##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033145_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3092240 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75262431117895 34.0 31.0 34.0 31.0 34.0 2 32.83148267922283 34.0 31.0 34.0 31.0 34.0 3 32.93365133366103 34.0 31.0 34.0 31.0 34.0 4 36.3649820195069 37.0 37.0 37.0 35.0 37.0 5 36.30747613380591 37.0 37.0 37.0 35.0 37.0 6 36.3900221198872 37.0 37.0 37.0 35.0 37.0 7 36.35394503660777 37.0 37.0 37.0 35.0 37.0 8 36.34635118878224 37.0 37.0 37.0 35.0 37.0 9 38.162255840426354 39.0 39.0 39.0 37.0 39.0 10-11 38.14592690088739 39.0 39.0 39.0 37.0 39.0 12-13 38.12114535094301 39.0 39.0 39.0 37.0 39.0 14-15 39.659288573978735 41.0 40.0 41.0 37.0 41.0 16-17 39.66795591545288 41.0 40.0 41.0 37.0 41.0 18-19 39.67457522701989 41.0 40.0 41.0 37.0 41.0 20-21 39.665243965539545 41.0 40.0 41.0 37.0 41.0 22-23 39.60625323390164 41.0 40.0 41.0 37.0 41.0 24-25 39.56644406643727 41.0 40.0 41.0 37.0 41.0 26-27 39.47386635578093 41.0 39.0 41.0 36.5 41.0 28-29 39.3865602281841 41.0 39.0 41.0 36.0 41.0 30-31 39.30796606990402 41.0 39.0 41.0 36.0 41.0 32-33 39.356475079553974 41.0 39.0 41.0 36.0 41.0 34-35 39.31560826455903 41.0 39.0 41.0 36.0 41.0 36-37 39.24357294388534 41.0 39.0 41.0 35.0 41.0 38-39 39.163108296898045 41.0 39.0 41.0 35.0 41.0 40-41 39.06188523529869 41.0 39.0 41.0 35.0 41.0 42-43 38.962956626911236 41.0 39.0 41.0 35.0 41.0 44-45 38.84140089385041 41.0 39.0 41.0 35.0 41.0 46-47 38.66328777843893 41.0 39.0 41.0 35.0 41.0 48-49 38.50763006752386 41.0 39.0 41.0 35.0 41.0 50-51 38.38860082011745 41.0 38.0 41.0 35.0 41.0 52-53 38.260440004656814 40.5 38.0 41.0 34.0 41.0 54-55 38.130242963030035 40.0 38.0 41.0 34.0 41.0 56-57 37.977613800998625 40.0 37.5 41.0 34.0 41.0 58-59 37.79057075130003 40.0 37.0 41.0 34.0 41.0 60-61 37.59917600186273 40.0 36.5 41.0 33.5 41.0 62-63 37.367549575712104 39.5 36.0 41.0 33.0 41.0 64-65 37.11731139885649 39.0 35.5 41.0 33.0 41.0 66-67 36.836490860993976 39.0 35.0 41.0 33.0 41.0 68-69 36.51161860010866 38.5 35.0 41.0 32.5 41.0 70-71 36.17275761260446 37.0 35.0 40.0 32.0 41.0 72-73 35.817019377538614 37.0 35.0 39.5 32.0 41.0 74-75 35.47661775929423 36.0 35.0 39.0 31.5 41.0 76-77 35.13221952371097 36.0 35.0 39.0 31.0 40.5 78-79 34.78395305668383 35.5 35.0 37.5 31.0 39.5 80-81 34.43953590277599 35.0 35.0 37.0 31.0 39.0 82-83 34.11677036711251 35.0 34.5 37.0 31.0 39.0 84-85 33.841021395493236 35.0 34.0 36.0 31.0 37.5 86-87 33.605962506144415 35.0 34.0 36.0 30.5 37.0 88-89 33.38838592735364 35.0 34.0 36.0 30.0 37.0 90-91 33.18916416578273 35.0 34.0 35.0 30.0 36.0 92-93 33.02712240964479 35.0 34.0 35.0 30.0 36.0 94-95 32.88056214912167 35.0 34.0 35.0 30.0 36.0 96-97 32.759903662070215 35.0 34.0 35.0 30.0 36.0 98-99 32.65114172897317 35.0 34.0 35.0 30.0 36.0 100 32.557158564665095 35.0 34.0 35.0 30.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11558751208946205 1101 2 0.16566789542125093 1101 3 0.16452907153458796 1101 4 0.11475866416240166 1101 5 0.044481851309065235 1101 6 0.08637705370752968 1101 7 0.0897035756771487 1101 8 0.14185161070692942 1101 9 0.18657820918917167 1101 10-11 0.20757321220117575 1101 12-13 0.19444591743616968 1101 14-15 0.18147823576691735 1101 16-17 0.22191571166399626 1101 18-19 0.21533675774908545 1101 20-21 0.24629545252116714 1101 22-23 0.2698323201255448 1101 24-25 0.25747602466723407 1101 26-27 0.2703332191208716 1101 28-29 0.20878662920100766 1101 30-31 0.2576523257278325 1101 32-33 0.21167490224409136 1101 34-35 0.2204422534828936 1101 36-37 0.34477419552582944 1101 38-39 0.2745367560192733 1101 40-41 0.30603909188432254 1101 42-43 0.2346557850753257 1101 44-45 0.23622327195367632 1101 46-47 0.268442068470641 1101 48-49 0.28165519835324204 1101 50-51 0.2640453742854092 1101 52-53 0.31174175797902137 