##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033142_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	833691
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.40044812766361	33.0	31.0	34.0	26.0	34.0
2	30.518304743604045	33.0	31.0	34.0	26.0	34.0
3	30.705425631319038	34.0	31.0	34.0	26.0	34.0
4	33.7538620424114	37.0	35.0	37.0	28.0	37.0
5	34.18426491349913	37.0	35.0	37.0	31.0	37.0
6	34.17302813632389	37.0	35.0	37.0	30.0	37.0
7	34.257293169771536	37.0	35.0	37.0	31.0	37.0
8	34.1516581083399	37.0	35.0	37.0	30.0	37.0
9	35.5623186528342	39.0	37.0	39.0	28.0	39.0
10-11	35.63265046641981	39.0	37.0	39.0	30.0	39.0
12-13	35.59190035636705	39.0	37.0	39.0	30.0	39.0
14-15	36.84186886988105	40.0	38.0	41.0	29.5	41.0
16-17	36.96887635826703	40.0	38.0	41.0	30.0	41.0
18-19	37.0077738634578	40.0	38.0	41.0	30.0	41.0
20-21	37.03333369317889	40.0	38.0	41.0	30.0	41.0
22-23	37.04209353345544	40.0	38.0	41.0	30.0	41.0
24-25	37.04738746130161	40.0	38.0	41.0	30.0	41.0
26-27	36.961226041782865	40.0	37.5	41.0	30.0	41.0
28-29	36.941851357397404	40.0	37.5	41.0	30.0	41.0
30-31	36.92670125981928	40.0	38.0	41.0	30.0	41.0
32-33	36.928558662622	40.0	38.0	41.0	30.0	41.0
34-35	36.76032966650713	40.0	37.0	41.0	30.0	41.0
36-37	36.56962891526957	40.0	37.0	41.0	29.5	41.0
38-39	36.43016837173485	40.0	37.0	41.0	29.0	41.0
40-41	36.39715793981223	40.0	37.0	41.0	29.0	41.0
42-43	36.19717377301662	40.0	36.0	41.0	28.0	41.0
44-45	36.03323893384959	40.0	35.5	41.0	28.0	41.0
46-47	35.792815323663085	40.0	35.0	41.0	27.0	41.0
48-49	35.558036490738175	40.0	35.0	41.0	27.0	41.0
50-51	35.35894953885793	39.0	35.0	41.0	27.0	41.0
52-53	35.25857541942998	39.0	35.0	41.0	27.0	41.0
54-55	35.014627122039215	39.0	35.0	41.0	26.5	41.0
56-57	34.665879804387956	38.5	34.0	41.0	26.0	41.0
58-59	34.38431865043523	38.0	34.0	40.0	26.0	41.0
60-61	33.9125695251598	37.0	33.5	40.0	24.0	41.0
62-63	33.729499898643496	37.0	33.5	40.0	24.5	41.0
64-65	33.41005960241864	36.0	33.0	39.5	23.0	41.0
66-67	32.96342469811957	36.0	33.0	39.0	22.0	41.0
68-69	32.48631207485747	35.0	32.5	38.5	21.0	40.5
70-71	31.939851815600743	35.0	31.5	37.5	19.0	40.0
72-73	31.595570781020786	35.0	31.5	37.0	18.5	39.0
74-75	31.316405598717033	35.0	31.0	36.5	17.0	39.0
76-77	31.02560660964314	35.0	31.0	36.0	14.0	38.5
78-79	30.64106545470684	35.0	31.0	35.5	8.5	37.0
80-81	30.210606807558197	34.5	30.0	35.0	7.0	37.0
82-83	30.00362304498909	34.5	30.0	35.0	4.0	36.5
84-85	29.869801281290073	34.0	30.0	35.0	2.0	36.0
86-87	29.83682623418029	34.5	30.5	35.0	2.0	36.0
88-89	29.49393900138061	34.0	30.0	35.0	2.0	35.5
90-91	29.23539236959497	34.0	30.0	35.0	2.0	35.0
92-93	28.932476181223016	34.0	29.0	35.0	2.0	35.0
94-95	28.715399350598723	34.0	29.0	35.0	2.0	35.0
96-97	28.53356339459104	34.0	29.0	35.0	2.0	35.0
98-99	28.326092041295873	34.0	29.0	35.0	2.0	35.0
100	28.10325528283261	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.25127119319101965
1101	2	0.14649649537480514
1101	3	0.16780442155898356
1101	4	0.21822023614631547
1101	5	0.2121257235840872
1101	6	0.13766308828968477
1101	7	0.35210293043283514
1101	8	0.2792723429339645
1101	9	0.4052922863081534
1101	10-11	0.4273687334378806
1101	12-13	0.35313462770680815
1101	14-15	0.3534878918437059
1101	16-17	0.19983312362689531
1101	18-19	0.3458255777698227
1101	20-21	0.3360065574681954
1101	22-23	0.2516274664332059
1101	24-25	0.14935161696467247
