##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033143_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1591641 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.88928533507242 31.0 31.0 34.0 28.0 34.0 2 30.972055256179 33.0 31.0 34.0 27.0 34.0 3 31.186231065925043 34.0 31.0 34.0 28.0 34.0 4 34.568824879479735 37.0 35.0 37.0 32.0 37.0 5 34.64984126445599 37.0 35.0 37.0 32.0 37.0 6 34.68603975393949 37.0 35.0 37.0 32.0 37.0 7 34.662409425240995 37.0 35.0 37.0 32.0 37.0 8 34.65204339420762 37.0 35.0 37.0 32.0 37.0 9 36.23581322672638 39.0 37.0 39.0 32.0 39.0 10-11 36.21636380314405 39.0 37.0 39.0 32.0 39.0 12-13 36.1399024654429 39.0 37.0 39.0 32.0 39.0 14-15 37.50014670393638 40.0 38.0 41.0 32.0 41.0 16-17 37.48006962625365 40.0 38.0 41.0 32.0 41.0 18-19 37.4377570067622 40.0 38.0 41.0 32.0 41.0 20-21 37.46228232371496 40.0 38.0 41.0 32.0 41.0 22-23 37.40482338668079 40.0 38.0 41.0 31.0 41.0 24-25 37.37775886647806 40.0 38.0 41.0 31.0 41.0 26-27 37.33208022412089 40.0 38.0 41.0 31.0 41.0 28-29 37.30109302286131 40.0 38.0 41.0 31.0 41.0 30-31 37.29374588867716 40.0 38.0 41.0 31.0 41.0 32-33 37.36665648849207 40.0 38.0 41.0 31.0 41.0 34-35 37.33226022702355 40.0 38.0 41.0 30.5 41.0 36-37 37.23848719654747 40.0 38.0 41.0 30.0 41.0 38-39 37.14814804343442 40.0 38.0 41.0 30.0 41.0 40-41 37.0726190767893 40.0 38.0 41.0 30.0 41.0 42-43 36.935142409626295 40.0 37.5 41.0 30.0 41.0 44-45 36.77817265325535 40.0 37.0 41.0 30.0 41.0 46-47 36.629883246284805 40.0 37.0 41.0 30.0 41.0 48-49 36.499317999473504 40.0 36.5 41.0 30.0 41.0 50-51 36.3513006387747 40.0 36.0 41.0 29.5 41.0 52-53 36.18646447283024 40.0 36.0 41.0 29.0 41.0 54-55 36.01896187645329 40.0 35.0 41.0 29.0 41.0 56-57 35.84353538266481 39.0 35.0 41.0 28.5 41.0 58-59 35.62283109067937 39.0 35.0 41.0 28.0 41.0 60-61 35.39305000311 39.0 35.0 41.0 28.0 41.0 62-63 35.122866588634 38.5 35.0 40.5 28.0 41.0 64-65 34.8443307881614 38.0 34.0 40.0 27.0 41.0 66-67 34.543917566838246 37.0 34.0 40.0 26.5 41.0 68-69 34.23545918960369 37.0 34.0 40.0 26.0 41.0 70-71 33.893140161631926 36.0 34.0 39.0 26.0 41.0 72-73 33.5309133780796 36.0 34.0 39.0 26.0 41.0 74-75 33.16520905153863 35.0 33.0 39.0 25.0 40.0 76-77 32.84570672657968 35.0 33.0 37.5 25.0 40.0 78-79 32.48561233343449 35.0 33.0 37.0 24.5 39.0 80-81 32.13648303857465 35.0 33.0 36.5 24.0 39.0 82-83 31.927194637484206 35.0 33.0 36.0 24.0 38.5 84-85 31.663283366035433 35.0 33.0 36.0 23.0 37.0 86-87 31.40934890468391 35.0 33.0 35.5 22.5 37.0 88-89 31.05619766015075 35.0 32.0 35.0 19.5 36.5 90-91 30.86448545871839 35.0 32.0 35.0 18.5 36.0 92-93 30.678862570139877 35.0 32.0 35.0 16.5 36.0 94-95 30.49545374867825 35.0 32.0 35.0 9.0 36.0 96-97 30.297734853525387 35.0 32.0 35.0 2.0 35.5 98-99 30.083857792052353 35.0 32.0 35.0 2.0 35.0 100 29.90073703806323 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3089389535021887 1101 2 0.3461193016002717 1101 3 0.33244963493712376 1101 4 0.34754461400496695 1101 5 0.32074395889666363 1101 6 0.2960639929335187 1101 7 0.39367123783650015 1101 8 0.26456970360521836 1101 9 0.49276567628512424 1101 10-11 0.4509643411934192 1101 12-13 0.4988921779029525 1101 14-15 0.5067529306573846 1101 16-17 0.4129535590029789 1101 18-19 0.44012920932956945 1101 20-21 0.44291765235888647 1101 22-23 0.4270037585728872 1101 24-25 0.4392173819840366 1101 26-27 0.4576945214330266 1101 28-29 0.49943469555509523 1101 30-31 0.5703932929867719 1101 32-33 0.5887282725495666 1101 34-35 0.6189916797271522 1101 36-37 0.5945884697682757 1101 38-39 0.6587263649338553 1101 40-41 0.6601351061438976 1101 42-43 0.6126541682434592 1101 44-45 0.5271148389910962 1101 46-47 0.5938432440088803 1101 48-49 0.5925296268585711 1101 