##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033143_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1591641 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74101069273787 34.0 31.0 34.0 31.0 34.0 2 32.818185130943476 34.0 31.0 34.0 31.0 34.0 3 32.92066050070336 34.0 31.0 34.0 31.0 34.0 4 36.36013271837054 37.0 37.0 37.0 35.0 37.0 5 36.29927163223365 37.0 37.0 37.0 35.0 37.0 6 36.38323466158512 37.0 37.0 37.0 35.0 37.0 7 36.352614063095885 37.0 37.0 37.0 35.0 37.0 8 36.34357245132539 37.0 37.0 37.0 35.0 37.0 9 38.15319158026213 39.0 39.0 39.0 37.0 39.0 10-11 38.1404019499372 39.0 39.0 39.0 37.0 39.0 12-13 38.112865903806195 39.0 39.0 39.0 37.0 39.0 14-15 39.645300039393305 41.0 40.0 41.0 37.0 41.0 16-17 39.653031368254524 41.0 40.0 41.0 37.0 41.0 18-19 39.65995598253626 41.0 40.0 41.0 37.0 41.0 20-21 39.64955790910136 41.0 40.0 41.0 37.0 41.0 22-23 39.58865284319768 41.0 40.0 41.0 37.0 41.0 24-25 39.54630692473994 41.0 40.0 41.0 37.0 41.0 26-27 39.452606146737864 41.0 39.0 41.0 36.5 41.0 28-29 39.35767047971245 41.0 39.0 41.0 36.0 41.0 30-31 39.26888224166128 41.0 39.0 41.0 36.0 41.0 32-33 39.31595692747297 41.0 39.0 41.0 36.0 41.0 34-35 39.266786919914736 41.0 39.0 41.0 35.0 41.0 36-37 39.19115428667646 41.0 39.0 41.0 35.0 41.0 38-39 39.10563688671 41.0 39.0 41.0 35.0 41.0 40-41 38.99123074864244 41.0 39.0 41.0 35.0 41.0 42-43 38.88219391181805 41.0 39.0 41.0 35.0 41.0 44-45 38.748153635147624 41.0 39.0 41.0 35.0 41.0 46-47 38.54592712803955 41.0 38.5 41.0 35.0 41.0 48-49 38.36565186496201 41.0 38.0 41.0 35.0 41.0 50-51 38.22707476120557 40.0 38.0 41.0 34.0 41.0 52-53 38.08618118030385 40.0 38.0 41.0 34.0 41.0 54-55 37.94779978650965 40.0 37.5 41.0 34.0 41.0 56-57 37.78188894354946 40.0 37.0 41.0 34.0 41.0 58-59 37.586337936758355 40.0 37.0 41.0 33.5 41.0 60-61 37.38142018206367 40.0 36.0 41.0 33.0 41.0 62-63 37.14068750428017 39.0 35.5 41.0 33.0 41.0 64-65 36.886179421113184 39.0 35.0 41.0 33.0 41.0 66-67 36.600499107524875 39.0 35.0 41.0 32.5 41.0 68-69 36.27396787340864 37.5 35.0 40.5 32.0 41.0 70-71 35.93896959176095 37.0 35.0 40.0 32.0 41.0 72-73 35.586597731523625 36.5 35.0 39.0 31.5 41.0 74-75 35.25119515016263 36.0 35.0 39.0 31.0 41.0 76-77 34.915747332470076 36.0 35.0 39.0 31.0 40.5 78-79 34.58646045182299 35.0 35.0 37.0 31.0 39.5 80-81 34.25719838833003 35.0 35.0 37.0 31.0 39.0 82-83 33.946728879188214 35.0 34.0 36.5 31.0 39.0 84-85 33.686091273094874 35.0 34.0 36.0 30.0 37.0 86-87 33.4613251355048 35.0 34.0 36.0 30.0 37.0 88-89 33.25715189543371 35.0 34.0 35.5 30.0 37.0 90-91 33.07165654817889 35.0 34.0 35.0 30.0 36.0 92-93 32.91395767010275 35.0 34.0 35.0 30.0 36.0 94-95 32.77173401539669 35.0 34.0 35.0 29.5 36.0 96-97 32.650860338480854 35.0 34.0 35.0 29.5 36.0 98-99 32.54896958547813 35.0 34.0 35.0 29.0 35.5 100 32.452757876933305 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11254532353539304 1101 2 0.1388717766137404 1101 3 0.12422851642038779 1101 4 0.06602527996670915 1101 5 0.11165146363092049 1101 6 0.11785213190592714 1101 7 0.14124162675323504 1101 8 0.20186738003448568 1101 9 0.20353816753546283 1101 10-11 0.1724776516662061 1101 12-13 0.21026847444917252 1101 14-15 0.2241380664658763 1101 16-17 0.22750106420349425 1101 18-19 0.29935750903412384 1101 20-21 0.28053220984615734 1101 22-23 0.3420504180321302 1101 24-25 0.2709895086486753 1101 26-27 0.294422535839459 1101 28-29 0.29171005405837747 1101 30-31 0.30215394498438286 1101 32-33 0.26424212658096735 1101 34-35 0.25144378922529853 1101 36-37 0.26988748345687696 1101 38-39 0.26629814525236384 1101 40-41 0.3156590419829115 1101 42-43 0.2708713808545795 1101 44-45 0.22322733161155384 1101 46-47 