##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033141_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4079125
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.388849079153005	33.0	31.0	34.0	30.0	34.0
2	31.43820010418901	33.0	31.0	34.0	28.0	34.0
3	31.62957178316428	34.0	31.0	34.0	30.0	34.0
4	35.03422915453682	37.0	35.0	37.0	33.0	37.0
5	35.09839977936445	37.0	35.0	37.0	33.0	37.0
6	35.17544081144853	37.0	35.0	37.0	33.0	37.0
7	35.07077547268103	37.0	35.0	37.0	33.0	37.0
8	35.0794300248215	37.0	35.0	37.0	33.0	37.0
9	36.72297269635032	39.0	38.0	39.0	33.0	39.0
10-11	36.72505206386174	39.0	37.5	39.0	33.0	39.0
12-13	36.69174075322526	39.0	38.0	39.0	33.0	39.0
14-15	38.095922287255235	41.0	39.0	41.0	33.0	41.0
16-17	38.08042827812337	41.0	38.5	41.0	33.0	41.0
18-19	38.0438350136365	40.5	38.5	41.0	33.0	41.0
20-21	38.065725124873595	40.5	39.0	41.0	33.0	41.0
22-23	38.00918836760334	40.0	38.5	41.0	33.0	41.0
24-25	37.98526693837526	40.0	38.0	41.0	33.0	41.0
26-27	37.9321837403855	40.0	38.0	41.0	33.0	41.0
28-29	37.8971910642601	40.0	38.0	41.0	33.0	41.0
30-31	37.89036239389575	40.0	38.0	41.0	33.0	41.0
32-33	37.96584720987957	40.0	38.0	41.0	33.0	41.0
34-35	37.933390126559004	40.0	38.0	41.0	33.0	41.0
36-37	37.84695222627401	40.0	38.0	41.0	33.0	41.0
38-39	37.76080801642509	40.0	38.0	41.0	32.0	41.0
40-41	37.69155541936077	40.0	38.0	41.0	32.0	41.0
42-43	37.562623111574176	40.0	38.0	41.0	32.0	41.0
44-45	37.413804308522046	40.0	38.0	41.0	31.5	41.0
46-47	37.27708466889345	40.0	37.5	41.0	31.0	41.0
48-49	37.1440306438268	40.0	37.0	41.0	31.0	41.0
50-51	37.00304501578157	40.0	37.0	41.0	31.0	41.0
52-53	36.841879079459446	40.0	36.5	41.0	30.0	41.0
54-55	36.66892005025588	40.0	36.0	41.0	30.0	41.0
56-57	36.49553740079061	40.0	35.5	41.0	30.0	41.0
58-59	36.28066288726136	39.0	35.0	41.0	30.0	41.0
60-61	36.043756320289276	39.0	35.0	41.0	30.0	41.0
62-63	35.76652970919008	39.0	35.0	41.0	29.0	41.0
64-65	35.487009346367174	38.0	35.0	40.0	29.0	41.0
66-67	35.182974657555235	37.5	35.0	40.0	29.0	41.0
68-69	34.86719933809334	37.0	34.0	40.0	28.5	41.0
70-71	34.5147103851929	36.5	34.0	39.5	28.0	41.0
72-73	34.14647773725983	36.0	34.0	39.0	28.0	41.0
74-75	33.77662341801244	35.5	34.0	39.0	27.0	40.5
76-77	33.45227702019429	35.0	34.0	37.5	27.0	40.0
78-79	33.08989918180983	35.0	33.5	37.0	26.5	39.0
80-81	32.73805472987466	35.0	33.0	37.0	26.0	39.0
82-83	32.5349893665921	35.0	33.0	36.0	26.0	38.5
84-85	32.274163454172154	35.0	33.0	36.0	26.0	37.0
86-87	32.03122507890785	35.0	33.0	35.5	26.0	37.0
88-89	31.68271320442497	35.0	33.0	35.0	25.0	36.5
90-91	31.494953942328316	35.0	33.0	35.0	24.5	36.0
92-93	31.31801697668005	35.0	33.0	35.0	24.0	36.0
94-95	31.141445653173168	35.0	33.0	35.0	23.5	36.0
96-97	30.959316642662337	35.0	33.0	35.0	21.5	35.5
98-99	30.756054546011704	35.0	33.0	35.0	19.5	35.0
100	30.57999816137039	35.0	32.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29991424235516817
1101	2	0.308822983856345
1101	3	0.3235293945668012
1101	4	0.26315625914925533
1101	5	0.28464198683062847
1101	6	0.2389896106541869
1101	7	0.28766806698401837
1101	8	0.21819753127749664
1101	9	0.3108569681190261
1101	10-11	0.32788303948017017
1101	12-13	0.37974117046113776
1101	14-15	0.4350439615117807
1101	16-17	0.3611151953283098
