##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033145_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3092240
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.137388753783664	33.0	31.0	34.0	28.0	34.0
2	31.20602475875094	33.0	31.0	34.0	28.0	34.0
3	31.402780185237887	34.0	31.0	34.0	28.0	34.0
4	34.76756817064652	37.0	35.0	37.0	32.0	37.0
5	34.88103801774765	37.0	35.0	37.0	32.0	37.0
6	34.91835691925594	37.0	35.0	37.0	32.0	37.0
7	34.86309924197346	37.0	35.0	37.0	32.0	37.0
8	34.87559891858329	37.0	35.0	37.0	32.0	37.0
9	36.48547363723385	39.0	37.0	39.0	32.0	39.0
10-11	36.46412180167128	39.0	37.0	39.0	32.0	39.0
12-13	36.4014966496779	39.0	37.0	39.0	32.0	39.0
14-15	37.78078367138385	40.0	38.0	41.0	32.5	41.0
16-17	37.76616611259152	40.0	38.0	41.0	32.0	41.0
18-19	37.73548414741417	40.0	38.0	41.0	32.0	41.0
20-21	37.75121998939281	40.0	38.0	41.0	32.0	41.0
22-23	37.69448652109797	40.0	38.0	41.0	32.0	41.0
24-25	37.679328092256746	40.0	38.0	41.0	32.0	41.0
26-27	37.63347702636277	40.0	38.0	41.0	32.0	41.0
28-29	37.59492390629447	40.0	38.0	41.0	32.0	41.0
30-31	37.58692533567899	40.0	38.0	41.0	32.0	41.0
32-33	37.662518433239335	40.0	38.0	41.0	32.0	41.0
34-35	37.62888666468321	40.0	38.0	41.0	32.0	41.0
36-37	37.54650803301167	40.0	38.0	41.0	31.0	41.0
38-39	37.46473397925129	40.0	38.0	41.0	31.0	41.0
40-41	37.39790782733553	40.0	38.0	41.0	31.0	41.0
42-43	37.27116831164463	40.0	38.0	41.0	31.0	41.0
44-45	37.12307210953871	40.0	38.0	41.0	30.0	41.0
46-47	36.98961270793988	40.0	37.0	41.0	30.0	41.0
48-49	36.8683153636199	40.0	37.0	41.0	30.0	41.0
50-51	36.73504934933899	40.0	37.0	41.0	30.0	41.0
52-53	36.58053433756759	40.0	36.5	41.0	30.0	41.0
54-55	36.4222922541588	40.0	36.0	41.0	30.0	41.0
56-57	36.2626739839081	40.0	36.0	41.0	30.0	41.0
58-59	36.05943895040488	39.0	35.0	41.0	29.0	41.0
60-61	35.83416439215585	39.0	35.0	41.0	29.0	41.0
62-63	35.573665692184306	39.0	35.0	41.0	28.0	41.0
64-65	35.30535194551523	38.5	35.0	40.5	28.0	41.0
66-67	35.01194926655111	38.0	34.5	40.0	28.0	41.0
68-69	34.701824405608875	37.0	34.0	40.0	28.0	41.0
70-71	34.35291552402142	37.0	34.0	39.5	27.5	41.0
72-73	33.98409389310015	36.0	34.0	39.0	26.0	41.0
74-75	33.6129310790883	36.0	34.0	39.0	26.0	40.5
76-77	33.285625630610824	35.0	34.0	38.5	26.0	40.0
78-79	32.91051292913875	35.0	33.0	37.0	26.0	39.0
80-81	32.54963279046905	35.0	33.0	37.0	25.0	39.0
82-83	32.32305868884692	35.0	33.0	36.5	25.0	39.0
84-85	32.04614648280858	35.0	33.0	36.0	25.0	37.0
86-87	31.780435541872556	35.0	33.0	36.0	25.0	37.0
88-89	31.412355121206634	35.0	33.0	35.0	23.0	37.0
90-91	31.20965044757199	35.0	33.0	35.0	21.5	36.0
92-93	31.017218100794246	35.0	33.0	35.0	20.0	36.0
94-95	30.829568694538587	35.0	32.5	35.0	19.5	36.0
96-97	30.638082910770187	35.0	32.0	35.0	16.5	35.5
98-99	30.42661145318604	35.0	32.0	35.0	2.0	35.0
100	30.251784466923652	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2712839126541162
1101	2	0.2295634748570734
1101	3	0.29515831090063926
1101	4	0.20596873350390155
1101	5	0.2260615434961295
1101	6	0.26342455640968154
1101	7	0.29332837106121445
1101	8	0.2884173056353134
1101	9	0.3830515659048004
1101	10-11	0.3531113095024523