0.3265790531018027 1101 48-49 0.18293801587858383 1101 50-51 0.14319347329233523 1101 52-53 0.1711910322553436 1101 54-55 0.1615048745808778 1101 56-57 0.19089817825621225 1101 58-59 0.09534299393267531 1101 60-61 0.17258290414547872 1101 62-63 0.18389253428496488 1101 64-65 0.23570894880570847 1101 66-67 0.24425335043508056 1101 68-69 0.25486412228435285 1101 70-71 0.2174396057526664 1101 72-73 0.27184928261767993 1101 74-75 0.1985561925522461 1101 76-77 0.22244734927105725 1101 78-79 0.23288342800719875 1101 80-81 0.18259138025037203 1101 82-83 0.31693019522207067 1101 84-85 0.2085682960227473 1101 86-87 0.17169848641947993 1101 88-89 0.16400090497577224 1101 90-91 0.22838640615217543 1101 92-93 0.1313790863982689 1101 94-95 0.15991043363526813 1101 96-97 0.2408359993287732 1101 98-99 0.25182210930503146 1101 100 0.3238506055737673 1102 1 0.0845245220670563 1102 2 0.09602192620361194 1102 3 0.1199629084528695 1102 4 0.0823236149211084 1102 5 0.11906058979688083 1102 6 0.12418500654118247 1102 7 0.08999693863793823 1102 8 0.13100960832217368 1102 9 0.18487613267756586 1102 10-11 0.12947930051688417 1102 12-13 0.1595130432335239 1102 14-15 0.1749266451679503 1102 16-17 0.1897585785018947 1102 18-19 0.2275794683915109 1102 20-21 0.1758003588052901 1102 22-23 0.2167057552036269 1102 24-25 0.21008886303034302 1102 26-27 0.23861523827825692 1102 28-29 0.22404305938944447 1102 30-31 0.28266852656366126 1102 32-33 0.3131218836004237 1102 34-35 0.32875424005953136 1102 36-37 0.35320463765993537 1102 38-39 0.3584953216547646 1102 40-41 0.3468588768137266 1102 42-43 0.28557465876215815 1102 44-45 0.27172494546606174 1102 46-47 0.4618224077410815 1102 48-49 0.3967218772198038 1102 50-51 0.4328545828618289 1102 52-53 0.5307833939379876 1102 54-55 0.5530016919486513 1102 56-57 0.47150168980621743 1102 58-59 0.4768844846414382 1102 60-61 0.47130350587653425 1102 62-63 0.46413265467663933 1102 64-65 0.3568300536209037 1102 66-67 0.3760091159668022 1102 68-69 0.38752907601519127 1102 70-71 0.3659751024488074 1102 72-73 0.39361941521709554 1102 74-75 0.42845077407470455 1102 76-77 0.341680941502041 1102 78-79 0.34160320578645553 1102 80-81 0.2528270209960155 1102 82-83 0.20380852595253884 1102 84-85 0.3026295923546485 1102 86-87 0.28216665204704583 1102 88-89 0.3554201572253035 1102 90-91 0.3329909708451808 1102 92-93 0.31871248476381453 1102 94-95 0.356463379168261 1102 96-97 0.34534775105977644 1102 98-99 0.3917554544451818 1102 100 0.3146549245965389 1103 1 0.07777650567520311 1103 2 0.10950552429232374 1103 3 0.10700613913292045 1103 4 0.032308345847113173 1103 5 0.0755269054881822 1103 6 0.06459301082749391 1103 7 0.1159966058269788 1103 8 0.09585562839689032 1103 9 0.08821375110431973 1103 10-11 0.07443547736018985 1103 12-13 0.08096325782344849 1103 14-15 0.09792287635819008 1103 16-17 0.12699546127015537 1103 18-19 0.19072294099952813 1103 20-21 0.13188668484664845 1103 22-23 0.11883473956334001 1103 24-25 0.10809775749452655 1103 26-27 0.06571345591102329 1103 28-29 0.05935091866526676 1103 30-31 0.08929375034245624 1103 32-33 0.10712516050828214 1103 34-35 0.14035608478310735 1103 36-37 0.12097162244754855 1103 38-39 0.09360971170553967 1103 40-41 0.124672363703354 1103 42-43 0.006024428994599873 1103 44-45 0.05454842582746977 1103 46-47 0.031432561766720823 1103 48-49 0.1201365907555143 1103 50-51 0.06840208431101757 1103 52-53 0.05862266453340936 1103 54-55 0.04361505156551715 1103 56-57 0.049958032140686726 1103 58-59 0.14005154659729868 1103 60-61 0.041616768758792944 1103 62-63 0.06270202473132969 1103 64-65 -0.045015309975063644 1103 66-67 0.06741573597897599 1103 68-69 0.05723669610600268 1103 70-71 -0.03114233410224898 1103 72-73 -6.280754431671198E-4 1103 74-75 -0.05007277047167946 1103 76-77 -0.055732276213007026 1103 78-79 -0.04693218829279999 1103 80-81 0.06150936391198769 1103 82-83 -0.06560706166909114 1103 84-85 -0.034855069997675514 1103 86-87 -0.02491161521477636 1103 88-89 0.024835836278505496 1103 90-91 0.01988699668009275 1103 92-93 0.02703769592504557 1103 94-95 0.01489428523694869 1103 96-97 0.06525743494422898 1103 98-99 0.006364849588699428 1103 100 0.04421420609060789 1104 1 0.01663374759053937 1104 2 0.05388515914513903 1104 3 0.04117826330399055 1104 4 0.01954922391682601 1104 5 -0.004124358914580739 1104 6 0.02263120791148765 1104 7 0.07392219525877408 1104 8 0.11735303309245637 1104 9 0.07568623860380796 1104 10-11 0.08336736003224843 1104 12-13 0.08989762709980909 1104 14-15 0.11644346046865195 1104 16-17 0.1308749192188401 1104 18-19 0.09726445095091663 1104 20-21 0.08257110492078823 1104 22-23 0.07514048390347483 1104 24-25 0.06122633198821603 1104 26-27 -6.337649889545105E-4 1104 28-29 0.06057024284883994 1104 30-31 0.04648614673275375 1104 32-33 -0.028104551997628846 1104 34-35 0.02293862938923752 1104 36-37 0.03609085157179237 1104 38-39 0.0504085141569135 1104 40-41 -0.01972634695678721 1104 42-43 0.028547202737762234 1104 44-45 0.005811514246857996 1104 46-47 0.03472837816154595 1104 48-49 -0.03375881698364935 1104 50-51 -0.05917030784495836 1104 52-53 -0.0854353813366302 1104 54-55 -0.10416863801533083 1104 56-57 -0.02883180148418063 1104 58-59 -0.016907357648541677 1104 60-61 -0.05404218848725151 1104 62-63 -0.08907550659633756 1104 64-65 -0.05310618754745633 1104 66-67 0.016484928940897703 1104 68-69 -0.020040926870784403 1104 70-71 -0.05049646349944936 1104 72-73 0.013502586377221348 1104 74-75 -0.016578210451442033 1104 76-77 -0.052316037123873116 1104 78-79 -0.15343735829838323 1104 80-81 -0.03966470407319633 1104 82-83 -0.02251190003270409 1104 84-85 -0.06609197092140562 1104 86-87 -0.03294707703403077 1104 88-89 -0.008215752945822885 1104 90-91 0.06822534111315548 1104 92-93 0.0561107182575995 1104 94-95 0.044520044184103824 1104 96-97 -0.006448951081907239 1104 98-99 0.013882209639056953 1104 100 -0.003961620989507253 1105 1 0.1098432558662239 1105 2 0.09273967245628967 1105 3 0.09044921522622218 1105 4 0.074324981426237 1105 5 0.12031051935846904 1105 6 0.0574115082880553 1105 7 0.014717826443636284 1105 8 0.045420426252974266 1105 9 0.09604977744258036 1105 10-11 0.031204777384907345 1105 12-13 0.04840701934081437 1105 14-15 0.09147069030488097 1105 16-17 0.09024328280145966 1105 18-19 0.05075000682271025 1105 20-21 0.030032914733382654 1105 22-23 0.14097442842576413 1105 24-25 0.05494157631785157 1105 26-27 0.0550632230353898 1105 28-29 0.1371497101839907 1105 30-31 0.21160876309583188 1105 32-33 0.13751100650135584 1105 34-35 0.14882223073347944 1105 36-37 0.12976696156390943 1105 38-39 0.14361494368226602 1105 40-41 0.21621984196080035 1105 42-43 0.19831291667943418 1105 44-45 0.2580466869852742 1105 46-47 0.2915402843048156 1105 48-49 0.18478661716120115 1105 50-51 0.2671395977947668 1105 52-53 0.25114044623146725 1105 54-55 0.23607261286040426 1105 56-57 0.22486406724338082 1105 58-59 0.23859231668809144 1105 60-61 0.20537570876732758 1105 62-63 0.16211186478032147 1105 64-65 0.04639662848284587 1105 66-67 0.10434014831212579 1105 68-69 0.04748906699908417 1105 70-71 0.11149574794638539 1105 72-73 0.047356524988309445 1105 74-75 0.0359103776473404 1105 76-77 0.03364814537896876 1105 78-79 0.0706833506078226 1105 80-81 0.12231142945426399 1105 82-83 0.07306858807920946 1105 84-85 0.1474227851868335 1105 86-87 0.14144189365078574 1105 88-89 0.2133170952676764 