##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033146_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3306110
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.309527208713565	33.0	31.0	34.0	30.0	34.0
2	31.50182238340527	33.0	31.0	34.0	30.0	34.0
3	31.73363378713957	34.0	31.0	34.0	30.0	34.0
4	35.11142460474697	37.0	35.0	37.0	33.0	37.0
5	35.253636146407715	37.0	35.0	37.0	33.0	37.0
6	35.31808409278578	37.0	36.0	37.0	33.0	37.0
7	35.22436549298118	37.0	35.0	37.0	33.0	37.0
8	35.26392588268388	37.0	35.0	37.0	33.0	37.0
9	36.92044154610706	39.0	38.0	39.0	34.0	39.0
10-11	36.90872898965854	39.0	38.0	39.0	34.0	39.0
12-13	36.870034269882126	39.0	38.0	39.0	34.0	39.0
14-15	38.30548756695936	41.0	39.0	41.0	34.0	41.0
16-17	38.316613028604614	41.0	39.0	41.0	34.0	41.0
18-19	38.29226175172635	41.0	39.0	41.0	34.0	41.0
20-21	38.309932670116844	41.0	39.0	41.0	34.0	41.0
22-23	38.27184001742229	40.5	39.0	41.0	34.0	41.0
24-25	38.26359997096285	40.5	39.0	41.0	34.0	41.0
26-27	38.21318029345666	40.0	39.0	41.0	33.5	41.0
28-29	38.18944590470372	40.0	38.5	41.0	33.5	41.0
30-31	38.1841798972206	40.0	38.5	41.0	33.0	41.0
32-33	38.25274643009458	41.0	39.0	41.0	34.0	41.0
34-35	38.23450535523621	41.0	39.0	41.0	33.5	41.0
36-37	38.15791489091409	41.0	38.5	41.0	33.0	41.0
38-39	38.08484684417639	41.0	38.0	41.0	33.0	41.0
40-41	38.02263627042052	40.5	38.0	41.0	33.0	41.0
42-43	37.91840788721488	40.0	38.0	41.0	33.0	41.0
44-45	37.79848553133441	40.0	38.0	41.0	33.0	41.0
46-47	37.67682956707429	40.0	38.0	41.0	32.5	41.0
48-49	37.565747509913464	40.0	38.0	41.0	32.0	41.0
50-51	37.45047548327189	40.0	38.0	41.0	32.0	41.0
52-53	37.30639603642952	40.0	37.0	41.0	31.5	41.0
54-55	37.16418177253631	40.0	37.0	41.0	31.0	41.0
56-57	37.016970548469345	40.0	37.0	41.0	31.0	41.0
58-59	36.82525415064835	40.0	36.0	41.0	31.0	41.0
60-61	36.616177471409	39.5	36.0	41.0	31.0	41.0
62-63	36.3636802768208	39.0	35.0	41.0	30.0	41.0
64-65	36.10125525164014	39.0	35.0	41.0	30.0	41.0
66-67	35.81702695917559	38.5	35.0	40.5	30.0	41.0
68-69	35.514015413885204	38.0	35.0	40.0	29.5	41.0
70-71	35.16600899546597	37.0	35.0	40.0	29.0	41.0
72-73	34.797749772391114	37.0	34.0	39.5	29.0	41.0
74-75	34.41935522411535	36.0	34.0	39.0	29.0	41.0
76-77	34.07554754681483	36.0	34.0	39.0	28.5	40.0
78-79	33.69206408740181	35.0	34.0	37.5	28.0	39.5
80-81	33.31683776401874	35.0	34.0	37.0	28.0	39.0
82-83	33.073494983530495	35.0	34.0	37.0	28.0	39.0
84-85	32.78367522556721	35.0	34.0	36.0	27.0	37.5
86-87	32.50995081833332	35.0	34.0	36.0	27.0	37.0
88-89	32.129210008136454	35.0	33.5	35.5	26.0	37.0
90-91	31.923345109509363	35.0	33.0	35.0	26.0	36.0
92-93	31.738801340548257	35.0	33.0	35.0	25.5	36.0
94-95	31.56316592611861	35.0	33.0	35.0	25.0	36.0
96-97	31.379176887641364	35.0	33.0	35.0	25.0	36.0
98-99	31.177950370677323	35.0	33.0	35.0	24.0	35.5
100	31.0073013299618	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13537519803946196
1101	2	0.16526940398657075
1101	3	0.23757138833384772
1101	4	0.1552201218706557
1101	5	0.15399840998385628
1101	6	0.1571209562795488
1101	7	0.17107001674791178
1101	8	0.18664264657033414
1101	9	0.21137632960410713
