##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033146_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3306110 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.80036810632435 34.0 31.0 34.0 31.0 34.0 2 32.87931617520288 34.0 31.0 34.0 31.0 34.0 3 32.983178720611235 34.0 31.0 34.0 31.0 34.0 4 36.3940634159178 37.0 37.0 37.0 35.0 37.0 5 36.33866417027867 37.0 37.0 37.0 35.0 37.0 6 36.41888170690025 37.0 37.0 37.0 35.0 37.0 7 36.38546721070987 37.0 37.0 37.0 35.0 37.0 8 36.3772796428431 37.0 37.0 37.0 35.0 37.0 9 38.20429326307957 39.0 39.0 39.0 37.0 39.0 10-11 38.18872980632828 39.0 39.0 39.0 37.0 39.0 12-13 38.164917077774184 39.0 39.0 39.0 37.0 39.0 14-15 39.71791123102378 41.0 40.0 41.0 37.0 41.0 16-17 39.7288030343818 41.0 40.0 41.0 37.0 41.0 18-19 39.7401213208272 41.0 40.0 41.0 37.0 41.0 20-21 39.73645099527844 41.0 40.0 41.0 37.0 41.0 22-23 39.67589493392536 41.0 40.0 41.0 37.0 41.0 24-25 39.63631337130344 41.0 40.0 41.0 37.0 41.0 26-27 39.54464446131556 41.0 40.0 41.0 37.0 41.0 28-29 39.457971755325744 41.0 39.5 41.0 36.5 41.0 30-31 39.37547676877055 41.0 39.0 41.0 36.0 41.0 32-33 39.41839079764436 41.0 39.5 41.0 36.0 41.0 34-35 39.372367525581424 41.0 40.0 41.0 36.0 41.0 36-37 39.29262365741006 41.0 39.5 41.0 36.0 41.0 38-39 39.20892907374528 41.0 39.0 41.0 35.0 41.0 40-41 39.105186155330586 41.0 39.0 41.0 35.0 41.0 42-43 39.010029460604756 41.0 39.0 41.0 35.0 41.0 44-45 38.89412133897541 41.0 39.0 41.0 35.0 41.0 46-47 38.720372582884416 41.0 39.0 41.0 35.0 41.0 48-49 38.57037424647093 41.0 39.0 41.0 35.0 41.0 50-51 38.4608795533119 41.0 39.0 41.0 35.0 41.0 52-53 38.33671686664993 41.0 38.0 41.0 35.0 41.0 54-55 38.21740595442983 40.5 38.0 41.0 34.0 41.0 56-57 38.07545408349995 40.0 38.0 41.0 34.0 41.0 58-59 37.90187092988437 40.0 37.0 41.0 34.0 41.0 60-61 37.72082613706138 40.0 37.0 41.0 34.0 41.0 62-63 37.499628717737764 40.0 36.5 41.0 33.5 41.0 64-65 37.258833341903326 39.0 36.0 41.0 33.0 41.0 66-67 36.98944726581995 39.0 35.5 41.0 33.0 41.0 68-69 36.67124687321353 39.0 35.0 41.0 33.0 41.0 70-71 36.33458823209149 37.5 35.0 40.5 32.0 41.0 72-73 35.97723669206408 37.0 35.0 40.0 32.0 41.0 74-75 35.633908883854446 37.0 35.0 39.0 32.0 41.0 76-77 35.28032113269069 36.0 35.0 39.0 31.5 41.0 78-79 34.92272262568396 36.0 35.0 38.5 31.0 39.5 80-81 34.56344571112274 35.0 35.0 37.0 31.0 39.0 82-83 34.22306729056202 35.0 35.0 37.0 31.0 39.0 84-85 33.93708315815263 35.0 34.5 36.5 31.0 38.0 86-87 33.69227778265091 35.0 34.0 36.0 31.0 37.0 88-89 33.46153772862972 35.0 34.0 36.0 31.0 37.0 90-91 33.25575782414983 35.0 34.0 35.5 30.0 36.5 92-93 33.08910456699868 35.0 34.0 35.0 30.0 36.0 94-95 32.94348978104176 35.0 34.0 35.0 30.0 36.0 96-97 32.823793219221386 35.0 34.0 35.0 30.0 36.0 98-99 32.71970699704487 35.0 34.0 35.0 30.0 36.0 100 32.6255563184528 35.0 34.0 35.0 30.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13194248928857633 1101 2 0.1711033044697956 1101 3 0.17557028913700634 1101 4 0.1170804669815908 1101 5 0.1083883520101736 1101 6 0.11986714547878563 1101 7 0.13586842313735303 1101 8 0.13907351073356722 1101 9 0.15162424847045486 1101 10-11 0.15494856150117897 1101 12-13 0.1685189980393389 1101 14-15 0.21776909499138952 1101 16-17 0.23466669808686902 1101 18-19 0.20484616561114422 1101 20-21 0.1851484174684046 1101 22-23 0.22464238601947528 1101 24-25 0.213245207150365 1101 26-27 0.22515097964775777 1101 28-29 0.19255106195850402 1101 30-31 0.1736476011344763 1101 32-33 0.1597028356268595 1101 34-35 0.2111898906784262 1101 36-37 0.24202983579741044 1101 38-39 0.15262766019760932 1101 40-41 0.13032725681657809 1101 42-43 0.09499193656676397 1101 44-45 0.0628808509659109 1101 46-47 0.1902909987549819 1101 48-49 