1105 90-91 0.2163566764971563 1105 92-93 0.3430940355358203 1105 94-95 0.26138482273300667 1105 96-97 0.30089653670160743 1105 98-99 0.332032400658413 1105 100 0.41356823604700566 1106 1 0.04424228975665301 1106 2 0.023317878999847608 1106 3 0.02013027757700314 1106 4 0.04182475693385612 1106 5 0.02999030078212428 1106 6 0.03609331542532601 1106 7 -0.00646286483443248 1106 8 0.05986337481516557 1106 9 0.012610580544276218 1106 10-11 0.040910689061682604 1106 12-13 0.027935294816735734 1106 14-15 0.09495411880538285 1106 16-17 0.04603371718101812 1106 18-19 0.08374130840313398 1106 20-21 0.047627587793236614 1106 22-23 0.13186482464161742 1106 24-25 0.09486523570215155 1106 26-27 0.05635842853835982 1106 28-29 0.007142044429372163 1106 30-31 0.04720019818320509 1106 32-33 0.07303960022825606 1106 34-35 0.04564115715808015 1106 36-37 0.06228323065897001 1106 38-39 0.04379168191358218 1106 40-41 0.08125774485860404 1106 42-43 0.020263220668532256 1106 44-45 0.05159672369637747 1106 46-47 0.014853834797108334 1106 48-49 0.08508641353340352 1106 50-51 0.04311155077285633 1106 52-53 0.03807439529018097 1106 54-55 -0.00686685408903287 1106 56-57 0.07562978435128542 1106 58-59 0.09206556049738168 1106 60-61 0.07654472974284232 1106 62-63 0.0606811715214306 1106 64-65 0.0053070117837847874 1106 66-67 0.025994287423451112 1106 68-69 0.06927011033403119 1106 70-71 0.05619419525142888 1106 72-73 0.13121136293121793 1106 74-75 0.01886001608254162 1106 76-77 -0.005093957771315161 1106 78-79 0.0492545622328322 1106 80-81 0.040474629474339 1106 82-83 0.0030272121521903728 1106 84-85 0.013979230495202444 1106 86-87 0.005819854969431049 1106 88-89 -0.04310842146096405 1106 90-91 0.027424464613325483 1106 92-93 -0.07438652606489171 1106 94-95 -0.12237194835343246 1106 96-97 -0.08314093239767573 1106 98-99 -0.008785250121235322 1106 100 0.025324340919283372 1107 1 -0.026852396648351373 1107 2 0.024250812789752274 1107 3 0.03141106307729302 1107 4 0.06468737199475783 1107 5 -0.08725050370832577 1107 6 -0.0821247268511982 1107 7 -0.04327677641672523 1107 8 -0.03673463606289573 1107 9 0.007168041791423718 1107 10-11 0.007297900569760429 1107 12-13 0.03461643305970341 1107 14-15 0.032575367129439314 1107 16-17 0.05379847061094978 1107 18-19 0.007423531456552723 1107 20-21 0.06100811178839649 1107 22-23 -0.026920458590808494 1107 24-25 0.006049667979119988 1107 26-27 0.037842864209835625 1107 28-29 0.03502503534434709 1107 30-31 0.04067308804203407 1107 32-33 0.02498283246613653 1107 34-35 0.066289528611712 1107 36-37 -0.016681869425831053 1107 38-39 0.004833090372677873 1107 40-41 0.09832546096688333 1107 42-43 0.05682620907825964 1107 44-45 0.09512747170560942 1107 46-47 0.09310831790732266 1107 48-49 0.15562028784296444 1107 50-51 0.15693521034987867 1107 52-53 0.24613082200715297 1107 54-55 0.17207013367589497 1107 56-57 0.16457914058076284 1107 58-59 0.18508817513755815 1107 60-61 0.20566259096263906 1107 62-63 0.15643127084684494 1107 64-65 0.1459800086096692 1107 66-67 0.15080478026715838 1107 68-69 0.12335198252528556 1107 70-71 0.20009166241745646 1107 72-73 0.21340149114029572 1107 74-75 0.2606861279973174 1107 76-77 0.2292223080584037 1107 78-79 0.2284471744936596 1107 80-81 0.2358625037893134 1107 82-83 0.24210488263532426 1107 84-85 0.1872725584886865 1107 86-87 0.14497368689058732 1107 88-89 0.1900177720974554 1107 90-91 0.2675585782282113 1107 92-93 0.2728136924589961 1107 94-95 0.26095005133772275 1107 96-97 0.2991979329323655 1107 98-99 0.2954529211500514 1107 100 0.27487281467052327 1108 1 0.002659519684073075 1108 2 -0.003924708421450873 1108 3 -0.017453771079665614 1108 4 0.00308828368658709 1108 5 