1101	10-11	0.23209878063326528
1101	12-13	0.23519498503748792
1101	14-15	0.2853280359214523
1101	16-17	0.26288063272923523
1101	18-19	0.2944992809135556
1101	20-21	0.273610996222196
1101	22-23	0.30626888391632434
1101	24-25	0.30510131572534505
1101	26-27	0.2413626950528922
1101	28-29	0.2730483078142072
1101	30-31	0.3217913992469903
1101	32-33	0.3190165037024997
1101	34-35	0.32184332278202277
1101	36-37	0.31809854969610996
1101	38-39	0.28370016166169876
1101	40-41	0.22415759338488783
1101	42-43	0.2480714370849526
1101	44-45	0.17441903926264501
1101	46-47	0.24186433285014175
1101	48-49	0.11211615031782429
1101	50-51	0.12052954543293737
1101	52-53	0.1611043648791295
1101	54-55	0.13020046950944675
1101	56-57	0.1697695815296143
1101	58-59	0.18518386643339113
1101	60-61	0.152362295973127
1101	62-63	0.05445476018920914
1101	64-65	0.0697555798292484
1101	66-67	0.10277673381592933
1101	68-69	0.08832973356453522
1101	70-71	0.06814520872215013
1101	72-73	0.17364367264676872
1101	74-75	0.11285188729835482
1101	76-77	-0.008658722008824782
1101	78-79	0.14439302727556225
1101	80-81	0.1554767492456719
1101	82-83	0.15977045818794267
1101	84-85	0.12244143669717289
1101	86-87	0.18812185751792043
1101	88-89	0.26919598820273194
1101	90-91	0.2664586614329387
1101	92-93	0.2953218569613476
1101	94-95	0.3143239100521811
1101	96-97	0.31496183106501974
1101	98-99	0.41896764568213385
1101	100	0.4215765110786336
1102	1	0.1409606920693669
1102	2	0.179683894610978
1102	3	0.22280370715428788
1102	4	0.184028338488055
1102	5	0.09162898618655646
1102	6	0.11379006195119246
1102	7	0.11987436241601301
1102	8	0.12509181921246437
1102	9	0.23432105549802174
1102	10-11	0.2302126759313694
1102	12-13	0.28920350361962477
1102	14-15	0.32924923563183484
1102	16-17	0.2868666593311744
1102	18-19	0.3270690848426838
1102	20-21	0.2998361502377378
1102	22-23	0.2688644216018332
1102	24-25	0.29977346137738436
1102	26-27	0.3517704702132747
1102	28-29	0.33969749958090034
1102	30-31	0.35171258572172803
1102	32-33	0.35186583889381495
1102	34-35	0.3549175442066499
1102	36-37	0.33978382636396276
1102	38-39	0.27978188050897046
1102	40-41	0.33783364000623806
1102	42-43	0.4209830786388906
1102	44-45	0.34956585192050227
1102	46-47	0.3455775045224243
1102	48-49	0.36142892733743537
1102	50-51	0.3198830748400212
1102	52-53	0.3312263525329442
1102	54-55	0.3584555364871278
1102	56-57	0.2951032423015292
1102	58-59	0.31233100365250266
1102	60-61	0.3218798074629703
1102	62-63	0.3725316282651292
1102	64-65	0.3116924049007963
1102	66-67	0.24216582013666255
1102	68-69	0.23570404352417995
1102	70-71	0.27126495312440824
1102	72-73	0.2579511453340899
1102	74-75	0.32306109887249335
1102	76-77	0.3225677685200594
1102	78-79	0.2766048882823924
1102	80-81	0.297109350887105
1102	82-83	0.3159217332753812
1102	84-85	0.2979736898284884
1102	86-87	0.36178905831037156
1102	88-89	0.4932644882760542
1102	90-91	0.48798107241669086
1102	92-93	0.5387801348703825
1102	94-95	0.5056034026624161
1102	96-97	0.46201323422179996
1102	98-99	0.495439587102279
1102	100	0.541135518262621
1103	1	0.08906391181985285
1103	2	0.14080556852120552