1101	12-13	0.3627929128516385
1101	14-15	0.44459233877189064
1101	16-17	0.34310142873808047
1101	18-19	0.3972752680881442
1101	20-21	0.3164615134330546
1101	22-23	0.3541907159739637
1101	24-25	0.3813524023319559
1101	26-27	0.36312893424052817
1101	28-29	0.4011645862908395
1101	30-31	0.45793911341625915
1101	32-33	0.4902814332670147
1101	34-35	0.4699097139816786
1101	36-37	0.45775157824450474
1101	38-39	0.41504947194093234
1101	40-41	0.4297787996597009
1101	42-43	0.39196408367988056
1101	44-45	0.376676423331503
1101	46-47	0.44429542392634147
1101	48-49	0.46768237866142215
1101	50-51	0.3890294717375724
1101	52-53	0.3947471252301469
1101	54-55	0.3281127547308387
1101	56-57	0.2986436657067415
1101	58-59	0.28390200632743756
1101	60-61	0.30874156735639957
1101	62-63	0.2977418669392051
1101	64-65	0.3955706648098385
1101	66-67	0.3129831140092847
1101	68-69	0.38908326478172484
1101	70-71	0.39590106123960567
1101	72-73	0.4114584069738072
1101	74-75	0.38959900035720807
1101	76-77	0.3706556685076663
1101	78-79	0.42564336468728925
1101	80-81	0.460247248307148
1101	82-83	0.42232765947161965
1101	84-85	0.26643849243401974
1101	86-87	0.28158878301213264
1101	88-89	0.4359952861405674
1101	90-91	0.5226500687219868
1101	92-93	0.47696582423202116
1101	94-95	0.5099999838515501
1101	96-97	0.44059696765417655
1101	98-99	0.5041475999424136
1101	100	0.5647003496487919
1102	1	0.20303108262494263
1102	2	0.24633629097705523
1102	3	0.2644022024440389
1102	4	0.22669235219266426
1102	5	0.1682125623880708
1102	6	0.18259724736299177
1102	7	0.20592447780640555
1102	8	0.2770426464612896
1102	9	0.3058307134259195
1102	10-11	0.28042958425557885
1102	12-13	0.3253365963019945
1102	14-15	0.31906958982634137
1102	16-17	0.3613678542401928
1102	18-19	0.24635657142513168
1102	20-21	0.2790888745675275
1102	22-23	0.33516410824835674
1102	24-25	0.3404078045867607
1102	26-27	0.33664749941658556
1102	28-29	0.3331390452692986
1102	30-31	0.3204204601463658
1102	32-33	0.3594787887769044
1102	34-35	0.41346114401265766
1102	36-37	0.3880507342813573
1102	38-39	0.26119981957611316
1102	40-41	0.3959100706181857
1102	42-43	0.39655864235923843
1102	44-45	0.31774746098238893
1102	46-47	0.34619354809519365
1102	48-49	0.316166295598336
1102	50-51	0.3962835068056876
1102	52-53	0.3132061183759802
1102	54-55	0.33400833220750314
1102	56-57	0.2894632372919119
1102	58-59	0.32623723656523396
1102	60-61	0.24069342934546256
1102	62-63	0.3414821252136164
1102	64-65	0.40625310478687027
1102	66-67	0.28389255852424355
1102	68-69	0.3051636093525474
1102	70-71	0.30867467479243516
1102	72-73	0.3164239290723714
1102	74-75	0.31571054425865697
1102	76-77	0.2545036157747802
1102	78-79	0.26960507345786766
1102	80-81	0.18718889892317492
1102	82-83	0.1894854416883689
1102	84-85	0.2393001390399334
1102	86-87	0.25851874800228103
1102	88-89	0.4022003414312145
1102	90-91	0.46974670256777173
1102	92-93	0.49128812876246997
1102	94-95	0.47530379779321663
1102	96-97	0.49891629878787924
1102	98-99	0.55417939918296
1102	100	0.4775129057290428
1103	1	0.22364173579541813
1103	2	0.16463954871020547
1103	3	0.17150157762850782
1103	4	0.12499960057707682
1103	5	0.12053296233683142