50-51 0.6912727335090025 1101 52-53 0.6758909738949299 1101 54-55 0.49951544574682316 1101 56-57 0.5700676881481996 1101 58-59 0.6385230663270534 1101 60-61 0.5507687698710413 1101 62-63 0.5384775803430699 1101 64-65 0.48260461032137414 1101 66-67 0.4817301250271768 1101 68-69 0.42055840175626713 1101 70-71 0.44885897496189386 1101 72-73 0.4718643647922036 1101 74-75 0.393248276310473 1101 76-77 0.3255237498282142 1101 78-79 0.3684643729710615 1101 80-81 0.4566544294304222 1101 82-83 0.35456062958602885 1101 84-85 0.3491405552619966 1101 86-87 0.3196292512019845 1101 88-89 0.42806114047808563 1101 90-91 0.5703698493774034 1101 92-93 0.5952150854248721 1101 94-95 0.5772619567769262 1101 96-97 0.5259333369480643 1101 98-99 0.6137810398510375 1101 100 0.6558476081992701 1102 1 0.41820708541403206 1102 2 0.2995686763913241 1102 3 0.4015070246009813 1102 4 0.38412485412535347 1102 5 0.36209421702087496 1102 6 0.3928442355163 1102 7 0.4378557007572468 1102 8 0.4103576525050485 1102 9 0.6154384622934757 1102 10-11 0.5835293789294624 1102 12-13 0.5733727804674302 1102 14-15 0.6171231691393473 1102 16-17 0.6232967112531753 1102 18-19 0.577841672879174 1102 20-21 0.5232090092938648 1102 22-23 0.5368907067027564 1102 24-25 0.5455583067231444 1102 26-27 0.5021877295130608 1102 28-29 0.5086279141476098 1102 30-31 0.47584491801861617 1102 32-33 0.4893964311688137 1102 34-35 0.5282041238617907 1102 36-37 0.4795223689449841 1102 38-39 0.4977860370389209 1102 40-41 0.46802572322697245 1102 42-43 0.5259645887714157 1102 44-45 0.49462926051536726 1102 46-47 0.5535072989693077 1102 48-49 0.5501958354585383 1102 50-51 0.6463071263938431 1102 52-53 0.5147356608737397 1102 54-55 0.6465132779012137 1102 56-57 0.4678089237160279 1102 58-59 0.4914461537407604 1102 60-61 0.4186590838913844 1102 62-63 0.6460576528318853 1102 64-65 0.490394648459926 1102 66-67 0.5299387875071631 1102 68-69 0.5619286430794403 1102 70-71 0.49572005005799014 1102 72-73 0.5032430066355076 1102 74-75 0.40128947768902634 1102 76-77 0.2864345291066783 1102 78-79 0.3588231897762668 1102 80-81 0.407629581127523 1102 82-83 0.34251426477615965 1102 84-85 0.32339556550456905 1102 86-87 0.31660610573282 1102 88-89 0.38320463594682863 1102 90-91 0.42685894338083585 1102 92-93 0.4551912950003256 1102 94-95 0.5432014938675422 1102 96-97 0.5001535896834426 1102 98-99 0.48509473156236993 1102 100 0.42622947671360834 1103 1 0.10643490365536223 1103 2 0.07116746730009638 1103 3 0.08163126183301728 1103 4 0.07940698455433193 1103 5 -0.10205948312376734 1103 6 0.012577547881321038 1103 7 0.07420432571770164 1103 8 0.026748043411629396 1103 9 0.09835063128829091 1103 10-11 0.06446278565188379 1103 12-13 0.09748767704916617 1103 14-15 0.15796565629778314 1103 16-17 0.10331552787251042 1103 18-19 0.021840652502731928 1103 20-21 0.01810694422017889 1103 22-23 0.0427945353087722 1103 24-25 0.022893789308170653 1103 26-27 0.07011993116724113 1103 28-29 0.07216509114269343 1103 30-31 0.17227314331334753 1103 32-33 0.0750005287665374 1103 34-35 0.04460905238972401 1103 36-37 0.1652890318934368 1103 38-39 -0.00286715017455208 1103 40-41 0.085738031929246 1103 42-43 0.039962580933902814 1103 44-45 -0.03606550978106782 1103 46-47 -0.005522849680154707 1103 48-49 -0.033836736127817346 1103 50-51 -0.03344032129357544 1103 52-53 0.014582147249527111 1103 54-55 -0.027333084127619145 1103 56-57 -0.11679738104006532 1103 58-59 -0.06739582871534111 1103 60-61 0.03838581318258605 1103 62-63 -0.046630106490930245 1103 64-65 -0.03599598016876371 1103 66-67 -0.05969643705794425 1103 68-69 -0.09324903591159739 1103 70-71 -0.09209084017473401 1103 72-73 0.018765254682072907 1103 74-75 -0.08498773920679525 1103 76-77 -0.09780431212603702 1103 78-79 -0.08005205548894878 1103 80-81 0.047372603526433466 1103 82-83 0.0016433423960826588 1103 84-85 0.03226378882437686 1103 86-87 0.05992812593187935 1103 88-89 0.09923076343536508 1103 90-91 0.11320436823596225 1103 92-93 0.14137625743109083 1103 94-95 0.018101239139156178 1103 96-97 0.03215144286992455 1103 98-99 0.11811387550676145 1103 100 0.22034073431843026 1104 1 0.11479841962859894 1104 2 0.027570899666592652 1104 3 0.07459452687235668 1104 4 0.2058625361471087 1104 5 0.07487591205449462 1104 6 0.05683661794815009 1104 7 0.0919242232411861 1104 8 0.023469988476151116 1104 9 0.12985732877938005 1104 10-11 0.10339963442797284 1104 12-13 0.1476192184170273 1104 14-15 0.028792068582454533 1104 16-17 0.17702149899095332 1104 18-19 0.07637668055731694 1104 20-21 0.05648785931898459 1104 22-23 0.13619228116644422 1104 24-25 0.20846889115237843 1104 26-27 0.2379678532896321 1104 28-29 0.2920532010534487 1104 30-31 0.3145012260277511 1104 32-33 0.23906668096661576 1104 34-35 0.28010593276700746 1104 36-37 0.33699332206737154 1104 38-39 0.19364687924694834 1104 40-41 0.14143157428890873 1104 42-43 0.13174350114799438 1104 44-45 0.09941215269720516 1104 46-47 0.06544252826238761 1104 48-49 -0.1305443299331941 1104 50-51 0.020812233869961005 1104 52-53 0.0680214051899668 1104 54-55 -0.046006594402975054 1104 56-57 -0.026231344644706667 1104 58-59 -0.07946225373143534 1104 60-61 -0.05209798765603324 1104 62-63 -0.03135240962646435 1104 64-65 -0.08769976591725737 1104 66-67 -0.04002633129911004 1104 68-69 -0.09842330424604029 1104 70-71 -0.09814518881939449 1104 72-73 -0.04956396746547398 1104 74-75 -0.20501701719636856 1104 76-77 -0.21117233240536137 1104 78-79 -0.11710157159575374 1104 80-81 -0.0874564104568023 1104 82-83 0.006641949897673527 1104 84-85 -0.05881342237073994 1104 86-87 0.02910027141670568 1104 88-89 0.1181339293799688 1104 90-91 0.10552235945753807 1104 92-93 0.09499504820252014 1104 94-95 0.06720012926108865 1104 96-97 0.13146979358344169 1104 98-99 0.17451876431292135 1104 100 0.26874142147377 1105 1 0.23026455611165275 1105 2 0.18421292720840654 1105 3 0.3429158279932949 1105 4 0.2648240257696699 1105 5 0.25080117915318567 1105 6 0.16870745821437083 1105 7 0.32465448639908345 1105 8 0.3167175364225514 1105 9 0.3122247098985653 1105 10-11 0.34839461264410687 1105 12-13 0.34553428869463687 1105 14-15 0.35818585580113194 1105 16-17 0.47032412235990506 1105 18-19 0.3709586957293993 1105 20-21 0.4562707732124949 1105 22-23 0.38990388572901935 1105 24-25 0.3565434406969956 1105 26-27 0.3708983720522241 1105 28-29 0.45869808841223403 1105 30-31 0.4229129192583301 1105 32-33 0.4172938270608455 1105 34-35 0.4111207846585927 1105 36-37 0.47813349962232365 1105 38-39 0.4826669609090857 1105 40-41 0.4647759591956415 1105 42-43 0.3777021282169244 1105 44-45 0.40328446802691786 1105 46-47 0.4176922737523583 1105 48-49 0.39920221365644437 1105 50-51 0.490030888086622 1105 52-53 0.4300978375614477 1105 54-55 0.41443659512762565 1105 56-57 0.31679549111149896 1105 58-59 0.3046881343279395 1105 60-61 0.22166072862822972 1105 62-63 0.2763649227800471 1105 64-65 0.27638709041867315 1105 66-67 0.2970444909051153 1105 68-69 0.2598354777182337 1105 70-71 0.22484650813305507 1105 72-73 0.19710174592886887 1105 74-75 0.3149384161822013 1105 76-77 0.3033768153965468 1105 78-79 0.23484850886225672 1105 80-81 0.3031583270420981 1105 82-83 0.239218275539141 1105 84-85 0.23718960711958204 1105 86-87 0.31630867955226805 1105 88-89 0.403148214781492 1105 90-91 0.390958775737424 1105 92-93 0.4682541859334961 1105 94-95 0.4677712032386694 1105 96-97 0.4988953836088328 1105 98-99 0.4711139401164104 1105 100 0.55601277294938 