1101 54-55 0.31991553512833093 1101 56-57 0.33380095639729035 1101 58-59 0.2891333959971547 1101 60-61 0.3441280246284677 1101 62-63 0.29786083952238585 1101 64-65 0.3361582180278688 1101 66-67 0.3029306547511297 1101 68-69 0.3199469763043581 1101 70-71 0.3389399685597141 1101 72-73 0.38511169828444736 1101 74-75 0.4019929756626368 1101 76-77 0.3596838028651632 1101 78-79 0.36169210408004204 1101 80-81 0.2913594440963436 1101 82-83 0.3601664185595226 1101 84-85 0.3924308271937704 1101 86-87 0.3281255631577622 1101 88-89 0.37529420191983576 1101 90-91 0.410651495571571 1101 92-93 0.4114055717905103 1101 94-95 0.43004695488303923 1101 96-97 0.43708751008307445 1101 98-99 0.4516960172821385 1101 100 0.504406713866878 1102 1 0.10456086949336907 1102 2 0.11840067151129574 1102 3 0.08626267815002109 1102 4 0.061860488433417515 1102 5 0.04951805495520034 1102 6 0.05960994476119197 1102 7 0.08826769185297678 1102 8 0.12128594153293903 1102 9 0.17541007732185676 1102 10-11 0.14209949642851427 1102 12-13 0.14934659936628236 1102 14-15 0.19575104486118988 1102 16-17 0.15412634932867064 1102 18-19 0.15020864973823222 1102 20-21 0.18675267220480407 1102 22-23 0.19305093320836164 1102 24-25 0.19746114506587986 1102 26-27 0.27135882994072347 1102 28-29 0.2130459609690334 1102 30-31 0.29287136826815896 1102 32-33 0.20323822448558815 1102 34-35 0.2783043396977547 1102 36-37 0.22543324303060785 1102 38-39 0.16389421062556409 1102 40-41 0.179490330649557 1102 42-43 0.17237208744392518 1102 44-45 0.2605444862080617 1102 46-47 0.29286935666386427 1102 48-49 0.2961150348141288 1102 50-51 0.274545776586379 1102 52-53 0.3187147358223328 1102 54-55 0.21846328282502725 1102 56-57 0.2537656603586669 1102 58-59 0.3095121238336844 1102 60-61 0.3368697741321256 1102 62-63 0.39324901192548367 1102 64-65 0.35595097266453735 1102 66-67 0.3291915395173177 1102 68-69 0.391514681444157 1102 70-71 0.36678281464249807 1102 72-73 0.3156819321650133 1102 74-75 0.31097691923989856 1102 76-77 0.2778227389059964 1102 78-79 0.2819746857413534 1102 80-81 0.3214845861089657 1102 82-83 0.24465883884678163 1102 84-85 0.2307165622247993 1102 86-87 0.2772562128137537 1102 88-89 0.30487349472698355 1102 90-91 0.2794612324193011 1102 92-93 0.2488731122762573 1102 94-95 0.23179089187850366 1102 96-97 0.2820424540376649 1102 98-99 0.34366666459583683 1102 100 0.3217499147051939 1103 1 0.09508980443514758 1103 2 0.12251139400436273 1103 3 0.09736473369810739 1103 4 0.05057445188263188 1103 5 0.028915644661189788 1103 6 0.08448469287949933 1103 7 0.10924757093656723 1103 8 0.08790558900049916 1103 9 0.08570224827625594 1103 10-11 0.09404047677359273 1103 12-13 0.09820669472756549 1103 14-15 0.1412970642634903 1103 16-17 0.15266749757564924 1103 18-19 0.12135171304720416 1103 20-21 0.1341617190694535 1103 22-23 0.1234812207017626 1103 24-25 0.13802995080112623 1103 26-27 0.17277891819335167 1103 28-29 0.1546318277550256 1103 30-31 0.16502506867949762 1103 32-33 0.17306822504995267 1103 34-35 0.1767771599940886 1103 36-37 0.16701916476910839 1103 38-39 0.1907123817280052 1103 40-41 0.23456098831185557 1103 42-43 0.13324381229494975 1103 44-45 0.20429105820180382 1103 46-47 0.26350312011232546 1103 48-49 0.15889795849756894 1103 50-51 0.16445309423292542 1103 52-53 0.1587550962426576 1103 54-55 0.15046444276259763 1103 56-57 0.18889114523900474 1103 58-59 0.20776760695389385 1103 60-61 0.1945587100652446 1103 62-63 0.15717627216755403 1103 64-65 0.15243512208019894 1103 66-67 0.15960196717369257 1103 68-69 0.21450539375562272 1103 70-71 0.226512741740855 1103 72-73 0.26467179787810835 1103 74-75 0.2206799443370997 1103 76-77 0.16905424718038375 1103 78-79 0.13583301959993577 1103 80-81 0.184318176006407 1103 82-83 0.1737652379511445 1103 84-85 0.1420168644471147 1103 