214 78-79 -0.43234384585175434 2214 80-81 -0.39638533212132643 2214 82-83 -0.33827313208485776 2214 84-85 -0.36713782762870295 2214 86-87 -0.3172234617797045 2214 88-89 -0.41925179971800475 2214 90-91 -0.26855632323411527 2214 92-93 -0.2869746114795291 2214 94-95 -0.30193097748403375 2214 96-97 -0.4551218644044823 2214 98-99 -0.5442541638978255 2214 100 -0.7242174852449601 2215 1 -0.06801352151841655 2215 2 -0.1228691284043606 2215 3 -0.11133712995427913 2215 4 -0.07706634445835903 2215 5 -0.05529705689104958 2215 6 -0.07769703480546752 2215 7 -0.08925002970395468 2215 8 -0.09822831541264065 2215 9 -0.10413153099282368 2215 10-11 -0.13834514830843858 2215 12-13 -0.09812792208729348 2215 14-15 -0.17062727224699614 2215 16-17 -0.1749992434389469 2215 18-19 -0.1266345740595085 2215 20-21 -0.14308557794331023 2215 22-23 -0.12070244940024821 2215 24-25 -0.08746718690861854 2215 26-27 -0.11076171709550664 2215 28-29 -0.08707026542461449 2215 30-31 -0.12086550043697031 2215 32-33 -0.11711171139753418 2215 34-35 -0.10167044032898787 2215 36-37 -0.0745383606403962 2215 38-39 -0.15416944189093584 2215 40-41 -0.1107003959773607 2215 42-43 -0.0563203432236179 2215 44-45 -0.09193138673746404 2215 46-47 -0.15427339991919808 2215 48-49 -0.10187268980458697 2215 50-51 -0.04733539043915869 2215 52-53 -0.025227614729637082 2215 54-55 -0.04013001253812121 2215 56-57 -0.20034777561625816 2215 58-59 -0.10673049209333385 2215 60-61 -0.06203711382227084 2215 62-63 -0.1218678996390068 2215 64-65 -0.026005745774725142 2215 66-67 -0.07337189435018843 2215 68-69 -0.06489453670877765 2215 70-71 0.05020214304702364 2215 72-73 0.09277538400926488 2215 74-75 9.988291258835602E-4 2215 76-77 -0.008076685241675818 2215 78-79 0.009113775773464283 2215 80-81 -0.017696387091604038 2215 82-83 0.036485773400301014 2215 84-85 -0.0066315327648638345 2215 86-87 -0.04424152957989236 2215 88-89 -0.04703315864460933 2215 90-91 -0.018675513995766835 2215 92-93 0.017576052009680154 2215 94-95 0.03916729936842955 2215 96-97 0.028596930766816797 2215 98-99 0.016552658082183314 2215 100 0.05602296538655338 2216 1 -0.23537178959271188 2216 2 -0.23600808478433066 2216 3 -0.30423928074061024 2216 4 -0.1632537754604897 2216 5 -0.1993838184929544 2216 6 -0.17068722203989495 2216 7 -0.2033952588464345 2216 8 -0.204897135965318 2216 9 -0.2493928625394659 2216 10-11 -0.2796514629564797 2216 12-13 -0.2773808137388727 2216 14-15 -0.44948381443070673 2216 16-17 -0.4342829425487551 2216 18-19 -0.4093743700576198 2216 20-21 -0.398526557624983 2216 22-23 -0.39597149970077083 2216 24-25 -0.4622706414980513 2216 26-27 -0.4844299108223211 2216 28-29 -0.4944926414125774 2216 30-31 -0.44880067350158015 2216 32-33 -0.47012614508314243 2216 34-35 -0.4081518953174097 2216 36-37 -0.4324008592594524 2216 38-39 -0.46311607638443064 2216 40-41 -0.5796967235089809 2216 42-43 -0.5346221272929625 2216 44-45 -0.444037571993384 2216 46-47 -0.45553827146891024 2216 48-49 -0.4996844091699373 2216 50-51 -0.48348488999845074 2216 52-53 -0.469264960145928 2216 54-55 -0.4413798029117615 2216 56-57 -0.43459493416902717 2216 58-59 -0.4229930534479607 2216 60-61 -0.3813987012124187 2216 62-63 -0.3505830086063 2216 64-65 -0.3690399446099377 2216 66-67 -0.3568969116170422 2216 68-69 -0.350659575562176 2216 70-71 -0.37294409760060176 2216 72-73 -0.3252339842027112 2216 74-75 -0.36148465576101785 2216 76-77 -0.3568753771731892 2216 78-79 -0.38135499380656057 2216 80-81 -0.34692266646148084 2216 82-83 -0.3775682136334595 2216 84-85 -0.35510161136713947 2216 86-87 -0.39706895535759656 2216 88-89 -0.3626716427857275 2216 90-91 -0.3687698160532449 2216 92-93 -0.35710805133127366 2216 94-95 -0.4084772090302664 2216 96-97 -0.39823893252155784 2216 98-99 -0.3873314700032964 2216 100 -0.36402381871326384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33.0 3 16.0 4 18.0 5 49.0 6 80.0 7 184.0 8 428.0 9 851.0 10 1584.0 11 2667.0 12 4205.0 13 6318.0 14 7518.0 15 9924.0 16 11863.0 17 17496.0 18 20128.0 19 10953.0 20 7695.0 21 7494.0 22 7990.0 23 8371.0 24 9746.0 25 11753.0 26 15051.0 27 19054.0 28 24353.0 29 31862.0 30 40789.0 31 52374.0 32 67881.0 33 88559.0 34 122209.0 35 187179.0 36 349264.0 37 811187.0 38 1578668.0 39 834034.0 40 2686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48800224332011 20.13535461053788 13.644193406241238 23.73244973990077 2 15.302427945073521 20.709106057615582 40.51064583050788 23.477820166803014 3 17.64726196416981 26.079847886135987 31.016115671670807 25.2567744780234 4 11.867339202585246 17.136355475865663 39.864791463932 31.131513857617087 5 12.048418802323752 38.38344089406579 36.556776875235656 13.011363428374803 6 29.77872877047424 39.38299012442976 17.965826012399752 12.87245509269625 7 26.20375562973948 33.35701262214351 23.252665177905715 17.186566570211305 8 24.80186383161296 36.4237330697464 20.81494376898514 17.9594593296555 9 25.32515099461247 15.945497628705912 21.48115486150165 37.24819651517996 10-11 23.46188608891302 26.696341542065888 29.779053072179796 20.0627192968413 12-13 24.489870129632518 24.263604416132228 29.753249503603644 21.493275950631606 14-15 21.53449532734326 26.141743448417813 27.124530804657503 25.19923041958142 16-17 20.70485080207862 29.644902223297624 27.76358177469529 21.886665199928462 18-19 20.63512755552735 28.24701844422222 30.253898935565005 20.863955064685424 20-21 21.88268579586023 27.461330026616267 29.82005317764563 20.835930999877874 22-23 21.402273180777847 27.348392198298114 29.918004714686024 21.33132990623801 24-25 21.316101741527724 27.454896345041057 29.783054887334202 21.445947026097016 26-27 21.013175795397977 27.578683298686467 29.876136934228743 21.53200397168681 28-29 21.07076430166591 27.55583754255345 29.939917404442852 21.433480751337786 30-31 21.355580653054304 27.988103739836173 29.517266655890985 21.139048951218538 32-33 21.101215762222473 27.565668682947898 30.04536975907567 21.28774579575396 34-35 21.26009560376197 27.476948068771296 29.887082053814318 21.375874273652414 36-37 21.578730347366427 27.699571703982194 29.378933600677588 21.342764347973787 38-39 21.38757645646448 27.784664648415784 29.53964618540061 21.288112709719126 40-41 21.19646114066388 27.622136572883882 29.678673905745185 21.50272838070705 42-43 21.272954195462827 27.551393882053247 29.490714010557394 21.68493791192653 44-45 21.40204946542199 27.730849654095234 29.391851319626156 21.475249560856614 46-47 21.518394509598686 28.016184460209963 29.025631723701284 21.43978930649007 48-49 21.54495100644331 27.891052135786502 28.837769520027713 21.726227337742472 50-51 21.567232265650606 27.925103249432375 28.676730755991464 21.830933728925555 52-53 21.515954855528268 28.004786525272664 28.684558653002988 21.79469996619608 54-55 21.674773307937325 28.220866121832678 28.565315468304046 21.539045101925947 56-57 21.54194404563624 27.919101683799695 28.56606865447543 21.97288561608864 58-59 21.355313579516753 27.6785337537363 28.990903657119322 21.97524900962762 60-61 21.579463694660973 27.975235166319973 28.670456802593396 21.77484433642566 62-63 21.432888218023596 27.872120115381204 28.663859324122278 22.03113234247292 64-65 21.43471796872114 27.687253059175916 28.81368801900385 22.064340953099094 66-67 21.36918962564021 27.719047595632833 