1101	18-19	0.42215200839606837
1101	20-21	0.3588731295430705
1101	22-23	0.3719949726061955
1101	24-25	0.3104690804235659
1101	26-27	0.3557250343915399
1101	28-29	0.33717009138523935
1101	30-31	0.33137790794165056
1101	32-33	0.4148760183290676
1101	34-35	0.48818132756747445
1101	36-37	0.43717904477254166
1101	38-39	0.4080846204833293
1101	40-41	0.38193205365103466
1101	42-43	0.3907307622553162
1101	44-45	0.41015397415096544
1101	46-47	0.49604173011979924
1101	48-49	0.4477013909189793
1101	50-51	0.45381710908575457
1101	52-53	0.48517657095233346
1101	54-55	0.3959646992568935
1101	56-57	0.4622684868379707
1101	58-59	0.4608338839691797
1101	60-61	0.4100672199867219
1101	62-63	0.4446690271646361
1101	64-65	0.4275890719501376
1101	66-67	0.3965925759431954
1101	68-69	0.4354104175200533
1101	70-71	0.40560721346241024
1101	72-73	0.43952259110251646
1101	74-75	0.40421749268374896
1101	76-77	0.41647234763793506
1101	78-79	0.3622922127819095
1101	80-81	0.42392932097416747
1101	82-83	0.3907072524987072
1101	84-85	0.3339349126740885
1101	86-87	0.4142067089101005
1101	88-89	0.6089890757565755
1101	90-91	0.6224484317657399
1101	92-93	0.6452605377967231
1101	94-95	0.6684246082946643
1101	96-97	0.6790947875294862
1101	98-99	0.7260301960647695
1101	100	0.7539893137187299
1102	1	0.17543728987294216
1102	2	0.16010336814137816
1102	3	0.17031753743897937
1102	4	0.10808218572321948
1102	5	0.0508365473192498
1102	6	0.07392631873811695
1102	7	0.006087820402512989
1102	8	0.11586220555629012
1102	9	0.20731970707956293
1102	10-11	0.17347393138277312
1102	12-13	0.14927707522659972
1102	14-15	0.13255821760064634
1102	16-17	0.19053514434693142
1102	18-19	0.17705239204912004
1102	20-21	0.2318587127173828
1102	22-23	0.25883124446789907
1102	24-25	0.26003098446668815
1102	26-27	0.3206594365354647
1102	28-29	0.2676799332834179
1102	30-31	0.2610950666422909
1102	32-33	0.2899328833840542
1102	34-35	0.28268232985487884
1102	36-37	0.27875352389256136
1102	38-39	0.22186801776754095
1102	40-41	0.2820743566557269
1102	42-43	0.30187331961850816
1102	44-45	0.28448600561620907
1102	46-47	0.19509210981645708
1102	48-49	0.21291041014758605
1102	50-51	0.21265213344175748
1102	52-53	0.17903943437617897
1102	54-55	0.18888188571759912
1102	56-57	0.13515513069245344
1102	58-59	0.14197603132945602
1102	60-61	0.13854240932580808
1102	62-63	0.23223149711625268
1102	64-65	0.2678019072091402
1102	66-67	0.19896729915857492
1102	68-69	0.14057648371960596
1102	70-71	0.15622685322914975
1102	72-73	0.19424020354843208
1102	74-75	0.1335388190249276
1102	76-77	0.11709596665277644
1102	78-79	0.14959435046402803
1102	80-81	0.13714771725687314
1102	82-83	0.09219819417618424
1102	84-85	0.11895606634396927
1102	86-87	0.1340196561756386
1102	88-89	0.2233458928124925
1102	90-91	0.2496139882036914
1102	92-93	0.2942606105265426
1102	94-95	0.32199407545539316
1102	96-97	0.3600578360814026
1102	98-99	0.3806764595148877
1102	100	0.374148504534876
1103	1	0.138839306732347
1103	2	0.11575418522139458
1103	3	0.15552835935977782
1103	4	0.20119801130435633
1103	5	0.1721013102103015
1103	6	0.16902086274259176
1103	7	0.09465524711131934
1103	8	0.09639244951969772
1103	9	0.0998781981846335
1103	10-11	0.1414741932610326
1103	12-13	0.159113505468774
