##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033142_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 833691 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65400730006681 34.0 31.0 34.0 31.0 34.0 2 32.666769822392226 34.0 31.0 34.0 31.0 34.0 3 32.763568276495725 34.0 31.0 34.0 31.0 34.0 4 36.22915924485211 37.0 35.0 37.0 35.0 37.0 5 36.2197732733111 37.0 37.0 37.0 35.0 37.0 6 36.342118362798686 37.0 37.0 37.0 35.0 37.0 7 36.3204268727862 37.0 37.0 37.0 35.0 37.0 8 36.31303684458631 37.0 37.0 37.0 35.0 37.0 9 38.10573461870165 39.0 39.0 39.0 37.0 39.0 10-11 38.075533980815436 39.0 39.0 39.0 35.0 39.0 12-13 38.055251286147985 39.0 39.0 39.0 35.0 39.0 14-15 39.37879022323619 41.0 39.0 41.0 36.0 41.0 16-17 39.46943711758913 41.0 39.0 41.0 36.5 41.0 18-19 39.57992169760739 41.0 40.0 41.0 37.0 41.0 20-21 39.56918870420816 41.0 40.0 41.0 37.0 41.0 22-23 39.44232215533093 41.0 39.0 41.0 36.0 41.0 24-25 39.315810054324686 41.0 39.0 41.0 36.0 41.0 26-27 39.14476346751974 41.0 39.0 41.0 35.0 41.0 28-29 39.048702097059945 41.0 39.0 41.0 35.0 41.0 30-31 38.85462059683984 40.0 38.0 41.0 35.0 41.0 32-33 38.72080543030931 41.0 38.0 41.0 35.0 41.0 34-35 38.50597043748823 41.0 38.0 41.0 35.0 41.0 36-37 38.28437814490021 40.5 37.5 41.0 34.5 41.0 38-39 38.11949331346986 40.0 38.0 41.0 35.0 41.0 40-41 37.99226631929576 40.0 37.5 41.0 34.0 41.0 42-43 37.74116429228575 40.0 37.0 41.0 33.5 41.0 44-45 37.39140460914176 40.0 37.0 41.0 33.0 41.0 46-47 36.811143457228155 40.0 36.5 41.0 31.5 41.0 48-49 36.27603092752591 40.0 36.0 41.0 30.0 41.0 50-51 35.9839790761805 40.0 35.0 41.0 29.0 41.0 52-53 35.88596194513315 40.0 35.0 41.0 29.0 41.0 54-55 35.68983652216468 39.5 35.0 41.0 28.0 41.0 56-57 35.41018374913487 39.0 35.0 41.0 28.0 41.0 58-59 35.15255052531454 39.0 35.0 41.0 27.0 41.0 60-61 34.74039842099771 38.0 35.0 41.0 26.0 41.0 62-63 34.58712520586164 37.0 35.0 40.0 26.0 41.0 64-65 34.28178665716675 37.0 35.0 39.5 26.0 41.0 66-67 33.867954074111395 36.5 35.0 39.0 26.0 41.0 68-69 33.39837481752832 36.0 34.5 39.0 24.5 41.0 70-71 32.875865878365005 35.0 34.0 38.0 23.0 40.0 72-73 32.51830294437627 35.0 34.0 37.0 22.0 39.5 74-75 32.26533931636541 35.0 34.0 37.0 22.0 39.0 76-77 31.978883063389194 35.0 34.0 36.0 20.5 39.0 78-79 31.705434027715306 35.0 33.5 36.0 20.0 37.0 80-81 31.404676912669082 35.0 33.0 35.5 19.0 37.0 82-83 31.102029408977664 35.0 33.0 35.0 16.5 36.5 84-85 30.96576369422244 35.0 33.0 35.0 16.5 36.0 86-87 30.93610282466765 35.0 33.0 35.0 13.5 36.0 88-89 30.772247151522564 35.0 33.0 35.0 8.5 36.0 90-91 30.58087948652438 35.0 33.0 35.0 4.5 35.0 92-93 30.413389373280985 35.0 33.0 35.0 2.0 35.0 94-95 30.2408020477611 35.0 32.5 35.0 2.0 35.0 96-97 30.159262844387186 35.0 32.5 35.0 2.0 35.0 98-99 30.11192636120577 35.0 33.0 35.0 2.0 35.0 100 30.005160185248492 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13922800700878213 1101 2 0.163791956390547 1101 3 0.14394397082414656 1101 4 0.0777107543598845 1101 5 0.05306821501052639 1101 6 0.09922120873953588 1101 7 0.14154694734241247 1101 8 0.19318050728784897 1101 9 0.3110553219238952 1101 10-11 0.23363323242133305 1101 12-13 0.23960157633730716 1101 14-15 0.23790301337940178 1101 16-17 0.20635080059676625 1101 18-19 0.33837371767445745 1101 20-21 0.3491202066060808 1101 22-23 0.33992252873876794 1101 24-25 0.2897849092508338 1101 26-27 0.41722698008272374 1101 28-29 0.3265655834373504 1101 30-31 0.3673326894183617 1101 32-33 0.2382760096472154 1101 34-35 0.19235061807513887 1101 36-37 0.15249111222681933 1101 38-39 0.14504166144899244 1101 40-41 0.20786096749718297 1101 42-43 0.20765654130858024 1101 44-45 0.20372668796972704 1101 46-47 0.39648168188067956 1101 48-49 0.25630879762582026 1101 50-51 0.16140052716415454 1101 52-53 0.24343716836729357 1101 54-55 0.25436827891088853 1101 56-57 0.26741674298033047 1101 58-59 0.2567356214048431 1101 60-61 0.35889122701597387 1101 62-63 0.2801231880777806 1101 64-65 