28.743474973021677 22.168287805705283 68-69 21.71491170894254 27.990472204497685 28.45939480430969 21.835221282250078 70-71 21.719289522831115 27.712545551764688 28.48371800284414 22.084446922560055 72-73 21.5071389528621 27.845003647807086 28.561266006325916 22.086591393004902 74-75 21.598638852909506 27.918312420947476 28.42551787157359 22.05753085456943 76-77 21.674457409576267 27.826628583164265 28.383770188978975 22.11514381828049 78-79 21.668068416042264 27.88929705746982 28.44766024110851 21.994974285379403 80-81 21.66404097249293 27.79936029650809 28.450624694896696 22.085974036102275 82-83 21.50030960566587 27.77838066231027 28.59827821513906 22.1230315168848 84-85 21.476959873317384 27.72801476134259 28.44312020897875 22.35190515636128 86-87 21.60972851390192 27.78976365392169 28.270163058611107 22.330344773565283 88-89 21.410691881146633 27.67622928136994 28.439920375326416 22.473158462157013 90-91 21.362751964688496 27.667525250070756 28.408197803894215 22.561524981346537 92-93 21.358495675141505 27.743942670953754 28.316988422904632 22.580573231000116 94-95 21.187799513049015 27.460678227674055 28.57224699566428 22.77927526361265 96-97 21.29040638863592 27.53923715281415 28.44542064359314 22.724935814956794 98-99 21.25080674412935 27.558276542968187 28.34245928086222 22.848457432040238 100 21.308542408326193 27.536218294555486 28.280549816421402 22.874689480696915 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 55.0 1 44.0 2 121.5 3 214.5 4 159.5 5 132.0 6 293.0 7 652.0 8 1197.5 9 1480.5 10 1284.5 11 1148.0 12 1225.5 13 1402.0 14 1678.0 15 1934.5 16 2217.0 17 2518.0 18 2857.0 19 3343.0 20 3963.5 21 4973.5 22 6805.0 23 10249.0 24 14404.5 25 18876.5 26 25853.5 27 35993.0 28 50743.5 29 64634.5 30 74733.5 31 87129.0 32 101362.0 33 115775.0 34 129257.0 35 140306.5 36 148595.5 37 157109.0 38 163144.5 39 165357.0 40 168565.0 41 168462.0 42 163872.5 43 160401.0 44 156136.0 45 150210.5 46 145938.5 47 140159.0 48 134292.5 49 131127.0 50 127741.5 51 122477.5 52 114727.5 53 108189.0 54 104312.5 55 98988.0 56 90701.5 57 82252.0 58 74875.0 59 67574.5 60 60022.0 61 51976.5 62 43903.5 63 36769.0 64 30658.5 65 24395.0 66 19023.5 67 14201.0 68 10398.5 69 8011.0 70 5632.5 71 3838.0 72 2810.5 73 2136.0 74 1631.0 75 1155.0 76 663.0 77 431.5 78 305.0 79 171.5 80 98.5 81 44.0 82 12.5 83 8.5 84 6.0 85 3.5 86 2.5 87 1.0 88 1.0 89 1.5 90 0.5 91 0.0 92 0.5 93 1.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09122898177112755 2 0.001166377054481701 3 0.0 4 2.0583124490853547E-4 5 0.0377357282332315 6 1.6009096826219424E-4 7 0.00452828738798778 8 3.659222131707297E-4 9 1.6009096826219424E-4 10-11 3.659222131707297E-4 12-13 0.0 14-15 3.430520748475591E-5 16-17 0.0 18-19 1.4865589910060894E-4 20-21 0.0 22-23 1.4865589910060894E-4 24-25 5.717534580792652E-5 26-27 0.001475123921844504 28-29 0.008370470626280442 30-31 0.03568885085330773 32-33 0.025157152155487666 34-35 0.02841614686653948 36-37 0.025648860129435836 38-39 0.024516788282438893 40-41 0.030451589177301664 42-43 0.022458475833353534 44-45 0.021383579332164518 46-47 0.015231512123231624 48-49 0.010508828559496893 50-51 0.007661496338262153 52-53 0.005488833197560946 54-55 0.003956533929908515 56-57 0.0025500204230335228 58-59 9.719808787347508E-4 60-61 5.374482505945093E-4 62-63 0.0011778121236432862 64-65 0.0030989037427896168 66-67 5.145781122713386E-4 68-69 0.0010062860862195067 70-71 3.659222131707297E-4 72-73 5.488833197560946E-4 74-75 5.831885272408505E-4 76-77 3.887923514939003E-4 78-79 3.430520748475591E-5 80-81 4.459676973018268E-4 82-83 3.77357282332315E-4 84-85 2.744416598780473E-4 86-87 1.4865589910060894E-4 88-89 0.0 90-91 3.430520748475591E-5 92-93 2.2870138323170605E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4372514.