0.20087622448381381 1101 50-51 0.20679373217498664 1101 52-53 0.2073533427932972 1101 54-55 0.2327084921078395 1101 56-57 0.18004885084383204 1101 58-59 0.15788835724325878 1101 60-61 0.1760529355235505 1101 62-63 0.1532937126000533 1101 64-65 0.17204093594637015 1101 66-67 0.17324910167696572 1101 68-69 0.16816718817786835 1101 70-71 0.1940548606193957 1101 72-73 0.20482116581352727 1101 74-75 0.1812611424366466 1101 76-77 0.21282669036721558 1101 78-79 0.11107975273394288 1101 80-81 0.1389596455160742 1101 82-83 0.1581131413121284 1101 84-85 0.1622939720666423 1101 86-87 0.1292195753696177 1101 88-89 0.14832115536866297 1101 90-91 0.19832731415709048 1101 92-93 0.17256025613346537 1101 94-95 0.22464601992641775 1101 96-97 0.18973437076847688 1101 98-99 0.13989065940533152 1101 100 0.23045195556668574 1102 1 0.11172569886374362 1102 2 0.1284050840035036 1102 3 0.09950119595291795 1102 4 0.062495478112488456 1102 5 0.05720367943484206 1102 6 0.10312346744586165 1102 7 0.12972487364372398 1102 8 0.14056276160800962 1102 9 0.18410109111090378 1102 10-11 0.13464916812574046 1102 12-13 0.15724545763707454 1102 14-15 0.1962954209473864 1102 16-17 0.18782171358936495 1102 18-19 0.19839932301961483 1102 20-21 0.21561569456727625 1102 22-23 0.2126402372397891 1102 24-25 0.1816340770010072 1102 26-27 0.27415112188309365 1102 28-29 0.27017208355343314 1102 30-31 0.2538923993545268 1102 32-33 0.2295107450428162 1102 34-35 0.2238958549615404 1102 36-37 0.1940566316670811 1102 38-39 0.18333701549368442 1102 40-41 0.20387970544616962 1102 42-43 0.20104280770779326 1102 44-45 0.24142567806462267 1102 46-47 0.2388357577572151 1102 48-49 0.24185294670858326 1102 50-51 0.1897008678518617 1102 52-53 0.22085644353302314 1102 54-55 0.22649382495049508 1102 56-57 0.2581893478886741 1102 58-59 0.2549959064288245 1102 60-61 0.3066018996274025 1102 62-63 0.29547365170261486 1102 64-65 0.26910585289792266 1102 66-67 0.2320173964921466 1102 68-69 0.23732657451540007 1102 70-71 0.16488043359135673 1102 72-73 0.26005578073224456 1102 74-75 0.2303448946832276 1102 76-77 0.21801405975504906 1102 78-79 0.2976615711843138 1102 80-81 0.27807464349763933 1102 82-83 0.21952293222427954 1102 84-85 0.21373578079360556 1102 86-87 0.23560987390363408 1102 88-89 0.29526468314814736 1102 90-91 0.2529698493488226 1102 92-93 0.2742651091011723 1102 94-95 0.26156589424908816 1102 96-97 0.2801220440392598 1102 98-99 0.27075022978559105 1102 100 0.33622204860854055 1103 1 0.09808733825047966 1103 2 0.10666337804903492 1103 3 0.10797349889818264 1103 4 0.05914481093508783 1103 5 0.049356864752546414 1103 6 0.09641776067365981 1103 7 0.08878416199558359 1103 8 0.0934208457309964 1103 9 0.13705825235146563 1103 10-11 0.12417471664899438 1103 12-13 0.09873721016476367 1103 14-15 0.12141347040267902 1103 16-17 0.13686095626807315 1103 18-19 0.14968677899599925 1103 20-21 0.16715363424233942 1103 22-23 0.14530645131473818 1103 24-25 0.160603005161029 1103 26-27 0.17611926789206223 1103 28-29 0.1387760567319134 1103 30-31 0.1408908069108108 1103 32-33 0.154643911898674 1103 34-35 0.1838030201675167 1103 36-37 0.16831421031069738 1103 38-39 0.10632016238294995 1103 40-41 0.1969967297091344 1103 42-43 0.14636214651455504 1103 44-45 0.11952837917198877 1103 46-47 0.15714841862670426 1103 48-49 0.16216840732065663 1103 50-51 0.06439271127001689 1103 52-53 0.10435124233320892 1103 54-55 0.04556956568568182 1103 56-57 0.052624376905946235 1103 58-59 0.061636352545640705 1103 60-61 0.026613128367394268 1103 62-63 -0.01429381709332489 1103 64-65 -0.014916287349237223 1103 66-67 0.01865573082439198 1103 68-69 0.03616716292183497 1103 70-71 0.017452961646334586 1103 72-73 0.021295044761366455 1103 74-75 -0.008942182660419462 1103 76-77 -0.037310323611997376 1103 78-79 -0.024583730269441162 1103 80-81 -0.026317728866736445 1103 82-83 -0.0632495339191479 1103 84-85 -0.09140708910767614 1103 86-87 -0.09432269551255956 1103 88-89 -0.0575874935598506 1103 90-91 -0.07739279848481573 1103 92-93 -0.056686861521768606 1103 94-95 -0.029680619155051602 1103 96-97 -0.010897731613866313 1103 98-99 -2.8044230560908545E-5 1103 100 0.0310338265055492 1104 1 0.04767068058396262 1104 2 0.04145401501718737 1104 3 0.0707740301358939 1104 4 0.0465383228444054 1104 5 -0.0030191410546720476 1104 6 0.015832220995456225 1104 7 0.03143130456378884 1104 8 0.06789878817325956 1104 9 0.06465557992994775 1104 10-11 0.06908950326698005 1104 12-13 0.0811865327974175 1104 14-15 0.08958437237439654 1104 16-17 0.07322403328378613 1104 18-19 0.07133196640521788 1104 20-21 0.0781511580567269 1104 22-23 0.06559945679402546 1104 24-25 0.04187550143497276 1104 26-27 -0.0105054024571416 1104 28-29 -2.945260128157656E-4 1104 30-31 -0.019733390848905685 1104 32-33 0.046768489628192356 1104 34-35 0.033185871976677106 1104 36-37 -0.031847334127434124 1104 38-39 -0.009165979683203318 1104 40-41 -0.01157802026681054 1104 42-43 -0.018067116592767718 1104 44-45 0.013879102096439055 1104 46-47 -0.023926008980367897 1104 48-49 -0.048832454729684116 1104 50-51 -0.08839749761847315 1104 52-53 -0.007621962759493783 1104 54-55 -0.0330888954660864 1104 56-57 0.008144280974207163 1104 58-59 0.023054003173719195 1104 60-61 -0.019425269200795015 1104 62-63 0.030005163173775884 1104 64-65 -0.03711699649527844 1104 66-67 0.0010401869504832462 1104 68-69 0.04332499537250811 1104 70-71 0.03747766459242996 1104 72-73 0.08968993582032425 1104 74-75 0.06307816356047624 1104 76-77 0.030746947425924986 1104 78-79 0.056903264028719036 1104 80-81 0.02969086676358046 1104 82-83 0.02561083814362064 1104 84-85 -0.0025409569514991404 1104 86-87 0.06958593369648014 1104 88-89 0.08703365920503359 1104 90-91 0.07914330388251045 1104 92-93 0.053453782941055294 1104 94-95 -0.026967615155051305 1104 96-97 -0.006750398897125365 1104 98-99 -0.03856987658172528 1104 100 -0.05546444906647707 1105 1 0.12638209385240629 1105 2 0.11417071325148953 1105 3 0.0943878346005178 1105 4 0.0633755383698329 1105 5 0.04544453943903193 1105 6 0.05166650553901775 1105 7 -0.008159612319253995 1105 8 0.0012324559981067296 1105 9 0.08069499139213576 1105 10-11 0.016401466773956486 1105 12-13 0.052126725715389455 1105 14-15 0.09342646729472648 1105 16-17 0.09964137834105458 1105 18-19 0.09312410919964265 1105 20-21 0.11124917661263822 1105 22-23 0.15612473389933967 1105 24-25 0.10651387430101522 1105 26-27 0.06675172534788487 1105 28-29 0.08114798953059221 1105 30-31 0.19975423087464605 1105 32-33 0.18235217731346864 1105 34-35 0.15514900264996356 1105 36-37 0.1305638897351855 1105 38-39 0.10663889051318876 1105 40-41 0.16821415597677003 1105 42-43 0.11916745394103856 1105 44-45 0.22106974971381277 1105 46-47 0.2579735474002618 1105 48-49 0.19596858553891394 1105 50-51 0.18637082519929038 1105 52-53 0.20598943754740162 1105 54-55 0.253613729037923 1105 56-57 0.22032351406879513 1105 58-59 0.2047193109686205 1105 60-61 0.1940338538460864 1105 62-63 0.18762441985001743 1105 64-65 0.11453275744440816 1105 66-67 0.09765814329097111 1105 68-69 0.10376773669766948 1105 70-71 0.1666912959576976 1105 72-73 0.09924629554922149 1105 74-75 0.09297181616595651 1105 76-77 0.05121057356476655 1105 78-79 0.09632398745023352 1105 80-81 0.13589532888033062 1105 82-83 0.13470595498568372 1105 84-85 0.11233484216962353 1105 86-87 0.09770747118670897 1105 88-89 0.09994668305009213 1105 90-91 0.11298059048738196 1105 92-93 0.12908643182113622 1105 94-95 0.1444412212744126 