FastQCFastQC Report
Wed 25 May 2016
SRR1033146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306110
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC38300.032.138374
CAACGCA39200.031.519875
ATCAACG40450.030.4356773
GTATCAA42250.030.3338361
AACGCAG41950.029.4411336
GGTATCA28650.028.4515931
TAGACCG1101.02182596E-425.6255575
ACGCAGA47850.025.6147047
GTACATG77350.025.3406721
CGCAGAG47800.025.2485268
TATCAAC51600.024.0553052
TACATGG79050.023.5532042
ACATGGG82100.021.2911933
GCAGAGT61550.018.7688589
GAGTACA58350.018.7340761
CTGTCGC7450.018.2798449
CATGGGG52050.017.0593474
GTACTAG7100.016.590781
ATTATAC20450.016.5439573
TACCGCG2003.0137997E-416.4361028
Produced by FastQC (version 0.11.5)