1103	3	0.14963451157908025
1103	4	0.1179524598356636
1103	5	0.10315170147636366
1103	6	0.14114553320592194
1103	7	0.08570336228832787
1103	8	0.12470769769275591
1103	9	0.1478620367940806
1103	10-11	0.15428627581006538
1103	12-13	0.16809812394145496
1103	14-15	0.1784546766866555
1103	16-17	0.1811878892084664
1103	18-19	0.16933494130970672
1103	20-21	0.17170669133137295
1103	22-23	0.23136980281499575
1103	24-25	0.26005023539853056
1103	26-27	0.29677113421602286
1103	28-29	0.277638872440896
1103	30-31	0.30004728776124523
1103	32-33	0.2691618662037456
1103	34-35	0.33524487546680604
1103	36-37	0.34117723206080797
1103	38-39	0.313823312884999
1103	40-41	0.31747975793916794
1103	42-43	0.2898776082563188
1103	44-45	0.28069902203242236
1103	46-47	0.2929635315323367
1103	48-49	0.3211432177696736
1103	50-51	0.28342976586198176
1103	52-53	0.20972110598093963
1103	54-55	0.27192462872909573
1103	56-57	0.2001746882994908
1103	58-59	0.20957705516190117
1103	60-61	0.1844058878861503
1103	62-63	0.25984629184132757
1103	64-65	0.28249977409864613
1103	66-67	0.2791603785701824
1103	68-69	0.22062027335611134
1103	70-71	0.23594523987126337
1103	72-73	0.2596389217063333
1103	74-75	0.2755378523804808
1103	76-77	0.2731196815573256
1103	78-79	0.26093516766823655
1103	80-81	0.25573260689144917
1103	82-83	0.3058693131074648
1103	84-85	0.35746566144219116
1103	86-87	0.38416290418405197
1103	88-89	0.36312878751127187
1103	90-91	0.3611637341071692
1103	92-93	0.3988120302598297
1103	94-95	0.38862849219813
1103	96-97	0.4398020515782477
1103	98-99	0.5364736545133262
1103	100	0.56831097616287
1104	1	0.005396460340460152
1104	2	0.003149285077480357
1104	3	0.09599272524319957
1104	4	0.04505821580537628
1104	5	0.0385483264543538
1104	6	0.025723472179038254
1104	7	0.0417199435026987
1104	8	0.005556107088779072
1104	9	0.08097596293266207
1104	10-11	0.06325938861036917
1104	12-13	0.04582999552017242
1104	14-15	-0.0043121056400750035
1104	16-17	0.021352824247045987
1104	18-19	0.04404930940454932
1104	20-21	0.03951379103924779
1104	22-23	0.03116185035148078
1104	24-25	-0.021234042884678672
1104	26-27	0.07096486841258098
1104	28-29	0.04521412838867178
1104	30-31	0.10299180824328857
1104	32-33	0.11561878692326388
1104	34-35	0.10111907109921248
1104	36-37	0.13019509031406784
1104	38-39	0.09705011721789703
1104	40-41	0.057539243994838785
1104	42-43	0.07936262317692666
1104	44-45	0.08979787505381864
1104	46-47	0.04976152178337401
1104	48-49	-0.02936101949904213
1104	50-51	-0.0162775166633935
1104	52-53	-0.06694508285979595
1104	54-55	-0.04256868268970493
1104	56-57	-0.03678050013737533
1104	58-59	-0.052754224829037355
1104	60-61	-0.02920161280277256
1104	62-63	-0.07148671814130125
1104	64-65	-0.1083104550417886
1104	66-67	-0.042169967369247274
1104	68-69	-0.04606963005105058
1104	70-71	-0.07750202549166119
1104	72-73	0.024201845163204894
1104	74-75	-0.03281343215307686
1104	76-77	-0.01506433771803728
1104	78-79	-0.023206378772847813
1104	80-81	0.0014031439481598795
1104	82-83	0.09015322549382887
1104	84-85	0.055661966734994905
1104	86-87	0.06569309918285171
1104	88-89	0.15144273616658666