1103	6	0.10547722487202549
1103	7	0.058636529787527536
1103	8	0.15358034819676192
1103	9	0.12662101734132136
1103	10-11	0.24292985420977686
1103	12-13	0.19705771055645727
1103	14-15	0.23170185845662417
1103	16-17	0.18641766456885733
1103	18-19	0.17553685417887976
1103	20-21	0.16896462199721896
1103	22-23	0.21854512845378338
1103	24-25	0.17599300018279962
1103	26-27	0.1648077591792898
1103	28-29	0.2143224321804169
1103	30-31	0.22400523766370384
1103	32-33	0.16393728921853068
1103	34-35	0.22148946939371683
1103	36-37	0.2346157985200179
1103	38-39	0.18773696903886616
1103	40-41	0.21084716290835104
1103	42-43	0.18876424431699945
1103	44-45	0.10011469530665096
1103	46-47	0.1889479854966183
1103	48-49	0.19625159398303538
1103	50-51	0.0708360875926104
1103	52-53	0.03773712755518943
1103	54-55	0.09428754261247718
1103	56-57	0.03375467441049551
1103	58-59	-0.004843391900244853
1103	60-61	0.023471543585252164
1103	62-63	0.07517566858496849
1103	64-65	0.10746754426220662
1103	66-67	0.030522829410998042
1103	68-69	-0.007709301813257241
1103	70-71	0.006911457573572477
1103	72-73	0.02350136171968842
1103	74-75	0.14396485524183333
1103	76-77	0.09510858575670511
1103	78-79	0.06453148407917553
1103	80-81	0.1422011750390908
1103	82-83	0.09739693253841608
1103	84-85	0.047127915236607976
1103	86-87	0.1309902387775992
1103	88-89	0.12895769659109746
1103	90-91	0.1596263626161587
1103	92-93	0.09253636952656308
1103	94-95	0.043070683311917435
1103	96-97	0.15196395673664043
1103	98-99	0.1603125883502159
1103	100	0.1959484451437028
1104	1	0.24113897376416915
1104	2	0.23163961059977822
1104	3	0.2431671932875723
1104	4	0.228450529673367
1104	5	0.10287603463556394
1104	6	0.13766717275096596
1104	7	0.17937210826266892
1104	8	0.08521819300113265
1104	9	0.20982783598312693
1104	10-11	0.15020529228759472
1104	12-13	0.18876842824131046
1104	14-15	0.14198265207330252
1104	16-17	0.17848152917119364
1104	18-19	0.1819801388675657
1104	20-21	0.13917258141438538
1104	22-23	0.2040619231092009
1104	24-25	0.24164228556732326
1104	26-27	0.28690459461491713
1104	28-29	0.31238954347626446
1104	30-31	0.2956646591383816
1104	32-33	0.2973330226434072
1104	34-35	0.29531196800957105
1104	36-37	0.3981534001987299
1104	38-39	0.3171266333130234
1104	40-41	0.39800938384073703
1104	42-43	0.36001559187880616
1104	44-45	0.27473499964443704
1104	46-47	0.27942852770781457
1104	48-49	0.21102135222898255
1104	50-51	0.18668976015328553
1104	52-53	0.18690090220856348
1104	54-55	0.21776029719188728
1104	56-57	0.1898818884263136
1104	58-59	0.12922034641392344
1104	60-61	0.20549983109241765
1104	62-63	0.12268927070024915
1104	64-65	0.13955172056218146
1104	66-67	0.19040021696851284
1104	68-69	0.14832552215272443
1104	70-71	0.24293970368163542
1104	72-73	0.22406424319781593
1104	74-75	0.22406206264215456
1104	76-77	0.1166596691169346
1104	78-79	0.16102443619504925
1104	80-81	0.14294973046120418
1104	82-83	0.20927516710492
1104	84-85	0.24036770262083706
1104	86-87	0.31529699297897906
1104	88-89	0.33823417508279974
1104	90-91	0.25112810890571424
1104	92-93	0.2222127957884794
1104	94-95	0.22666230977824142
1104	96-97	0.2799751405587081
