FastQCFastQC Report
Wed 25 May 2016
SRR1033140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241159
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG87350.027.5038621
TACATGG89600.025.6543252
ACATGGG90400.024.9477483
GTATCAA52350.024.7458521
TCAACGC51150.023.8769594
CAACGCA51550.023.691325
ATCAACG52400.023.6717933
AACGCAG54300.022.6581976
GGTATCA38200.020.8405761
TATCAAC61750.020.5535342
CATGGGG65250.020.0851444
CCTATAC10500.019.6889213
TACGTCG2451.9777872E-719.172495
GAGTACA59650.018.874431
ACGCAGA62400.018.8139217
ACGTCGC2251.8627343E-618.783826
CGCAGAG62900.018.6643688
CCGTCGT1550.001039869918.177899
GTATAGG11150.017.744381
AGTACAT63650.016.7643682
Produced by FastQC (version 0.11.5)