0.171868090872195 1101 66-67 0.11844619135398915 1101 68-69 0.26028555993919866 1101 70-71 0.24313112189141606 1101 72-73 0.27295268274417595 1101 74-75 0.18394926381120769 1101 76-77 0.2247819449550974 1101 78-79 0.15983286997028756 1101 80-81 0.15912865873827187 1101 82-83 0.19377232614322182 1101 84-85 0.1994460885958489 1101 86-87 0.24174820043756284 1101 88-89 0.2871631845334619 1101 90-91 0.2444243072604415 1101 92-93 0.16833338603016657 1101 94-95 0.21369491319695655 1101 96-97 0.27979923405568385 1101 98-99 0.270330015789483 1101 100 0.26848578450866256 1102 1 0.2047648231338357 1102 2 0.2658446096196698 1102 3 0.23092875075010255 1102 4 0.12560873872845235 1102 5 0.14350424956420227 1102 6 0.127653952466396 1102 7 0.19645027930456394 1102 8 0.1910044480529649 1102 9 0.18943458971492788 1102 10-11 0.18832677417370292 1102 12-13 0.21060116498394876 1102 14-15 0.27357146257726583 1102 16-17 0.28231131067492754 1102 18-19 0.23361230261892274 1102 20-21 0.24430531561472435 1102 22-23 0.2840607434652185 1102 24-25 0.34038342837875746 1102 26-27 0.38129115120572266 1102 28-29 0.41290042506631863 1102 30-31 0.525251241454562 1102 32-33 0.4949461042584886 1102 34-35 0.5678277879640774 1102 36-37 0.6086038230454207 1102 38-39 0.728110357458867 1102 40-41 0.7520382607921192 1102 42-43 0.7279280345212555 1102 44-45 0.8388110154069963 1102 46-47 1.0170431792068797 1102 48-49 1.0874215084444074 1102 50-51 1.233514883396154 1102 52-53 1.240934083217148 1102 54-55 1.112379385451412 1102 56-57 1.314683299952776 1102 58-59 1.1305447534492714 1102 60-61 1.2317871546177912 1102 62-63 1.0638109708832175 1102 64-65 1.1468187284698956 1102 66-67 1.2216979396057326 1102 68-69 1.1962090482156285 1102 70-71 1.2148094435697416 1102 72-73 1.262245154977819 1102 74-75 1.2824971864767747 1102 76-77 1.1904442238526727 1102 78-79 1.2194420842853866 1102 80-81 1.2591965320414396 1102 82-83 1.264418150906696 1102 84-85 1.247304994395936 1102 86-87 1.2464561395572673 1102 88-89 1.2486440566025685 1102 90-91 1.195734467688247 1102 92-93 1.334976742673522 1102 94-95 1.2874608531393648 1102 96-97 1.3234870168611117 1102 98-99 1.3909861243867674 1102 100 1.2845861547266821 1103 1 0.16028929865831287 1103 2 0.16797747675254016 1103 3 0.24662805144940592 1103 4 0.11253181565152914 1103 5 0.035112641172595715 1103 6 0.057479127291571785 1103 7 0.1497020275563159 1103 8 0.15180864385716575 1103 9 0.22600801628835399 1103 10-11 0.18951558536251412 1103 12-13 0.10456969645248648 1103 14-15 0.14198055348635563 1103 16-17 0.2140071148707321 1103 18-19 0.20057034457697398 1103 20-21 0.24318643449584698 1103 22-23 0.2178894147938948 1103 24-25 0.2144918199871526 1103 26-27 0.20335408826866086 1103 28-29 0.1710997257656146 1103 30-31 0.3302337589370836 1103 32-33 0.2524985518109375 1103 34-35 0.3024956201319071 1103 36-37 0.30428564122723145 1103 38-39 0.3585649029134075 1103 40-41 0.4547655335193923 1103 42-43 0.3034350275282449 1103 44-45 0.24339143498742288 1103 46-47 0.1580921302558309 1103 48-49 0.2853760538989576 1103 50-51 0.3161722260534958 1103 52-53 0.4004970202800848 1103 54-55 0.3164003644723863 1103 56-57 0.3384420412115148 1103 58-59 0.41269510309962243 1103 60-61 0.46610533643596597 1103 62-63 0.45972006179231073 1103 64-65 0.36804250469366906 1103 66-67 0.4854916458994367 1103 68-69 0.4343034538100383 1103 70-71 0.3575541988144977 1103 72-73 0.39451788225055395 1103 74-75 0.4442279557075466 1103 76-77 0.23257709098554358 1103 78-79 0.3174840423273402 1103 80-81 0.5415741544190595 1103 82-83 0.40544961943816205 1103 84-85 0.38788191747285694 1103 86-87 0.44306453116566047 1103 88-89 0.36373146918998245 1103 90-91 0.3522554467092256 1103 92-93 0.3468298895266706 1103 94-95 0.4496112027897148 1103 96-97 0.41883666721076196 1103 98-99 0.4059686418692827 1103 100 0.36958615472667944 1104 1 0.06690548652449735 1104 2 0.13484338111844352 1104 3 0.1507045492759005 1104 4 0.09403932840904616 