1104	98-99	0.27301582555120163
1104	100	0.3510904440765259
1105	1	0.15086324135958407
1105	2	0.15060432847456084
1105	3	0.19557578065294834
1105	4	0.20646926233676766
1105	5	0.10035939863956855
1105	6	0.15766435642093768
1105	7	0.14924938454801406
1105	8	0.1133972718964813
1105	9	0.16891905451128508
1105	10-11	0.14414336021771135
1105	12-13	0.14238030700138182
1105	14-15	0.22072931872144608
1105	16-17	0.2793827051626465
1105	18-19	0.1863140668917751
1105	20-21	0.23665635228778115
1105	22-23	0.2912492687965411
1105	24-25	0.2677671950636977
1105	26-27	0.23703272870001513
1105	28-29	0.2385081663444666
1105	30-31	0.28323358380443153
1105	32-33	0.3067083600472884
1105	34-35	0.27912923443478377
1105	36-37	0.2778981422554523
1105	38-39	0.2297210667047338
1105	40-41	0.2439831234983174
1105	42-43	0.2524809811873041
1105	44-45	0.21516349165550963
1105	46-47	0.1983266331254825
1105	48-49	0.21992157039036897
1105	50-51	0.25162983523954097
1105	52-53	0.17007266043209057
1105	54-55	0.24777150396251102
1105	56-57	0.24218335998355656
1105	58-59	0.16629671599580576
1105	60-61	0.1606530749727071
1105	62-63	0.2307521665543959
1105	64-65	0.2098820459068449
1105	66-67	0.19946477066653756
1105	68-69	0.07155098118078485
1105	70-71	0.10428876446832192
1105	72-73	0.1063536555434581
1105	74-75	0.06518728374092575
1105	76-77	0.08267964997621391
1105	78-79	0.1551508300700135
1105	80-81	0.15196280525848493
1105	82-83	0.1334214906331681
1105	84-85	0.14455656186228083
1105	86-87	0.23296451698228537
1105	88-89	0.261361092330727
1105	90-91	0.226554488173079
1105	92-93	0.2933520221253545
1105	94-95	0.3205117694953934
1105	96-97	0.3680003642773251
1105	98-99	0.32812918630819254
1105	100	0.3461555153917999
1106	1	0.13637278671381026
1106	2	0.09009069220025268
1106	3	0.14932018185562157
1106	4	0.07442409328822919
1106	5	0.1060034984179623
1106	6	0.075476685414813
1106	7	0.03258149280982536
1106	8	0.10202734841652727
1106	9	0.030205780203097277
1106	10-11	0.03880845085662088
1106	12-13	0.01846947904450502
1106	14-15	0.0694458185264395
1106	16-17	0.03687024627970459
1106	18-19	0.01075034188323798
1106	20-21	-0.01609729515011793
1106	22-23	0.02626152644023705
1106	24-25	0.011002225697410495
1106	26-27	0.11061557446118542
1106	28-29	0.13219927788052388
1106	30-31	0.12697101816075929
1106	32-33	0.09702135416997493
1106	34-35	0.06455872813571517
1106	36-37	0.020987581483844053
1106	38-39	0.006529135284054632
1106	40-41	0.030516220836901198
1106	42-43	-0.01107392349520353
1106	44-45	0.020585066166567856
1106	46-47	0.11310858708497307
1106	48-49	0.010345505919737263
1106	50-51	0.033789734283573125
1106	52-53	-0.00443951860547287
1106	54-55	0.05755361664591874
1106	56-57	0.011336967513727814
1106	58-59	0.06381000345540855
1106	60-61	-0.01804145574112681
1106	62-63	0.040878606218136326
1106	64-65	0.04524428979446071
1106	66-67	0.09792676422385682
1106	68-69	0.010140436659476393
1106	70-71	0.06263086855795308
1106	72-73	0.16951302116174816
1106	74-75	0.15209266897900164
1106	76-77	0.11062186237602845
1106	78-79	0.1113568928324753
1106	80-81	0.11958383413716689
1106	82-83	-0.020105284013638425
1106	84-85	0.0432050560257764
