FastQCFastQC Report
Wed 25 May 2016
SRR1033145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3092240
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.032.2950061
GTATCAA33600.030.9929451
TCAACGC32450.029.965014
ATCAACG33500.029.0328523
GTACATG81250.028.4752771
CAACGCA35400.027.7324165
TACATGG82100.027.0853962
TACACCG4100.026.350495
AACGCAG37400.025.8638296
ACATGGG83350.025.7053433
TATCAAC41750.023.9850372
ACACCGT5900.022.2845176
ACGCAGA43800.021.9774327
GTCGTAC3101.2732926E-1121.2802241
CGCAGAG45550.020.9268978
CATGGGG57850.018.6759744
GTGTAGG11400.018.1868551
GTACCGT2603.7061545E-718.0602556
CGTACAC3752.0190782E-1017.5412653
GAGTACA56450.017.2789061
Produced by FastQC (version 0.11.5)