1106 1 0.1647466191702449 1106 2 0.059628403185083556 1106 3 0.09541941326870784 1106 4 0.09581361460526949 1106 5 0.1597381548974468 1106 6 0.006642775356084485 1106 7 0.1416030670790036 1106 8 0.14411128549816965 1106 9 0.12400798495191623 1106 10-11 0.136519114138963 1106 12-13 0.178375114351212 1106 14-15 0.1338338629690341 1106 16-17 0.10935134734352658 1106 18-19 0.07846765231521147 1106 20-21 0.03510614304389037 1106 22-23 0.06470372435860838 1106 24-25 0.02883944644928249 1106 26-27 0.058973056046184524 1106 28-29 0.016481024746198614 1106 30-31 0.020287480837914984 1106 32-33 0.032731194346233394 1106 34-35 -0.043048187456811604 1106 36-37 -0.06703255211128578 1106 38-39 -0.16036291374493317 1106 40-41 -0.07616608677511039 1106 42-43 0.04194752348197994 1106 44-45 -0.011013666441378689 1106 46-47 0.0415337444944015 1106 48-49 -0.045059546443077636 1106 50-51 8.641311828156972E-4 1106 52-53 -0.1654321724482699 1106 54-55 -0.14557343770107423 1106 56-57 -0.10299354547284167 1106 58-59 -0.10088830598878218 1106 60-61 -0.16154966761403244 1106 62-63 -0.0384269628767413 1106 64-65 0.018933448095786787 1106 66-67 0.04198738730584495 1106 68-69 -0.025957769418823773 1106 70-71 0.026580612751786248 1106 72-73 0.06455426248848539 1106 74-75 0.05401909016116235 1106 76-77 0.10627572185453005 1106 78-79 0.14654764879232118 1106 80-81 0.1529351221888362 1106 82-83 0.11025681031697943 1106 84-85 0.054537569653120954 1106 86-87 0.02714614838744467 1106 88-89 0.08980999983927873 1106 90-91 0.09493106941571483 1106 92-93 0.05343344640276371 1106 94-95 0.09143638143279986 1106 96-97 0.13586957622858264 1106 98-99 0.09229839494325631 1106 100 0.10915292618523864 1107 1 0.22128298055953266 1107 2 0.2295592491093288 1107 3 0.27286058337868013 1107 4 0.35404136870023706 1107 5 0.199227677241403 1107 6 0.216438452656611 1107 7 0.18432200013678113 1107 8 0.15792632332629353 1107 9 0.22074528249420666 1107 10-11 0.27641607388081013 1107 12-13 0.17558364385855185 1107 14-15 0.23095356137037726 1107 16-17 0.05742865749811443 1107 18-19 0.2217248629897952 1107 20-21 0.17201393766266904 1107 22-23 0.16177728604451147 1107 24-25 0.1340982511115314 1107 26-27 0.19446288373710274 1107 28-29 0.23898852256007785 1107 30-31 0.16355672371290098 1107 32-33 0.1614433074572048 1107 34-35 0.20493769391142536 1107 36-37 0.056256704058085916 1107 38-39 0.0868816725218835 1107 40-41 0.11496780707282994 1107 42-43 0.18295017072543374 1107 44-45 0.24885508854779914 1107 46-47 0.12682188541425887 1107 48-49 0.09446011218029327 1107 50-51 0.08411078220709811 1107 52-53 0.11845293422156544 1107 54-55 0.10277340967914483 1107 56-57 0.15658986309494338 1107 58-59 0.21207464487410022 1107 60-61 0.25363328000095464 1107 62-63 0.30479123478251324 1107 64-65 0.284306018408067 1107 66-67 0.23053834224023007 1107 68-69 0.3018533948375932 1107 70-71 0.19788806989523522 1107 72-73 0.2013925731420514 1107 74-75 0.20577716994310435 1107 76-77 0.16959250706095474 1107 78-79 0.13307421389872331 1107 80-81 0.17010228323816534 1107 82-83 0.1710842846593046 1107 84-85 0.23008562044462622 1107 86-87 0.16769619365561184 1107 88-89 0.29403699949137874 1107 90-91 0.2562958651734988 1107 92-93 0.283854227590588 1107 94-95 0.17440987760297588 1107 96-97 0.2166380720712553 1107 98-99 0.22871031947538611 1107 100 0.3747791289072566 1108 1 -0.12970981899146494 1108 2 -0.07558202205738951 1108 3 -0.09325007903525062 1108 4 -0.17979629265963126 1108 5 -0.2897423573919369 1108 6 -0.23298729100193327 1108 7 -0.1721823598309129 1108 8 -0.18361759804370337 1108 9 -0.18770052933588488 1108 10-11 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-0.1012850415463511 2214 90-91 -0.1821660987101481 