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-0.020255039099282612 2214 34-35 -0.05813870990532877 2214 36-37 -0.13090499504516373 2214 38-39 -0.12733881535751124 2214 40-41 0.01933260059880837 2214 42-43 0.011671067543240099 2214 44-45 0.046271752272538436 2214 46-47 0.06224168295889143 2214 48-49 -0.04670130469497025 2214 50-51 0.11848112551674461 2214 52-53 -0.03585357747283524 2214 54-55 -0.06868864919263018 2214 56-57 -0.05577449241913257 2214 58-59 -0.12684404078478906 2214 60-61 -0.19385496871358754 2214 62-63 -0.18395363592276937 2214 64-65 -0.09070128985786141 2214 66-67 -0.22163664959868612 2214 68-69 -0.20446955091976093 2214 70-71 -0.08762224622797987 2214 72-73 -0.2727858514680932 2214 74-75 -0.22229133488781372 2214 76-77 -0.19912606112966813 2214 78-79 -0.43591694067381326 2214 80-81 -0.381594820545736 2214 82-83 -0.31910980936956435 2214 84-85 -0.3738909954479652 2214 86-87 -0.33340210745407006 2214 88-89 -0.3436865331975696 2214 90-91 -0.1878383440934428 2214 92-93 -0.1449282625758741 2214 94-95 -0.18929033546304908 2214 96-97 -0.3253928867616267 2214 98-99 -0.309425953600325 2214 100 -0.4505068191960646 2215 1 -0.06110607250689526 2215 2 -0.0949471159687576 2215 3 -0.06704853769497987 2215 4 -0.08708424907410972 2215 5 -0.038693088451374535 2215 6 -0.11671150679234188 2215 7 -0.067586896166965 2215 8 -0.09963209921392036 2215 9 -0.149902359779027 2215 10-11 -0.1280045785773396 2215 12-13 -0.11910334218919871 2215 14-15 -0.1614706422128407 2215 16-17 -0.18396688460590838 2215 18-19 -0.23020834107501997 2215 20-21 -0.2435678885972834 2215 22-23 -0.23846776547217274 2215 24-25 -0.15569865388289372 2215 26-27 -0.16899074118612134 2215 28-29 -0.15504059866451314 2215 30-31 -0.18068836001911137 2215 32-33 -0.25707587430359524 2215 34-35 -0.2219935993124551 2215 36-37 -0.20246988054135073 2215 38-39 -0.22559953062613403 2215 40-41 -0.1532085729747834 2215 42-43 -0.1685858961139317 2215 44-45 -0.18499626342918418 2215 46-47 -0.16942756686366778 2215 48-49 -0.16496542393537794 2215 50-51 -0.11967051780495552 2215 52-53 -0.13062232466636914 2215 54-55 -0.0879066047816579 2215 56-57 -0.17886826822051205 2215 58-59 -0.10871173491981523 2215 60-61 -0.13826014275070264 2215 62-63 -0.24663777631126038 2215 64-65 -0.17410258191863193 2215 66-67 -0.17266037600627016 2215 68-69 -0.06437800580627595 2215 70-71 -0.055017628414340436 2215 72-73 0.012362329941872474 2215 74-75 0.04571060610035005 2215 76-77 -0.08892500951965587 2215 78-79 -0.006944795302494811 2215 80-81 -0.03287565546034443 2215 82-83 -0.09358328011713013 2215 84-85 -0.06330826444863646 2215 86-87 -0.07452570784226964 2215 88-89 -0.12853451326622434 2215 90-91 -0.028697955606112657 2215 92-93 -0.020442117465279352 2215 94-95 -0.027377520595663896 2215 96-97 -0.004283916209601557 2215 98-99 -0.0893472276997187 2215 100 0.00564712723846128 2216 1 -0.19233481834915267 2216 2 -0.14651305581763552 2216 3 -0.22444181190106605 2216 4 -0.12455281666832008 2216 5 -0.12699776373914062 2216 6 -0.10308437624706812 2216 7 -0.1562770055126066 2216 8 -0.11171369390173425 2216 9 -0.20285166744918115 2216 10-11 -0.18680576618843503 2216 12-13 -0.16325157623703745 2216 14-15 -0.27029550149369186 2216 16-17 -0.26985454182493385 2216 18-19 -0.24827807029087978 2216 20-21 -0.2660454879024172 2216 22-23 -0.28499084174054445 2216 24-25 -0.3508318649695923 2216 26-27 -0.38742903412056506 2216 28-29 -0.3642261184419411 2216 30-31 -0.2519251914654248 2216 32-33 -0.22246257363691058 2216 34-35 -0.16618332527583135 2216 36-37 -0.26935013684892084 2216 38-39 -0.3006190417054668 2216 40-41 -0.30798132114544785 2216 42-43 -0.31751907513179134 2216 44-45 -0.06906344385594565 