1101	26-27	0.25599627049304274
1101	28-29	0.3976471329040123
1101	30-31	0.34847225965170736
1101	32-33	0.2413987091135894
1101	34-35	0.38555969550912295
1101	36-37	0.314712474277691
1101	38-39	0.30525498806277085
1101	40-41	0.3453773491604437
1101	42-43	0.3220084978004536
1101	44-45	0.2947480601310488
1101	46-47	0.3267748523712939
1101	48-49	0.18627869023217158
1101	50-51	0.2661234707123157
1101	52-53	0.27283816845887543
1101	54-55	0.2285390595665504
1101	56-57	0.27724490654527045
1101	58-59	0.3573181109374204
1101	60-61	0.3820282008514013
1101	62-63	0.12384519897927504
1101	64-65	0.256759961947175
1101	66-67	0.24326734569972785
1101	68-69	0.21194915857135044
1101	70-71	0.2363779966639754
1101	72-73	0.35984922848139433
1101	74-75	0.1781216941431154
1101	76-77	0.35288216309266573
1101	78-79	0.24846845267323658
1101	80-81	0.26609914938339685
1101	82-83	0.30772058476064856
1101	84-85	0.15703634910043718
1101	86-87	0.4147876901676959
1101	88-89	0.6089854936546217
1101	90-91	0.5840946825813198
1101	92-93	0.4960756901415486
1101	94-95	0.3473969549272269
1101	96-97	0.465239619055982
1101	98-99	0.6289111306034982
1101	100	0.6935149837296173
1102	1	0.7761868657537505
1102	2	0.7351883917137592
1102	3	0.7692030229575835
1102	4	0.9236418733326559
1102	5	0.7177365833125933
1102	6	0.744610846413913
1102	7	0.7458997218766896
1102	8	0.8204529270557259
1102	9	1.0198624220547572
1102	10-11	0.9264308477941157
1102	12-13	1.0930708679371648
1102	14-15	1.1895882620617257
1102	16-17	1.0703102935158313
1102	18-19	1.1425943971857038
1102	20-21	1.009198659483829
1102	22-23	1.0176681904151081
1102	24-25	0.9185535914607641
1102	26-27	1.022187138448615
1102	28-29	0.9776553599710667
1102	30-31	0.8832377882407627
1102	32-33	0.9870465906438213
1102	34-35	0.9751956477921411
1102	36-37	0.9926845022497162
1102	38-39	0.9721718946855589
1102	40-41	0.9586784598144931
1102	42-43	1.0098015870641248
1102	44-45	0.9034337861697068
1102	46-47	0.9258883858965916
1102	48-49	0.9877389946336592
1102	50-51	1.0079725040319403
1102	52-53	0.9901849393350517
1102	54-55	1.036901873223485
1102	56-57	1.067450583221536
1102	58-59	0.8474744252113808
1102	60-61	0.8843729149608279
1102	62-63	1.1168912705547598
1102	64-65	1.188016646439145
1102	66-67	1.0761283082665756
1102	68-69	1.0529261227836102
1102	70-71	1.1526490785232966
1102	72-73	1.2370417418503834
1102	74-75	1.3845922823784065
1102	76-77	1.3174399582386975
1102	78-79	1.3626951083704775
1102	80-81	1.3256425471620865
1102	82-83	1.2125395892855373
1102	84-85	1.2346175913875648
1102	86-87	1.2810649423273048
1102	88-89	1.4609949547817322
1102	90-91	1.4562337200144775
1102	92-93	1.461995064884455
1102	94-95	1.7139415867659764
1102	96-97	1.6347398247326588
1102	98-99	1.6049765357865482
1102	100	1.5761517587193339
1103	1	0.22017287973976352
1103	2	0.17553804206340828
1103	3	0.16658064533520545
1103	4	0.21759292228370697
1103	5	0.13962469520070897
1103	6	0.2164989583020258
1103	7	0.28324237921934525
1103	8	0.13772565432845596
1103	9	0.26000228219461974
1103	10-11	0.26333644219970864
1103	12-13	0.38464429451059345
1103	14-15	0.49383651380997406
1103	16-17	0.3659221816277167
1103	18-19	0.42565383774514487
1103	20-21	0.3174853727705411
1103	22-23	0.3671611974081159
1103	24-25	0.42448016488733487
1103	26-27	0.24138294264442095
1103	28-29	0.3498581571738626
1103	30-31	0.37986366236663827
1103	32-33	0.46040323400047356