2214 92-93 -0.17968455703556785 2214 94-95 -0.20855243326193573 2214 96-97 -0.19656903483230792 2214 98-99 -0.27086012075140786 2214 100 -0.2780539958332646 2215 1 -0.28730307471826677 2215 2 -0.20027895209849333 2215 3 -0.1597134102469937 2215 4 -0.3433771876528837 2215 5 -0.1578510105002664 2215 6 -0.12178708637765823 2215 7 -0.31727881281194215 2215 8 -0.15614336939987794 2215 9 -0.3158659597309139 2215 10-11 -0.31677902100715016 2215 12-13 -0.38118800357541005 2215 14-15 -0.3624956176583751 2215 16-17 -0.2691554136183285 2215 18-19 -0.18197491245496167 2215 20-21 -0.26799245022203877 2215 22-23 -0.2756313871662215 2215 24-25 -0.1686780682164155 2215 26-27 -0.15359080763917632 2215 28-29 -0.2010917629060529 2215 30-31 -0.22290969883814427 2215 32-33 -0.22988039332764743 2215 34-35 -0.29743410960386285 2215 36-37 -0.4791028743415211 2215 38-39 -0.32655277218788115 2215 40-41 -0.33031159885388917 2215 42-43 -0.37815634951202526 2215 44-45 -0.3510549688301694 2215 46-47 -0.17647819526291642 2215 48-49 -0.27334901661919986 2215 50-51 -0.27196648407956303 2215 52-53 -0.31287933898403963 2215 54-55 -0.20081412581015456 2215 56-57 -0.27528620892845623 2215 58-59 -0.26799417554422433 2215 60-61 -0.36103731079552404 2215 62-63 -0.107285784425585 2215 64-65 -0.29000956268229316 2215 66-67 -0.3735694141433257 2215 68-69 -0.21607825630253785 2215 70-71 -0.3130862105826182 2215 72-73 -0.2424099357599303 2215 74-75 -0.15752714421427783 2215 76-77 -0.2936527492943526 2215 78-79 -0.26680691720884653 2215 80-81 -0.27909053069284795 2215 82-83 -0.2927749769825745 2215 84-85 -0.32811900730666466 2215 86-87 -0.4402357813984956 2215 88-89 -0.2900153600711981 2215 90-91 -0.2650198582883476 2215 92-93 -0.30512019299889204 2215 94-95 -0.3257569757536416 2215 96-97 -0.31818787690249906 2215 98-99 -0.30811478129575676 2215 100 -0.3225062460758892 2216 1 -0.024416225299724204 2216 2 0.015894991738477415 2216 3 -0.014600527159203125 2216 4 -0.07598033419913719 2216 5 -0.007993914432802285 2216 6 0.06143498124200164 2216 7 -0.0469424167267789 2216 8 -0.07445384291686707 2216 9 -0.13518751371714188 2216 10-11 -0.028945119376423634 2216 12-13 -0.13797865710348134 2216 14-15 -0.19388987520766676 2216 16-17 -0.16581827812594696 2216 18-19 -0.2519833248268526 2216 20-21 -0.2776802805520191 2216 22-23 -0.1635248669320717 2216 24-25 -0.215469702047983 2216 26-27 -0.3897254785421751 2216 28-29 -0.29126000274452224 2216 30-31 -0.3497369579627545 2216 32-33 -0.17388310170103694 2216 34-35 -0.10063073492806751 2216 36-37 -0.13028854762384867 2216 38-39 -0.19838204131463044 2216 40-41 -0.21833958537889941 2216 42-43 -0.21330000197087884 2216 44-45 -0.1247836709767256 2216 46-47 -0.10143717830062826 2216 48-49 -0.12803482934778998 2216 50-51 -0.053641419998875506 2216 52-53 -0.08096453267756942 2216 54-55 -0.13865779247960575 2216 56-57 -0.20568329263953444 2216 58-59 -0.18364301107285286 2216 60-61 -0.061156695043337095 2216 62-63 -0.041996963457123115 2216 64-65 -0.027982264649509148 2216 66-67 -0.04829582207572258 2216 68-69 -0.07386471580346665 2216 70-71 -0.11757607660020852 2216 72-73 -0.14669674976596525 2216 74-75 -0.15031651802831902 2216 76-77 -0.21838573057967636 2216 78-79 -0.1627182916931389 2216 80-81 -0.07540367762666023 2216 82-83 -0.10606926029332442 2216 84-85 -0.1686268957101298 2216 86-87 -0.1663535743629545 2216 88-89 -0.20709361488779265 2216 90-91 -0.22617043062600573 2216 92-93 -0.2669141180049124 2216 94-95 -0.10441204494863143 2216 96-97 -0.16852805741513066 2216 98-99 -0.31881308415482223 2216 100 -0.2628838833012779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34154.0 3 5563.0 4 3580.0 5 2731.0 6 2293.0 7 2285.0 8 2513.0 