86-87 0.16342585307979363 1103 88-89 0.15124270822082764 1103 90-91 0.12004913964693742 1103 92-93 0.09896983693632677 1103 94-95 0.1305545483327819 1103 96-97 0.2631444233103082 1103 98-99 0.2499818399896867 1103 100 0.24311913781573224 1104 1 0.09640469648802252 1104 2 0.15623985183331257 1104 3 0.13235638696930607 1104 4 0.07294910040840819 1104 5 0.042235231958102304 1104 6 0.04494211745367238 1104 7 0.09962714010151075 1104 8 0.09384359028512534 1104 9 0.14708645874095083 1104 10-11 0.1299084633887233 1104 12-13 0.1852383568891227 1104 14-15 0.21784178930651876 1104 16-17 0.21377090501476914 1104 18-19 0.1790412885432744 1104 20-21 0.19492478613265973 1104 22-23 0.1880194732664151 1104 24-25 0.15512704968561053 1104 26-27 0.1556268557233551 1104 28-29 0.1839224268962667 1104 30-31 0.15024827990358602 1104 32-33 0.12470638557477542 1104 34-35 0.15268112933198807 1104 36-37 0.15806796720588778 1104 38-39 0.18201906609452578 1104 40-41 0.18314648750143192 1104 42-43 0.16452559342665296 1104 44-45 0.17799444552682075 1104 46-47 0.18928505617704872 1104 48-49 0.12394124878976953 1104 50-51 0.2235393145614779 1104 52-53 0.2013118872432429 1104 54-55 0.16494814468737218 1104 56-57 0.20635594868106466 1104 58-59 0.27541369352569944 1104 60-61 0.2685362201055739 1104 62-63 0.2257621759393018 1104 64-65 0.19646419491585476 1104 66-67 0.24600702355174775 1104 68-69 0.2225887521096297 1104 70-71 0.1874134556036111 1104 72-73 0.20658273669274507 1104 74-75 0.20031176724426558 1104 76-77 0.14283596349531535 1104 78-79 0.19760610461948858 1104 80-81 0.18808677528647877 1104 82-83 0.21939322056854138 1104 84-85 0.23530470511157375 1104 86-87 0.2194635938009455 1104 88-89 0.2094880092132385 1104 90-91 0.28377612132594976 1104 92-93 0.2307244251281304 1104 94-95 0.16723507080622113 1104 96-97 0.24309925855859404 1104 98-99 0.3171695569168165 1104 100 0.2972912790611204 1105 1 0.07139091254718011 1105 2 0.04391085605840317 1105 3 0.060960733683941726 1105 4 0.07203681985986066 1105 5 0.048467230468759226 1105 6 0.06420018815447293 1105 7 -0.017699650639805498 1105 8 0.028620592122187816 1105 9 0.052825936900198656 1105 10-11 0.05539346161834402 1105 12-13 0.09129943552565578 1105 14-15 0.11060121617244789 1105 16-17 0.130204299901159 1105 18-19 0.0855773623767746 1105 20-21 0.13442077817151699 1105 22-23 0.17346900538647247 1105 24-25 0.06259949371229112 1105 26-27 0.14939691636872965 1105 28-29 0.12326207193150651 1105 30-31 0.16547886469199113 1105 32-33 0.11869885988048168 1105 34-35 0.050018184097801566 1105 36-37 0.05224004953561234 1105 38-39 0.01778482503890899 1105 40-41 0.11234233298792162 1105 42-43 0.06332263943584593 1105 44-45 0.10247847064395899 1105 46-47 0.18103262865537317 1105 48-49 0.11739008109447724 1105 50-51 0.10109750581469967 1105 52-53 0.11962300914686352 1105 54-55 0.16639687259535663 1105 56-57 0.12515139400655784 1105 58-59 0.11256427211381492 1105 60-61 0.06607188667273789 1105 62-63 0.05824047987747605 1105 64-65 -0.019814396093480013 1105 66-67 0.030183357170351144 1105 68-69 0.017172581294353506 1105 70-71 0.152274900700462 1105 72-73 0.046714576266325025 1105 74-75 -0.016009197722887336 1105 76-77 -0.07447724177802684 1105 78-79 0.023322884428914392 1105 80-81 0.04030787029306282 1105 82-83 0.0767709136469179 1105 84-85 0.0634900904823823 1105 86-87 0.07942756204370482 1105 88-89 0.07186027207388435 1105 90-91 0.06973325277373732 1105 92-93 0.15116463993928875 1105 94-95 0.11057749188150012 1105 96-97 0.1488043512930517 1105 98-99 0.041883617052512534 1105 100 0.03383715050785696 1106 1 0.036574820662657714 1106 2 0.033829903079983126 1106 3 0.03399911388366661 1106 4 0.00976078120076096 1106 5 -0.0109162537026819 1106 6 0.005524678801855032 1106 7 0.021032481581755746 1106 8 0.06339123983671868 1106 