1106	86-87	0.08303699618189953
1106	88-89	0.12567750808762668
1106	90-91	0.09511832768959039
1106	92-93	0.0808756086888458
1106	94-95	0.1040991445569901
1106	96-97	0.05931339940368474
1106	98-99	0.10559565210092359
1106	100	0.13420059978757592
1107	1	0.08147413806516113
1107	2	0.09925396506215378
1107	3	0.10241914983044609
1107	4	0.05613804182581106
1107	5	0.0015398738344742924
1107	6	0.06868232684391273
1107	7	0.03705032379834705
1107	8	0.022048289734556192
1107	9	0.041127660987989145
1107	10-11	0.020288111167623413
1107	12-13	0.05920670599748945
1107	14-15	0.02414575604957747
1107	16-17	-0.015759277507783054
1107	18-19	0.07653037705539845
1107	20-21	0.037872877933935456
1107	22-23	0.05889266999817977
1107	24-25	0.031324560018035186
1107	26-27	0.09591760380216385
1107	28-29	0.09230809174575683
1107	30-31	0.07752607878644824
1107	32-33	0.09914874911628857
1107	34-35	0.05877383365218236
1107	36-37	0.03986224595995935
1107	38-39	0.07830232114114466
1107	40-41	0.056254662617718054
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2216	52-53	-0.25045061488284404
2216	54-55	-0.29709929818265834
2216	56-57	-0.4207504531580497
2216	58-59	-0.41758043330128913
2216	60-61	-0.296704380114889
2216	62-63	-0.22487356885861232
2216	64-65	-0.23821085751095694
2216	66-67	-0.2622371646516086
2216	68-69	-0.31140495617647446
2216	70-71	-0.38179317950334024
2216	72-73	-0.2352074919564373
2216	74-75	-0.23063405334640663
2216	76-77	-0.27812513863008803
2216	78-79	-0.3162012408904076
2216	80-81	-0.3557182419405365
2216	82-83	-0.32033677893147683
2216	84-85	-0.3122839895847278
2216	86-87	-0.33203984094371464
2216	88-89	-0.32822803650386234
2216	90-91	-0.3188313472581683
2216	92-93	-0.2653066801561863
2216	94-95	-0.23943620971125057
2216	96-97	-0.28305879669826695
2216	98-99	-0.3299134835438977
2216	100	-0.39067766685845484
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56520.0
3	8886.0
4	5547.0
5	4326.0
6	3754.0
7	3799.0
8	4529.0
9	5095.0
10	5604.0
11	6035.0
12	6570.0
13	7160.0
14	7775.0
15	7992.0
16	7908.0
17	8124.0
18	8110.0
19	8089.0
20	8162.0
21	8544.0
22	9717.0
23	12898.0
24	18152.0
25	23396.0
26	24798.0
27	23450.0
28	25494.0
29	31506.0
30	37937.0
31	46580.0
32	60190.0
33	80644.0
34	111292.0
35	166771.0
36	302947.0
37	619388.0
38	943338.0
39	370675.0
40	538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07016467995554	19.772302073538487	13.221930850901597	23.93560239560438
2	15.255790280720477	19.896918498334536	40.720819368106966	24.126471852838026
3	17.995373131478722	25.511526559189647	30.104675700138277	26.38842460919335
4	11.85217512833885	16.66774505376191	39.40779078600185	32.07228903189739
5	12.559379934847476	38.340988598470155	36.28576263037865	12.813868836303719
6	30.824162940562914	38.58576776603572	17.79629195404158	12.793777339359785
7	27.206589107674873	32.50229947361136	22.950072638786654	17.341038779927114
8	25.05754829544811	37.04577115039342	20.27863822151266	17.618042332645807
9	24.908164489794995	16.731448288933905	20.998043451611412	37.36234376965969
10-11	23.730353549582432	27.09334908359423	29.27934469944097	19.896952667382372