9 2869.0 10 3249.0 11 3414.0 12 3686.0 13 4030.0 14 4350.0 15 4407.0 16 4490.0 17 4350.0 18 4441.0 19 4503.0 20 4441.0 21 4688.0 22 5402.0 23 7164.0 24 10262.0 25 13311.0 26 13650.0 27 12671.0 28 13813.0 29 16784.0 30 20697.0 31 25057.0 32 32225.0 33 42816.0 34 59757.0 35 90844.0 36 166231.0 37 325450.0 38 460269.0 39 168916.0 40 285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48273107964399 19.348036715800514 13.313165404542637 23.856066800012858 2 15.263963082156796 19.746844042745387 40.722290858858194 24.266902016239627 3 18.410008959024253 25.117897615642143 29.446080819829305 27.026012605504302 4 12.23936809116845 16.367177900035763 38.367644276610335 33.02580973218545 5 13.01960108917289 38.019098239271074 35.27246921930038 13.68883145225566 6 31.497133162632153 37.58161453997078 17.667069856579587 13.25418244081748 7 28.085744876570207 32.28960887508231 21.893268790935917 17.73137745741157 8 25.273486469418337 37.26246469292664 19.706986637509857 17.757062200145153 9 25.039368891223724 17.471995616322662 20.565894035157395 36.92274145729622 10-11 24.308131617594846 27.156921951578166 28.170334196983987 20.364612233842998 12-13 24.605814317366917 24.229254823794662 28.63350848507775 22.53142237376067 14-15 21.868381190633965 26.27949116015347 25.45082133326798 26.401306315944588 16-17 21.98526385245 29.691954817526273 25.99996857964275 22.322812750380972 18-19 21.91933876881322 28.71068276342906 27.743601360996067 21.62637710676165 20-21 22.903362745322923 27.911863953025446 27.39337040913574 21.79140289251589 22-23 22.63212928174782 27.66135868496277 27.39834746889015 22.30816456439926 24-25 22.46569875866663 27.687625786929317 27.39662494324368 22.450050511160374 26-27 22.304504845315023 27.73659282701085 27.438037718111442 22.52086460956269 28-29 22.26462176915976 27.77691547328263 27.49083635646118 22.46762640109643 30-31 22.377459343730933 28.08375892100251 27.27239307522203 22.266388660044527 32-33 22.197007950665217 27.86079250250039 27.642065155674732 22.300134391159656 34-35 22.33058606332294 27.60418826717138 27.629386237760762 22.43583943174492 36-37 22.636428127546765 27.843157214138547 27.24632244597635 22.274092212338335 38-39 22.309673353216937 28.016370198221285 27.406666400658274 22.267290047903504 40-41 22.094249621344574 27.76448307918415 27.653988896544206 22.487278402927075 42-43 22.114351491724232 27.77493631849924 27.57402699567833 22.5366851940982 44-45 22.214242336560787 27.99651775383184 27.519075539671185 22.27016436993619 46-47 22.254040342793736 28.007503561081258 27.485955170903626 22.252500925221376 48-49 22.267279357133678 27.839267083637147 27.412390174542644 22.48106338468653 50-51 22.236684237858583 27.777060436669093 27.562140851654593 22.42411447381773 52-53 22.158439730572034 27.810960591133004 27.58055192520358 22.450047753091383 54-55 22.291786044594566 28.027015853388825 27.441435701791423 22.239762400225185 56-57 22.131949396674404 27.938231773786676 27.486954910011658 22.442863919527266 58-59 22.124001394827204 27.557073250418608 27.695487859662794 22.6234374950914 60-61 22.382946051560566 27.87326089189735 27.395403762728275 22.348389293813806 62-63 22.145136533192257 27.882288854360198 27.4886261608667 22.48394845158084 64-65 22.20574338843079 27.749239132346993 27.53363902076145 22.511378458460772 66-67 22.031049682067312 27.78302229094367 27.48624742045871 22.699680606530304 68-69 22.3340322156034 27.885559651392413 27.425422015356983 22.354986117647208 70-71 22.23771320432745 27.670304289940923 27.471542912289276 22.620439593442352 72-73 22.07504416127197 27.802736789394633 27.583587557123778 22.538631492209614 74-75 22.197760564561655 27.797794241227336 27.493698976474402 