9 0.09718870571049365 1106 10-11 0.06278194200309173 1106 12-13 0.08155931382220416 1106 14-15 0.09652129433263923 1106 16-17 0.09777371988616324 1106 18-19 0.07734559030734545 1106 20-21 0.04722565092028219 1106 22-23 0.12402657511918136 1106 24-25 0.10059353608553323 1106 26-27 0.03926318296293374 1106 28-29 0.025779934041239017 1106 30-31 0.10856878103692935 1106 32-33 0.07135779732209357 1106 34-35 0.1274458381129122 1106 36-37 0.14993979508605548 1106 38-39 0.13763633597725544 1106 40-41 0.1687264310808132 1106 42-43 0.20108966408793805 1106 44-45 0.16557236264517883 1106 46-47 0.19188126687972584 1106 48-49 0.1663044472720756 1106 50-51 0.1594763526022831 1106 52-53 0.13342698283800303 1106 54-55 0.08153756151007485 1106 56-57 0.136030359837072 1106 58-59 0.14754723669116743 1106 60-61 0.1552337969755797 1106 62-63 0.12743185047364847 1106 64-65 0.10386909291526791 1106 66-67 0.12824977387203518 1106 68-69 0.18620715533226218 1106 70-71 0.15090097038631 1106 72-73 0.1434567819770649 1106 74-75 0.13201417928723203 1106 76-77 0.13359677950004567 1106 78-79 0.1363949707868244 1106 80-81 0.12195500604433818 1106 82-83 0.13284285469685386 1106 84-85 0.14750088994453137 1106 86-87 0.1536621253866386 1106 88-89 0.09617788676325034 1106 90-91 0.09570098212224565 1106 92-93 0.03387437092492007 1106 94-95 0.01867438168628155 1106 96-97 0.038074836315566074 1106 98-99 0.011739842469197015 1106 100 0.06151808254983848 1107 1 0.03252539452418546 1107 2 0.04921482995892035 1107 3 0.07333350392438831 1107 4 0.06157897353693187 1107 5 -0.030035329970736768 1107 6 -0.062213543152978446 1107 7 -0.023464926785514706 1107 8 0.007862037689363888 1107 9 -0.037460498288020005 1107 10-11 0.00602344977799163 1107 12-13 0.06415979743938749 1107 14-15 0.07812822232227035 1107 16-17 0.03566853893466515 1107 18-19 0.09753487205485101 1107 20-21 0.0330656754482348 1107 22-23 0.039060937392413564 1107 24-25 0.07235601988782747 1107 26-27 0.11462300339981368 1107 28-29 0.088269810475893 1107 30-31 0.06203219736421772 1107 32-33 0.05122057962494608 1107 34-35 0.10351083718732212 1107 36-37 0.06633071511566868 1107 38-39 0.040950502026866786 1107 40-41 0.12599052308377878 1107 42-43 0.14656124868623266 1107 44-45 0.16554199194914077 1107 46-47 0.08630983500264477 1107 48-49 0.11998335091887213 1107 50-51 0.1518778936981704 1107 52-53 0.09341720636632544 1107 54-55 0.07395067239492903 1107 56-57 0.07396422692333715 1107 58-59 0.08409706777224812 1107 60-61 0.12848010621290484 1107 62-63 0.13837397838923238 1107 64-65 0.0934707450811274 1107 66-67 0.09215637096570362 1107 68-69 0.17255335812752293 1107 70-71 0.18360442512528863 1107 72-73 0.13153989934100707 1107 74-75 0.17517672325243439 1107 76-77 0.1143764675785377 1107 78-79 0.15176254298230418 1107 80-81 0.12164406796584615 1107 82-83 0.12693213875831333 1107 84-85 0.06480293085530775 1107 86-87 0.06545116185461808 1107 88-89 0.06700900255370357 1107 90-91 0.09856305866797044 1107 92-93 0.10941496931440042 1107 94-95 0.1284687865363381 1107 96-97 0.11887245756695108 1107 98-99 0.12055370770429619 1107 100 0.12545273638175303 1108 1 -0.004014288192330184 1108 2 -0.009427573006703938 1108 3 -0.021932136045755612 1108 4 0.008697986872192587 1108 5 -0.05584176810713615 1108 6 0.02704454350953256 1108 7 -0.029993998107457287 1108 8 -0.03943212632213289 1108 9 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2214 36-37 -0.16847437904831963 2214 38-39 -0.1422837807563866 2214 40-41 -0.08491662058753491 2214 42-43 -0.10434754143003033 2214 44-45 -0.12374893249803165 2214 46-47 -0.12217367046861938 2214 48-49 -0.1959063665575016 2214 50-51 -0.06538868315281832 2214 52-53 -0.18345421973995713 2214 54-55 -0.14533418474430704 2214 56-57 -0.21179845703522204 2214 58-59 -0.2278232529995563 2214 60-61 -0.28673765592012757 2214 62-63 -0.29806477702504 2214 64-65 -0.1828122550581881 2214 66-67 -0.239698123670685 2214 68-69 -0.1449183317265792 2214 70-71 -0.11613042204029256 2214 72-73 -0.2545754642067024 2214 74-75 -0.2145137411400313 2214 76-77 -0.11996508900851666 2214 78-79 -0.32870963051500013 2214 80-81 -0.18764063481917503 2214 82-83 -0.21852329720130825 2214 84-85 -0.24343293504809083 2214 86-87 -0.10389294355585577 2214 88-89 -0.22949972281294606 2214 90-91 -0.17439089707432487 2214 92-93 -0.12696922782204467 2214 94-95 -0.20741096915656243 2214 96-97 -0.3148877773047474 2214 98-99 -0.36344011654403374 2214 100 -0.5833748206738996 2215 1 -0.05071366377689657 2215 2 -0.05650661153633507 2215 3 -0.05799276726731506 2215 4 -0.05758946634892226 2215 5 -0.04728471154307812 2215 6 -0.05790150821554363 2215 7 -0.07091077711112348 2215 8 -0.08901774332763779 2215 9 -0.08727372816925794 2215 10-11 -0.1345939440585937 2215 12-13 -0.1529686344925949 2215 14-15 -0.1676011497625538 2215 16-17 -0.16703880119930403 2215 18-19 -0.16210935508443924 2215 20-21 -0.17368360320806175 2215 22-23 -0.17435892717441703 2215 24-25 -0.18501165537680464 2215 26-27 -0.16275402356512814 2215 28-29 -0.1354047340546316 2215 30-31 -0.18010888951617687 2215 32-33 -0.19860758317302896 2215 34-35 -0.2117808964342771 2215 36-37 -0.18310281974477505 2215 38-39 -0.18862136100506888 2215 40-41 -0.16347638420671018 2215 42-43 -0.1843421741493927 2215 44-45 -0.14241361646321593 2215 46-47 -0.18887785872168905 2215 48-49 -0.11373998326924806 2215 50-51 -0.0412061556493768 2215 52-53 -0.05353447229842345 2215 54-55 -0.08413404287998105 2215 56-57 -0.19028506647960342 2215 58-59 -0.16920720872327877 2215 60-61 -0.19042736853462827 2215 62-63 -0.14726904666577667 2215 64-65 -0.09236269018513354 2215 66-67 -0.16190712482079306 2215 68-69 -0.12590203575998515 2215 70-71 -0.06491281928898474 2215 72-73 -0.016485082714659427 2215 74-75 -0.0653048035498287 2215 76-77 -0.12915095900491735 2215 78-79 -0.09565264208651314 2215 80-81 -0.07906626620240331 2215 82-83 -0.054196903839930144 2215 84-85 -0.0561184580487577 2215 86-87 -0.11641379645740102 2215 88-89 -0.1261577638209701 2215 90-91 -0.1290088684730577 2215 92-93 -0.16323108221750715 2215 94-95 -0.13066924266635738 2215 96-97 -0.04884294121953303 2215 98-99 -0.08421030409053998 2215 100 -0.07928213138897888 2216 1 -0.181726781380533 2216 2 -0.16911088452675216 2216 3 -0.19654015681261683 2216 4 -0.11738083902987029 2216 5 -0.09017587305618946 2216 6 -0.11957432024362902 2216 7 -0.13765379785998277 2216 8 -0.19122640456645001 2216 9 -0.22417348245863167 2216 10-11 -0.24939038566763116 2216 12-13 -0.18343251150091078 2216 14-15 -0.4151633743591603 2216 16-17 -0.3273175801293178 2216 18-19 -0.2999222778917101 2216 20-21 -0.34753167242930516 2216 22-23 -0.33114777767859493 2216 24-25 -0.4493993292112748 2216 26-27 -0.45638823559342256 2216 28-29 -0.4864834517906189 2216 30-31 -0.42742301526367044 2216 32-33 -0.4201322350541403 2216 34-35 -0.3965926296269444 2216 36-37 -0.4292313458860093 2216 38-39 -0.419313469571847 2216 40-41 -0.4600825733177061 2216 42-43 -0.4341484334535366 2216 44-45 -0.3211295180142031 2216 46-47 -0.39288585963267053 2216 48-49 -0.39421621676321195 2216 50-51 -0.30044967221520125 2216 52-53 -0.3134693809625304 2216 54-55 -0.2836851944040717 2216 56-57 -0.24564054734245389 2216 58-59 -0.25981210888322437 2216 60-61 -0.23939925354940073 2216 62-63 -0.20971685608930812 2216 64-65 -0.21486712138703012 2216 66-67 -0.16514320880474287 2216 68-69 -0.21072633311032973 2216 70-71 -0.22593014917358545 2216 72-73 -0.2297855415660024 2216 74-75 -0.17261281681729912 2216 76-77 -0.17971972077083365 2216 78-79 -0.19492516697341244 2216 80-81 -0.19830017447574733 2216 82-83 -0.20517983285312624 2216 