##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033140_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3241159 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.032504113497673 33.0 31.0 34.0 28.0 34.0 2 31.1519931604713 33.0 31.0 34.0 28.0 34.0 3 31.35953620294469 34.0 31.0 34.0 28.0 34.0 4 34.73508519637574 37.0 35.0 37.0 32.0 37.0 5 34.837701883801444 37.0 35.0 37.0 32.0 37.0 6 34.90926980132724 37.0 35.0 37.0 32.0 37.0 7 34.82068266320782 37.0 35.0 37.0 32.0 37.0 8 34.83154359289377 37.0 35.0 37.0 32.0 37.0 9 36.440278307852225 39.0 37.0 39.0 32.0 39.0 10-11 36.4174620868646 39.0 37.0 39.0 32.0 39.0 12-13 36.354588127271754 39.0 37.0 39.0 32.0 39.0 14-15 37.71171994339062 40.0 38.0 41.0 32.0 41.0 16-17 37.69879108059802 40.0 38.0 41.0 32.0 41.0 18-19 37.667920487702084 40.0 38.0 41.0 32.0 41.0 20-21 37.674659589362946 40.0 38.0 41.0 32.0 41.0 22-23 37.61862963217787 40.0 38.0 41.0 32.0 41.0 24-25 37.592540662152025 40.0 38.0 41.0 32.0 41.0 26-27 37.52718657122344 40.0 38.0 41.0 32.0 41.0 28-29 37.4848470871068 40.0 38.0 41.0 31.5 41.0 30-31 37.47656424754231 40.0 38.0 41.0 31.0 41.0 32-33 37.54380099834658 40.0 38.0 41.0 31.5 41.0 34-35 37.50698361296067 40.0 38.0 41.0 31.0 41.0 36-37 37.4285038160732 40.0 38.0 41.0 31.0 41.0 38-39 37.34406642808946 40.0 38.0 41.0 31.0 41.0 40-41 37.273932256948825 40.0 38.0 41.0 30.5 41.0 42-43 37.15302427310725 40.0 38.0 41.0 30.0 41.0 44-45 37.02317751150129 40.0 37.5 41.0 30.0 41.0 46-47 36.88352499831079 40.0 37.0 41.0 30.0 41.0 48-49 36.755725189662094 40.0 37.0 41.0 30.0 41.0 50-51 36.62077624084471 40.0 37.0 41.0 30.0 41.0 52-53 36.45861588400939 40.0 36.0 41.0 30.0 41.0 54-55 36.29113489958376 40.0 36.0 41.0 29.5 41.0 56-57 36.11932521668946 39.5 35.0 41.0 29.0 41.0 58-59 35.9074602634428 39.0 35.0 41.0 29.0 41.0 60-61 35.66732224491301 39.0 35.0 41.0 28.0 41.0 62-63 35.39859923564379 39.0 35.0 41.0 28.0 41.0 64-65 35.117120604080206 38.0 35.0 40.0 28.0 41.0 66-67 34.80864514823247 37.5 34.0 40.0 28.0 41.0 68-69 34.48914724640167 37.0 34.0 40.0 27.0 41.0 70-71 34.13441441780548 36.5 34.0 39.0 26.5 41.0 72-73 33.76172181000685 36.0 34.0 39.0 26.0 41.0 74-75 33.38328357232706 35.5 33.5 39.0 26.0 40.0 76-77 33.04920215268674 35.0 33.0 37.5 26.0 40.0 78-79 32.678338057466476 35.0 33.0 37.0 25.0 39.0 80-81 32.317756703697654 35.0 33.0 37.0 25.0 39.0 82-83 32.09638959396932 35.0 33.0 36.0 25.0 38.5 84-85 31.824296956736774 35.0 33.0 36.0 24.0 37.0 86-87 31.568223126356962 35.0 33.0 35.5 24.0 37.0 88-89 31.207352987002487 35.0 33.0 35.0 20.5 36.5 90-91 31.010472334124923 35.0 32.0 35.0 20.0 36.0 92-93 30.81786839213997 35.0 32.0 35.0 18.5 36.0 94-95 30.632230631079807 35.0 32.0 35.0 17.0 36.0 96-97 30.434607496886144 35.0 32.0 35.0 6.0 35.0 98-99 30.214472970934164 35.0 32.0 35.0 2.0 35.0 100 30.0344602038962 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11101705704112064 1101 2 0.01302511018488417 1101 3 0.0663130256150346 1101 4 0.0015848929250736887 1101 5 0.08358497987415348 1101 6 0.010049377113709568 1101 7 0.09322046631027092 1101 8 0.05759189070987958 1101 9 0.05666074926271136 1101 