22.510746217736607 76-77 22.169982030435165 27.781135726762084 27.419576772766685 22.629305470036066 78-79 22.19673753406583 27.90270720271427 27.478036865707978 22.42251839751192 80-81 22.12249319155901 27.76936027200464 27.57380117561647 22.534345360819884 82-83 22.020158293242726 27.775236663916818 27.718941218381723 22.485663824458737 84-85 22.052549767654604 27.682276155506802 27.58155964201971 22.683614434818885 86-87 22.21425333460249 27.74646245790024 27.52143824494308 22.517845962554187 88-89 21.972810442974367 27.768573989398753 27.558818212502796 22.699797355124083 90-91 21.993243040360017 27.568920008508623 27.62796201715063 22.80987493398073 92-93 22.0751284463054 27.718674778000285 27.58773160790501 22.61846516778931 94-95 21.950230612802763 27.518056374125226 27.78534285384726 22.74637015922475 96-97 22.02632480404289 27.508541720124835 27.717644798430573 22.7474886774017 98-99 21.919730617344733 27.666547956451577 27.67501672423638 22.738704701967315 100 22.14501861903161 27.45428667494179 27.4975161984311 22.903178507595502 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 321.0 1 305.5 2 258.5 3 209.0 4 166.0 5 134.5 6 121.0 7 126.0 8 136.5 9 143.5 10 150.0 11 131.0 12 133.5 13 162.5 14 190.0 15 208.0 16 270.5 17 369.5 18 474.5 19 673.5 20 1000.5 21 1496.5 22 2187.0 23 3173.5 24 4575.0 25 6297.0 26 8594.5 27 11562.0 28 15366.5 29 19635.5 30 23567.5 31 27550.0 32 31616.0 33 35887.0 34 40134.5 35 43901.5 36 46679.0 37 49438.0 38 52181.0 39 53600.5 40 54879.5 41 56382.5 42 56651.5 43 56208.5 44 56025.0 45 55415.0 46 55053.5 47 53921.5 48 52526.5 49 51852.0 50 50931.0 51 49649.5 52 47170.5 53 45513.0 54 44252.0 55 41727.5 56 38328.5 57 34642.0 58 31715.5 59 29452.5 60 26831.5 61 23197.5 62 19836.0 63 16986.5 64 14146.5 65 11424.5 66 8879.5 67 6600.0 68 4937.0 69 3771.0 70 2754.5 71 1952.5 72 1463.5 73 1101.5 74 782.0 75 534.5 76 357.0 77 245.5 78 172.5 79 122.5 80 76.5 81 44.0 82 31.0 83 29.0 84 22.0 85 14.0 86 12.0 87 12.5 88 10.0 89 5.5 90 4.0 91 3.0 92 3.5 93 4.0 94 2.0 95 1.0 96 0.5 97 1.0 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33261269344029215 2 0.1224522364025556 3 0.06697490200365534 4 0.04096401135683235 5 0.044545220938641314 6 0.010429487554040138 7 0.00810484273777818 8 0.015455746616228156 9 0.017780391432490113 10-11 0.020984631584634976 12-13 0.01627251371383371 14-15 0.018565744410956993 16-17 0.019853723295642674 18-19 0.019256855032007848 20-21 0.02491139647696937 22-23 0.01658665490522046 24-25 0.025665335336297567 26-27 0.01947675386597857 28-29 0.018722815006650368 30-31 0.019602410342533273 32-33 0.024534427047305265 34-35 0.015267261901396106 36-37 0.010178174600930737 38-39 0.013351000633936922 40-41 0.009706962813850611 42-43 0.00797918626122348 44-45 0.008764539239690358 46-47 0.00816767097605553 48-49 0.005120501419604044 50-51 0.007256661521033952 52-53 0.007602216831559377 54-55 0.003581209581808963 56-57 0.002890098960758111 58-59 0.002890098960758111 60-61 0.0035497954626702876 62-63 0.0031414119138675114 64-65 0.0023246448162619585 66-67 0.001916261267459182 68-69 0.0032670683904222116 70-71 0.002136160101429908 72-73 0.0019790895057365324 74-75 0.0021675742205685827 76-77 0.003832522534918364 78-79 0.0033613107478382372 80-81 0.003172826033006187 82-83 0.002387473054539309 84-85 0.0028272707224807603 86-87 0.0021675742205685827 88-89 0.6008264426463004 90-91 0.6104705772218735 92-93 0.6067322970443711 94-95 0.5933184681721569 96-97 0.5910252374750337 98-99 0.5878524114420274 100 0.5898943291860412 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1591641.