1103	14-15	0.14704038309581335
1103	16-17	0.17397332818149636
1103	18-19	0.24538191128427655
1103	20-21	0.1360012250822038
1103	22-23	0.17420897906222166
1103	24-25	0.17982295492206646
1103	26-27	0.22555708082145998
1103	28-29	0.188416500614899
1103	30-31	0.11564091120960995
1103	32-33	0.10835217528109808
1103	34-35	0.15316133396358822
1103	36-37	0.15621768426521498
1103	38-39	0.14536236655367674
1103	40-41	0.14787476673327404
1103	42-43	0.10522382383336293
1103	44-45	0.024461102382211664
1103	46-47	0.08304132031301492
1103	48-49	0.042492014985199944
1103	50-51	-0.029791339540146566
1103	52-53	-0.016985124646609506
1103	54-55	0.060915420022659816
1103	56-57	0.02065617622157845
1103	58-59	0.0735385121101686
1103	60-61	0.03997029063147295
1103	62-63	0.08169253875688298
1103	64-65	0.11997807589047937
1103	66-67	0.08837568739706825
1103	68-69	0.03563310993401103
1103	70-71	0.06018709237161346
1103	72-73	0.12343065253077867
1103	74-75	0.11930390033614202
1103	76-77	0.1161890643750354
1103	78-79	0.07636440944614975
1103	80-81	0.09900165168017594
1103	82-83	0.10558659634183698
1103	84-85	0.10011298300518234
1103	86-87	0.11787124063832266
1103	88-89	0.17297660974391604
1103	90-91	0.21197493977406623
1103	92-93	0.17164280994683878
1103	94-95	0.11956428660466045
1103	96-97	0.1369290009040114
1103	98-99	0.18695164297846745
1103	100	0.230954165073193
1104	1	0.19503675032009582
1104	2	0.19109884895055274
1104	3	0.20701441758020067
1104	4	0.17479471890058562
1104	5	0.1389565319809165
1104	6	0.18451008063766494
1104	7	0.16189980289125572
1104	8	0.1779322070184648
1104	9	0.19266005530999308
1104	10-11	0.23682678703301008
1104	12-13	0.23050988133714156
1104	14-15	0.17981091124947568
1104	16-17	0.24690907228583114
1104	18-19	0.233380808531237
1104	20-21	0.22955923425931246
1104	22-23	0.2153901353246468
1104	24-25	0.2623216874444054
1104	26-27	0.25585923183074044
1104	28-29	0.315867669495141
1104	30-31	0.2907740438352491
1104	32-33	0.28680905445678206
1104	34-35	0.2721780798594722
1104	36-37	0.25780857048370365
1104	38-39	0.21516585055070436
1104	40-41	0.28418380057350845
1104	42-43	0.2331261459283951
1104	44-45	0.2197725268656967
1104	46-47	0.19904845948775574
1104	48-49	0.09726289347538852
1104	50-51	0.2032111085431083
1104	52-53	0.10949502342516126
1104	54-55	0.126290802283485
1104	56-57	0.13535109624706365
1104	58-59	0.10666210637300821
1104	60-61	0.08439472997435615
1104	62-63	0.05788146366049318
1104	64-65	-0.004843492211996647
1104	66-67	0.04015682581037794
1104	68-69	-0.008806460813552519
1104	70-71	0.04750252483783868
1104	72-73	0.1332092189613192
1104	74-75	0.07593741320507519
1104	76-77	0.026353202732323666
1104	78-79	-0.006184345719887574
1104	80-81	0.06448976587279986
1104	82-83	0.023216866461737595
1104	84-85	0.0812282185613995
1104	86-87	0.10106161483380305
1104	88-89	0.22033517680107906
1104	90-91	0.1806011292804932
1104	92-93	0.146284889920409
1104	94-95	0.20585488980060163
1104	96-97	0.21415488393718363
1104	98-99	0.2522153495388757
1104	100	0.247775777323767
1105	1	0.19629131619917572
1105	2	0.16566080176079012
1105	3	0.15488966490338285
1105	4	0.1640755253491193
1105	5	0.1122092737805147
1105	6	0.1335446908872342
1105	7	0.09724563727356639
1105	8	0.119233224667596