10-11 0.05754614436339267 1101 12-13 0.11832701737414197 1101 14-15 0.16010713173188407 1101 16-17 0.061311523347576724 1101 18-19 0.10464661667496244 1101 20-21 0.03103890940203513 1101 22-23 0.09363142840555128 1101 24-25 0.07549497084869472 1101 26-27 0.10655929510166118 1101 28-29 0.13568868320256655 1101 30-31 0.10522348970655315 1101 32-33 0.1463023666799188 1101 34-35 0.13811264153567748 1101 36-37 0.10264232847200816 1101 38-39 0.09813246769708428 1101 40-41 0.09511073535608716 1101 42-43 0.06901775289370704 1101 44-45 -0.022650269465479766 1101 46-47 0.09446821102957159 1101 48-49 0.06724104555291888 1101 50-51 0.09489781380744233 1101 52-53 0.06942175898018377 1101 54-55 0.06916866773329389 1101 56-57 0.04453005074589811 1101 58-59 0.09207764885578484 1101 60-61 0.09818487877780058 1101 62-63 -0.02411853717629242 1101 64-65 0.036075065494294734 1101 66-67 -0.029519638724657682 1101 68-69 0.01144884805026436 1101 70-71 -0.031538896660840976 1101 72-73 -0.027580105370041963 1101 74-75 -0.06044859700012495 1101 76-77 -0.03665733228412904 1101 78-79 -0.025559405166283966 1101 80-81 -0.008178674519086826 1101 82-83 0.0540815036171054 1101 84-85 -0.05044150010748183 1101 86-87 -0.005992913895301655 1101 88-89 0.1294668672696382 1101 90-91 0.1938486503734147 1101 92-93 0.1901536033029707 1101 94-95 0.1763229490228646 1101 96-97 0.11984194492783473 1101 98-99 0.1372817460103235 1101 100 0.1054170527863576 1102 1 0.18955547369348835 1102 2 0.15082398426491395 1102 3 0.2366366709169867 1102 4 0.22491723357028093 1102 5 0.0990043049419711 1102 6 0.16991436649018254 1102 7 0.21296605344624453 1102 8 0.16862012034131624 1102 9 0.2835543900370965 1102 10-11 0.24156737403186668 1102 12-13 0.29096716591455163 1102 14-15 0.3004814362518218 1102 16-17 0.32289446470768013 1102 18-19 0.3645771196881711 1102 20-21 0.312575685095581 1102 22-23 0.3141090978358676 1102 24-25 0.35365907920186146 1102 26-27 0.3849784096641713 1102 28-29 0.2938696300909811 1102 30-31 0.3305578511271676 1102 32-33 0.3521713643496227 1102 34-35 0.4121273009441282 1102 36-37 0.40605209879068127 1102 38-39 0.3463337243797753 1102 40-41 0.3863085993885562 1102 42-43 0.3892788134927798 1102 44-45 0.3482487507211047 1102 46-47 0.3927055786323166 1102 48-49 0.3889948231217346 1102 50-51 0.3267678076925833 1102 52-53 0.3406900232448393 1102 54-55 0.3216394596489778 1102 56-57 0.2470868493871663 1102 58-59 0.2942866602215588 1102 60-61 0.25369525211671373 1102 62-63 0.33961616906486114 1102 64-65 0.397010419577839 1102 66-67 0.3091487791295222 1102 68-69 0.24781669228691072 1102 70-71 0.293179670587854 1102 72-73 0.3313528027702759 1102 74-75 0.2879684937635645 1102 76-77 0.18516296987602487 1102 78-79 0.3052751539101237 1102 80-81 0.3134883688147667 1102 82-83 0.2560669711921051 1102 84-85 0.32861323089545635 1102 86-87 0.2746662681800771 1102 88-89 0.41931274488026204 1102 90-91 0.3449758741900695 1102 92-93 0.44867018711545015 1102 94-95 0.38994063018403935 1102 96-97 0.3083536553722972 1102 98-99 0.4122377742097214 1102 100 0.3917710743448204 1103 1 0.19503833535079096 1103 2 0.12522142735461728 