2216 46-47 -0.17869645043477078 2216 48-49 -0.2075211587488397 2216 50-51 -0.192194662528145 2216 52-53 -0.08296278027192017 2216 54-55 -0.06218389692502768 2216 56-57 -0.055379132491033545 2216 58-59 -0.0789404496051489 2216 60-61 -0.10592441387984763 2216 62-63 0.06342971264512443 2216 64-65 0.030624988699315736 2216 66-67 -0.010114960976920884 2216 68-69 -0.015826385227803996 2216 70-71 0.0077770420782954375 2216 72-73 0.10426791075649788 2216 74-75 0.12095338320136761 2216 76-77 0.03338730605599238 2216 78-79 0.0066937341206738665 2216 80-81 0.07219973291842052 2216 82-83 -0.034869407910711914 2216 84-85 0.0936055729532086 2216 86-87 0.13266022077491613 2216 88-89 0.02608131035209027 2216 90-91 -0.039078381756958436 2216 92-93 -0.043955950763532314 2216 94-95 -0.11474095253942096 2216 96-97 -0.15398781135817075 2216 98-99 -0.10519729141945078 2216 100 -0.1760648170377479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 6.0 4 7.0 5 16.0 6 32.0 7 70.0 8 148.0 9 296.0 10 559.0 11 810.0 12 1319.0 13 2057.0 14 2420.0 15 3388.0 16 4347.0 17 6358.0 18 7427.0 19 4132.0 20 2792.0 21 2716.0 22 2965.0 23 3092.0 24 3577.0 25 4201.0 26 5387.0 27 6963.0 28 9222.0 29 11867.0 30 15400.0 31 19500.0 32 25223.0 33 33207.0 34 46157.0 35 72823.0 36 139267.0 37 319612.0 38 561792.0 39 271393.0 40 1083.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29222853254666 19.496554755707436 13.55930178227012 23.651914929475783 2 15.702448703143594 19.78408135620482 40.39398900992575 24.11948093072584 3 18.585912275444024 25.06117899702257 29.869235587673348 26.48367313986006 4 12.487151584910118 16.451751531128977 38.19277774566547 32.86831913829544 5 12.813110838754282 37.76707205933189 35.366456113887054 14.053360988026775 6 31.462450294885073 37.62509919033046 17.354208277389883 13.558242237394582 7 27.86728374893816 32.33417694159751 21.93900888176248 17.85953042770185 8 25.66372126220131 36.466909436352125 19.645495943786003 18.223873357660565 9 25.353724092694563 16.55937664139288 20.87795199404889 37.20894727186367 10-11 24.540857501607626 26.578070167447258 28.226500351368138 20.654571979576982 12-13 25.171819524628987 23.688978858926102 28.90111526405766 22.238086352387253 14-15 22.225908152481907 25.59551857897336 26.157078431150804 26.02149483739393 16-17 21.66876827123704 29.21173807410088 26.64573858049648 22.473755074165595 18-19 21.49910736752426 27.714329963330286 29.32640882159005 21.4601538475554 20-21 22.471524671706746 26.985922076649192 29.13543317871304 21.40712007293102 22-23 22.124911569087946 26.754481860825134 29.213677984566843 21.906928585520074 24-25 21.967875901585785 26.86537156643462 29.162310572742577 22.004441959237013 26-27 21.820423103770437 26.858626342108256 29.237116420449873 22.083834133671438 28-29 21.819404793658304 26.93973635539085 29.223433135500915 22.01742571544994 30-31 21.89145235622332 27.243884158442288 28.982136038879926 21.882527446454468 32-33 21.779981127611098 27.01111447332492 29.275832564266203 21.933071834797783 34-35 21.911094784863806 26.80737685213961 29.245022441083318 22.036505921913264 36-37 22.217061926792166 27.07079111832854 28.856351096445813 21.855795858433474 38-39 21.994738648061308 27.21690696356143 28.901771338577927 21.886583049799338 40-41 21.853055986243678 27.081375858434576 29.00762543184908 22.057942723472667 42-43 21.919572565271366 27.074196839549526 28.86230758571585 22.143923009463254 44-45 21.91763561781142 27.298892559932003 28.739711521995627 22.043760300260953 46-47 22.125884056969245 27.441819547124048 28.44016375672763 21.99213263917908 48-49 22.154956986065006 27.39646149041667 28.18972629552342 22.258855227994907 50-51 