1105	9	0.11916542075754677
1105	10-11	0.1098972231307016
1105	12-13	0.12371204969231542
1105	14-15	0.2113681339044149
1105	16-17	0.28392234434996766
1105	18-19	0.1839682039329631
1105	20-21	0.20689002521180555
1105	22-23	0.30167298034333356
1105	24-25	0.25558017095907104
1105	26-27	0.25517537243482735
1105	28-29	0.2577007671311051
1105	30-31	0.23929276314422054
1105	32-33	0.3146305836843055
1105	34-35	0.23843414308332456
1105	36-37	0.28633724861228416
1105	38-39	0.22015770035997662
1105	40-41	0.2098308057476359
1105	42-43	0.21784283421074946
1105	44-45	0.22095926916153275
1105	46-47	0.2808818161484652
1105	48-49	0.18402766083936228
1105	50-51	0.227407938716496
1105	52-53	0.2719578965457572
1105	54-55	0.24740372707269387
1105	56-57	0.26629145375572705
1105	58-59	0.19424035911026039
1105	60-61	0.21390664556682282
1105	62-63	0.21002799486479518
1105	64-65	0.20929288522249578
1105	66-67	0.26110657637409673
1105	68-69	0.16421587393547554
1105	70-71	0.2001107562805302
1105	72-73	0.11667815780512569
1105	74-75	0.15659374099769963
1105	76-77	0.13198138998630782
1105	78-79	0.1712046008310395
1105	80-81	0.09878623195736935
1105	82-83	0.15333459320608966
1105	84-85	0.11620405655187227
1105	86-87	0.19984723187162956
1105	88-89	0.23071002649856354
1105	90-91	0.27973404396319523
1105	92-93	0.27974789846095405
1105	94-95	0.23861725914079557
1105	96-97	0.270165575751367
1105	98-99	0.3520137760317432
1105	100	0.31725496036882816
1106	1	0.09908572557621653
1106	2	0.08033616002560251
1106	3	0.0338717235191659
1106	4	0.012163070894551709
1106	5	0.021487350975093022
1106	6	-0.02146362683304659
1106	7	-0.058880748860097754
1106	8	-0.01787068428281202
1106	9	-0.029074148168682257
1106	10-11	-0.011859349424042875
1106	12-13	-0.03232220842515687
1106	14-15	0.012003984672482204
1106	16-17	-0.04461936881735795
1106	18-19	0.006138174964085863
1106	20-21	-0.05518047349057298
1106	22-23	-0.039524923707837445
1106	24-25	0.049147985173668474
1106	26-27	0.04089712616332264
1106	28-29	0.05764818312796649
1106	30-31	0.08845545965427704
1106	32-33	0.04849435219607301
1106	34-35	0.03766897817985182
1106	36-37	-0.003824989067297224
1106	38-39	0.01671431379568844
1106	40-41	-0.02862838437714288
1106	42-43	-0.007067298434151326
1106	44-45	0.028474025309392914
1106	46-47	-0.015241490408413938
1106	48-49	-0.008844515278937592
1106	50-51	-0.039425681057274176
1106	52-53	-0.07710936333423035
1106	54-55	-0.05009819111308644
1106	56-57	-0.03850714646021203
1106	58-59	-0.051109525863417105
1106	60-61	-0.03551541386138979
1106	62-63	-0.058975562448829066
1106	64-65	-0.04757392008365002
1106	66-67	-0.023353528602683582
1106	68-69	-0.03924577077567193
1106	70-71	-0.021216269061127946
1106	72-73	0.04292441407474712
1106	74-75	0.031647651147302724
1106	76-77	0.008824422969155421
1106	78-79	-0.029536363925117826
1106	80-81	-0.022426093869093222
1106	82-83	-0.049043149594318436
1106	84-85	-0.059029534289756214
1106	86-87	-0.0815768889985975
1106	88-89	-0.038732901235256634
1106	90-91	-0.01371479519679042
1106	92-93	-0.009899383851699639
1106	94-95	-0.018135054620159963
1106	96-97	0.001013833049061219
1106	98-99	0.036341125914667316
1106	100	0.10455114165040058
1107	1	0.1267860569488235
1107	2	0.12833689226165745