1103	34-35	0.5406501932466838
1103	36-37	0.4356040826692933
1103	38-39	0.42802853804220575
1103	40-41	0.5138882500242872
1103	42-43	0.455780608043149
1103	44-45	0.41885336658928907
1103	46-47	0.3831611131693222
1103	48-49	0.4659732603679245
1103	50-51	0.48590956696899923
1103	52-53	0.45375137290149326
1103	54-55	0.42980397112558677
1103	56-57	0.3163491846201367
1103	58-59	0.4001142853512434
1103	60-61	0.5203869009748132
1103	62-63	0.610877284540777
1103	64-65	0.5746949681174769
1103	66-67	0.6695373991756668
1103	68-69	0.5014750738325588
1103	70-71	0.5065651624393723
1103	72-73	0.4077410425496879
1103	74-75	0.5052041704902948
1103	76-77	0.5035763218750589
1103	78-79	0.4651076957830682
1103	80-81	0.5555376520571933
1103	82-83	0.6329416871876354
1103	84-85	0.5490406683106421
1103	86-87	0.6212048024671617
1103	88-89	0.7454005491873268
1103	90-91	0.7870204333011905
1103	92-93	0.8077118481012349
1103	94-95	0.7330150133394042
1103	96-97	0.7843726918655243
1103	98-99	0.8699590532690671
1103	100	0.8952426678102441
1104	1	0.11205295911984336
1104	2	0.12938065215601924
1104	3	0.05318643819099833
1104	4	0.08103437947516312
1104	5	0.11979739412340962
1104	6	0.12250089555396215
1104	7	0.18893719116415753
1104	8	0.17596275506555514
1104	9	0.11523404367638079
1104	10-11	0.13860222371548758
1104	12-13	0.08591177702807329
1104	14-15	0.14392333577179528
1104	16-17	0.13801453184507295
1104	18-19	0.06310422519553072
1104	20-21	0.17360897889415128
1104	22-23	0.08694183906375486
1104	24-25	0.0887685791757562
1104	26-27	0.14898842837489923
1104	28-29	0.16728988085558427
1104	30-31	0.0710019160048887
1104	32-33	0.20367896165119248
1104	34-35	0.18972113919263478
1104	36-37	0.29245415826937204
1104	38-39	0.15878890942757806
1104	40-41	0.07825462689066143
1104	42-43	0.06943764357518489
1104	44-45	0.044361304597224205
1104	46-47	0.046206260589471526
1104	48-49	0.09643064082530373
1104	50-51	0.10409965390908837
1104	52-53	0.12572087299598422
1104	54-55	-0.0525806206340107
1104	56-57	-0.01842625953030108
1104	58-59	0.12934788958484233
1104	60-61	0.1486235292114415
1104	62-63	0.19889526318375061
1104	64-65	0.17792450884700628
1104	66-67	0.23733531072357295
1104	68-69	0.27243932917181013
1104	70-71	0.1442399876141991
1104	72-73	0.25258074676438724
1104	74-75	0.1960618769730047
1104	76-77	0.1491537174524531
1104	78-79	0.08346906414443467
1104	80-81	0.28606177320631687
1104	82-83	0.3779449946909388
1104	84-85	0.30757520809518013
1104	86-87	0.29097126910863125
1104	88-89	0.5451135051502831
1104	90-91	0.648557122962881
1104	92-93	0.5741341926485788
1104	94-95	0.7460914639158567
1104	96-97	0.6556842350520675
1104	98-99	0.6748429362779476
1104	100	0.7708569184244922
1105	1	0.10550821708494595
1105	2	0.2538035009687363
1105	3	0.2707765929478896
1105	4	0.19592765154132508
1105	5	-0.09346932316812939
1105	6	0.10776171523335876
1105	7	0.12369970696133237
1105	8	0.13783751949364387
1105	9	0.09059252869955259
1105	10-11	0.11795295980842724
1105	12-13	0.10957358998952316
1105	14-15	0.2639905291957447
1105	16-17	0.3225833936724456
1105	18-19	0.3159962168590553
1105	20-21	0.19707471288079859
1105	22-23	0.23029739468090327
1105	24-25	0.26166866342735773
1105	26-27	0.29794297408139414
1105	28-29	0.3297174078493583
1105	30-31	0.36223808654786893
1105	32-33	0.47138323021426487
1105	34-35	0.24645413090049573