1103 3 0.21800824933265517 1103 4 0.26667841067838083 1103 5 0.12845231862353046 1103 6 0.1924168277442675 1103 7 0.2562455247122557 1103 8 0.2792009211624489 1103 9 0.29834424783211944 1103 10-11 0.2514395946896357 1103 12-13 0.3062588787488778 1103 14-15 0.3258003699314074 1103 16-17 0.31983407262750774 1103 18-19 0.3512920458064386 1103 20-21 0.31813721865356115 1103 22-23 0.29781477780004906 1103 24-25 0.3177953076856497 1103 26-27 0.34582112541818333 1103 28-29 0.3108711241218387 1103 30-31 0.3741549050483002 1103 32-33 0.34134782027607713 1103 34-35 0.37646618309729973 1103 36-37 0.4410803030347026 1103 38-39 0.3982365917867412 1103 40-41 0.3972814914043852 1103 42-43 0.3514356992325034 1103 44-45 0.32852705624600986 1103 46-47 0.29583588393075644 1103 48-49 0.32084790849398814 1103 50-51 0.2018978093321948 1103 52-53 0.13466527210906776 1103 54-55 0.25964526666459875 1103 56-57 0.18200349761128365 1103 58-59 0.2018992878703898 1103 60-61 0.16089875831011113 1103 62-63 0.1990984123757329 1103 64-65 0.23026201746296238 1103 66-67 0.22591648159385613 1103 68-69 0.1811356851542385 1103 70-71 0.1968581517643102 1103 72-73 0.18434355735144692 1103 74-75 0.20010291757277088 1103 76-77 0.14307382468666674 1103 78-79 0.12710172611659232 1103 80-81 0.1930707772631024 1103 82-83 0.19808247176229798 1103 84-85 0.24551015379478258 1103 86-87 0.2114215044567942 1103 88-89 0.28198758766513166 1103 90-91 0.30896730409965656 1103 92-93 0.3601100740631935 1103 94-95 0.33543887881518586 1103 96-97 0.32746899720170575 1103 98-99 0.32241583188137213 1103 100 0.3417157099137853 1104 1 0.058461623301326426 1104 2 -0.07195450686559468 1104 3 0.06446122320795311 1104 4 0.017978709237318924 1104 5 0.009375704633136195 1104 6 -0.06283556039154092 1104 7 -0.08517529639019727 1104 8 -0.018927131257278518 1104 9 0.02211667199712508 1104 10-11 0.0788194175613981 1104 12-13 0.0863949255444254 1104 14-15 0.057720839964737536 1104 16-17 0.12507882805760318 1104 18-19 0.11416790797532173 1104 20-21 0.05160008713038167 1104 22-23 0.04465994818783514 1104 24-25 4.685930742809319E-4 1104 26-27 0.0406312742312025 1104 28-29 0.14757216800035877 1104 30-31 0.08897813635426388 1104 32-33 0.0806669352214513 1104 34-35 0.02769400187141713 1104 36-37 0.0654792274592566 1104 38-39 0.01963168431033324 1104 40-41 0.07317374940256371 1104 42-43 -0.022195537544980937 1104 44-45 -0.02745012532086122 1104 46-47 -0.08914739037277997 1104 48-49 -0.21995175259267796 1104 50-51 -0.1657961055638424 1104 52-53 -0.13396570759464765 1104 54-55 -0.17463130654735437 1104 56-57 -0.13028123623521282 1104 58-59 -0.19488422456649346 1104 60-61 -0.13528650989645996 1104 62-63 -0.1283129484120238 1104 64-65 -0.15239856000069807 1104 66-67 -0.08977832254773688 1104 68-69 -0.17537070617670025 1104 70-71 -0.060365753657983134 1104 72-73 -0.013534360524175781 1104 74-75 -0.01561037644506058 1104 76-77 -0.09241853518692977 1104 78-79 -0.08305062498645555 1104 80-81 -0.05367695244864734 1104 82-83 -0.04776406420841539 1104 84-85 -0.014767319793755007 1104 86-87 -0.01984837458955724 1104 88-89 -0.02826827317509384 