1105 96-97 0.19973244377897004 1105 98-99 0.15272140698763081 1105 100 0.12452668690850288 1106 1 0.03915363848921771 1106 2 -0.0020619978424250007 1106 3 0.03198108991212223 1106 4 0.0033223417357248763 1106 5 -0.0120781094309379 1106 6 2.2777838939447292E-4 1106 7 0.024517951597310628 1106 8 0.04964090942686994 1106 9 0.030082746494173307 1106 10-11 0.07100329802779015 1106 12-13 0.0559835622888869 1106 14-15 0.08485237889158981 1106 16-17 0.07000142283278166 1106 18-19 0.04627391414531701 1106 20-21 0.04687770290468052 1106 22-23 0.06600496087164487 1106 24-25 0.09453544311811868 1106 26-27 0.02107943273415458 1106 28-29 0.048475987080315974 1106 30-31 0.10684171515745788 1106 32-33 0.09603153057135927 1106 34-35 0.08237560419105705 1106 36-37 0.08032885733359763 1106 38-39 0.07168571967138604 1106 40-41 0.036123447655526775 1106 42-43 0.0923224173482069 1106 44-45 0.1047564376591339 1106 46-47 0.18390150626153456 1106 48-49 0.1567216403153182 1106 50-51 0.11584353371397071 1106 52-53 0.1245425697007363 1106 54-55 0.10983684861751186 1106 56-57 0.1715434902109223 1106 58-59 0.06514972009910025 1106 60-61 0.09323058467111878 1106 62-63 0.06092712481825657 1106 64-65 0.12924654343272834 1106 66-67 0.1538455566449457 1106 68-69 0.1288877515282536 1106 70-71 0.12904105576684088 1106 72-73 0.17072830541477657 1106 74-75 0.0949246162433326 1106 76-77 0.04032678403000034 1106 78-79 0.10499882705431673 1106 80-81 0.08040011657740109 1106 82-83 0.08790911776738142 1106 84-85 0.10085275170310837 1106 86-87 0.11596681283806021 1106 88-89 0.12320860393218425 1106 90-91 0.10175279605207521 1106 92-93 0.07884245271290524 1106 94-95 0.04432306026593835 1106 96-97 0.04379384559088351 1106 98-99 0.054661955718970034 1106 100 0.09809682586455892 1107 1 -0.039028163273698624 1107 2 -0.04037240632192152 1107 3 -0.025581734622520003 1107 4 -0.024509103859152503 1107 5 -0.1161878344407512 1107 6 -0.11011774304413535 1107 7 -0.04347829793303504 1107 8 -0.010394892615757101 1107 9 -0.012373770996546796 1107 10-11 -0.040102204461973656 1107 12-13 -0.012086261805194454 1107 14-15 0.011279841202430418 1107 16-17 0.026964586694155912 1107 18-19 0.02614136261263411 1107 20-21 -0.0029505497895456756 1107 22-23 0.00810720130490239 1107 24-25 0.019918910734197937 1107 26-27 0.07945420168725548 1107 28-29 0.026685972165502392 1107 30-31 0.031062056330405596 1107 32-33 0.004844011256352587 1107 34-35 0.05480281766434558 1107 36-37 0.04731952222073943 1107 38-39 0.03746652389211391 1107 40-41 0.07253965361768167 1107 42-43 0.05900371402646698 1107 44-45 0.1110141926072501 1107 46-47 0.05974397650213348 1107 48-49 -0.02614314287338715 1107 50-51 0.019355693565245247 1107 52-53 0.05954551113963191 1107 54-55 0.02037494515227678 1107 56-57 0.03538130462688116 1107 58-59 -0.009502470767323246 1107 60-61 0.010805539264438835 1107 62-63 0.008944389698420707 1107 64-65 -0.0960096920935456 1107 66-67 -0.05815357674588029 1107 68-69 0.0383826059011696 1107 70-71 0.08880958513383774 1107 72-73 0.07038354114513368 1107 74-75 0.028009505660349987 1107 76-77 4.8288714135935606E-4 1107 78-79 0.03697099043746732 1107 80-81 -0.01927553016096084 1107 82-83 0.008846215741151298 1107 84-85 0.013009623710146911 1107 86-87 0.00956151512986736 1107 88-89 0.028458227202634134 1107 90-91 -0.0030703880435609676 1107 92-93 -0.007132753527422153 1107 94-95 -0.04398335785956675 1107 96-97 0.0017636283458273283 1107 98-99 0.05609126655936336 1107 100 0.019936944034604664 1108 1 0.032153721603769725 1108 2 0.016902123772233324 1108 3 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-0.08402181169078915 2214 20-21 -0.10955165095964503 2214 22-23 -0.1677086270677961 2214 24-25 -0.06851798867377568 