1105	36-37	0.3625070095209466
1105	38-39	0.4585150224355772
1105	40-41	0.35342340723786947
1105	42-43	0.2973565874370152
1105	44-45	0.43680055475797275
1105	46-47	0.46962356376219816
1105	48-49	0.45032755455609674
1105	50-51	0.49945098863183546
1105	52-53	0.5466224067077192
1105	54-55	0.5421244849022102
1105	56-57	0.551520237931058
1105	58-59	0.4700167475322061
1105	60-61	0.5174626308233101
1105	62-63	0.5946446451539131
1105	64-65	0.638615870840674
1105	66-67	0.6275553348904808
1105	68-69	0.6332762607506481
1105	70-71	0.5395947866297917
1105	72-73	0.4899655243845018
1105	74-75	0.5632059000978558
1105	76-77	0.5794535312120708
1105	78-79	0.6017051016585704
1105	80-81	0.6222530326569036
1105	82-83	0.5255543554873405
1105	84-85	0.42763720507986136
1105	86-87	0.5563516900340666
1105	88-89	0.6511534304324158
1105	90-91	0.7833177680419219
1105	92-93	0.5820823197133009
1105	94-95	0.3709085492538584
1105	96-97	0.5007891199821195
1105	98-99	0.6544891765502143
1105	100	0.7741299823005114
1106	1	0.07782322939011266
1106	2	0.21107930080466986
1106	3	0.272112113866676
1106	4	0.21266005515084174
1106	5	0.12253658331259487
1106	6	0.12275630095936663
1106	7	0.11280881278577937
1106	8	0.27394383614663553
1106	9	0.0654624220547575
1106	10-11	0.3493035750668412
1106	12-13	0.18836177702807078
1106	14-15	0.18180644387990696
1106	16-17	0.1472557480612835
1106	18-19	0.1610853062766111
1106	20-21	0.12534411402927503
1106	22-23	0.27186819041510546
1106	24-25	0.21091722782440314
1106	26-27	0.2656053202667934
1106	28-29	0.2586371781528811
1106	30-31	0.28965597005894494
1106	32-33	0.30846477246200976
1106	34-35	0.1292501932466834
1106	36-37	0.07686632043153452
1106	38-39	0.19113553104919845
1106	40-41	0.13993300526904306
1106	42-43	0.13912885979140555
1106	44-45	0.18468833162425113
1106	46-47	0.18539747680568297
1106	48-49	0.08181172190638364
1106	50-51	0.01653614039557283
1106	52-53	-0.0506514243013072
1106	54-55	0.027920055041668945
1106	56-57	0.07479603776698696
1106	58-59	0.02852897066592419
1106	60-61	-0.026208903220997115
1106	62-63	-0.04189054762705524
1106	64-65	-0.026110626288122774
1106	66-67	-0.058144419006154635
1106	68-69	-0.044582968125482125
1106	70-71	-0.03726001238580068
1106	72-73	0.03069628730492724
1106	74-75	0.03019228237840821
1106	76-77	-0.03543276903403125
1106	78-79	-0.05499580072043031
1106	80-81	-0.012648361928821572
1106	82-83	0.04793958928553721
1106	84-85	0.11156304593301769
1106	86-87	-0.06620778494542634
1106	88-89	0.15410404569082203
1106	90-91	0.05510644728720493
1106	92-93	0.03261324670263477
1106	94-95	-0.008422049597658798
1106	96-97	-0.07360562981279628
1106	98-99	-0.27347800966799696
1106	100	-0.1337755140079402
1107	1	-0.06081440669075633
1107	2	0.006596563635149266
1107	3	-0.01360483792181455
1107	4	-0.19366902727528412
1107	5	-0.0029788132659405164
1107	6	-0.04154354351963718
1107	7	0.049623742801330195
1107	8	-0.08014512186269229
1107	9	-0.15534753128893897
1107	10-11	-0.01582986194716085
1107	12-13	-0.13439343292527184
1107	14-15	-0.08182839282304855
1107	16-17	-0.14938997511134744
1107	18-19	-0.12690722871560922
1107	20-21	-0.1731765702630952
1107	22-23	-0.07266617972486245
1107	24-25	-0.09452289659239455
1107	26-27	-0.14907399544082978
1107	28-29	-0.057428762749140105
1107	30-31	-0.07148368779486702
1107	32-33	-0.11505435662041918
1107	34-35	-0.09329226398504176