1104 5 0.013052775362730529 1104 6 0.07138245369489482 1104 7 0.09778013188442003 1104 8 0.09336624166476071 1104 9 0.17420424573458604 1104 10-11 0.1569662090631354 1104 12-13 0.11014815515594023 1104 14-15 0.25807391957971504 1104 16-17 0.22223452934814958 1104 18-19 0.1185708407024606 1104 20-21 0.14446041389482644 1104 22-23 0.1650727213522032 1104 24-25 0.2337019173222501 1104 26-27 0.24625122491579532 1104 28-29 0.28467407371496023 1104 30-31 0.21435597118428973 1104 32-33 0.06139573570811763 1104 34-35 0.06760972899601825 1104 36-37 0.13421500240659867 1104 38-39 0.21114475549326528 1104 40-41 0.13201522639408125 1104 42-43 0.3716611426293639 1104 44-45 0.3896510031219904 1104 46-47 0.44913470254840604 1104 48-49 0.3660732283461243 1104 50-51 0.35857323720451006 1104 52-53 0.18068684734490859 1104 54-55 0.26574240756443146 1104 56-57 0.19039583066530952 1104 58-59 0.18316913919865385 1104 60-61 0.3856385059691405 1104 62-63 0.34727380872105584 1104 64-65 0.36178187343303136 1104 66-67 0.2559331975909984 1104 68-69 0.29656070669228285 1104 70-71 0.37119764995794924 1104 72-73 0.4704162237738956 1104 74-75 0.4728319530615437 1104 76-77 0.3794184253268753 1104 78-79 0.3256199098382133 1104 80-81 0.33389247798738 1104 82-83 0.42390371601726073 1104 84-85 0.478932758523694 1104 86-87 0.5537211886973168 1104 88-89 0.4341753833838986 1104 90-91 0.42924644557522385 1104 92-93 0.4100888434106231 1104 94-95 0.3701543420828557 1104 96-97 0.42389242226651547 1104 98-99 0.4695672054678468 1104 100 0.44245163384216113 1105 1 0.13326955875916013 1105 2 0.1366545134156283 1105 3 0.0604507136108694 1105 4 -6.16930598120291E-4 1105 5 0.013088111149706094 1105 6 0.06649502935505325 1105 7 0.01556206989297948 1105 8 0.027943071365669425 1105 9 0.0013325686842762252 1105 10-11 0.04953640949326399 1105 12-13 0.09934752936608504 1105 14-15 0.11299349255712343 1105 16-17 0.13654801736446132 1105 18-19 0.07784565335350635 1105 20-21 0.1499876931223696 1105 22-23 0.19194350877210553 1105 24-25 0.05638797756214586 1105 26-27 0.04583287691233551 1105 28-29 0.20197343573079252 1105 30-31 0.14658019961699154 1105 32-33 0.12811524438824762 1105 34-35 0.11370630239468937 1105 36-37 -0.057686728076966176 1105 38-39 0.022922796865238126 1105 40-41 0.049561938918749604 1105 42-43 0.08180826421772736 1105 44-45 0.20166357711928384 1105 46-47 0.3472754858665894 1105 48-49 0.3829644273794486 1105 50-51 0.27721016268618826 1105 52-53 0.3512939161651616 1105 54-55 0.3513998194882504 1105 56-57 0.36245755604942076 1105 58-59 0.36712689976890545 1105 60-61 0.2812621341091699 1105 62-63 0.3848076895050383 1105 64-65 0.35828155194666067 1105 66-67 0.3216833971342581 1105 68-69 0.21995653124941583 1105 70-71 0.4853031418320981 1105 72-73 0.3810288077483506 1105 74-75 0.2550879837025164 1105 76-77 0.27645548494596994 1105 78-79 0.17402557298700216 1105 80-81 0.23205543277974883 1105 82-83 0.25694108326555565 1105 84-85 0.17024070921433676 1105 86-87 0.21231824709698088 1105 88-89 0.16055098478513585 1105 90-91 0.2511849114200686 1105 92-93 0.20449795976651686 1105 94-95 0.31111101347943304 1105 96-97 0.2955583868365004 1105 98-99 0.3510066330003845 1105 100 0.36446832341934865 1106 1 0.005928459497475558 1106 2 0.041008245983306324 1106 3 -7.076128862593123E-4 1106 4 0.021754193273906708 1106 5 -0.005259386799430388 1106 6 0.052017588830032935 1106 7 0.018813915668204118 1106 8 0.053513538962057794 1106 9 0.05481640789674458 1106 10-11 0.12025404690097474 1106 12-13 0.07209207407485962 1106 14-15 0.08804418984998819 1106 16-17 0.06263858340219741 1106 18-19 0.13788502989164897 1106 20-21 0.17039622470563387 1106 22-23 0.19111528891976803 1106 24-25 0.15531070110603196 1106 26-27 0.09510933302390612 1106 28-29 0.106100425066316 1106 30-31 0.260451241454561 1106 32-33 0.34605519516757255 1106 34-35 0.35297324250953466 1106 36-37 0.3576947321363235 1106 38-39 0.1811285392770472 1106 40-41 0.041256442610297483 