FastQCFastQC Report
Wed 25 May 2016
SRR1033143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591641
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20550.037.5918461
GGTATCA12850.034.0911331
TCAACGC22250.033.357964
ATCAACG23000.032.8963663
CAACGCA23000.032.2702035
AACGCAG24300.030.5351736
GTACATG51700.028.6999821
TATCAAC26800.028.0698452
TACATGG51800.027.3206832
ACATGGG52400.026.367323
CATGGGG37600.025.4867654
TACACCG2601.8189894E-1225.294565
ACGCAGA29700.023.8764697
CGCAGAG30150.023.3643428
GTACTAG3150.022.4306771
CGTACAC1605.419139E-520.560233
GCAGAGT34900.019.3770059
ACCGTCC2451.9807521E-719.1682938
ACACCGT3204.620233E-1019.0784456
GAGTACA34650.017.6726531
Produced by FastQC (version 0.11.5)