1104	90-91	0.1760289642850843
1104	92-93	0.1429359822337446
1104	94-95	0.11968835593264515
1104	96-97	0.1742277080816237
1104	98-99	0.21775446364936002
1104	100	0.1579145311877994
1105	1	0.05637346823027656
1105	2	0.0937948536921418
1105	3	0.07292698511801632
1105	4	0.1227534856773076
1105	5	0.21347597175955713
1105	6	0.1547159837503571
1105	7	0.12570662237830987
1105	8	0.109115556682994
1105	9	0.12678946719115913
1105	10-11	0.11663984343519473
1105	12-13	0.152497289358422
1105	14-15	0.22483293405989002
1105	16-17	0.24136534677295884
1105	18-19	0.16682130326663724
1105	20-21	0.18303061387162955
1105	22-23	0.22220634190347255
1105	24-25	0.1849657990680882
1105	26-27	0.22609977301979
1105	28-29	0.24071835343456627
1105	30-31	0.2820538731429991
1105	32-33	0.22814971693509278
1105	34-35	0.16808506585606153
1105	36-37	0.23191259969028266
1105	38-39	0.1542930918509029
1105	40-41	0.13853168030556162
1105	42-43	0.1985677041029632
1105	44-45	0.17328883751132196
1105	46-47	0.20725457063168307
1105	48-49	0.15777500147357415
1105	50-51	0.19401995081775425
1105	52-53	0.2437088480426226
1105	54-55	0.17066384326474804
1105	56-57	0.16700533749207835
1105	58-59	0.1444739276489173
1105	60-61	0.16414888412617046
1105	62-63	0.16974044758669748
1105	64-65	0.2051493578938306
1105	66-67	0.27011851901485784
1105	68-69	0.1170205906715367
1105	70-71	0.18074126690827796
1105	72-73	0.18213506393318823
1105	74-75	0.19579148738080931
1105	76-77	0.15613222203678134
1105	78-79	0.09031748928698136
1105	80-81	0.1013938618087522
1105	82-83	0.1287033093257648
1105	84-85	0.11578606308527384
1105	86-87	0.18557778323471297
1105	88-89	0.2055008289796234
1105	90-91	0.23448304905359763
1105	92-93	0.22948697755504455
1105	94-95	0.2745859806248063
1105	96-97	0.3372641801996643
1105	98-99	0.3191398647673971
1105	100	0.32653487914259216
1106	1	0.10199248360894231
1106	2	0.12010365209302165
1106	3	0.13088246114729074
1106	4	0.2325150680353687
1106	5	0.0850750948211072
1106	6	0.14444161807991662
1106	7	0.0933314732665309
1106	8	0.1525665674190435
1106	9	0.21921668452358745
1106	10-11	0.1317069840355174
1106	12-13	0.1922096245294611
1106	14-15	0.19531017234857018
1106	16-17	0.2018761150613173
1106	18-19	0.21266624313831528
1106	20-21	0.17853469228024466
1106	22-23	0.14609695027952796
1106	24-25	0.1976993806523737
1106	26-27	0.2487416307107324
1106	28-29	0.2213713018177117
1106	30-31	0.20612905771920254
1106	32-33	0.21382474998148382
1106	34-35	0.1771189827403461
1106	36-37	0.16295565672353263
1106	38-39	0.17344879678690717
1106	40-41	0.1569501282534631
1106	42-43	0.22632046871591882
1106	44-45	0.20876817142498538
1106	46-47	0.2275699621911471
1106	48-49	0.1900398078700576
1106	50-51	0.2319892902122831
1106	52-53	0.19447068832375436
1106	54-55	0.2752381895909508
1106	56-57	0.2724587178963773
1106	58-59	0.2551517817289408
1106	60-61	0.21644246506566134
1106	62-63	0.33300317447839944
1106	64-65	0.294579025495473
1106	66-67	0.302472147670116
1106	68-69	0.2144691672585708
1106	70-71	0.2340496945447299
1106	72-73	0.3090891536259406
1106	74-75	0.41012258000743884
1106	76-77	0.35336664187106237