1106 42-43 0.10432803452125228 1106 44-45 0.057574651770636365 1106 46-47 0.1921522701159688 1106 48-49 0.21631241753532038 1106 50-51 0.10464215612343253 1106 52-53 0.12371590139896682 1106 54-55 0.23552483999687013 1106 56-57 0.31277420904368114 1106 58-59 0.31672657163109363 1106 60-61 0.2125871546177862 1106 62-63 0.19481097088321775 1106 64-65 0.2323278193789804 1106 66-67 0.35346157596936933 1106 68-69 0.3851181391247209 1106 70-71 0.3327003526606518 1106 72-73 0.3942087913414625 1106 74-75 0.3518062773858617 1106 76-77 0.21868058748903252 1106 78-79 0.2926057206490249 1106 80-81 0.2685965320414354 1106 82-83 0.24569087817942048 1106 84-85 0.1888868125777492 1106 86-87 0.20400159410272067 1106 88-89 0.27937132932984454 1106 90-91 0.3053890131427899 1106 92-93 0.24599492449170413 1106 94-95 0.0859881258666384 1106 96-97 0.18008701686111195 1106 98-99 0.21038612438676552 1106 100 0.22640433654486358 1107 1 0.003088024038689241 1107 2 0.04237931907817227 1107 3 0.04788336689600925 1107 4 0.07996756807951044 1107 5 -0.01890915351793865 1107 6 -0.03094353092113522 1107 7 -0.017590438919668827 1107 8 0.03194464316112544 1107 9 -0.04295901978598948 1107 10-11 0.0449109675852668 1107 12-13 0.04838569771405332 1107 14-15 0.06440562064314292 1107 16-17 0.09029519304450417 1107 18-19 0.082619088989631 1107 20-21 0.07791410962009593 1107 22-23 -0.05096682616576942 1107 24-25 0.051578819301987266 1107 26-27 0.08905023504567566 1107 28-29 -0.019665671356698056 1107 30-31 0.12175201905954225 1107 32-33 0.16199049843351787 1107 34-35 0.22532036537111821 1107 36-37 0.17970095297614108 1107 38-39 0.019617497286382957 1107 40-41 -0.06674231322173796 1107 42-43 -0.0568931163341162 1107 44-45 -0.02680046487011367 1107 46-47 -0.16375068478293997 1107 48-49 0.039272915202502645 1107 50-51 0.05883935674550855 1107 52-53 0.08400703670223209 1107 54-55 0.10102281822392456 1107 56-57 -0.0136519184835322 1107 58-59 0.030623927774172444 1107 60-61 0.06587735679508455 1107 62-63 0.025498373682594888 1107 64-65 -0.06105134080763719 1107 66-67 0.08867588390094028 1107 68-69 0.046620471939647246 1107 70-71 0.03696411626874152 1107 72-73 0.009479708915336005 1107 74-75 0.0012289834511847175 1107 76-77 0.041288985622784935 1107 78-79 0.09067352780298776 1107 80-81 0.14761799393879116 1107 82-83 0.16195526387149073 1107 84-85 0.12639661040045524 1107 86-87 0.2478367418476637 1107 88-89 0.28812094052735304 1107 90-91 0.2729852806389026 1107 92-93 0.17553520442949733 1107 94-95 0.09887397345606175 1107 96-97 0.12774051608350945 1107 98-99 0.15880105440231773 1107 100 0.13713528522293572 1108 1 0.13608314227693086 1108 2 0.0742928381283221 1108 3 0.05170326324667229 1108 4 0.10269195762436567 1108 5 0.01846991833652112 1108 6 0.06999946194181206 1108 7 0.10541210297937909 1108 8 0.0012186749137228503 1108 9 0.08816021454327228 1108 10-11 0.03700570853239782 1108 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-0.13488744586746293 2214 78-79 -0.3771963641320788 2214 80-81 -0.271647804303246 2214 82-83 -0.3866690940234996 2214 84-85 -0.3391031804729785 2214 86-87 -0.5044223113872022 2214 88-89 -0.6801853072233222 2214 90-91 -0.41795970124220005 2214 92-93 -0.3952203639030145 2214 94-95 -0.5410106232647038 2214 96-97 -0.5689000574960801 2214 98-99 -0.5468422286361658 2214 100 -0.6413510491396401 2215 1 -0.028003716737508455 2215 2 -0.07697733479106006 2215 3 -0.049716958680654955 2215 4 -0.09095395091834746 2215 5 -0.031243899483008875 2215 6 -0.10030817886022447 2215 7 -0.13958928860416364 2215 8 -0.15452170803393273 2215 9 -0.13238359210325257 2215 10-11 -0.12130910396684413 2215 12-13 -0.18377922098522248 2215 14-15 -0.2389288408576249 2215 16-17 -0.15916702407444205 2215 18-19 -0.24295342137670417 2215 20-21 -0.24147026394590654 2215 22-23 -0.22315493804952524 2215 24-25 -0.1980308209233428 2215 26-27 -0.20859961223644774 2215 28-29 -0.2196362905545115 2215 30-31 -0.2565912151542449 