1107	36-37	-0.1146391228033039
1107	38-39	-0.09189215168797205
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2216	70-71	-1.1733441638584523
2216	72-73	-1.2032190002126057
2216	74-75	-1.1312422197253795
2216	76-77	-1.0698888700157987
2216	78-79	-1.0464729395703607
2216	80-81	-0.9978943647338703
2216	82-83	-1.1727970166050667
2216	84-85	-1.3190417226504323
2216	86-87	-1.4181713193072767
2216	88-89	-1.2943862768898242
2216	90-91	-1.4324262315346772
2216	92-93	-1.4301699230028362
2216	94-95	-1.3454374773676427
2216	96-97	-1.434132418031588
2216	98-99	-1.504075485123817
2216	100	-1.3083146444427207
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15711.0
3	2695.0
4	2220.0
5	2218.0
6	2710.0
7	4838.0
8	5179.0
9	3517.0
10	2802.0
11	2800.0
12	2932.0
13	2920.0
14	3039.0
15	3010.0
16	2822.0
17	2824.0
18	2758.0
19	2410.0
20	2638.0
21	2522.0
22	3280.0
23	6052.0
24	10315.0
25	11360.0
26	9509.0
27	6924.0
28	7342.0
29	8889.0
30	10929.0
31	13929.0
32	18381.0
33	25169.0
34	37826.0
35	65401.0
36	120986.0
37	190601.0
38	172156.0
39	42052.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.087882686681056	18.770829095802107	12.314215598367491	19.827072619149348
2	15.781724730098112	25.292478774123044	33.62827394891379	25.297522546865054
3	24.70570019828029	26.983763100662056	27.43973575430527	20.87080094675238
4	12.320171833470328	18.021035200662375	36.84543987328481	32.81335309258248
5	12.393695212609575	43.45251309497381	30.548338903322193	13.605452789094421
6	28.8907602541218	37.89538588510777	15.914281875587802	17.299571985182627
7	26.91693606914297	36.06998266627481	19.37466637077378	17.63841489380844
8	25.66619999976007	31.088293618659957	18.619529710126674	24.6259766714533
9	23.9613075671707	22.235792059965355	20.43949478614309	33.36340558672086
10-11	25.956665706881658	27.43089547941261	23.736203090507725	22.876235723198004
12-13	28.927125846055656	26.63284843337244	22.990640303080472	21.449385417491428
14-15	23.812543039016443	28.228076194955047	25.569734603729128	22.389646162299382
16-17	21.68466338704656	30.814012427844656	24.930491529114377	22.570832655994405
18-19	21.639747051999265	32.794881054213946	23.81411129280135	21.751260600985447
20-21	25.646790641871625	26.936772645470906	22.33137372525495	25.085062987402516
22-23	23.699419538233876	28.004505971672124	23.581131783475826	24.714942706618174
24-25	26.534015684641876	27.829531350387317	22.90488013960567	22.731572825365138
26-27	24.58960931454588	25.57996964734534	27.494826252047005	22.335594786061776
28-29	21.90099028906075	31.756672859097506	24.129605557951614	22.21273129389013
30-31	23.516592544782057	27.642684127999118	23.569259672935935	25.271463654282893
32-33	23.759339410136203	27.934636876535322	24.610451741007022	23.695571972321453
34-35	25.903001627916183	26.96222682618726	24.221821274717154	22.912950271179405
36-37	24.263724162419134	26.424298332215717	26.79137161851729	22.52060588684786
38-39	23.273575523610614	28.86831248567936	25.469627045527343	22.388484945182686
40-41	23.08071559797007	29.79632916259059	24.235012286707025	22.887942952732317
42-43	22.425563410102097	29.379676090922942	22.25660335712889	25.938157141846073
44-45	23.85741284884603	26.417621384234007	23.725518774362765	25.99944699255719
46-47	25.649565636718275	28.58394691195417	24.29369483490357	21.472792616423995
48-49	24.2721090230592	27.89541520898545	24.77435304744089	23.058122720514454
