FastQCFastQC Report
Wed 25 May 2016
SRR1033144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167373
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.037.446061
GTATCAA26800.033.7401471
TCAACGC26150.032.1556934
ATCAACG26850.031.673133
CAACGCA28450.029.556115
GTACATG58650.029.5501921
TACATGG58000.028.6901342
AACGCAG29300.028.6894136
TATCAAC33450.025.7165242
ACATGGG63000.025.3568383
ACGCAGA35200.023.2136177
CGCAGAG35700.022.2307828
CATGGGG40850.021.1593484
GAGTACA41250.020.8933321
TACGACC2258.049028E-820.8782464
TACACCG2702.6138878E-819.1383935
GCAGAGT44050.017.5903439
AGTACAT44550.017.0932482
GTATAAG11000.016.6975381
AGAGTAC36200.016.4805495
Produced by FastQC (version 0.11.5)