2214 26-27 -0.10245912536470314 2214 28-29 -0.07458608438862768 2214 30-31 -0.005016817071201274 2214 32-33 -0.033273764338360934 2214 34-35 -0.12425612570303457 2214 36-37 -0.1571795340207558 2214 38-39 -0.18087713108443637 2214 40-41 -0.10994683855173548 2214 42-43 -0.08245781008191244 2214 44-45 -0.11164480272672961 2214 46-47 -0.06123928573722992 2214 48-49 -0.17608801809866037 2214 50-51 -0.05568732833411616 2214 52-53 -0.09595351907714189 2214 54-55 -0.11377199317748676 2214 56-57 -0.08014322084134307 2214 58-59 -0.1567972080374247 2214 60-61 -0.18508908533311796 2214 62-63 -0.1644246145288335 2214 64-65 -0.13293637079326004 2214 66-67 -0.17980759680230562 2214 68-69 -0.11748551628136994 2214 70-71 -0.0408966696418247 2214 72-73 -0.15762612279289812 2214 74-75 -0.06899327152931534 2214 76-77 -0.028156816626918157 2214 78-79 -0.25462263846258537 2214 80-81 -0.1491760384161509 2214 82-83 -0.1543274146320357 2214 84-85 -0.2108345145875603 2214 86-87 -0.0914756976654374 2214 88-89 -0.20701483628035078 2214 90-91 -0.11085918632762315 2214 92-93 -0.07957449422360696 2214 94-95 -0.139351409871999 2214 96-97 -0.19321333149333952 2214 98-99 -0.2712558059392194 2214 100 -0.43482567289801466 2215 1 -0.058073151968322634 2215 2 -0.0940243123928397 2215 3 -0.09678915775268138 2215 4 -0.07175411391168751 2215 5 -0.05830325200140862 2215 6 -0.05606720834611423 2215 7 -0.10144158546603421 2215 8 -0.09723346244077646 2215 9 -0.12002240597691127 2215 10-11 -0.10722432634034362 2215 12-13 -0.08748882397451752 2215 14-15 -0.13212436712947095 2215 16-17 -0.16791071505151223 2215 18-19 -0.1615831806563648 2215 20-21 -0.15921449571222723 2215 22-23 -0.16808408317113077 2215 24-25 -0.1583143051305882 2215 26-27 -0.12114665587631634 2215 28-29 -0.0923594891706756 2215 30-31 -0.08657705663510029 2215 32-33 -0.1834042051553979 2215 34-35 -0.11966841117150295 2215 36-37 -0.06552036099981251 2215 38-39 -0.10496368321872751 2215 40-41 -0.16976909831338105 2215 42-43 -0.15856786942068624 2215 44-45 -0.1409930270267452 2215 46-47 -0.20922535529435748 2215 48-49 -0.19580419928480097 2215 50-51 -0.19921395129806285 2215 52-53 -0.3030534961350071 2215 54-55 -0.26202280107359144 2215 56-57 -0.29182113138138277 2215 58-59 -0.27831845092898533 2215 60-61 -0.27378351705601034 2215 62-63 -0.26571914260124885 2215 64-65 -0.216008742015525 2215 66-67 -0.22830207044751205 2215 68-69 -0.2292894104514076 2215 70-71 -0.1642895906135422 2215 72-73 -0.10496126970611641 2215 74-75 -0.1530277760999752 2215 76-77 -0.19461870031092587 2215 78-79 -0.18433149871900412 2215 80-81 -0.20744605183599418 2215 82-83 -0.13224691238629305 2215 84-85 -0.10818305163352449 2215 86-87 -0.20407046396211825 2215 88-89 -0.1867557139970586 2215 90-91 -0.2113059821858556 2215 92-93 -0.18146068292382012 2215 94-95 -0.1407193329577936 2215 96-97 -0.0987044683149918 2215 98-99 -0.12829524573024287 2215 100 -0.061513786063024156 2216 1 -0.18643620423357277 2216 2 -0.19207054209424967 2216 3 -0.24971384342030234 2216 4 -0.17193970893576704 2216 5 -0.14638079834556805 2216 6 -0.15378587113799114 2216 7 -0.13458072628468187 2216 8 -0.14879455211166004 2216 9 -0.2551120820208226 2216 10-11 -0.2541747001266259 2216 12-13 -0.20507313429684615 2216 14-15 -0.3894162003882613 2216 16-17 -0.3453888362946316 2216 18-19 -0.28567560569439365 2216 20-21 -0.3503530602598417 2216 22-23 -0.3491748655597675 2216 24-25 -0.4415299799797836 2216 26-27 -0.48734874335458755 2216 28-29 -0.4761287171657358 2216 30-31 -0.4325291270725913 2216 32-33 -0.4538531099729255 2216 34-35 -0.3972976682432048 2216 36-37 -0.4462398085801951 2216 38-39 -0.4315712267174021 2216 40-41 -0.499203271399999 2216 42-43 -0.4680280451959433 2216 44-45 -0.4140621209550872 2216 46-47 -0.4585992993717767 2216 48-49 -0.36912271799632634 2216 50-51 -0.3402147010923926 2216 52-53 -0.2773788621876463 2216 54-55 -0.27926001446198967 2216 56-57 -0.30061255330122094 2216 58-59 -0.33535782888355214 2216 60-61 -0.2450785981018342 2216 62-63 -0.2595626690275523 2216 64-65 -0.2140631506620565 2216 66-67 -0.23358140801526872 2216 68-69 -0.20192767632118347 2216 70-71 -0.17068600403753464 2216 72-73 -0.18730962076595148 2216 74-75 -0.13616877991805154 2216 76-77 -0.15483889488485403 2216 78-79 -0.14495530645443466 2216 80-81 -0.17581723080686373 2216 82-83 -0.19815646489697514 2216 84-85 -0.1556602545941601 2216 86-87 -0.20163986336571327 2216 88-89 -0.18723474401885198 2216 90-91 -0.17889451257952516 2216 92-93 -0.16193699963800157 2216 94-95 -0.1349198188859475 2216 96-97 -0.15260045957450075 2216 98-99 -0.09575981742887052 2216 100 -0.11757408229126298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33.0 3 7.0 4 12.0 5 22.0 6 43.0 7 131.0 8 312.0 9 599.0 10 1147.0 11 1911.0 12 2967.0 13 4472.0 14 5534.0 15 7232.0 16 8413.0 17 11981.0 18 14203.0 19 7686.0 20 5396.0 21 5176.0 22 5350.0 23 5801.0 24 6813.0 25 8313.0 26 10524.0 27 13430.0 28 17525.0 29 22318.0 30 29099.0 31 37271.0 32 47905.0 33 62931.0 34 87071.0 35 132279.0 36 243798.0 37 592106.0 38 1219563.0 39 683752.0 40 2984.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.322603107804326 20.20272633005094 13.724687592246385 23.74998296989835 2 15.254733037937198 20.473771116089836 40.801689256939284 23.469806589033684 3 17.806152850328633 25.787859448112737 30.676777239716767 25.729210461841863 4 11.883927426360454 17.087877453340848 40.094171266239655 30.93402385405904 5 12.01702686984817 38.23402505496394 36.73533856153934 13.013609513648547 6 30.105214444187347 39.237531778331295 17.812319935392253 12.844933842089103 7 26.280274775633174 32.993977561804854 23.254385248504978 17.471362414056994 8 24.444934448364474 36.66136736501299 20.811948594354188 18.08174959226834 9 24.801367410099623 16.346400476210064 21.72877804207006 37.12345407162025 10-11 23.31732754200953 26.85225318691689 29.778545383602108 20.051873887471473 12-13 24.3734177023753 24.20904023157124 29.80049060678562 21.61705145926784 14-15 21.4920048752181 26.238960397095255 27.09914087816537 25.169893849521273 16-17 20.62212086107238 29.692296989513355 27.60213665002072 22.083445499393548 18-19 20.37905015749646 28.40376261378189 30.269688872649574 20.947498356072067 20-21 21.65682630039533 27.71055711999903 29.861498861199415 20.771117718406224 22-23 21.165787660050764 27.498764026914497 29.94020597383715 21.395242339197594 24-25 21.1658129430251 27.66873707882841 29.779498836243857 21.38595114190263 26-27 20.92745751802227 27.614850838568938 29.88803251252477 21.569659130884027 28-29 20.9581215087897 27.654575198393612 29.976765093614198 21.41053819920249 30-31 21.24127904118055 28.15111512535563 29.51522702292582 21.092378810537998 32-33 20.861972045752555 27.76597167301312 30.039788119414577 21.33226816181974 34-35 21.07677819757877 27.535889202794134 30.026012533517893 21.3613200661092 36-37 21.441721615068516 28.00227942328836 29.386664496363636 21.169334465279487 38-39 21.286991891195616 28.110032737465374 29.491417326062646 21.111558045276364 40-41 21.08858367301492 27.849376833554405 29.643068075094643 21.418971418336028 42-43 21.09352356483787 27.729172427605796 29.5212304095294 21.65607359802693 44-45 21.292379948093267 27.915215673733947 29.36129485774811 21.43110952042468 46-47 21.374650723329616 28.109849961345684 29.103396785104223 21.41210253022048 48-49 21.29205212700903 28.13687189242569 28.885313955284055 21.685762025281228 50-51 21.352205410037673 