84-85 -0.27190973520058037 2216 86-87 -0.27573825319602463 2216 88-89 -0.20785862195639737 2216 90-91 -0.33590813127197094 2216 92-93 -0.28180343394054574 2216 94-95 -0.30473586939077535 2216 96-97 -0.3161256496504308 2216 98-99 -0.34668325913542475 2216 100 -0.4243196525325885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26.0 3 6.0 4 10.0 5 38.0 6 55.0 7 124.0 8 289.0 9 549.0 10 985.0 11 1673.0 12 2575.0 13 3900.0 14 4543.0 15 6220.0 16 7701.0 17 10973.0 18 12876.0 19 7120.0 20 5214.0 21 5009.0 22 5226.0 23 5726.0 24 6655.0 25 7925.0 26 10245.0 27 13180.0 28 17305.0 29 22496.0 30 28686.0 31 37297.0 32 48029.0 33 62569.0 34 87524.0 35 133735.0 36 249304.0 37 588776.0 38 1118725.0 39 576653.0 40 2298.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83425816201911 19.923117782379173 13.437880029170705 23.804744026431013 2 15.565123691549857 20.087781934462363 40.38599602614054 23.961098347847233 3 18.10444855509275 25.565932786588363 30.32254934933899 26.007069308979897 4 12.04632255686026 16.802437076155396 39.257073374231545 31.894166992752805 5 12.318465728678266 38.19978305338291 36.33703786019334 13.144713357745488 6 30.736518578621236 38.69593436725581 17.59470570981461 12.972841344308344 7 27.04312552613698 32.66277784802819 22.857153021251165 17.436943604583664 8 25.146803439588904 36.73510702632081 20.109209211475214 18.00888032261507 9 25.04639034899642 16.11916676530073 21.112863886932377 37.721578998770475 10-11 23.867496294341574 26.708131880854292 29.23370363237799 20.19066819242615 12-13 24.709369259824594 23.970891651359533 29.428941479315966 21.89079760949991 14-15 21.720088356760552 25.891615987328265 26.694125756551934 25.69416989935925 16-17 21.10979419449978 29.51734018058107 27.19462266835692 22.178242956562233 18-19 21.001048269724343 28.204874882710374 29.71235886235813 21.081717985207156 20-21 22.082745841202495 27.493160298036372 29.345151087884513 21.078942772876623 22-23 21.595914667491098 27.265894905073452 29.47085488059923 21.66733554683622 24-25 21.529155106804556 27.439795203278404 29.40537869510002 21.62567099481702 26-27 21.292496107134255 27.4023792160585 29.472413609102922 21.832711067704334 28-29 21.36348538511768 27.45767963334477 29.559653435646293 21.61918154589125 30-31 21.60456284588765 27.858612725851067 29.139563675678332 21.397260752582948 32-33 21.336532250264682 27.557243932726998 29.556459191004386 21.549764626003935 34-35 21.380780512184334 27.37570023622232 29.64497103628087 21.59854821531248 36-37 21.697678653856666 27.645361917217194 29.054188033469202 21.602771395456937 38-39 21.58190407244737 27.909096613960145 29.112266768625027 21.396732544967456 40-41 21.490234123222287 27.592182510947254 29.294492494175994 21.623090871654462 42-43 21.44852004551815 27.564455473919896 29.15108907803749 21.83593540252446 44-45 21.58510554679876 27.71757817314003 29.03747517035859 21.659841109702622 46-47 21.72874438077458 27.86456577135872 28.723680386657367 21.68300946120933 48-49 21.72733382537199 27.859069089601274 28.59250208124589 21.82109500378085 50-51 21.72441144152659 27.867622148572963 28.474950941669302 21.933015468231144 52-53 21.786326971404712 27.801189851896734 28.4655515867953 21.946931589903254 54-55 21.768027343216257 28.22590073450047 28.283046335962776 21.723025586320503 56-57 21.6145488037804 27.941861314978084 28.38847497443533 22.055114906806185 58-59 21.57210171578238 27.521821406055132 28.749084997450847 22.15699188071164 60-61 21.681908173237687 27.901755829318407 28.518983485619042 21.89735251182486 62-63 21.62291170222898 27.871760621033264 28.436277519430735 22.069050157307018 64-65 21.576860841031472 27.687169111006654 28.52723361642175 22.208736431540128 66-67 21.44775321540939 27.751009873492144 28.473840306912283 22.327396604186184 68-69 