50-51	20.147065438722233	28.720464707593486	27.99491388694794	23.13755596673634
52-53	22.924776250460337	29.791347031607433	23.02266105108603	24.261215666846205
54-55	21.475837044367474	27.065089289730555	24.9268738848155	26.53219978108647
56-57	24.289646982619022	25.99733707582137	24.22175441121787	25.491261530341742
58-59	27.002454806785174	26.181986768153603	23.175643134787666	23.639915290273557
60-61	23.360476546620372	27.375700896463744	26.90590759232281	22.35791496459307
62-63	21.55709560466253	27.503711011884235	25.99921431505966	24.939979068393576
64-65	21.521541993633004	30.29343604334548	24.183853640631707	24.001168322389805
66-67	23.170858240576532	26.819695649331603	22.99489070400809	27.014555406083772
68-69	22.113484977706765	27.256882593764132	24.250384748716804	26.379247679812295
70-71	25.060065805493547	26.906002262288748	23.58364050960273	24.45029142261497
72-73	23.55980184005662	24.790684563436372	27.680796958028957	23.968716638478053
74-75	21.40261902505671	29.985401880122687	24.730318627656782	23.881660467163826
76-77	22.880499485700064	26.074077183975437	24.345189721198455	26.70023360912605
78-79	23.506945277451237	26.599393037929133	25.361898135930716	24.531763548688915
80-81	24.878907177763544	25.694289504808925	25.145563288542782	24.281240028884746
82-83	23.369628778859678	24.99083867369185	27.665046316427112	23.97448623102136
84-85	22.511287521111623	27.184693113772457	26.50510277521879	23.79891658989713
86-87	22.556275534806506	27.762677139285998	25.26779309699568	24.413254228911818
88-89	21.8868557573534	27.54497305327974	23.29879027902573	27.269380910341134
90-91	23.58581207248464	24.996891894664802	24.7204817502918	26.696814282558762
92-93	25.126704735419754	26.952411644644968	25.02477365748502	22.896109962450257
94-95	23.28550059044202	26.232762683340244	25.663983278150994	24.81775344806675
96-97	20.681835872563223	26.775685905841023	28.19567949767719	24.34679872391856
98-99	22.805263481819292	28.35119163268582	23.861375026699957	24.982169858794936
100	22.169418545434358	24.273573999029743	24.448075535270792	29.108931920265107
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4013.0
1	2981.5
2	1753.0
3	1371.0
4	941.0
5	588.5
6	467.5
7	472.5
8	548.0
9	580.5
10	480.0
11	379.5
12	341.5
13	331.5
14	338.5
15	333.0
16	345.0
17	370.0
18	411.0
19	509.0
20	650.0
21	768.0
22	866.0
23	1158.5
24	1520.0
25	1838.0
26	2157.5
27	2530.5
28	3327.5
29	4509.0
30	5732.0
31	7145.0
32	8542.5
33	9584.0
34	10266.0
35	11513.0
36	13065.0
37	14377.5
38	15751.5
39	16516.0
40	17324.0
41	18765.0
42	18758.5
43	17724.0
44	18262.0
45	19108.0
46	22456.0
47	42339.5
48	81000.5
49	79227.5
50	43501.0
51	27824.5
52	23906.5
53	23449.0
54	22580.0
55	22556.5
56	22627.0
57	21078.5
58	19506.5
59	18749.5
60	17640.5
61	15533.0
62	13888.5
63	11685.0
64	8968.5
65	7519.0
66	6649.5
67	5596.0
68	3917.0
69	2726.0
70	2156.0
71	1665.5
72	1404.5
73	1216.5
74	1125.0
75	1071.0
76	828.5
77	605.0
78	475.5
79	321.0
80	204.0
81	127.5
82	99.5
83	62.0
84	29.0
85	19.5
86	12.0
87	10.5
88	9.0
89	3.0
90	0.0
91	1.0
92	1.5
93	0.5
94	1.0
95	1.0
96	1.5
97	2.0
98	0.5
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33849471806700565
2	0.11766949625220856
3	0.06321286903660948
4	0.03910321689930682
5	0.04270167244218781
6	0.008996138857202489
7	0.006717117013377858
8	0.014393822171523981