1106	78-79	0.30490279734384984
1106	80-81	0.2545541448793216
1106	82-83	0.22656359562037665
1106	84-85	0.27063429147616347
1106	86-87	0.17234995655902452
1106	88-89	0.34676255641940656
1106	90-91	0.39397897064498366
1106	92-93	0.3760341935978566
1106	94-95	0.37550547638520015
1106	96-97	0.39646002441412875
1106	98-99	0.38094758311540033
1106	100	0.4282112179245523
1107	1	0.03839083331584803
1107	2	0.04532588470920018
1107	3	0.10243953432374653
1107	4	0.026120413843926826
1107	5	-0.03155515697100242
1107	6	-0.011829983473745642
1107	7	-0.08919357025688868
1107	8	-0.02318043147970883
1107	9	-0.0070409021658903725
1107	10-11	-0.011628673273811785
1107	12-13	-0.04103156404340069
1107	14-15	-0.027250876208405828
1107	16-17	-0.08186883061893013
1107	18-19	0.00908365006581846
1107	20-21	-0.1171738865331804
1107	22-23	-0.10119520476170862
1107	24-25	-0.10050029434602692
1107	26-27	-0.06417801251303246
1107	28-29	-0.02102960156138778
1107	30-31	-0.055541955886283745
1107	32-33	-0.06141476001847934
1107	34-35	-0.05850389530980493
1107	36-37	-0.14771622858521027
1107	38-39	-0.08798042220588087
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2216	46-47	-0.360677220148915
2216	48-49	-0.3847970903906486
2216	50-51	-0.3622687427886149
2216	52-53	-0.3839061562999362
2216	54-55	-0.3590245190616912
2216	56-57	-0.463188758102703
2216	58-59	-0.4571587987043273
2216	60-61	-0.428180024812157
2216	62-63	-0.4108274189925112
2216	64-65	-0.3470075529940644
2216	66-67	-0.33558515767937536
2216	68-69	-0.3482522755228814
2216	70-71	-0.35714414834599495
2216	72-73	-0.28796827781650336
2216	74-75	-0.2680876027720416
2216	76-77	-0.3834018239393018
2216	78-79	-0.4300434372707471
2216	80-81	-0.44850085420299735
2216	82-83	-0.4339128676891235
2216	84-85	-0.4728099916052244
2216	86-87	-0.49980815422519953
2216	88-89	-0.4883381704377072
2216	90-91	-0.4928059340562605
2216	92-93	-0.4554780255048989
2216	94-95	-0.42707074886533647
2216	96-97	-0.4142455621422343
2216	98-99	-0.5039808545204956
2216	100	-0.5305685468929333
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	38595.0
3	6381.0
4	4168.0
5	3336.0
6	2922.0
7	3198.0
8	3777.0
9	4158.0
10	4785.0
11	5199.0
12	5792.0
13	6209.0
14	6872.0
15	7049.0
16	7046.0
17	7187.0
18	7386.0
19	7243.0
20	7619.0
21	7748.0
22	8883.0
23	11890.0
24	17807.0
25	23265.0
26	23405.0
27	21314.0
28	23631.0
29	29331.0
30	35337.0
31	43991.0
32	57315.0
33	77321.0
34	107736.0
35	161724.0
36	298625.0
37	645033.0
38	1093092.0
39	478796.0
40	944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.401784719838616	20.206835457559038	13.550250479274725	23.84112934332762
2	15.080791305380112	20.33310438728465	41.21337771737155	23.372726589963683
3	17.79230366668049	25.698484154616775	30.375899750380846	26.133312428321894
4	11.837047912595477	16.981498617434546	40.30969587191192	30.871757598058053
5	12.316108995714165	38.48021867653249	36.59781632127512	12.605856006478227
6	30.171552716644594	39.28004244653195	18.065700165103458	12.482704671719997
7	26.616449423954663	32.82301386338317	23.239187598668202	17.321349113993968