21.828293771424875 27.82594916240864 28.406555203415046 21.93920186275144 70-71 21.79796101512665 27.678904348810324 28.398968380373358 22.124166255689673 72-73 21.655939210037054 27.870905734098567 28.364450665072127 22.10870439079225 74-75 21.70261660161625 27.887628843641956 28.239382941106655 22.170371613635137 76-77 21.72644081620245 27.864630073574904 28.180001895076778 22.22892721514587 78-79 21.820442022897332 27.963662564136392 28.22572195283804 21.990173460128233 80-81 21.74907263273346 27.766510258078213 28.287090688684497 22.197326420503835 82-83 21.627884940953443 27.847944891390362 28.403532606498693 22.120637561157498 84-85 21.63512713557414 27.788112820179478 28.203767474643303 22.372992569603074 86-87 21.79693652002516 27.867125234660367 28.13964656631854 22.196291678995937 88-89 21.562508084754096 27.74993208806561 28.219801826507645 22.467758000672653 90-91 21.524277638998417 27.731706335070854 28.238830866377224 22.50518515955351 92-93 21.66109707867065 27.81401634640525 28.153705429349827 22.37118114557427 94-95 21.49220306315163 27.604697565518848 28.2349849946964 22.66811437663312 96-97 21.525302046412957 27.609305875352497 28.213511887822417 22.65188019041213 98-99 21.349038237652966 27.77863296509973 28.188869557343544 22.68345923990376 100 21.508809148060955 27.620721548133393 27.973443199751635 22.89702610405402 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28.0 1 27.5 2 83.0 3 149.0 4 113.0 5 79.0 6 200.5 7 502.0 8 892.0 9 1112.0 10 955.5 11 783.0 12 822.0 13 938.0 14 1086.0 15 1294.0 16 1522.0 17 1727.0 18 1992.0 19 2389.5 20 2955.0 21 3644.5 22 4887.5 23 6958.0 24 9717.0 25 13288.5 26 18287.5 27 24722.0 28 33183.5 29 42662.0 30 51374.5 31 60603.0 32 69741.0 33 78657.5 34 86398.0 35 92599.0 36 98001.5 37 103297.5 38 107097.5 39 108137.0 40 109510.0 41 111337.0 42 113210.0 43 113917.0 44 111911.0 45 110026.0 46 108475.5 47 104508.0 48 101614.5 49 99898.5 50 97653.5 51 95183.0 52 88254.0 53 82210.0 54 79837.5 55 75538.0 56 68770.5 57 62320.0 58 55593.5 59 48614.0 60 42765.0 61 36561.0 62 31134.0 63 25896.5 64 20734.5 65 16444.5 66 12554.0 67 9356.5 68 7118.0 69 5477.0 70 3986.5 71 2748.5 72 2035.5 73 1489.5 74 1084.5 75 719.5 76 374.5 77 201.5 78 133.0 79 82.5 80 34.5 81 13.5 82 7.5 83 5.0 84 2.5 85 1.0 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09181046749282074 2 0.0010348485240472926 3 0.0 4 3.233901637647789E-4 5 0.03741624194758492 6 9.701704912943369E-5 7 0.004107055079812692 8 3.233901637647789E-4 9 6.467803275295579E-5 10-11 3.0722065557653997E-4 12-13 0.0 14-15 1.1318655731767263E-4 16-17 0.0 18-19 4.850852456471684E-5 20-21 0.0 22-23 2.2637311463534525E-4 24-25 9.701704912943369E-5 26-27 0.0014552557369415051 28-29 0.009345975732802112 30-31 0.035540578997749206 32-33 0.025127415724523323 34-35 0.02821579178847696 36-37 0.025903552117558796 38-39 0.025289110806405717 40-41 0.030414844902077458 42-43 0.02347812588932295 44-45 0.021764158021369624 46-47 0.016509067860191965 48-49 0.01105994360075544 50-51 0.008327296716943057 52-53 0.005643158357695392 54-55 0.004478953768142188 56-57 0.0026517993428711874 58-59 0.0011480350813649653 60-61 8.408144257884252E-4 62-63 0.0012450521304943989 64-65 0.003120715080330117 66-67 6.791193439060358E-4 68-69 0.0012935606550591157 70-71 4.8508524564716836E-4 72-73 6.144413111530799E-4 74-75 7.437973766589916E-4 76-77 5.497632784001241E-4 78-79 6.467803275295579E-5 80-81 5.98271802964841E-4 82-83 6.144413111530799E-4 84-85 3.0722065557653997E-4 86-87 1.6169508188238946E-4 88-89 0.0 90-91 4.850852456471684E-5 92-93 1.6169508188238948E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3092240.