2215 32-33 -0.2775426686508453 2215 34-35 -0.14246654387230961 2215 36-37 -0.09604358562068427 2215 38-39 -0.15610617367355673 2215 40-41 -0.11695130104791218 2215 42-43 -0.17396782662694932 2215 44-45 -0.24192815196767725 2215 46-47 -0.28866321720045107 2215 48-49 -0.10891481877976616 2215 50-51 -0.17999282384987225 2215 52-53 -0.21596420541011696 2215 54-55 -0.23811521340766717 2215 56-57 -0.32174648521532845 2215 58-59 -0.40089959659320584 2215 60-61 -0.2805492939803429 2215 62-63 -0.2837257447376089 2215 64-65 -0.3146019536517244 2215 66-67 -0.4873923626154095 2215 68-69 -0.5663798582050532 2215 70-71 -0.47650018138474337 2215 72-73 -0.2950227173367779 2215 74-75 -0.2618807720133347 2215 76-77 -0.374056929199881 2215 78-79 -0.4621382045846225 2215 80-81 -0.5258969259024902 2215 82-83 -0.3689366251583621 2215 84-85 -0.37764402854374524 2215 86-87 -0.49366462752611895 2215 88-89 -0.4133950258103454 2215 90-91 -0.42985905094265675 2215 92-93 -0.4564894546805256 2215 94-95 -0.5437838367501868 2215 96-97 -0.44947025950737896 2215 98-99 -0.622945784825518 2215 100 -0.7311390546433891 2216 1 -0.22094727682791415 2216 2 -0.23968516215133917 2216 3 -0.2779811051723726 2216 4 -0.1399299581987421 2216 5 -0.13837130825805843 2216 6 -0.1897832526846983 2216 7 -0.2123613998998195 2216 8 -0.06932068263682112 2216 9 -0.21513254020984363 2216 10-11 -0.2556780709110882 2216 12-13 -0.14342153041322092 2216 14-15 -0.39513056470821084 2216 16-17 -0.3405154137927511 2216 18-19 -0.3392201594491624 2216 20-21 -0.4124312647754351 2216 22-23 -0.3677497882190863 2216 24-25 -0.5172048669164084 2216 26-27 -0.6618135281261672 2216 28-29 -0.5842805005998812 2216 30-31 -0.4834444107193505 2216 32-33 -0.5142079184369166 2216 34-35 -0.5424304040542793 2216 36-37 -0.6422351416364691 2216 38-39 -0.8018807173849396 2216 40-41 -0.7029387888062502 2216 42-43 -0.5750032417760877 2216 44-45 -0.5137033285940262 2216 46-47 -0.5893198196455955 2216 48-49 -0.4911523790986223 2216 50-51 -0.5626638747321095 2216 52-53 -0.4437522612938807 2216 54-55 -0.4878788065669539 2216 56-57 -0.35373630989039384 2216 58-59 -0.5056072292104261 2216 60-61 -0.5148888622125085 2216 62-63 -0.362605578906404 2216 64-65 -0.3996704975915648 2216 66-67 -0.49581640439529195 2216 68-69 -0.47829560561581275 2216 70-71 -0.46782475252869204 2216 72-73 -0.41641224652670417 2216 74-75 -0.5578139189675682 2216 76-77 -0.4497412918938508 2216 78-79 -0.6497062008096073 2216 80-81 -0.7804878999361229 2216 82-83 -0.8071450264489108 2216 84-85 -0.9327389938738619 2216 86-87 -1.042385502671472 2216 88-89 -0.9950101573461758 2216 90-91 -0.9902345492695481 2216 92-93 -1.1264449072053502 2216 94-95 -1.1913897142455667 2216 96-97 -1.4829155076830673 2216 98-99 -1.3886001308727032 2216 100 -1.412305340874493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 7.0 4 20.0 5 42.0 6 97.0 7 225.0 8 340.0 9 487.0 10 791.0 11 1489.0 12 2462.0 13 3897.0 14 3976.0 15 4989.0 16 6646.0 17 11299.0 18 13553.0 19 6487.0 20 3375.0 21 2849.0 22 2900.0 23 3028.0 24 3225.0 25 3912.0 26 4739.0 27 5655.0 28 6030.0 29 7468.0 30 9284.0 31 11768.0 32 14730.0 33 19558.0 34 28021.0 35 48607.0 36 98658.0 37 210707.0 38 233574.0 39 58716.0 40 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.25548304063236 17.2944490066416 13.163859714838665 19.28620823788737 2 16.490220491746253 23.489292080193646 35.00111553326089 25.019371894799214 3 24.601081215942116 25.556351214058925 29.97885307625967 19.863714493739288 4 12.37502803810063 16.21867679356881 38.660312563348114 32.745982604982444 5 11.683045945978376 40.41830159472983 32.873273575962656 15.025378883329132 6 28.842100970263562 36.48474074927041 16.050671051984487 18.622487228481535 7 25.998692520376892 34.490592744087856 21.09528407693738 18.415430658597877 8 26.930698294805733 27.29930141731679 19.93251672088359 25.837483566993885 9 25.450196775543937 17.32692328452628 22.474274041581353 34.74860589834843 