8	24.223118272250975	37.22479813916849	20.915749818717767	17.63633376986277
9	24.54878506256202	17.067178537718725	21.58580193131489	36.79823446840437
10-11	23.22655185694003	27.245416681919433	29.840615887101485	19.68741557403905
12-13	23.9425567167868	24.636350958119138	29.59761204577336	21.8234802793207
14-15	21.296330050278193	26.762614587135385	26.46886250632845	25.47219285625797
16-17	20.96102506435922	29.99889123197439	27.043003409045703	21.99708029462069
18-19	20.841030143258987	29.153342840771785	28.93789771053057	21.06772930543866
20-21	22.16693736781668	28.47576419184248	28.304418456932346	21.05287998340849
22-23	21.701632110629333	28.248558429838845	28.354802475921897	21.695006983609918
24-25	21.681433466201753	28.319005226494014	28.259524547881792	21.740036759422438
26-27	21.39396002087446	28.29310462187734	28.35424560766607	21.958689749582135
28-29	21.414408859916247	28.348410252748906	28.425448662136432	21.811732225198412
30-31	21.70828033397424	28.868665810811155	27.99922673500199	21.423827120212604
32-33	21.285875779030206	28.454398322215624	28.604822873729148	21.654903025025025
34-35	21.44319692500045	28.190359679171255	28.639397100670628	21.727046295157663
36-37	21.844683982888267	28.550968872303905	28.096086819476785	21.508260325331037
38-39	21.595430908524392	28.767382906443135	28.245933526737232	21.391252658295244
40-41	21.325318151980905	28.406502491068753	28.523538860370074	21.744640496580264
42-43	21.313845031824677	28.32949140258025	28.49699766761705	21.859665897978022
44-45	21.493103613605737	28.45269661087677	28.45417884277316	21.600020932744332
46-47	21.52554877666974	28.59963827792706	28.33249088813084	21.542322057272358
48-49	21.395676433027	28.48475214868598	28.35509293176534	21.764478486521675
50-51	21.46093348256148	28.358329048065578	28.268671163677062	21.912066305695877
52-53	21.453759251514914	28.335305830297703	28.402564825829145	21.80837009235823
54-55	21.657286016441283	28.718839869540524	28.20199288719097	21.42188122682722
56-57	21.46127906306719	28.41451575556904	28.242783660057867	21.881421521305896
58-59	21.31275970674642	28.01532057123196	28.667269104396915	22.00465061762471
60-61	21.61678313978786	28.560100629630902	28.105500797115052	21.71761543346619
62-63	21.408322597837696	28.497727321536438	28.231681624773785	21.86226845585208
64-65	21.406186975871783	28.112320972720873	28.436683262122447	22.044808789284897
66-67	21.294000001814855	28.283456607293715	28.26337222263056	22.159171168260865
68-69	21.754546385358402	28.335600167634322	28.18687083248954	21.72298261451773
70-71	21.658791934818666	28.149339458690513	28.26966473620921	21.922203870281606
72-73	21.409053099620394	28.25625746737043	28.431148954190043	21.903540478819135
74-75	21.524092700238125	28.293672249138506	28.24721172583754	21.935023324785828
76-77	21.44828993263905	28.30545292635758	28.13240368899474	22.11385345200863
78-79	21.60063605479061	28.39563588010845	28.203606498418253	21.800121566682684
80-81	21.56028697018864	28.104321612782623	28.331848149479065	22.00354326754967
82-83	21.35030110104769	28.191823856567677	28.48056870575702	21.977306336627613