12-13	24.239788675564142	24.400491115194395	29.193101358167684	22.16661885107378
14-15	21.514899174861956	26.442536051372972	25.999586953888432	26.04297781987664
16-17	21.47048753660887	29.80027231550172	26.648589928725404	22.080650219164006
18-19	21.404856549413346	28.9276773698874	28.39926841405668	21.268197666642568
20-21	22.486317706106966	28.235056463772956	27.890288049909824	21.388337780210257
22-23	22.067019184725574	27.979046862196295	27.967823468633448	21.986110484444684
24-25	22.02726412292954	28.117198270591715	27.87849244144033	21.97704516503842
26-27	21.71363104429567	28.075218476701963	28.03315334221321	22.177997136789156
28-29	21.80780489382692	28.092167612763415	28.126744618569372	21.973282874840297
30-31	22.02054782335587	28.49573540318869	27.694164574719316	21.789552198736132
32-33	21.70466043360574	28.173892566389085	28.22095752444143	21.900489475563745
34-35	21.816644891082458	27.994344978621438	28.22055877135572	21.968451358940385
36-37	22.0300032568644	28.28735505417165	27.737035528929983	21.94560616003397
38-39	21.911451616163657	28.467622147203297	27.871612525015664	21.749313711617376
40-41	21.751014820207594	28.17707209775651	28.15991451285881	21.911998569177086
42-43	21.645459801016344	28.103898414033157	28.131081796755296	22.1195599881952
44-45	21.808401569755745	28.178790962067257	28.11759972522159	21.895207742955407
46-47	21.867822685742404	28.319427242742172	27.908041761447844	21.904708310067576
48-49	21.841769731485726	28.237795742871164	27.92912277467714	21.991311750965966
50-51	21.80793907700577	28.149152281450935	27.98697750623416	22.05593113530913
52-53	21.880312607080892	28.068062172774127	28.01372834671708	22.037896873427897
54-55	21.858895115264303	28.53535545452855	27.831569313410732	21.77418011679642
56-57	21.756786671261104	28.24611584416063	27.891863432687757	22.105234051890505
58-59	21.654791135919513	27.83704179222012	28.23574592697458	22.272421144885787
60-61	21.8167118082225	28.251477543760362	27.96114322674536	21.970667421271777
62-63	21.75929227293932	28.273734882092654	27.910783831004455	22.05618901396357
64-65	21.691447731284683	28.033948223251592	28.033851204304277	22.24075284115945
66-67	21.586510910720826	28.04907075507699	27.9609939628509	22.40342437135129
68-69	21.917007030860532	28.137380631330238	27.928448571521436	22.017163766287798
70-71	21.845816966081	27.983728607900815	28.019674174379816	22.15078025163837
72-73	21.67492862249047	28.129295606618683	28.058488082296485	22.137287688594363
74-75	21.67366147910571	28.174075023304813	27.92965140204824	22.22261209554124
76-77	21.759786735245314	28.108313425194826	27.829847428252275	22.302052411307585
78-79	21.83017001044748	28.203132238630708	27.9386546538175	22.028043097104312
80-81	21.78642790579896	27.970673304159284	28.0417084460994	22.201190343942358
82-83	21.665952768220052	28.009267905632168	28.21428531003971	22.11049401610807
84-85	21.601025949166296	27.97176473023235	28.066714508073805	22.36049481252755
86-87	21.83227870769781	28.052519079229597	27.962275054924994	22.1529271581476
88-89	21.625590047844774	27.9470019623661	28.076817775200986	22.35059021458814