10-11 26.987242806498607 23.614212296828605 25.3218086175079 24.07673627916489 12-13 31.004712777275994 22.960065539870286 25.361135000857633 20.674086681996087 14-15 25.101761385070358 24.03583823973045 29.421529932271028 21.440870442928162 16-17 20.985233137937197 27.28768812425707 28.54408887705397 23.182989860751764 18-19 20.452566301622905 27.76673583706174 30.318103851551538 21.462594009763823 20-21 24.1224266544799 22.335973400216627 29.37341293116994 24.168187014133533 22-23 22.204862964486757 23.52675877935297 30.790498615191037 23.477879640969235 24-25 25.08933165885202 23.465468620867924 29.869280104978944 21.57591961530111 26-27 23.2616650183494 21.28200499353168 34.473301158660085 20.98302882945884 28-29 20.642523742860945 27.077417861157194 31.21659894987998 21.063459446101877 30-31 22.16496051441945 23.085760773117048 30.695021811111157 24.054256901352343 32-33 22.330176296548697 23.292464624311023 31.75623468773171 22.62112439140857 34-35 24.489434750292006 22.417358408616575 31.064347748881627 22.028859092209796 36-37 23.004991121562604 21.931240101742095 33.54009694293804 21.52367183375726 38-39 22.363208373425604 24.169940035561144 31.888951810091015 21.577899780922237 40-41 22.116337554322136 25.242184931621903 30.564374278604454 22.077103235451506 42-43 21.822019918201242 24.84760212547195 28.230648979195184 25.09972897713162 44-45 23.110788245837778 22.247210288071276 29.321339061725787 25.32066240436516 46-47 25.34999127233727 24.398766010462396 29.13455776543042 21.116684951769916 48-49 24.25473848368522 24.056741842610364 28.723368522072935 22.965151151631478 50-51 20.247498415048078 25.222897921898607 31.537514327545534 22.99208933550778 52-53 22.994439436278434 26.404850543730884 26.540340927152478 24.0603690928382 54-55 21.51635214693901 23.846521090335056 28.387084778780597 26.250041983945337 56-57 24.25658400191443 22.7535285308249 27.612054431707566 25.3778330355531 58-59 26.711715872138143 23.01467827622316 26.82404900675438 23.449556844884313 60-61 23.324503065004727 24.227721364951172 30.361689150165137 22.08608641987896 62-63 21.30970073613465 24.653133880066548 29.6043201280109 24.4328452557879 64-65 21.090700592323543 27.398622469910013 27.925812015843277 23.584864921923167 66-67 22.905986186645812 24.01059871462397 26.448147144128605 26.63526795460161 68-69 21.810461416666417 24.49577626974298 27.553055269841938 26.14070704374867 70-71 24.82321013569233 24.32200314866182 26.58082315016118 24.273963565484667 72-73 23.491356173626208 22.18727041007572 30.41355423944824 23.90781917684984 74-75 21.41485868505885 27.228008096558966 27.35719319289302 23.999940025489167 76-77 22.780164761877344 23.53344416616288 26.87072381994223 26.81566725201754 78-79 23.36360037903777 24.051805827105998 27.70340294354017 24.881190850316067 80-81 24.65689999406251 23.170907974290063 27.340166045829033 24.832025985818394 82-83 23.076526322228847 22.492973981842106 29.886636111634225 24.543863584294826 84-85 22.290939601541822 24.507235016435995 28.476703229083768 24.725122152938418 86-87 22.23771560035241 25.243630872165234 27.351370024451548 25.167283503030806 88-89 21.48949670801292 24.996131660291404 25.283048515577118 28.231323116118563 90-91 23.09368818902927 22.590204284321167 26.669353513472018 27.646754013177542 92-93 24.50914067682151 24.381095633753993 27.015165091142883 24.094598598281618 94-95 22.87646142275735 23.352057297008123 27.72783921141046 26.043642068824063 96-97 20.063728647664423 23.849663724329517 30.342716905903988 25.743890722102076 98-99 22.278038265976242 25.098927540299705 25.93520860846525 26.687825585258807 100 21.750864528944177 21.087789120909306 26.503344764427105 30.658001585719408 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 632.0 1 392.5 2 511.0 3 997.0 4 667.0 5 321.0 6 698.0 7 1447.0 8 2384.5 9 2779.5 10 1889.5 11 913.5 12 804.0 13 820.5 14 853.0 15 974.5 16 1078.5 17 1209.5 18 1335.5 19 1393.5 20 