FastQCFastQC Report
Wed 25 May 2016
SRR1033144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167373
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38690.17851103617143887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32750.15110458605879099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31050.051.5157661
GTATCAA53550.042.4336661
TCAACGC63400.035.3647844
ATCAACG63650.035.225883
CAACGCA66000.034.042065
AACGCAG68500.033.066496
TATCAAC70250.032.1171382
ACGCAGA77700.028.9093637
GTACATG58300.028.6472321
CGCGCGT1154.20363E-628.6043367
CGCAGAG78650.028.3211778
TAGCGCG1406.367063E-726.8598654
TAGACCG700.008834342526.8592455
TACATGG59400.026.5094222
ACATGGG61700.024.6832163
GCAGAGT90500.024.3531959
GTGGTAT16000.022.6408961
GAGTACT61450.021.48899312-13
CAGAGTA87200.020.93660410-11
AGAGTAC82300.020.21320210-11
Produced by FastQC (version 0.11.5)