28.05294514716623 28.7406682090902 21.8541812337059 52-53 21.37523405053374 28.05173159583897 28.810569555735032 21.76246479789226 54-55 21.540475383852193 28.362820481796515 28.619221043206817 21.477483091144478 56-57 21.332994556891 28.09379267789166 28.736938580133486 21.836274185083855 58-59 21.14022959579513 27.730665796348596 29.14237171554997 21.986732892306303 60-61 21.47428902765307 28.167062073504688 28.6426694264234 21.71597947241884 62-63 21.275214333937477 28.120848527671644 28.709601410260444 21.894335728130436 64-65 21.280166605414678 27.79078437333164 28.917185796192697 22.01186322506099 66-67 21.150833068166282 27.966659729977266 28.701817735259638 22.180689466596814 68-69 21.64868859163041 27.982277415077267 28.633293245381818 21.735740747910505 70-71 21.634844128798477 27.832715030874795 28.610399882641 21.92204095768573 72-73 21.424791608094214 27.965795249706566 28.72259546434074 21.886817677858485 74-75 21.46409571310629 28.011757700981082 28.537802300210657 21.98634428570197 76-77 21.438885717975868 28.057029853667153 28.377604622737472 22.126479805619503 78-79 21.620778383289842 28.091693286579485 28.430777690632702 21.85675063949797 80-81 21.497772745984758 27.88268857855914 28.54681165570558 22.072727019750527 82-83 21.348340775652712 27.910058791449604 28.70621001889542 22.03539041400227 84-85 21.339092983628614 27.86305691881697 28.552539265634003 22.245310831920417 86-87 21.469604805531826 27.976609343951864 28.3697908703183 22.18399498019801 88-89 21.198719945797325 27.87844324599 28.474959998306165 22.447876809906507 90-91 21.224874920941808 27.683146562923366 28.603246293452518 22.488732222682316 92-93 21.245669758619574 27.957729161379735 28.36373513395959 22.432865946041105 94-95 21.000571668819244 27.701634246894386 28.5864807886005 22.711313295685866 96-97 20.936977293556474 27.656989634343688 28.606141356458192 22.799891715641646 98-99 21.01307881467949 27.715457138449718 28.486196769012523 22.785267277858267 100 21.132085744273482 27.617834857279398 28.398631624477105 22.851447773970012 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 31.0 1 20.0 2 69.0 3 144.0 4 109.5 5 77.5 6 153.5 7 351.5 8 662.5 9 863.0 10 794.5 11 717.5 12 777.0 13 877.5 14 1068.0 15 1259.5 16 1413.5 17 1630.0 18 1977.0 19 2351.5 20 2875.0 21 3704.0 22 5082.0 23 7217.5 24 10006.5 25 13693.0 26 18685.0 27 25401.5 28 34064.0 29 44021.5 30 54400.5 31 65856.0 32 77741.5 33 88603.5 34 99036.0 35 108168.0 36 115642.5 37 122403.0 38 127065.5 39 129808.0 40 131528.5 41 131016.5 42 128073.0 43 124583.0 44 121399.0 45 117308.0 46 114249.5 47 110997.5 48 106580.5 49 103466.5 50 99399.5 51 93880.5 52 86452.5 53 80058.5 54 76424.5 55 72608.5 56 66899.0 57 60407.5 58 54492.5 59 48785.0 60 42944.0 61 36128.5 62 29985.0 63 24736.5 64 19831.5 65 15205.0 66 11518.0 67 8437.0 68 5955.0 69 4415.0 70 2935.5 71 2000.0 72 1536.5 73 1076.0 74 761.5 75 531.0 76 301.0 77 163.0 78 109.5 79 59.5 80 31.0 81 19.0 82 5.5 83 2.5 84 2.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0948244311290308 2 0.0012401281264083773 3 0.0 4 1.8148216484025032E-4 5 0.036780385407624065 6 1.5123513736687525E-4 7 0.004234583846272507 8 4.2345838462725077E-4 9 1.8148216484025032E-4 10-11 4.537054121006258E-4 12-13 0.0 14-15 4.537054121006258E-5 16-17 0.0 18-19 1.2098810989350021E-4 20-21 0.0 22-23 3.1759378847043805E-4 24-25 1.5123513736687525E-4 26-27 0.0018450686758758783 28-29 0.009316084461799518 30-31 0.03667452081146726 32-33 0.025770467407315548 34-35 0.02941523421785724 36-37 0.026859360396357047 38-39 0.02648127255293986 40-41 0.03188036695693731 42-43 0.024318610088593543 44-45 0.02294237033855498 46-47 0.015985554019678716 48-49 0.011388005843725709 50-51 0.008272562013968077 