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.228842601803244 #Duplication Level Percentage of deduplicated Percentage of total 1 66.20930880270933 32.594076418227644 2 17.108741063356067 16.844870418459273 3 6.778569939677707 10.011034578771264 4 3.432580332045186 6.759278275371919 5 1.9109424899398437 4.703674352917328 6 1.1552273202657886 3.412230235120047 7 0.770491405853133 2.6551280101350194 8 0.5589063743382525 2.201145114515388 9 0.38122973309906555 1.6890748673275224 >10 1.5974911838569175 13.074682212866199 >50 0.06345628716193295 2.1142326301984737 >100 0.03068535126536342 2.768613695460788 >500 0.0017192062997305694 0.5292174284998672 >1k 5.575801128808593E-4 0.3724832504542226 >5k 9.293001881339573E-5 0.27025851167487513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6463 0.1478097039826516 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5352 0.12240098030560909 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.2870138323170605E-5 0.0 5 0.0 0.0 0.0 2.2870138323170605E-5 0.0 6 0.0 0.0 0.0 2.2870138323170605E-5 0.0 7 0.0 0.0 0.0 2.2870138323170605E-5 0.0 8 0.0 0.0 0.0 2.2870138323170605E-5 0.0 9 0.0 0.0 0.0 2.0583124490853544E-4 0.0 10-11 0.0 0.0 0.0 2.0583124490853544E-4 0.0 12-13 0.0 0.0 0.0 2.0583124490853544E-4 0.0 14-15 0.0 0.0 0.0 2.2870138323170607E-4 0.0 16-17 0.0 0.0 0.0 2.515715215548767E-4 0.0 18-19 0.0 0.0 0.0 2.6300659071646195E-4 0.0 20-21 0.0 0.0 0.0 3.0874686736280315E-4 0.0 22-23 0.0 0.0 0.0 3.316170056859738E-4 0.0 24-25 0.0 0.0 0.0 3.77357282332315E-4 0.0 26-27 0.0 0.0 0.0 5.145781122713386E-4 0.0 28-29 0.0 0.0 0.0 0.0015208641984908453 0.0 30-31 0.0 0.0 0.0 0.005363047436783507 0.0 32-33 0.0 0.0 0.0 0.015174336777423698 0.0 34-35 0.0 0.0 0.0 0.02945673816024374 0.0 36-37 0.0 0.0 0.0 0.054911202113932626 0.0 38-39 0.0 0.0 0.0 0.09820437395969459 0.0 40-41 0.0 0.0 0.0 0.1507828219646638 0.0 42-43 0.0 0.0 0.0 0.20436755605585255 0.0 44-45 0.0 0.0 0.0 0.2644702795691449 0.0 46-47 0.0 0.0 0.0 0.34087941170685787 0.0 48-49 0.0 0.0 0.0 0.42733996963760434 0.0 50-51 0.0 0.0 0.0 0.5200898156072228 0.0 52-53 0.0 0.0 0.0 0.6092947901367497 0.0 54-55 0.0 0.0 0.0 0.7078422161712918 0.0 56-57 0.0 0.0 0.0 0.8279904878520686 0.0 58-59 0.0 0.0 0.0 0.9625469466764429 0.0 60-61 0.0 0.0 0.0 1.1047077264932712 0.0 62-63 0.0 0.0 0.0 1.244935979621792 0.0 64-65 0.0 0.0 0.0 1.3982802570786508 0.0 66-67 0.0 0.0 0.0 1.5707897104503268 0.0 68-69 0.0 0.0 0.0 1.7670154972631305 0.0 70-71 0.0 0.0 0.0 1.9801651864350807 0.0 72-73 0.0 0.0 0.0 2.20117076812104 0.0 74-75 0.0 0.0 0.0 2.426555981295886 0.0 76-77 0.0 0.0 0.0 2.670614662411601 0.0 78-79 0.0 0.0 0.0 2.9472289854303497 0.0 80-81 0.0 0.0 0.0 3.2510587730536713 0.0 82-83 0.0 0.0 0.0 3.567787318691261 0.0 84-85 0.0 0.0 0.0 3.8849503969569907 0.0 86-87 0.0 0.0 0.0 4.22043245601958 0.0 88 0.0 0.0 0.0 4.493913570088055 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5560 0.0 53.14742 1 GTATCAA 11160 0.0 45.6627 1 TCAACGC 13130 0.0 38.166492 4 ATCAACG 13220 0.0 37.94222 3 CAACGCA 13485 0.0 37.16174 5 TATCAAC 14465 0.0 34.709038 2 AACGCAG 14490 0.0 34.6693 6 CGCAGAG 16100 0.0 30.909779 8 ACGCAGA 16295 0.0 30.71362 7 GTACATG 12250 0.0 27.425808 1 GCAGAGT 18635 0.0 26.402382 9 TACATGG 12580 0.0 25.336042 2 ACATGGG 13040 0.0 23.577074 3 AGAGTAC 16690 0.0 21.778591 10-11 GAGTACT 11645 0.0 21.730618 12-13 TACACCG 440 0.0 21.368156 5 CAGAGTA 17875 0.0 21.070911 10-11 TGGTATC 2600 0.0 20.973667 2 GTGGTAT 2850 0.0 20.472654 1 CATGGGG 8020 0.0 19.929405 4 >>END_MODULE