FastQCFastQC Report
Wed 25 May 2016
SRR1033141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4079125
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51280.1257132350687954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43470.10656697208347378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.042.0300641
GTATCAA99750.038.825131
TCAACGC113050.033.475344
ATCAACG114950.032.962933
CAACGCA116750.032.374195
AACGCAG121750.031.0339956
TATCAAC123900.030.810242
GTACATG121600.027.2436351
ACGCAGA141650.026.5410867
CGCAGAG146500.025.468088
TACATGG124500.025.3374862
ACATGGG125050.023.8720513
GCAGAGT160450.023.1073789
CATGGGG88150.019.9990184
AGAGTAC138600.019.7151410-11
GAGTACT91850.019.62002612-13
CAGAGTA157400.018.55452210-11
GTACTTT98650.018.1723414-15
GAGTACA85550.017.9867941
AGTACTT97050.017.74564612-13
Produced by FastQC (version 0.11.5)