FastQCFastQC Report
Wed 25 May 2016
SRR1033146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306110
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40560.12268194343200921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35250.10662077184364707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43600.045.3390661
GTATCAA74750.040.486441
TCAACGC87100.034.1707734
ATCAACG87550.033.887733
CAACGCA88350.033.474445
AACGCAG92400.031.9907596
TATCAAC97000.030.6832392
ACGCAGA105200.027.8303187
CGCAGAG107200.027.2656088
GTACATG79350.024.4376851
GCAGAGT122700.023.591519
TACATGG79700.022.6538562
ACATGGG81550.021.5633813
GAGTACT79100.020.91152812-13
AGAGTAC108600.020.12068210-11
GAGTACA60750.019.6012611
CAGAGTA121350.019.0521910-11
GTACTTT90900.018.17109114-15
AGTACTT86850.018.07158512-13
CATGGGG53250.017.8361454
Produced by FastQC (version 0.11.5)