9	0.017152638087732746
10-11	0.020511196594421674
12-13	0.014033976617235884
14-15	0.017392535123924812
16-17	0.01913178863631729
18-19	0.01919176289536531
20-21	0.022910166956342338
22-23	0.01565328161153233
24-25	0.024049677878254655
26-27	0.018711968822981175
28-29	0.01757245790106886
30-31	0.018472071786789113
32-33	0.02321003825158242
34-35	0.013194336990563651
36-37	0.008396396266722322
38-39	0.01247464588198745
40-41	0.007976576453386206
42-43	0.007436808121954058
44-45	0.00809652497148224
46-47	0.006837065531473892
48-49	0.0038983268381210783
50-51	0.005817503127657609
52-53	0.006957014049569925
54-55	0.003418532765736946
56-57	0.002518918880016697
58-59	0.0024589446209686804
60-61	0.0031786357295448793
62-63	0.00281879017525678
64-65	0.002039124807632564
66-67	0.0017392535123924812
68-69	0.0035984555428809953
70-71	0.00263886739811273
72-73	0.002518918880016697
74-75	0.00263886739811273
76-77	0.004018275356217111
78-79	0.003718404060977029
80-81	0.003958301097169096
82-83	0.00281879017525678
84-85	0.003238609988592896
86-87	0.0029987129524008294
88-89	0.6131168502478735
90-91	0.6251716763165249
92-93	0.6225328089184122
94-95	0.6086787550783204
96-97	0.6069994758249759
98-99	0.6043006341678152
100	0.6051402737944874
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	833691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.726038211509632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.330687200432	21.352261608073793
2	8.906746083480362	5.1171105666854135
3	4.037175897852854	3.4791620732492037
4	2.4524135247325116	2.817924984875566
5	1.676935221365879	2.40858526235913
6	1.2507924684278373	2.155818734563591
7	0.946880983713517	1.9040097523934423
8	0.7374921737586962	1.6948182691265288
9	0.6217124087098433	1.6073400968251785
>10	4.541259023553692	24.929910496644293
>50	0.34367181108876055	6.788550920024377
>100	0.1387260602027952	6.727149841105942
>500	0.004191119643588979	0.8161527090854246
>1k	0.007124903394101263	5.778683836949729
>5k	0.002933783750512285	5.747020145772643
>10k+	0.0012573358930766938	6.6755007022657535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	28283	3.392503937310107	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	15723	1.8859505500239298	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	11441	1.3723309955367156	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	8967	1.0755783617671295	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	7257	0.8704663958229127	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	7088	0.8501950962646831	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	6911	0.8289642085616854	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	6075	0.7286872474334015	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	5890	0.7064967715856354	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5547	0.6653544298786961	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	4989	0.5984231567811096	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	4835	0.5799510849943205	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	4475	0.5367696184797485	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	4022	0.48243293978224544	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	3901	0.4679191690926255	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	3310	0.3970295948978698	