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.38894366770079 #Duplication Level Percentage of deduplicated Percentage of total 1 81.5596913381396 57.40900519155372 2 11.698347973455867 16.468687130174935 3 3.160922352099791 6.674819561397855 4 1.273717573158987 3.5862253802259394 5 0.6843402351858198 2.4084993132017884 6 0.3839226913401699 1.6214347616097173 7 0.28044053097264193 1.3817938915741372 8 0.18816519839093773 1.0595799639809167 9 0.139075897756727 0.8810464979459852 >10 0.6089817515270727 7.235437397604574 >50 0.01891143224077495 0.8936822929072888 >100 0.0033937417611379413 0.3433379124879293 >500 8.928397047413346E-5 0.03645070533516816 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 6.282823827735023E-5 0.0 0.0 7 0.0 0.0 6.282823827735023E-5 0.0 0.0 8 0.0 0.0 6.282823827735023E-5 0.0 0.0 9 0.0 0.0 6.282823827735023E-5 0.0 0.0 10-11 0.0 0.0 6.282823827735023E-5 0.0 0.0 12-13 0.0 0.0 6.282823827735023E-5 0.0 0.0 14-15 0.0 0.0 6.282823827735023E-5 0.0 0.0 16-17 0.0 0.0 6.282823827735023E-5 0.0 0.0 18-19 0.0 0.0 6.282823827735023E-5 0.0 0.0 20-21 0.0 0.0 6.282823827735023E-5 3.1414119138675116E-5 0.0 22-23 0.0 0.0 6.282823827735023E-5 9.424235741602535E-5 0.0 24-25 0.0 0.0 6.282823827735023E-5 2.8272707224807604E-4 0.0 26-27 0.0 0.0 6.282823827735023E-5 4.7121178708012674E-4 0.0 28-29 0.0 0.0 6.282823827735023E-5 9.738376932989286E-4 0.0 30-31 0.0 0.0 6.282823827735023E-5 0.003329896628699562 0.0 32-33 0.0 0.0 6.282823827735023E-5 0.009078680431077108 0.0 34-35 0.0 0.0 6.282823827735023E-5 0.016555240786081785 0.0 36-37 0.0 0.0 6.282823827735023E-5 0.030628766160208237 0.0 38-39 0.0 0.0 6.282823827735023E-5 0.05676531328358593 0.0 40-41 0.0 0.0 6.282823827735023E-5 0.08981296661747215 0.0 42-43 0.0 0.0 6.282823827735023E-5 0.12119567163700859 0.0 44-45 0.0 0.0 6.282823827735023E-5 0.15713342393165292 0.0 46-47 0.0 0.0 6.282823827735023E-5 0.20406611792483353 0.0 48-49 0.0 0.0 6.282823827735023E-5 0.25725022162661054 0.0 50-51 0.0 0.0 6.282823827735023E-5 0.3150836149609114 0.0 52-53 0.0 0.0 6.282823827735023E-5 0.37577569313683173 0.0 54-55 0.0 0.0 6.282823827735023E-5 0.43810130550796317 0.0 56-57 0.0 0.0 6.282823827735023E-5 0.5098511536206971 0.0 58-59 0.0 0.0 6.282823827735023E-5 0.5933498822912956 0.0 60-61 0.0 0.0 6.282823827735023E-5 0.6849848678188108 0.0 62-63 0.0 0.0 6.282823827735023E-5 0.7778764181118731 0.0 64-65 0.0 0.0 6.282823827735023E-5 0.8759198839436783 0.0 66-67 0.0 0.0 6.282823827735023E-5 0.9878798045539163 0.0 68-69 0.0 0.0 6.282823827735023E-5 1.1161750671162656 0.0 70-71 0.0 0.0 6.282823827735023E-5 1.259926076294843 0.0 72-73 0.0 0.0 6.282823827735023E-5 1.4166511166776932 0.0 74-75 0.0 0.0 6.282823827735023E-5 1.5693865639299314 0.0 76-77 0.0 0.0 6.282823827735023E-5 1.7249806960237892 0.0 78-79 0.0 0.0 6.282823827735023E-5 1.912303088447709 0.0 80-81 0.0 0.0 6.282823827735023E-5 2.1146414298199154 0.0 82-83 0.0 0.0 6.282823827735023E-5 2.335325616769108 0.0 84-85 0.0 0.0 6.282823827735023E-5 2.540742541816905 0.0 86-87 0.0 0.0 6.282823827735023E-5 2.759636123975193 0.0 88 0.0 0.0 6.282823827735023E-5 2.9435029632938585 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2055 0.0 37.591846 1 GGTATCA 1285 0.0 34.091133 1 TCAACGC 2225 0.0 33.35796 4 ATCAACG 2300 0.0 32.896366 3 CAACGCA 2300 0.0 32.270203 5 AACGCAG 2430 0.0 30.535173 6 GTACATG 5170 0.0 28.699982 1 TATCAAC 2680 0.0 28.069845 2 TACATGG 5180 0.0 27.320683 2 ACATGGG 5240 0.0 26.36732 3 CATGGGG 3760 0.0 25.486765 4 TACACCG 260 1.8189894E-12 25.29456 5 ACGCAGA 2970 0.0 23.876469 7 CGCAGAG 3015 0.0 23.364342 8 GTACTAG 315 0.0 22.430677 1 CGTACAC 160 5.419139E-5 20.56023 3 GCAGAGT 3490 0.0 19.377005 9 ACCGTCC 245 1.9807521E-7 19.168293 8 ACACCGT 320 4.620233E-10 19.078445 6 GAGTACA 3465 0.0 17.672653 1 >>END_MODULE