1107	3	0.1459007971766404
1107	4	0.12080693341928139
1107	5	0.05386312715425845
1107	6	0.11343826146158165
1107	7	0.04243975602484085
1107	8	0.05768538942861312
1107	9	0.10384863186367
1107	10-11	0.09899772469466939
1107	12-13	0.06442146319151476
1107	14-15	0.0678566957994775
1107	16-17	0.05878018534701823
1107	18-19	0.13222802165727643
1107	20-21	0.07692047128313106
1107	22-23	0.03765560699455506
1107	24-25	0.07860647564756817
1107	26-27	0.08401559014479432
1107	28-29	0.08795545637448043
1107	30-31	0.08574458832661236
1107	32-33	0.12181177524672648
1107	34-35	0.15923488952354603
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2216	46-47	-0.3099174886664571
2216	48-49	-0.28638597745343475
2216	50-51	-0.28653439308447304
2216	52-53	-0.33022044562333264
2216	54-55	-0.3044826947136485
2216	56-57	-0.39603827556533133
2216	58-59	-0.4495888309971008
2216	60-61	-0.34691518028203916
2216	62-63	-0.35052281918169825
2216	64-65	-0.34678508730709723
2216	66-67	-0.3264986822131206
2216	68-69	-0.3937991323058796
2216	70-71	-0.3979984341572944
2216	72-73	-0.27740750224220534
2216	74-75	-0.2658085035837914
2216	76-77	-0.34621149625468206
2216	78-79	-0.3608814601922319
2216	80-81	-0.2842055961643979
2216	82-83	-0.2875609256619711
2216	84-85	-0.33015172150765437
2216	86-87	-0.41114638488720345
2216	88-89	-0.3843149283460079
2216	90-91	-0.4230050112859871
2216	92-93	-0.410529657299584
2216	94-95	-0.38127821092616543
2216	96-97	-0.43445303022415516
2216	98-99	-0.41420390693058096
2216	100	-0.48143659863602295
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56738.0
3	9750.0
4	6216.0
5	5077.0
6	4503.0
7	4822.0
8	5549.0
9	6147.0
10	6713.0
11	7438.0
12	7951.0
13	8585.0
14	9279.0
15	9468.0
16	9578.0
17	9683.0
18	9829.0
19	9719.0
20	9792.0
21	10562.0
22	11886.0
23	15302.0
24	22599.0
25	29432.0
26	30781.0
27	29437.0
28	32454.0
29	39698.0
30	49008.0
31	60398.0
32	78532.0
33	106206.0
34	148655.0
35	229706.0
36	428965.0
37	861601.0
38	1239822.0
39	456626.0
40	618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31650279686327	19.34149870155297	13.218431798694716	24.12356670288905
2	15.441501138970615	19.986044204479157	40.192985629654025	24.3794690268962
3	18.275377200983005	25.243654197230253	29.75716268520295	26.723805916583792
4	12.298178536305423	16.440162943379384	38.581210499596565	32.68044802071863
5	13.033997488571035	38.29669197716169	35.45127239193988	13.218038142327387
6	31.4571488884187	37.78334431303401	17.69872992608745	13.060776872459845
7	27.970147380209752	32.28098296622714	22.31718935638042	17.431680297182677
8	25.407795784344877	36.60268745071732	20.07715068632874	17.91236607860906
9	25.46856610435465	16.781728128677916	20.49225186347587	37.257453903491566
10-11	24.244317863474738	26.739165269327025	28.744468723437826	20.27204814376041
12-13	24.74770328123347	24.076460810230476	28.698683566420492	22.477152342115563
14-15	22.05449800264222	25.99633727835606	25.544823101676446	26.40434161732528
16-17	22.051765654360374	29.252134528078134	26.188599561024585	22.50750025653691
18-19	22.031761560873463	28.43346862063956	27.79100409907665	21.743765719410327
20-21	23.080941657193467	27.702227860914153	27.319985522925467	21.89684495896691