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	3078	0.36920153869959016	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	3004	0.3603253483604837	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	2873	0.3446120924899033	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	2758	0.33081801290885954	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	2263	0.2714434964513231	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	2039	0.24457502839781167	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	1908	0.22886177252723133	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	1214	0.14561750096858428	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	1178	0.1412993543171271	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	1086	0.13026409065229205	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	1065	0.12774517177227535	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	950	0.11395109219123152	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	929	0.11143217331121481	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	2.99871295240083E-4	0.0
16-17	0.0	0.0	0.0	0.0010195624038162822	0.0
18-19	0.0	0.0	0.0	0.001919176289536531	0.0
20-21	0.0	0.0	0.0	0.003118661470496863	0.0
22-23	0.0	0.0	0.0	0.005517631832417526	0.0
24-25	0.0	0.0	0.0	0.009176061634346538	0.0
26-27	0.0	0.0	0.0	0.01583320438867638	0.0
28-29	0.0	0.0	0.0	0.03472509598880161	0.0
30-31	0.0	0.0	0.0	0.07244890493000404	0.0
32-33	0.0	0.0	0.0	0.14363835041999973	0.0
34-35	0.0	0.0	0.0	0.2368383489806175	0.0
36-37	0.0	0.0	0.0	0.36758223370529364	0.0
38-39	0.0	0.0	0.0	0.5695155639199656	0.0
40-41	0.0	0.0	0.0	0.8152900774987375	0.0
42-43	0.0	0.0	0.0	1.0245402673172674	0.0
44-45	0.0	0.0	0.0	1.2486040991206573	0.0
46-47	0.0	0.0	0.0	1.5191479816862603	0.0
48-49	0.0	0.0	0.0	1.7936501653490322	0.0
50-51	0.0	0.0	0.0	2.1092946907187438	0.0
52-53	0.0	0.0	0.0	2.399750027288288	0.0
54-55	0.0	0.0	0.0	2.6853474488749427	0.0
56-57	0.0	0.0	0.0	3.0596467995936143	0.0
58-59	0.0	0.0	0.0	3.4133749794588164	0.0
60-61	0.0	0.0	0.0	3.8175415111834	0.0
62-63	0.0	0.0	0.0	4.205934812778356	0.0
64-65	0.0	0.0	0.0	4.552646004334939	0.0
66-67	0.0	0.0	0.0	4.957352304390955	0.0
68-69	0.0	0.0	0.0	5.458377264478086	0.0
70-71	0.0	0.0	0.0	5.997006084988323	0.0
72-73	0.0	0.0	0.0	6.522680465544188	0.0
74-75	0.0	0.0	0.0	7.055671705703912	0.0
76-77	0.0	0.0	0.0	7.538164619745205	0.0
78-79	0.0	0.0	0.0	8.229188032496452	0.0
80-81	0.0	0.0	0.0	8.954216850127924	0.0
82-83	0.0	0.0	0.0	9.617951975012325	0.0
84-85	0.0	0.0	0.0	10.285585426734846	0.0
86-87	0.0	0.0	0.0	10.92796971539815	0.0
88	0.0	1.1994851809603318E-4	0.0	11.468757609234117	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACACG	45	0.0010172215	41.74914	5
CCATACG	80	2.467259E-7	41.104206	3
TGTATCC	55	0.0027233257	34.164536	3
TTATAGG	55	0.0027233257	34.164536	3
TTGCGTT	200	0.0	32.87745	4
TAGGGTT	60	0.0041743163	31.311853	4
CATACCC	60	0.0041743163	31.311853	4
GACGCTT	90	2.6073905E-5	31.3081	9
CACTACG	60	1.6665217E-7	31.304342	56-57
CTTTGCG	215	0.0	30.602032	2
GAGAACA	790	0.0	29.814093	1
GGTAAGG	65	0.006155781	28.920603	2
CTACGTA	65	3.3620017E-7	28.894585	58-59
TGGGACG	135	4.664871E-7	27.82942	6
GTACATG	7770	0.0	27.645372	1
TTTGCGT	240	0.0	27.402803	3
ATGGGTA	360	0.0	27.397875	5
TACGATG	35	0.008624256	26.991766	82-83
TAGCCGG	70	0.008864243	26.838734	5
GTACAGG	460	0.0	26.625278	1
>>END_MODULE