22.147506363449576 27.431887055760217 28.18321708112605 22.237389499664157 52-53 22.10941105470553 27.466772140465718 28.13891628184752 22.284900522981236 54-55 22.158958464361987 27.687074359768605 28.07759995928556 22.076367216583844 56-57 21.99308871575773 27.546965317919074 28.10828725810505 22.351658708218146 58-59 21.937209436019614 27.23506706512748 28.345756175530095 22.481967323322806 60-61 22.1912372249715 27.46435180778453 28.13432514644574 22.21008582079823 62-63 21.978930239206342 27.471518753720662 28.220819386567435 22.328731620505554 64-65 22.006069383228773 27.350393787364247 28.25721995859499 22.386316870811985 66-67 21.873020899303356 27.358145601495824 28.194676910240556 22.57415658896026 68-69 22.189931673604022 27.450530118589494 28.115636534987825 22.243901672818662 70-71 22.207408668630475 27.28024098482347 28.089314419036267 22.423035927509787 72-73 22.099811294497133 27.456634960246816 28.09333932717551 22.350214418080544 74-75 22.127546482964178 27.448595984686154 28.005728712958998 22.41812881939067 76-77 22.115616295313846 27.43844303548143 27.89479752116612 22.551143148038605 78-79 22.194574272181068 27.588322477647253 27.938119133798555 22.27898411637312 80-81 22.10025074938946 27.498022466290827 27.97815948314588 22.42356730117383 82-83 22.019069710750085 27.509119558893563 28.082461168836552 22.389349561519797 84-85 21.97930996135736 27.428484725302997 27.937489456599828 22.654715856739813 86-87 22.0972042637836 27.502579104042503 27.92007353430868 22.48014309786522 88-89 21.860244194590425 27.479100965324772 27.963067036808877 22.69758780327593 90-91 21.90431133653883 27.3770278599257 27.973958951798807 22.744701851736668 92-93 22.01121358396774 27.470139309052733 27.85923458870436 22.65941251827516 94-95 21.85593359306527 27.300471651584747 28.03499658528525 22.80859817006473 96-97 21.832749973140928 27.307853969582336 28.007949028706854 22.851447028569886 98-99 21.828634723533764 27.36402241460229 27.96261845478974 22.844724407074207 100 21.938112928732046 27.269528744233153 27.796594835141846 22.99576349189296 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 21.0 1 19.5 2 90.0 3 173.0 4 130.5 5 88.0 6 206.0 7 493.5 8 896.5 9 1064.5 10 824.5 11 602.5 12 611.5 13 717.5 14 803.0 15 910.0 16 1031.0 17 1162.0 18 1310.0 19 1524.0 20 1838.5 21 2204.0 22 2804.5 23 3818.0 24 5134.0 25 6677.0 26 8891.5 27 11917.5 28 15469.5 29 19474.0 30 23332.0 31 27176.0 32 31473.5 33 35726.5 34 39715.5 35 43374.0 36 46080.5 37 48800.0 38 51467.5 39 52948.5 40 54645.5 41 56297.0 42 56478.0 43 56379.0 44 56046.5 45 55071.5 46 54671.5 47 53578.0 48 52166.5 49 51463.0 50 50413.0 51 49265.0 52 47127.0 53 45398.0 54 43921.5 55 41361.0 56 38456.0 57 34734.5 58 31401.5 59 29359.5 60 26805.5 61 22819.5 62 19539.5 63 16707.0 64 13807.5 65 11140.5 66 8538.0 67 6333.5 68 4692.0 69 3593.5 70 2543.5 71 1770.0 72 1332.0 73 947.5 74 654.0 75 454.5 76 285.5 77 176.0 78 122.5 79 73.0 80 36.0 81 19.5 82 13.0 83 6.5 84 2.0 85 2.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09286013617392365 2 0.0014450494803790554 3 0.0 4 3.1414119138675116E-4 5 0.037131488821913985 6 2.5131295310940093E-4 7 0.004083835488027765 8 5.654541444961521E-4 9 3.1414119138675116E-4 10-11 3.455553105254263E-4 12-13 0.0 14-15 1.884847148320507E-4 16-17 0.0 18-19 3.1414119138675116E-5 20-21 0.0 22-23 1.884847148320507E-4 24-25 6.282823827735023E-5 26-27 0.001382221242101705 28-29 0.008953023954522408 30-31 0.036974418226220614 32-33 0.026859071863567226 34-35 0.029811999062602686 36-37 0.027110384816676623 38-39 0.026073718885100343 40-41 0.03254502742766742 42-43 0.023906144664531764 44-45 0.022523923422430057 46-47 