22-23	22.750629090498066	27.42059778247285	27.36684036584956	22.461932761179522
24-25	22.61754541178963	27.52565761822955	27.293516678984414	22.563280290996403
26-27	22.387754486547163	27.592678820084256	27.39576138343878	22.623805309929804
28-29	22.42929687533523	27.64496679865147	27.413587794251804	22.512148531761497
30-31	22.600384814278193	27.91230039935454	27.09890393889069	22.388410847476578
32-33	22.366419523254706	27.675076196047137	27.472042036898515	22.486462243799643
34-35	22.49493230093459	27.473358951239142	27.47518559087808	22.556523156948188
36-37	22.755106081180227	27.661290945401145	27.113572710392514	22.470030263026114
38-39	22.48473646613716	27.937694697939374	27.21891750182998	22.358651334093484
40-41	22.34875203156654	27.60274044811289	27.50714741211092	22.54136010820965
42-43	22.363553607374538	27.552432516453102	27.438244254025797	22.645769622146567
44-45	22.342898506842438	27.710297644695803	27.43609750237357	22.51070634608819
46-47	22.50470328455794	27.69259133541409	27.38241420849792	22.420291171530046
48-49	22.46192061158944	27.638381047258413	27.284613143274406	22.615085197877736
50-51	22.38734451087773	27.603455620286294	27.348776772936084	22.660423095899898
52-53	22.460954810219544	27.60720809378863	27.349658842244402	22.58217825374743
54-55	22.495419829923875	27.830505881715528	27.24063047852205	22.433443809838547
56-57	22.35495708892608	27.631019924653415	27.266569371983497	22.747453614437006
58-59	22.30941378350427	27.322611214696884	27.566187265621945	22.801787736176895
60-61	22.541209373797955	27.64938941948087	27.273475007397668	22.53592619932351
62-63	22.334621183045982	27.69688759914602	27.271871452503987	22.696619765304007
64-65	22.38865557574956	27.481356842602928	27.338112476613695	22.791875105033814
66-67	22.302755551436956	27.45691313665305	27.292806559556116	22.947524752353885
68-69	22.491659406915403	27.623656946016013	27.228621059125697	22.656062587942895
70-71	22.434076368006377	27.39958539243812	27.34729365110107	22.819044588454435
72-73	22.341574499678785	27.445539156194158	27.427765429464106	22.785120914662947
74-75	22.377772128301725	27.526506843152593	27.3101447129362	22.785576315609475
76-77	22.35339227057605	27.469302004193953	27.3105364918583	22.8667692333717
78-79	22.439803637376365	27.54199330029243	27.315257917167607	22.7029451451636
80-81	22.371969736529525	27.375846934170013	27.450265103235445	22.801918226065016
82-83	22.274336944890152	27.350929815507836	27.571619267792506	22.80311397180951
84-85	22.232077562014478	27.345000878890374	27.40747932033458	23.01544223876056
86-87	22.39022787160524	27.398318644177493	27.38498213852094	22.826471345696323
88-89	22.18796703099529	27.34163999797264	27.420415976133395	23.049976994898678
90-91	22.230863636145003	27.266456436112346	27.514192042392427	22.988487885350224
92-93	22.314343738102753	27.365689284063016	27.42846297086277	22.891504006971456
94-95	22.132766163561627	27.207193631769925	27.49167458535699	23.16836561931146
96-97	22.16958855063096	27.12197779406002	27.581167393900802	23.127266261408224
98-99	22.110995860855294	27.268748964227374	27.43616188041068	23.184093294506656
100	22.28133031937115	26.984817897134977	27.46556886892076	23.268282914573113