1104 90-91 -0.032057393710569926 1104 92-93 -0.08554249930707414 1104 94-95 0.00757509911344556 1104 96-97 0.045651082817343536 1104 98-99 -0.0077136423064771975 1104 100 -0.039809237549523147 1105 1 0.07444635090318741 1105 2 0.030736139148029196 1105 3 0.03886251621062087 1105 4 0.03211843629146216 1105 5 0.017932552720388628 1105 6 0.0030089100584476114 1105 7 -0.0784892813266751 1105 8 0.03492515481470804 1105 9 0.011666563019822718 1105 10-11 0.06521155437699377 1105 12-13 0.11115980107147294 1105 14-15 0.12955953006838428 1105 16-17 0.19803780497920087 1105 18-19 0.18227401808363197 1105 20-21 0.19533764381394292 1105 22-23 0.21729072499124413 1105 24-25 0.21982548868988516 1105 26-27 0.23839805029649597 1105 28-29 0.25886613502347444 1105 30-31 0.29008511114832203 1105 32-33 0.27353300970732874 1105 34-35 0.23348848584196702 1105 36-37 0.25022588236640075 1105 38-39 0.24115286902598143 1105 40-41 0.1981240527405248 1105 42-43 0.21525898644504338 1105 44-45 0.26357252750504045 1105 46-47 0.24691216288965023 1105 48-49 0.22770020301086902 1105 50-51 0.2505057358959135 1105 52-53 0.1853227170643592 1105 54-55 0.1277901497454934 1105 56-57 0.11673243165549962 1105 58-59 0.1109694400319512 1105 60-61 0.10599792336838476 1105 62-63 0.20178100519693487 1105 64-65 0.14715232091226937 1105 66-67 0.11377990738547794 1105 68-69 0.12035245717893872 1105 70-71 0.1235892018237692 1105 72-73 0.14232047866461528 1105 74-75 0.14079183886637026 1105 76-77 0.13921425518776687 1105 78-79 0.12396226239748387 1105 80-81 0.09998074315659977 1105 82-83 0.15151003895993398 1105 84-85 0.11953314824644679 1105 86-87 0.18570714833849067 1105 88-89 0.16247943188390934 1105 90-91 0.2437703371247295 1105 92-93 0.21773517376476903 1105 94-95 0.26627372791540793 1105 96-97 0.2811379977532553 1105 98-99 0.3328735311499571 1105 100 0.41690642740862316 1106 1 0.0375613651315696 1106 2 0.037878686586552135 1106 3 0.019381395092224807 1106 4 0.06584961919174503 1106 5 -0.019779543819943513 1106 6 0.06523123156400601 1106 7 -0.0210354998708695 1106 8 0.06546585313228093 1106 9 0.0745829519633503 1106 10-11 0.0911890065823755 1106 12-13 0.11844548961522605 1106 14-15 0.08966614937800443 1106 16-17 0.05879101982208823 1106 18-19 0.1002008732673687 1106 20-21 0.11251553263885228 1106 22-23 0.15184923298035358 1106 24-25 0.20179346003298093 1106 26-27 0.19018060012221127 1106 28-29 0.13796630544093347 1106 30-31 0.1775288047213408 1106 32-33 0.17737771467344032 1106 34-35 0.17449518532379216 1106 36-37 0.12201654173540533 1106 38-39 0.149182833615356 1106 40-41 0.16253001591735483 1106 42-43 0.15687340708915087 1106 44-45 0.149506871692914 1106 46-47 0.13645021732136087 1106 48-49 0.11305435792770879 1106 50-51 0.1229030860420508 1106 52-53 0.11510283452167158 1106 54-55 0.0924053223234722 1106 56-57 0.08885771066319137 1106 58-59 0.1642344997703873 1106 60-61 0.131630155332644 1106 62-63 0.10918045650447539 1106 64-65 0.1347715014411861 1106 66-67 0.19455925939026741 1106 68-69 0.16518505091554658 1106 70-71 0.21856396398202094 1106 72-73 0.249018715782789 1106 74-75 0.2923874191240614 