FastQCFastQC Report
Wed 25 May 2016
SRR1033141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4079125
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG125650.027.7794741
GTATCAA61150.027.57751
TACATGG126000.026.4519882
TCAACGC62250.025.7338394
GGTATCA43450.025.3684751
ATCAACG63450.025.3258443
ACATGGG128600.024.9179843
CAACGCA66700.024.0169645
AACGCAG68650.023.3267546
TATCAAC73650.022.2759022
CATGGGG92200.020.2787744
ACGCAGA82000.019.1281557
GAGTACA83800.018.3811231
GTATAGA15000.018.2139871
GTATAGG14300.017.7879541
CGCAGAG86700.017.6578988
AGTACAT87050.016.416762
TCTATAC14150.015.6066943
GTATTAG19950.015.5836551
TATACCG2751.2539569E-515.3744435
Produced by FastQC (version 0.11.5)