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	443.0
1	421.0
2	381.0
3	327.0
4	264.5
5	233.0
6	199.5
7	184.5
8	202.0
9	214.5
10	210.0
11	220.0
12	247.0
13	278.5
14	318.0
15	353.5
16	434.5
17	563.0
18	752.5
19	1073.5
20	1600.5
21	2321.5
22	3606.0
23	5893.5
24	8920.5
25	12531.0
26	17767.0
27	24935.0
28	33880.0
29	43782.0
30	53535.0
31	64254.0
32	75721.5
33	87707.0
34	99312.0
35	109556.0
36	118947.0
37	128067.0
38	135244.0
39	139374.0
40	144223.5
41	148372.0
42	148599.5
43	149105.0
44	149269.5
45	147268.0
46	146525.5
47	144122.5
48	140986.0
49	137562.0
50	132901.0
51	129536.5
52	122383.5
53	115307.0
54	111835.5
55	106337.0
56	98216.0
57	89138.5
58	81550.0
59	75490.0
60	68863.5
61	60409.5
62	52566.0
63	45178.5
64	36985.5
65	29869.0
66	23652.5
67	17682.0
68	13292.5
69	10501.0
70	7769.0
71	5474.0
72	3982.0
73	2983.0
74	2268.5
75	1687.0
76	1084.5
77	705.5
78	483.0
79	289.0
80	161.5
81	96.0
82	72.5
83	64.0
84	52.5
85	33.5
86	26.0
87	21.0
88	16.0
89	12.5
90	8.0
91	9.5
92	9.5
93	6.5
94	5.0
95	3.5
96	1.5
97	2.0
98	3.5
99	3.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33092881439034105
2	0.11845677688229707
3	0.06498942787975362
4	0.04008212545582693
5	0.04326908344314038
6	0.009462813716176877
7	0.007035822633530475
8	0.015027732663254988
9	0.017773419544632733
10-11	0.020580394079612662
12-13	0.015775442037201607
14-15	0.018067600281923207
16-17	0.018741764471547205
18-19	0.018815309655869826
20-21	0.02336285355315172
22-23	0.01515030797045935
24-25	0.024490546379431863
26-27	0.018778537063708516
28-29	0.018423068672815863
30-31	0.019464958784052952
32-33	0.024355713541507062
34-35	0.014647749210921459
36-37	0.009450556185456439
38-39	0.01302975515582386
40-41	0.009217663101768149
42-43	0.00820028805197193
44-45	0.00864155915790764
46-47	0.008004167560444949
48-49	0.0044004535286366565
50-51	0.006435203628229093
52-53	0.007195170532896149
54-55	0.0031379278644317107
56-57	0.0026476266356142554
58-59	0.00273342935065731
60-61	0.0033830784788404372
62-63	0.0025373088591303283
64-65	0.001997977507431128
66-67	0.0016792817086997826
68-69	0.0031501853951521465
70-71	0.002132810345355928
72-73	0.0017283118315815278
74-75	0.00205926516103331
76-77	0.003763061931173965
78-79	0.0034566236631630555
80-81	0.003628229093249165
82-83	0.0022063555296785464
84-85	0.002892777250022983
86-87	0.0023411883676033463
88-89	0.6028131033003401
90-91	0.6129868537983024
92-93	0.6098979560567523
94-95	0.5974810774369503
96-97	0.5948334508013361
98-99	0.5927987007017436
100	0.5937302730364968
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4079125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.62749256992989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.77141784430928	39.753200875932684
2	14.62801140002161	15.981831681350602
3	5.348605344649328	8.765426961729554
4	2.374175083216207	5.187809268724242
5	1.364380405297214	3.726634022646574
6	0.8164654496515983	2.676087617066829
7	0.5190230721083328	1.9847050310654086
8	0.38823945907243895	1.6966838532666606
9	0.28302016167172744	1.3914613600976382
>10	1.3997618836114267	13.528826698826421
>50	0.0805013624812487	2.989173199605113
>100	0.026174001663412248	2.234605937228127
>500	2.2453224612983513E-4	0.08355349246012608