1106 76-77 0.18840039105055695 1106 78-79 0.21750952896702103 1106 80-81 0.16114234497192825 1106 82-83 0.07345848738948746 1106 84-85 0.11277740739375375 1106 86-87 0.1765697752469073 1106 88-89 0.2301729336816436 1106 90-91 0.25222872761696635 1106 92-93 0.22764888024480356 1106 94-95 0.1905911142387815 1106 96-97 0.08438879696119272 1106 98-99 0.1383438624047706 1106 100 0.219365272589215 1107 1 -0.0387654905213779 1107 2 -0.03495920873793423 1107 3 -0.011378191923714809 1107 4 -0.08686165860224548 1107 5 -0.05487354406649558 1107 6 -0.08160083908580162 1107 7 -0.04143263997413271 1107 8 -0.09548829104805634 1107 9 -0.041723949436196506 1107 10-11 -0.01135822668118891 1107 12-13 -0.012415912637358417 1107 14-15 -0.09025882735587487 1107 16-17 -0.04617220948383505 1107 18-19 -0.039924102700020114 1107 20-21 -3.5526420909803846E-4 1107 22-23 -0.05742666842621702 1107 24-25 -0.06238685864743587 1107 26-27 0.009881361373146547 1107 28-29 -0.01430024927581286 1107 30-31 -0.05045129499882961 1107 32-33 0.05508789064005981 1107 34-35 -0.004550844552660749 1107 36-37 -0.037688625228156525 1107 38-39 0.02503479368640882 1107 40-41 -0.02491305633505192 1107 42-43 0.039824728355824845 1107 44-45 0.001823918846660888 1107 46-47 -0.002571853398713131 1107 48-49 -0.0874018145731057 1107 50-51 -0.04244351691237824 1107 52-53 -0.036745704455235284 1107 54-55 -0.09103440388679473 1107 56-57 -0.02509475331024902 1107 58-59 -5.930067591322086E-4 1107 60-61 0.05900132776000788 1107 62-63 0.045140133774452806 1107 64-65 0.029347021647424754 1107 66-67 0.046315332694682354 1107 68-69 0.04318653247415227 1107 70-71 0.08589760082355014 1107 72-73 0.07645686015218445 1107 74-75 0.09689310263708961 1107 76-77 0.08770941753977013 1107 78-79 0.05825427484730028 1107 80-81 0.04558859847142571 1107 82-83 0.03532971123545536 1107 84-85 0.0477644824796144 1107 86-87 -0.02390663734368914 1107 88-89 0.1121373210241039 1107 90-91 0.12375521682448465 1107 92-93 0.17001628250632805 1107 94-95 0.11752534823881078 1107 96-97 0.13708117602046954 1107 98-99 0.20439721576049052 1107 100 0.2489756855924412 1108 1 -0.02741066864181363 1108 2 -0.020377719042553366 1108 3 0.018329875861628153 1108 4 -0.010414656107464282 1108 5 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64-65 0.10153334625655219 2214 66-67 0.16445272405395173 2214 68-69 0.14778195531587812 2214 70-71 0.24750351060441034 2214 72-73 0.22803918864801176 2214 74-75 0.16264070130522157 2214 76-77 0.1855027785797816 2214 78-79 0.18993628467632107 2214 80-81 0.24336326729684288 2214 82-83 0.11128375616636532 2214 84-85 0.13213866830464838 2214 86-87 0.154493792292147 2214 88-89 0.06471050502830522 2214 90-91 -0.013233660394057267 2214 92-93 0.0800837762407447 2214 94-95 0.029165890153674212 2214 96-97 0.0011643049846128406 2214 98-99 -0.08342659377863271 2214 100 0.017598964877958423 2215 1 -0.1887729391895796 2215 2 -0.15258299047333423 2215 3 -0.13728774414236966 2215 4 -0.25259627186506606 2215 5 -0.23104182111971028 2215 6 -0.14189373468573763 2215 7 -0.05502995100491148 2215 8 -0.08630780748983113 2215 9 -0.19615404484898136 