FastQCFastQC Report
Wed 25 May 2016
SRR1033145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3092240
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41990.13579152976483067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35210.11386567666157865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40550.051.764881
GTATCAA70000.040.811441
TCAACGC79350.035.3123474
ATCAACG79700.035.1572763
CAACGCA82000.034.2284935
AACGCAG83500.033.7114946
TATCAAC86700.032.5893482
ACGCAGA97200.028.8632837
CGCAGAG99100.028.2146028
GTACATG83200.027.8878351
TACATGG85500.026.1734582
ACATGGG85250.025.037373
GCAGAGT111500.024.6553759
TAGTGCG1100.003063449921.3603299
GAGTACT72150.021.26524712-13
GTGGTAT18000.020.917451
CAGAGTA114900.019.87649710-11
AGAGTAC103000.019.6864410-11
TACACCG5250.018.8056035
GAGTACA59000.018.1077751
Produced by FastQC (version 0.11.5)