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.4515061440872736E-5	0.0
4	0.0	0.0	0.0	2.4515061440872736E-5	0.0
5	0.0	0.0	0.0	2.4515061440872736E-5	0.0
6	0.0	0.0	0.0	2.4515061440872736E-5	0.0
7	0.0	0.0	0.0	4.903012288174547E-5	0.0
8	0.0	0.0	0.0	4.903012288174547E-5	0.0
9	0.0	0.0	0.0	7.35451843226182E-5	0.0
10-11	0.0	0.0	0.0	7.35451843226182E-5	0.0
12-13	0.0	0.0	0.0	9.806024576349094E-5	0.0
14-15	0.0	0.0	0.0	1.3483283792480006E-4	0.0
16-17	0.0	0.0	0.0	1.470903686452364E-4	0.0
18-19	0.0	2.4515061440872736E-5	0.0	1.470903686452364E-4	0.0
20-21	0.0	2.4515061440872736E-5	0.0	1.7160543008610916E-4	0.0
22-23	0.0	2.4515061440872736E-5	0.0	2.2063555296785462E-4	0.0
24-25	0.0	2.4515061440872736E-5	0.0	3.432108601722183E-4	0.0
26-27	0.0	2.4515061440872736E-5	0.0	4.535286366561456E-4	0.0
28-29	0.0	4.903012288174547E-5	0.0	0.0013605859099684368	0.0
30-31	0.0	4.903012288174547E-5	0.0	0.00519719302546502	0.0
32-33	0.0	4.903012288174547E-5	0.0	0.014721294395244078	0.0
34-35	0.0	4.903012288174547E-5	0.0	0.02913615052247725	0.0
36-37	0.0	4.903012288174547E-5	0.0	0.05411699813072657	0.0
38-39	0.0	4.903012288174547E-5	0.0	0.09472619740753226	0.0
40-41	0.0	4.903012288174547E-5	0.0	0.1414641620445561	0.0
42-43	0.0	4.903012288174547E-5	0.0	0.18970980296019369	0.0
44-45	0.0	4.903012288174547E-5	0.0	0.2456041430453835	0.0
46-47	0.0	9.806024576349094E-5	0.0	0.3150062819844942	0.0
48-49	0.0	9.806024576349094E-5	0.0	0.3919222872552324	0.0
50-51	0.0	1.2257530720436368E-4	0.0	0.4730548830938007	0.0
52-53	0.0	1.470903686452364E-4	0.0	0.5579873134557043	0.0
54-55	0.0	1.470903686452364E-4	0.0	0.6488401311555787	0.0
56-57	0.0	1.470903686452364E-4	0.0	0.758201820243312	0.0
58-59	0.0	1.7160543008610916E-4	0.0	0.8784481966107928	0.0
60-61	0.0	1.7160543008610916E-4	0.0	1.009407654827935	0.0
62-63	0.0	1.7160543008610916E-4	0.0	1.1412986853798301	0.0
64-65	0.0	1.7160543008610916E-4	0.0	1.280421659056783	0.0
66-67	0.0	1.7160543008610916E-4	0.0	1.4404192075506388	0.0
68-69	0.0	1.7160543008610916E-4	0.0	1.6170011951092453	0.0
70-71	0.0	1.838629608065455E-4	0.0	1.8103392271626881	0.0
72-73	0.0	1.961204915269819E-4	0.0	2.011350473447124	0.0
74-75	0.0	2.0837802224741827E-4	0.0	2.2191769068121228	0.0
76-77	0.0	2.4515061440872737E-4	0.0	2.4457451046486685	0.0
78-79	0.0	2.4515061440872737E-4	0.0	2.7028713265712625	0.0
80-81	0.0	2.8192320657003647E-4	0.0	2.9820365887292004	0.0
82-83	0.0	2.941807372904728E-4	0.0	3.270480801642509	0.0
84-85	0.0	2.941807372904728E-4	0.0	3.55814053258971	0.0
86-87	0.0	2.941807372904728E-4	0.0	3.863451107774339	0.0
88	0.0	2.941807372904728E-4	0.0	4.11600527073821	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	12565	0.0	27.779474	1
GTATCAA	6115	0.0	27.5775	1
TACATGG	12600	0.0	26.451988	2
TCAACGC	6225	0.0	25.733839	4
GGTATCA	4345	0.0	25.368475	1
ATCAACG	6345	0.0	25.325844	3
ACATGGG	12860	0.0	24.917984	3
CAACGCA	6670	0.0	24.016964	5
AACGCAG	6865	0.0	23.326754	6
TATCAAC	7365	0.0	22.275902	2
CATGGGG	9220	0.0	20.278774	4
ACGCAGA	8200	0.0	19.128155	7
GAGTACA	8380	0.0	18.381123	1
GTATAGA	1500	0.0	18.213987	1
GTATAGG	1430	0.0	17.787954	1
CGCAGAG	8670	0.0	17.657898	8
AGTACAT	8705	0.0	16.41676	2
TCTATAC	1415	0.0	15.606694	3
GTATTAG	1995	0.0	15.583655	1
TATACCG	275	1.2539569E-5	15.374443	5
>>END_MODULE