2215 10-11 -0.24421712446450528 2215 12-13 -0.1942285925983498 2215 14-15 -0.30014760360950987 2215 16-17 -0.2042168002377167 2215 18-19 -0.2402054063100607 2215 20-21 -0.19315662103056752 2215 22-23 -0.25814107135726516 2215 24-25 -0.12474355907364298 2215 26-27 -0.20172861006587084 2215 28-29 -0.17532939917327184 2215 30-31 -0.24071108476017145 2215 32-33 -0.15127557061639152 2215 34-35 -0.19604592028466783 2215 36-37 -0.34197094022768226 2215 38-39 -0.2325734680504823 2215 40-41 -0.23853547752464976 2215 42-43 -0.27539243523787604 2215 44-45 -0.16752562620265365 2215 46-47 -0.06752700426915936 2215 48-49 -0.03294841297582707 2215 50-51 -0.12009050008536803 2215 52-53 -0.28815789629567945 2215 54-55 -0.1565182356213839 2215 56-57 -0.2484737659892815 2215 58-59 -0.2354192549347971 2215 60-61 -0.21293689135653437 2215 62-63 -0.14626576161366245 2215 64-65 -0.24177751775719258 2215 66-67 -0.19648593683769633 2215 68-69 -0.18932975566826826 2215 70-71 -0.1639752480825436 2215 72-73 -0.07098152539553126 2215 74-75 -0.09177191562378795 2215 76-77 -0.2744643249737422 2215 78-79 -0.19077230642348297 2215 80-81 -0.23448321716472975 2215 82-83 -0.22280359432054553 2215 84-85 -0.22942708446412396 2215 86-87 -0.2542823062690118 2215 88-89 -0.2012137475106286 2215 90-91 -0.21213956608175266 2215 92-93 -0.23054385152314438 2215 94-95 -0.30748765783418364 2215 96-97 -0.34847495434984666 2215 98-99 -0.47266926593091796 2215 100 -0.3622605721966572 2216 1 -0.04527911061534695 2216 2 -0.028667478125544932 2216 3 -0.13298208841939285 2216 4 -0.14438030482220654 2216 5 -9.915314470276826E-5 2216 6 -0.037339323726122586 2216 7 0.05716258828414311 2216 8 0.017129961957401463 2216 9 -0.1460681672474422 2216 10-11 -0.01629676069153163 2216 12-13 -0.08401619519807468 2216 14-15 -0.11193979560867717 2216 16-17 -0.13766997956346216 2216 18-19 -0.2220955202108783 2216 20-21 -0.17431183236745085 2216 22-23 -0.15250680896540558 2216 24-25 -0.21275662468189438 2216 26-27 -0.20508010351395 2216 28-29 -0.18063080212517235 2216 30-31 -0.2359154704902835 2216 32-33 -0.2061036313747806 2216 34-35 -0.20581189729804095 2216 36-37 -0.2273882444480506 2216 38-39 -0.1875979035739519 2216 40-41 -0.24093710998177187 2216 42-43 -0.28348507177825866 2216 44-45 -0.24867142759953964 2216 46-47 -0.18700697622197282 2216 48-49 -0.07006576088595295 2216 50-51 -0.06304600887366973 2216 52-53 -0.055494044878109605 2216 54-55 -0.08935560858895997 2216 56-57 -0.1723478449856728 2216 58-59 -0.18036569407811953 2216 60-61 -0.2033432653798286 2216 62-63 -0.05136251511737555 2216 64-65 -0.06540631427487398 2216 66-67 -0.11833703738818002 2216 68-69 -0.1054720123841193 2216 70-71 -0.24158583479741225 2216 72-73 -0.060876822272966535 2216 74-75 -0.03679498460820696 2216 76-77 -0.08936738531610189 2216 78-79 -0.1575085916034027 2216 80-81 -0.12634366842441125 2216 82-83 -0.1931283842698157 2216 84-85 -0.15030932081022996 2216 86-87 -0.22641518675001038 2216 88-89 -0.1809105591733413 2216 90-91 -0.16696355119733397 2216 92-93 -0.13630383127749468 2216 94-95 -0.06736980476998511 2216 96-97 -0.1789942843198098 