90-91	21.55422502775187	27.85662838918117	28.15014822418141	22.438998358885552
92-93	21.77473252244625	27.98676489632459	28.02361403319253	22.214888548036633
94-95	21.584043936044697	27.74863395267102	28.170091131635864	22.49723097964841
96-97	21.55601358382213	27.769390593770876	28.203592064359352	22.471003758047644
98-99	21.507416061504877	27.868954854618842	28.12034539156658	22.503283692309694
100	21.71175784343132	27.66199087397352	27.985787186475836	22.640464096119324
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	329.0
1	299.5
2	244.5
3	200.5
4	179.5
5	163.0
6	139.0
7	135.0
8	155.5
9	158.0
10	161.5
11	177.0
12	203.0
13	236.5
14	274.0
15	330.0
16	413.0
17	545.5
18	751.5
19	1124.0
20	1699.0
21	2583.5
22	3881.0
23	5931.5
24	8929.5
25	12739.5
26	17944.5
27	24795.5
28	33545.0
29	42977.5
30	51790.0
31	60980.0
32	69976.0
33	78744.0
34	86848.0
35	93243.0
36	98412.5
37	103420.5
38	107171.5
39	109027.5
40	110343.5
41	111922.5
42	113721.5
43	113995.5
44	112253.5
45	110317.5
46	108172.5
47	104618.5
48	101807.5
49	99694.0
50	98107.0
51	95429.5
52	88393.5
53	82496.5
54	79651.0
55	75833.5
56	69150.5
57	62289.5
58	55550.5
59	48687.0
60	43042.5
61	37070.0
62	31673.0
63	26582.5
64	21656.5
65	17223.5
66	13252.5
67	9878.0
68	7537.5
69	5956.5
70	4413.0
71	3119.5
72	2355.5
73	1752.0
74	1248.5
75	855.0
76	497.5
77	304.5
78	213.5
79	145.5
80	95.5
81	61.0
82	39.0
83	24.5
84	19.0
85	18.0
86	17.0
87	11.0
88	4.5
89	4.0
90	6.5
91	6.0
92	2.5
93	3.5
94	3.5
95	2.0
96	1.5
97	1.0
98	0.5
99	1.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33186298605541614
2	0.11693788321734407
3	0.06561586422787365
4	0.038936175717279385
5	0.043301942928103895
6	0.010186790158590536
7	0.007373295733836959
8	0.01581377900809769
9	0.01846557835096888
10-11	0.02105269966108711
12-13	0.016201847204615424
14-15	0.018611103924663027
16-17	0.01958127441595736
18-19	0.019419579334074975
20-21	0.024076397692287792
22-23	0.01563591441802706
24-25	0.0251597547408998
26-27	0.019597443924145603
28-29	0.019080019662121958
30-31	0.020147207202545727
32-33	0.024836364577135023
34-35	0.01534486327063876
36-37	0.009992756060331669
38-39	0.0138249295009443
40-41	0.01012211212583758
42-43	0.008974077044472615
44-45	0.0095076708146845
46-47	0.008068584585931234
48-49	0.004284919669883321
50-51	0.006645667865366207
52-53	0.007874550487672367
54-55	0.003476444260471374
56-57	0.0030560370475771607
58-59	0.0031530540967065947
60-61	0.0037189868832949577
62-63	0.0028296639329418154
64-65	0.0019565104907769124
66-67	0.0016816288515768504
68-69	0.002797324916565338
70-71	0.002085866556282824
72-73	0.001762476392518045
74-75	0.0021828836054122577
76-77	0.003913020981553825
78-79	0.0034441052440948956
80-81	0.0035087832768478516
82-83	0.002053527539906346
84-85	0.0025547822937417534
86-87	0.0022152226217887357
88-89	0.6032681809950069
90-91	0.6152012780379271
92-93	0.6111427314826792
94-95	0.5982556334566528
96-97	0.5962182754249347
98-99	0.5930167128036633
100	0.5948438672289343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3092240.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.75674248595131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.87024684325043	46.85499296644176