0.01608402899900166 48-49 0.011309082889923041 50-51 0.00816767097605553 52-53 0.005811612040654897 54-55 0.004272320202859816 56-57 0.0025759577693713594 58-59 0.0010366659315762788 60-61 6.596965019121775E-4 62-63 0.0014764635995177303 64-65 0.002890098960758111 66-67 5.654541444961521E-4 68-69 0.0010052518124376037 70-71 3.455553105254263E-4 72-73 4.712117870801268E-4 74-75 5.968682636348273E-4 76-77 3.455553105254263E-4 78-79 1.2565647655470046E-4 80-81 7.539388593282028E-4 82-83 4.397976679414517E-4 84-85 3.455553105254263E-4 86-87 1.884847148320507E-4 88-89 3.1414119138675116E-5 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1591641.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.54139980268874 #Duplication Level Percentage of deduplicated Percentage of total 1 79.90904160218834 53.97168526703089 2 12.55596165741224 16.96094452421021 3 3.489365556987663 7.0702990242670625 4 1.4647982443238026 3.957380954006019 5 0.7434690120442525 2.510746889169544 6 0.46046507131411324 1.8660273286080058 7 0.2833875234166042 1.3398273012722275 8 0.20579688644407143 1.1119847828374048 9 0.1482124750219124 0.9009430228080904 >10 0.7066559083684222 7.995725127666247 >50 0.026603446777576204 1.1836290351623864 >100 0.005869988554972298 0.6480891243331223 >500 9.315678652692713E-5 0.050587195695717256 >1k 2.7947035958078136E-4 0.4321304229330673 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2979 0.18716532182822634 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2534 0.15920675579480548 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.1414119138675116E-5 0.0 22-23 0.0 0.0 0.0 9.424235741602535E-5 0.0 24-25 0.0 0.0 0.0 2.8272707224807604E-4 0.0 26-27 0.0 0.0 0.0 4.7121178708012674E-4 0.0 28-29 0.0 0.0 0.0 0.0010366659315762788 0.0 30-31 0.0 0.0 0.0 0.003549795462670288 0.0 32-33 0.0 0.0 0.0 0.009361407503325186 0.0 34-35 0.0 0.0 0.0 0.01690079609660721 0.0 36-37 0.0 0.0 0.0 0.031099977947288367 0.0 38-39 0.0 0.0 0.0 0.05723652507066606 0.0 40-41 0.0 0.0 0.0 0.09031559252369095 0.0 42-43 0.0 0.0 0.0 0.1220752669728915 0.0 44-45 0.0 0.0 0.0 0.1582957463397839 0.0 46-47 0.0 0.0 0.0 0.2052598544521032 0.0 48-49 0.0 0.0 0.0 0.25875809934526695 0.0 50-51 0.0 0.0 0.0 0.31721977506234134 0.0 52-53 0.0 0.0 0.0 0.3786657920975899 0.0 54-55 0.0 0.0 0.0 0.44139978801752405 0.0 56-57 0.0 0.0 0.0 0.5136208479173381 0.0 58-59 0.0 0.0 0.0 0.5983761413534836 0.0 60-61 0.0 0.0 0.0 0.6907336516211884 0.0 62-63 0.0 0.0 0.0 0.7841592419396082 0.0 64-65 0.0 0.0 0.0 0.8830194748690189 0.0 66-67 0.0 0.0 0.0 0.996612929674468 0.0 68-69 0.0 0.0 0.0 1.1264160699554737 0.0 70-71 0.0 0.0 0.0 1.2721147545206488 0.0 72-73 0.0 0.0 0.0 1.4312900961963155 0.0 74-75 0.0 0.0 0.0 1.5855020070480719 0.0 76-77 0.0 0.0 0.0 1.7424783603840313 0.0 78-79 0.0 0.0 0.0 1.9329421647218186 0.0 80-81 0.0 0.0 0.0 2.1391758568672206 0.0 82-83 0.0 0.0 0.0 2.3633155969216677 0.0 84-85 0.0 0.0 0.0 2.5730676704106017 0.0 86-87 0.0 0.0 0.0 2.7966419563205522 0.0 88 0.0 0.0 0.0 2.983587379314808 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2460 0.0 44.556904 1 GTATCAA 4105 0.0 43.203842 1 TCAACGC 4710 0.0 36.92844 4 ATCAACG 4725 0.0 36.9107 3 CAACGCA 4800 0.0 36.234898 5 AACGCAG 5100 0.0 34.373466 6 TATCAAC 5130 0.0 34.088333 2 TATACCG 85 1.7533053E-5 33.181717 5 ACGCAGA 5930 0.0 29.324572 7 CGCAGAG 6105 0.0 28.404322 8 TCGCGCA 70 0.008842399 26.853796 9 GTACATG 4675 0.0 26.46478 1 GCAGAGT 6650 0.0 25.793777 9 TACATGG 4710 0.0 25.350876 2 GTACGAC 175 1.6439844E-7 24.176012 3 ACATGGG 4750 0.0 23.751871 3 GTGGTAT 1380 0.0 23.180565 1 TATAACG 125 2.4208934E-4 22.564278 2 GTATAGA 715 0.0 22.370054 1 AATACCG 170 3.448482E-6 22.121145 5 >>END_MODULE