84-85	21.420289969050287	28.14689196154395	28.329105676695278	22.103712392710484
86-87	21.533432959475675	28.243043423880604	28.209362508134767	22.01416110850896
88-89	21.348015594183618	28.137924717377484	28.316053969192023	22.198005719246876
90-91	21.36145683855564	27.939249698318037	28.443884111518088	22.255409351608236
92-93	21.44819241101441	28.184154376307312	28.242556471843887	22.125096740834387
94-95	21.19499537547084	27.94613849913299	28.491557552679996	22.367308572716176
96-97	21.188739861865155	27.907964266783186	28.53148200102606	22.371813870325592
98-99	21.275759946373732	27.984125114979726	28.40664802648695	22.3334669121596
100	21.496663967655685	27.875823586313125	28.25560212135569	22.371910324675504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	257.0
1	243.0
2	207.0
3	167.5
4	129.5
5	106.5
6	102.5
7	109.5
8	132.0
9	145.0
10	135.5
11	142.5
12	173.5
13	194.5
14	222.0
15	280.0
16	352.0
17	504.0
18	771.0
19	1117.5
20	1668.5
21	2485.0
22	3846.5
23	6166.0
24	9230.5
25	13009.5
26	18372.0
27	25310.5
28	33990.5
29	44324.0
30	55135.0
31	66555.0
32	78593.0
33	89628.5
34	100020.0
35	109260.5
36	115978.0
37	122686.0
38	127919.0
39	130436.5
40	132167.0
41	131540.0
42	128042.5
43	124831.5
44	121803.5
45	117096.0
46	113822.0
47	110663.5
48	106496.0
49	103512.5
50	99809.0
51	94154.0
52	86689.5
53	80803.0
54	77075.0
55	73002.5
56	66921.5
57	60136.0
58	54327.0
59	48914.5
60	43272.0
61	36608.5
62	30447.0
63	25104.5
64	20353.5
65	15767.5
66	11979.5
67	8834.0
68	6250.5
69	4615.0
70	3122.0
71	2212.0
72	1700.5
73	1252.0
74	921.0
75	622.5
76	382.0
77	258.5
78	180.0
79	123.5
80	88.5
81	56.0
82	36.5
83	25.0
84	19.0
85	13.0
86	9.5
87	12.5
88	12.5
89	5.5
90	4.5
91	4.5
92	5.0
93	5.0
94	2.0
95	2.0
96	2.5
97	1.5
98	3.0
99	4.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3335339719489067
2	0.12165354449791446
3	0.06887248155687499
4	0.04186188602315107
5	0.0454612822924827
6	0.009195096351906016
7	0.006261134686988636
8	0.014851290489427153
9	0.01784574620929128
10-11	0.021142672203889164
12-13	0.015698207258681655
14-15	0.018193587025235096
16-17	0.019342974069223348
18-19	0.018995133253279534
20-21	0.02433373360233023
22-23	0.015516725093841404
24-25	0.025256267940268168
26-27	0.019206862445593158
28-29	0.018632168923599035
30-31	0.019524456234063597
32-33	0.02415225143748998
34-35	0.014624437783376838
36-37	0.009316084461799518
38-39	0.012703751538817523
40-41	0.009074108242012515
42-43	0.007970091739234327
44-45	0.008605279316175203
46-47	0.007773486060657389
48-49	0.0041135957363790075
50-51	0.006351875769408761
52-53	0.0070324338875597005
54-55	0.0031003203160209426
56-57	0.002676861931393692
58-59	0.002949085178654068
60-61	0.003796001947908569
62-63	0.0029944557198641306
64-65	0.002555873821500192
66-67	0.0017845746209291281
68-69	0.003160814370967693
70-71	0.0021626624643463163
72-73	0.0018148216484025032
74-75	0.002344144629186567
76-77	0.0034632846457014433
78-79	0.003191061398441068
80-81	0.0036901373517517566
82-83	0.0023290211154498794
84-85	0.0030851968022842556
86-87	0.002525626794026817
88-89	0.605333760824655
90-91	0.615980714495283