1478.0 21 1594.5 22 1672.0 23 1832.0 24 2145.0 25 2426.0 26 2629.5 27 2972.0 28 3727.5 29 4838.5 30 5969.0 31 7308.5 32 8613.5 33 9637.5 34 10404.5 35 11532.0 36 12829.0 37 13925.0 38 15112.0 39 15804.5 40 16493.0 41 17719.5 42 17717.0 43 16859.0 44 17564.0 45 18434.5 46 20694.0 47 41892.0 48 82373.5 49 77090.5 50 41819.5 51 29396.0 52 25231.5 53 23615.0 54 21867.5 55 22070.0 56 22454.0 57 20919.5 58 19285.0 59 18775.5 60 17635.5 61 15579.5 62 13950.5 63 11552.5 64 8711.0 65 7249.0 66 6446.5 67 5384.5 68 3733.5 69 2609.0 70 2006.0 71 1530.0 72 1328.0 73 1135.5 74 1076.0 75 1016.5 76 704.5 77 487.0 78 374.5 79 266.5 80 171.5 81 94.5 82 77.0 83 39.5 84 8.0 85 6.5 86 3.5 87 3.5 88 3.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09104092523488919 2 0.0010795366628642987 3 0.0 4 4.797940723841327E-4 5 0.038503474308826655 6 0.0 7 0.0031186614704968624 8 3.5984555428809954E-4 9 0.0 10-11 5.397683314321493E-4 12-13 0.0 14-15 5.997425904801659E-5 16-17 0.0 18-19 1.1994851809603318E-4 20-21 0.0 22-23 2.3989703619206636E-4 24-25 0.0 26-27 0.0014993564762004147 28-29 0.00863629330291439 30-31 0.034245301916417475 32-33 0.025069240282070936 34-35 0.02728828786684755 36-37 0.025309137318263 38-39 0.023629858064918536 40-41 0.028427798788759864 42-43 0.02183063029347804 44-45 0.02183063029347804 46-47 0.016133075683916465 48-49 0.01091531514673902 50-51 0.008336422007674307 52-53 0.006297297200041741 54-55 0.004677992205745294 56-57 0.002698841657160747 58-59 0.001199485180960332 60-61 6.597168495281825E-4 62-63 0.0014393822171523982 64-65 0.0029987129524008294 66-67 5.99742590480166E-4 68-69 0.001019562403816282 70-71 4.198198133361161E-4 72-73 4.198198133361161E-4 74-75 4.198198133361161E-4 76-77 3.5984555428809954E-4 78-79 1.1994851809603318E-4 80-81 6.597168495281825E-4 82-83 4.797940723841327E-4 84-85 2.99871295240083E-4 86-87 1.7992277714404977E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 833691.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.558802734964292 #Duplication Level Percentage of deduplicated Percentage of total 1 68.39860587639865 16.113892631886454 2 10.738949291220235 5.059935758652843 3 4.867361994476928 3.4400766320263285 4 3.099295986188616 2.9206281102353686 5 2.0426166895751474 2.406080182642335 6 1.5619195304187408 2.2078172463013903 7 1.1900380918620623 1.962511085729015 8 0.9388397476856022 1.7694352332374998 9 0.8007867820646497 1.6979020048285134 >10 5.695814939700994 25.63572479219945 >50 0.45368051551210303 7.32547025825436 >100 0.18666003272651305 7.589918584249925 >500 0.008137767094343894 1.3007933970701921 >1k 0.01068081931132636 5.992684245363994 >5k 0.005086104433964934 7.884801833162023 >10k+ 0.00152583133018948 6.692328004160314 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 27748 3.3283314801287287 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16214 1.944845272409082 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11890 1.4261878801618344 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9210 1.1047258516644656 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7454 0.8940962538878314 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6907 0.8284844144893012 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6878 0.8250059074645162 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6771 0.8121714160282407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6410 0.7688700009955727 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6033 0.7236494096733682 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5780 0.6933024345950718 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5251 0.6298496685222702 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5110 0.6129369274707296 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4457 0.5346105451540198 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4107 0.4926285638204083 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4048 