FastQCFastQC Report
Wed 25 May 2016
SRR1033143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591641
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29790.18716532182822634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25340.15920675579480548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24600.044.5569041
GTATCAA41050.043.2038421
TCAACGC47100.036.928444
ATCAACG47250.036.91073
CAACGCA48000.036.2348985
AACGCAG51000.034.3734666
TATCAAC51300.034.0883332
TATACCG851.7533053E-533.1817175
ACGCAGA59300.029.3245727
CGCAGAG61050.028.4043228
TCGCGCA700.00884239926.8537969
GTACATG46750.026.464781
GCAGAGT66500.025.7937779
TACATGG47100.025.3508762
GTACGAC1751.6439844E-724.1760123
ACATGGG47500.023.7518713
GTGGTAT13800.023.1805651
TATAACG1252.4208934E-422.5642782
GTATAGA7150.022.3700541
AATACCG1703.448482E-622.1211455
Produced by FastQC (version 0.11.5)