92-93	0.6130769998578389
94-95	0.5997834312832907
96-97	0.5975602747639976
98-99	0.594686807154027
100	0.5965016288024294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3306110.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.52399399358364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.70817931001685	45.34821581147462
2	15.045007488156056	18.51257900669468
3	5.288130211279922	9.760406740682223
4	2.2928671355370605	5.642653754994697
5	1.2202229824688722	3.75364957221238
6	0.7072170319662495	2.6106489856110953
7	0.423508417801945	1.8239150547155283
8	0.28094010238156475	1.3827645737184129
9	0.21063622948865238	1.1663263906101857
>10	0.7841939098357043	7.930914869204611
>50	0.030540305329351893	1.2640502824513964
>100	0.008556875737807413	0.8038749576302058
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.5123513736687526E-5	0.0
14-15	0.0	0.0	0.0	3.0247027473375053E-5	0.0
16-17	0.0	0.0	0.0	6.0494054946750106E-5	0.0
18-19	0.0	0.0	0.0	9.074108242012516E-5	0.0
20-21	0.0	0.0	0.0	9.074108242012516E-5	0.0
22-23	0.0	0.0	0.0	1.0586459615681269E-4	0.0
24-25	0.0	0.0	0.0	2.87346760997063E-4	0.0
26-27	0.0	0.0	0.0	4.385818983639383E-4	0.0
28-29	0.0	0.0	0.0	0.001240128126408377	0.0
30-31	0.0	0.0	0.0	0.004824400882003321	0.0
32-33	0.0	0.0	0.0	0.014730302379533652	0.0
34-35	0.0	0.0	0.0	0.027494547973297926	0.0
36-37	0.0	0.0	0.0	0.05081500615527009	0.0
38-39	0.0	0.0	0.0	0.08954632483492685	0.0
40-41	0.0	0.0	0.0	0.13537057145709006	0.0
42-43	0.0	0.0	0.0	0.1826920459391853	0.0
44-45	0.0	0.0	0.0	0.23804410621546168	0.0
46-47	0.0	0.0	0.0	0.30413386124478614	0.0
48-49	0.0	0.0	0.0	0.3794792066809634	0.0
50-51	0.0	0.0	0.0	0.45886253028483626	0.0
52-53	0.0	0.0	0.0	0.5426921669272953	0.0
54-55	0.0	0.0	0.0	0.6317696628363847	0.0
56-57	0.0	0.0	0.0	0.7403564914658012	0.0
58-59	0.0	0.0	0.0	0.8594390386284788	0.0
60-61	0.0	0.0	0.0	0.9859774780633433	0.0
62-63	0.0	0.0	0.0	1.1149810502372879	0.0
64-65	0.0	0.0	0.0	1.2517278614444165	0.0
66-67	0.0	0.0	0.0	1.4044451031574874	0.0
68-69	0.0	0.0	0.0	1.5804676795387933	0.0
70-71	0.0	0.0	0.0	1.7715381520881035	0.0
72-73	0.0	0.0	0.0	1.9731799607393583	0.0
74-75	0.0	0.0	0.0	2.1771205434785896	0.0
76-77	0.0	0.0	0.0	2.394702535608313	0.0
78-79	0.0	0.0	0.0	2.6472652150109948	0.0
80-81	0.0	0.0	0.0	2.9246153334281075	0.0
82-83	0.0	0.0	0.0	3.213232469579052	0.0
84-85	0.0	0.0	0.0	3.4985375562216623	0.0
86-87	0.0	0.0	0.0	3.80156740096367	0.0
88	0.0	0.0	0.0	4.051619577086062	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	3830	0.0	32.13837	4
CAACGCA	3920	0.0	31.51987	5
ATCAACG	4045	0.0	30.435677	3
GTATCAA	4225	0.0	30.333836	1
AACGCAG	4195	0.0	29.441133	6
GGTATCA	2865	0.0	28.451593	1
TAGACCG	110	1.02182596E-4	25.625557	5
ACGCAGA	4785	0.0	25.614704	7
GTACATG	7735	0.0	25.340672	1
CGCAGAG	4780	0.0	25.248526	8
TATCAAC	5160	0.0	24.055305	2
TACATGG	7905	0.0	23.553204	2
ACATGGG	8210	0.0	21.291193	3
GCAGAGT	6155	0.0	18.768858	9
GAGTACA	5835	0.0	18.734076	1
CTGTCGC	745	0.0	18.279844	9
CATGGGG	5205	0.0	17.059347	4
GTACTAG	710	0.0	16.59078	1
ATTATAC	2045	0.0	16.543957	3
TACCGCG	200	3.0137997E-4	16.436102	8
>>END_MODULE