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.70769775265461 #Duplication Level Percentage of deduplicated Percentage of total 1 73.49054756652797 43.144608542128104 2 15.344182787507425 18.016432907009424 3 5.104518380373581 8.990235667435268 4 2.2488292454822814 5.280943505644163 5 1.1516203499281512 3.3804489714694115 6 0.6835707458088791 2.4078518842502614 7 0.4190282977505462 1.7220130657903898 8 0.2782372783120979 1.306773603093431 9 0.21493845154323601 1.135668748376151 >10 0.9899604483148763 10.096837510114165 >50 0.05375788767882653 2.1557996475566563 >100 0.020422585495310574 1.9672651749561942 >500 1.6541797638338805E-4 0.052311582100380956 >1k 2.205573004901568E-4 0.342809190076057 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4199 0.13579152976483067 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3521 0.11386567666157865 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.2339016376477895E-5 0.0 9 0.0 0.0 0.0 6.467803275295579E-5 0.0 10-11 0.0 0.0 0.0 6.467803275295579E-5 0.0 12-13 0.0 0.0 0.0 1.2935606550591158E-4 0.0 14-15 0.0 0.0 0.0 1.2935606550591158E-4 0.0 16-17 0.0 0.0 0.0 1.2935606550591158E-4 0.0 18-19 1.6169508188238948E-5 0.0 0.0 1.4552557369415053E-4 0.0 20-21 3.2339016376477895E-5 0.0 0.0 1.6169508188238946E-4 0.0 22-23 3.2339016376477895E-5 0.0 0.0 2.7488163920006206E-4 0.0 24-25 3.2339016376477895E-5 0.0 0.0 3.233901637647789E-4 0.0 26-27 3.2339016376477895E-5 0.0 0.0 4.527462292706905E-4 0.0 28-29 3.2339016376477895E-5 0.0 0.0 0.0010025095076708146 0.0 30-31 3.2339016376477895E-5 0.0 0.0 0.003767495407859675 0.0 32-33 3.2339016376477895E-5 0.0 0.0 0.0107527229451789 0.0 34-35 3.2339016376477895E-5 0.0 0.0 0.02058378392362818 0.0 36-37 3.2339016376477895E-5 0.0 0.0 0.040084210798644354 0.0 38-39 3.2339016376477895E-5 0.0 0.0 0.07502651799342872 0.0 40-41 3.2339016376477895E-5 0.0 0.0 0.11601622125061443 0.0 42-43 3.2339016376477895E-5 0.0 0.0 0.1566016868030942 0.0 44-45 3.2339016376477895E-5 0.0 0.0 0.2026847851395752 0.0 46-47 3.2339016376477895E-5 0.0 0.0 0.2597793185522469 0.0 48-49 6.467803275295579E-5 0.0 0.0 0.3250071145836028 0.0 50-51 6.467803275295579E-5 0.0 0.0 0.3977213929061134 0.0 52-53 6.467803275295579E-5 0.0 0.0 0.46912594106537653 0.0 54-55 6.467803275295579E-5 0.0 0.0 0.5484858872532533 0.0 56-57 6.467803275295579E-5 0.0 0.0 0.6389219465500737 0.0 58-59 6.467803275295579E-5 0.0 0.0 0.7447998861666624 0.0 60-61 6.467803275295579E-5 0.0 0.0 0.8613658706956768 0.0 62-63 6.467803275295579E-5 0.0 0.0 0.9774952785036091 0.0 64-65 6.467803275295579E-5 0.0 0.0 1.0987180813908364 0.0 66-67 6.467803275295579E-5 0.0 0.0 1.2381154114816444 0.0 68-69 6.467803275295579E-5 0.0 0.0 1.4012010710682223 0.0 70-71 6.467803275295579E-5 0.0 0.0 1.58008434015471 0.0 72-73 6.467803275295579E-5 0.0 0.0 1.76022883087988 0.0 74-75 6.467803275295579E-5 0.0 0.0 1.9449169534059454 0.0 76-77 6.467803275295579E-5 0.0 0.0 2.1419100716632604 0.0 78-79 6.467803275295579E-5 0.0 0.0 2.369043153183453 0.0 80-81 6.467803275295579E-5 0.0 0.0 2.6200747678058622 0.0 82-83 6.467803275295579E-5 0.0 0.0 2.8799834424236153 0.0 84-85 6.467803275295579E-5 0.0 0.0 3.1384045222880497 0.0 86-87 6.467803275295579E-5 0.0 0.0 3.4150486378806306 0.0 88 6.467803275295579E-5 0.0 0.0 3.644380772514423 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4055 0.0 51.76488 1 GTATCAA 7000 0.0 40.81144 1 TCAACGC 7935 0.0 35.312347 4 ATCAACG 7970 0.0 35.157276 3 CAACGCA 8200 0.0 34.228493 5 AACGCAG 8350 0.0 33.711494 6 TATCAAC 8670 0.0 32.589348 2 ACGCAGA 9720 0.0 28.863283 7 CGCAGAG 9910 0.0 28.214602 8 GTACATG 8320 0.0 27.887835 1 TACATGG 8550 0.0 26.173458 2 ACATGGG 8525 0.0 25.03737 3 GCAGAGT 11150 0.0 24.655375 9 TAGTGCG 110 0.0030634499 21.360329 9 GAGTACT 7215 0.0 21.265247 12-13 GTGGTAT 1800 0.0 20.91745 1 CAGAGTA 11490 0.0 19.876497 10-11 AGAGTAC 10300 0.0 19.68644 10-11 TACACCG 525 0.0 18.805603 5 GAGTACA 5900 0.0 18.107775 1 >>END_MODULE