52-53 0.006049405494675011 54-55 0.0045370541210062575 56-57 0.00287346760997063 58-59 0.0011796340714616271 60-61 6.503110906775636E-4 62-63 0.0016333394835622529 64-65 0.00287346760997063 66-67 5.44446494520751E-4 68-69 0.0013913632637752526 70-71 4.537054121006258E-4 72-73 5.898170357308136E-4 74-75 6.503110906775636E-4 76-77 5.141994670473759E-4 78-79 3.0247027473375053E-5 80-81 6.351875769408761E-4 82-83 5.141994670473759E-4 84-85 1.9660567857693786E-4 86-87 1.0586459615681269E-4 88-89 0.0 90-91 3.0247027473375053E-5 92-93 3.0247027473375053E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3306110.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.200880763574695 #Duplication Level Percentage of deduplicated Percentage of total 1 72.07690796648505 42.67016434331028 2 15.738783122114341 18.634996459521062 3 5.63563545941355 10.00903748579145 4 2.524667602895324 5.978501829066641 5 1.2911624421013523 3.8218976890624043 6 0.7929355604184667 2.816549013931915 7 0.46822493575284974 1.9403530014425816 8 0.31119165452408803 1.4738256027280052 9 0.2214469148710217 1.1798867162466748 >10 0.8941515845365453 8.7245356186365 >50 0.03332346208594356 1.337113610587326 >100 0.011262420143347995 1.0710190367239634 >500 1.022915560400703E-4 0.031187113586981695 >1k 2.0458310202396582E-4 0.310932479364188 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4056 0.12268194343200921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3525 0.10662077184364707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.0494054946750106E-5 0.0 18-19 0.0 0.0 0.0 9.074108242012516E-5 0.0 20-21 0.0 0.0 0.0 9.074108242012516E-5 0.0 22-23 0.0 0.0 0.0 1.0586459615681269E-4 0.0 24-25 0.0 0.0 0.0 2.87346760997063E-4 0.0 26-27 0.0 0.0 0.0 4.083348708905632E-4 0.0 28-29 0.0 0.0 0.0 0.0011796340714616271 0.0 30-31 0.0 0.0 0.0 0.004779030340793258 0.0 32-33 0.0 0.0 0.0 0.014715178865796964 0.0 34-35 0.0 0.0 0.0 0.02753991851450799 0.0 36-37 0.0 0.0 0.0 0.050966241292636966 0.0 38-39 0.0 0.0 0.0 0.09000003024702748 0.0 40-41 0.0 0.0 0.0 0.13602088254776762 0.0 42-43 0.0 0.0 0.0 0.1836901978458067 0.0 44-45 0.0 0.0 0.0 0.23929935785560674 0.0 46-47 0.0 0.0 0.0 0.3058276947832952 0.0 48-49 0.0 0.0 0.0 0.38164186914530973 0.0 50-51 0.0 0.0 0.0 0.46155451572996664 0.0 52-53 0.0 0.0 0.0 0.5459890929218931 0.0 54-55 0.0 0.0 0.0 0.6356564058667135 0.0 56-57 0.0 0.0 0.0 0.7453321274851714 0.0 58-59 0.0 0.0 0.0 0.8659875200764644 0.0 60-61 0.0 0.0 0.0 0.993977816830051 0.0 62-63 0.0 0.0 0.0 1.1242517641578775 0.0 64-65 0.0 0.0 0.0 1.2618908626754703 0.0 66-67 0.0 0.0 0.0 1.415969220624843 0.0 68-69 0.0 0.0 0.0 1.5932470486462944 0.0 70-71 0.0 0.0 0.0 1.7858147490555365 0.0 72-73 0.0 0.0 0.0 1.9889084150255134 0.0 74-75 0.0 0.0 0.0 2.1940891258911535 0.0 76-77 0.0 0.0 0.0 2.4136069277791723 0.0 78-79 0.0 0.0 0.0 2.669935362102289 0.0 80-81 0.0 0.0 0.0 2.951489817338201 0.0 82-83 0.0 0.0 0.0 3.2444625254453117 0.0 84-85 0.0 0.0 0.0 3.5335484905220937 0.0 86-87 0.0 0.0 0.0 3.8419169356131526 0.0 88 0.0 0.0 0.0 4.0961432015268695 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4360 0.0 45.339066 1 GTATCAA 7475 0.0 40.48644 1 TCAACGC 8710 0.0 34.170773 4 ATCAACG 8755 0.0 33.88773 3 CAACGCA 8835 0.0 33.47444 5 AACGCAG 9240 0.0 31.990759 6 TATCAAC 9700 0.0 30.683239 2 ACGCAGA 10520 0.0 27.830318 7 CGCAGAG 10720 0.0 27.265608 8 GTACATG 7935 0.0 24.437685 1 GCAGAGT 12270 0.0 23.59151 9 TACATGG 7970 0.0 22.653856 2 ACATGGG 8155 0.0 21.563381 3 GAGTACT 7910 0.0 20.911528 12-13 AGAGTAC 10860 0.0 20.120682 10-11 GAGTACA 6075 0.0 19.601261 1 CAGAGTA 12135 0.0 19.05219 10-11 GTACTTT 9090 0.0 18.171091 14-15 AGTACTT 8685 0.0 18.071585 12-13 CATGGGG 5325 0.0 17.836145 4 >>END_MODULE