2	14.109992216971445	17.427743116445658
3	4.646472105251161	8.608529438164558
4	1.9992022851635427	4.938568828086812
5	1.0214128155397906	3.153956411057067
6	0.614341858989988	2.2763851190391184
7	0.37969722905836656	1.641420479831077
8	0.25575617744139606	1.2635734711551654
9	0.1940627065262211	1.078621253375992
>10	0.8469328306993368	9.19147584118342
>50	0.044538815865768216	1.872980031679881
>100	0.017239427085983115	1.6583926139448197
>500	1.0468815668771085E-4	0.03336042959462916
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.2339016376477895E-5	0.0
5	0.0	0.0	0.0	3.2339016376477895E-5	0.0
6	0.0	0.0	0.0	3.2339016376477895E-5	0.0
7	0.0	0.0	0.0	9.701704912943369E-5	0.0
8	0.0	0.0	0.0	9.701704912943369E-5	0.0
9	0.0	0.0	0.0	1.2935606550591158E-4	0.0
10-11	0.0	0.0	0.0	1.2935606550591158E-4	0.0
12-13	0.0	0.0	0.0	1.2935606550591158E-4	0.0
14-15	0.0	0.0	0.0	1.9403409825886737E-4	0.0
16-17	0.0	0.0	0.0	1.9403409825886737E-4	0.0
18-19	0.0	0.0	0.0	2.2637311463534525E-4	0.0
20-21	0.0	0.0	0.0	2.5871213101182316E-4	0.0
22-23	0.0	0.0	0.0	2.9105114738830107E-4	0.0
24-25	0.0	0.0	0.0	3.5572918014125683E-4	0.0
26-27	0.0	0.0	0.0	4.6891573745892946E-4	0.0
28-29	0.0	0.0	0.0	0.0010186790158590536	0.0
30-31	0.0	0.0	0.0	0.0038160039324243913	0.0
32-33	0.0	0.0	0.0	0.010865909502496572	0.0
34-35	0.0	0.0	0.0	0.020680800972757613	0.0
36-37	0.0	0.0	0.0	0.04003570227407963	0.0
38-39	0.0	0.0	0.0	0.0747354668460404	0.0
40-41	0.0	0.0	0.0	0.11554730551315551	0.0
42-43	0.0	0.0	0.0	0.1557608723773058	0.0
44-45	0.0	0.0	0.0	0.20124569891082195	0.0
46-47	0.0	0.0	0.0	0.25788748609422296	0.0
48-49	0.0	0.0	0.0	0.32243616278167286	0.0
50-51	0.0	0.0	0.0	0.3948432204486068	0.0
52-53	0.0	0.0	0.0	0.4656494968049052	0.0
54-55	0.0	0.0	0.0	0.5442171370915583	0.0
56-57	0.0	0.0	0.0	0.6339093990117197	0.0
58-59	0.0	0.0	0.0	0.7384614389568727	0.0
60-61	0.0	0.0	0.0	0.8535559982407575	0.0
62-63	0.0	0.0	0.0	0.9685212014591364	0.0
64-65	0.0	0.0	0.0	1.0885151217240576	0.0
66-67	0.0	0.0	0.0	1.2265380436188653	0.0
68-69	0.0	0.0	0.0	1.388782888779655	0.0
70-71	0.0	0.0	0.0	1.5652245621297183	0.0
72-73	0.0	0.0	0.0	1.7434125423641116	0.0
74-75	0.0	0.0	0.0	1.926774765218741	0.0
76-77	0.0	0.0	0.0	2.121293948723256	0.0
78-79	0.0	0.0	0.0	2.3449344164747883	0.0
80-81	0.0	0.0	0.0	2.591147517657103	0.0
82-83	0.0	0.0	0.0	2.847337205391561	0.0
84-85	0.0	0.0	0.0	3.101990789848136	0.0
86-87	0.0	0.0	0.0	3.3732342897058443	0.0
88	0.0	0.0	0.0	3.5977802499159184	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2495	0.0	32.295006	1
GTATCAA	3360	0.0	30.992945	1
TCAACGC	3245	0.0	29.96501	4
ATCAACG	3350	0.0	29.032852	3
GTACATG	8125	0.0	28.475277	1
CAACGCA	3540	0.0	27.732416	5
TACATGG	8210	0.0	27.085396	2
TACACCG	410	0.0	26.35049	5
AACGCAG	3740	0.0	25.863829	6
ACATGGG	8335	0.0	25.705343	3
TATCAAC	4175	0.0	23.985037	2
ACACCGT	590	0.0	22.284517	6
ACGCAGA	4380	0.0	21.977432	7
GTCGTAC	310	1.2732926E-11	21.280224	1
CGCAGAG	4555	0.0	20.926897	8
CATGGGG	5785	0.0	18.675974	4
GTGTAGG	1140	0.0	18.186855	1
GTACCGT	260	3.7061545E-7	18.060255	6
CGTACAC	375	2.0190782E-10	17.541265	3
GAGTACA	5645	0.0	17.278906	1
>>END_MODULE