0.4855516012527423 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3469 0.4161014092751391 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3418 0.40998403485224144 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3262 0.39127206602926023 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3089 0.3705209723986465 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3048 0.36560308315670914 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2804 0.33633564474127703 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2515 0.3016705230115234 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2107 0.25273152762834195 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1975 0.2368983232396655 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1525 0.18292149009645062 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 1512 0.18136215936120217 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1365 0.16372972720108528 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1329 0.1594115805496281 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1270 0.15233461798196216 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1237 0.14837631688479305 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1195 0.14333847912475967 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1157 0.13878043543711038 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1124 0.1348221343399413 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 967 0.1159902169988641 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 958 0.11491068033599978 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.198198133361161E-4 0.0 16-17 0.0 0.0 0.0 0.0011395109219123152 0.0 18-19 0.0 0.0 0.0 0.002039124807632564 0.0 20-21 0.0 0.0 0.0 0.003238609988592896 0.0 22-23 0.0 0.0 0.0 0.00563758035051356 0.0 24-25 0.0 0.0 0.0 0.009176061634346538 0.0 26-27 0.0 0.0 0.0 0.01619304994296448 0.0 28-29 0.0 0.0 0.0 0.03538481283832979 0.0 30-31 0.0 0.0 0.0 0.07310862177953223 0.0 32-33 0.0 0.0 0.0 0.14471788708286404 0.0 34-35 0.0 0.0 0.0 0.23761801434824176 0.0 36-37 0.0 0.0 0.0 0.36914156444054214 0.0 38-39 0.0 0.0 0.0 0.5719145342818862 0.0 40-41 0.0 0.0 0.0 0.8183487647101864 0.0 42-43 0.0 0.0 0.0 1.029818002113493 0.0 44-45 0.0 0.0 0.0 1.2561008815016594 0.0 46-47 0.0 0.0 0.0 1.5279042235072706 0.0 48-49 0.0 0.0 0.0 1.804145660682435 0.0 50-51 0.0 0.0 0.0 2.122549001968355 0.0 52-53 0.0 0.0 0.0 2.415523257417916 0.0 54-55 0.0 0.0 0.0 2.704359288993164 0.0 56-57 0.0 0.0 0.0 3.0820771724775726 0.0 58-59 0.0 0.0 0.0 3.439163910849463 0.0 60-61 0.0 0.0 0.0 3.846689001080736 0.0 62-63 0.0 0.0 0.0 4.238620783959524 0.0 64-65 0.0 0.0 0.0 4.588450636986606 0.0 66-67 0.0 0.0 0.0 4.9983746975797985 0.0 68-69 0.0 0.0 0.0 5.506476620234595 0.0 70-71 0.0 0.0 0.0 6.055900807373476 0.0 72-73 0.0 0.0 0.0 6.588772099015102 0.0 74-75 0.0 0.0 0.0 7.130339658218692 0.0 76-77 0.0 0.0 0.0 7.62026938038194 0.0 78-79 0.0 0.0 0.0 8.32208815976183 0.0 80-81 0.0 0.0 0.0 9.06145082530578 0.0 82-83 0.0 0.0 0.0 9.739519798102656 0.0 84-85 0.0 0.0 0.0 10.420827380888124 0.0 86-87 0.0 0.0 0.0 11.07586623821056 0.0 88 0.0 0.0 0.0 11.627929292747552 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGAGT 80 4.642061E-9 46.994576 6 GTTGGAC 60 8.796852E-5 39.185646 3 GGAGATA 90 6.19606E-7 36.549145 8 GGACATA 65 1.4141169E-4 36.14967 7 AGTTTAC 55 0.0027189204 34.17582 8 GTATATA 140 5.0386006E-10 33.615948 1 TTGCGTT 180 7.2759576E-12 31.348518 4 ATACTTA 170 1.2187229E-10 30.408253 6 TCTATAG 170 4.0527084E-9 27.660456 3 GTACATG 7345 0.0 26.91106 1 TAGGACG 70 0.008813801 26.870161 5 TACTAGA 105 7.3947434E-5 26.870157 2 AGACTCG 35 0.008836947 26.857264 54-55 GGACGTA 70 0.00883963 26.854042 7 GACTGTT 70 0.00883963 26.854042 7 AGATACG 70 6.3809784E-7 26.85243 10-11 CTAGCCG 80 6.931805E-8 26.432861 76-77 TACATGG 7525 0.0 25.995314 2 ACCGTTC 145 8.658935E-7 25.926485 8 CTTTGCG 220 8.731149E-11 25.648787 2 >>END_MODULE