2216 98-99 -0.1456624983895054 2216 100 -0.19406425849569686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59493.0 3 9821.0 4 6326.0 5 4893.0 6 4250.0 7 4512.0 8 5035.0 9 5559.0 10 6189.0 11 6528.0 12 7404.0 13 7841.0 14 8335.0 15 8328.0 16 8937.0 17 9055.0 18 8866.0 19 8892.0 20 9120.0 21 9562.0 22 10876.0 23 14409.0 24 20188.0 25 25240.0 26 25758.0 27 24759.0 28 27822.0 29 34179.0 30 41669.0 31 51082.0 32 66312.0 33 87729.0 34 120820.0 35 182216.0 36 330585.0 37 666121.0 38 966474.0 39 345506.0 40 468.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39307825656265 19.442391035165922 13.24724492322932 23.9172857850421 2 15.436834332275751 19.816746090903504 40.47608824384348 24.27033133297727 3 18.549350642133515 25.027122183507117 29.542958019120448 26.88056915523892 4 12.209285098754794 16.34420224547091 38.751564841861466 32.69494781391283 5 13.091038840621222 37.966463403341685 35.70895029452399 13.233547461513103 6 31.491445228573113 37.82860094488345 17.620246462086943 13.059707364456496 7 27.701546253221682 32.245364063597776 22.30220266954156 17.750887013638984 8 25.178746328641445 36.577901923887545 20.166134567827328 18.077217179643686 9 24.94094390388337 17.085086758072592 20.742506780457592 37.23146255758644 10-11 24.069298228830615 26.857823801450287 28.76880366911097 20.304074300608125 12-13 24.69168336999554 24.153144697706903 28.72066361344575 22.4345083188518 14-15 21.83521525604883 26.17736727495811 25.69671977483892 26.29069769415414 16-17 21.810891412369333 29.46078041091215 26.325845595337505 22.402482581381012 18-19 21.76371193824175 28.675548097187892 27.950337393243927 21.610402571326425 20-21 22.916727745516145 27.880439965225946 27.48464902061415 21.71818326864376 22-23 22.383097757075387 27.66939195558895 27.542919828984136 22.404590458351525 24-25 22.39096088298782 27.707031087858237 27.554823734039562 22.34718429511438 26-27 22.201891173290278 27.674240123831673 27.609867898070934 22.514000804807118 28-29 22.164342565550886 27.82599853851338 27.657554182394517 22.352104713541216 30-31 22.41720945891942 28.23259827119112 27.22664146888579 22.12355080100367 32-33 22.058229224354612 27.852821076472665 27.76119416803523 22.327755531137488 34-35 22.213071135674696 27.631813832955913 27.784884527362408 22.370230504006976 36-37 22.470798075622074 27.86466284430315 27.360410782453126 22.304128297621652 38-39 22.305672921226186 28.147971003200695 27.43395914928939 22.112396926283733 40-41 22.175567413652733 27.828821902828093 27.71959040657413 22.27602027694504 42-43 22.069613811495724 27.781205360111365 27.580981068174538 22.568199760218373 44-45 22.196124884810615 27.838765561655965 27.623498526702107 22.341611026831316 46-47 22.332443917840532 27.95271672849377 27.535347130458664 22.179492223207035 48-49 22.253203499941606 27.88109812773766 27.517108396709624 22.348589975611112 50-51 22.153557587296454 27.785156436215452 27.613015737498237 22.448270238989853 52-53 22.255846107752834 27.743428788827362 27.588904621747485 22.41182048167232 54-55 22.28925290638567 28.107840260557293 27.349830440909173 22.25307639214786 56-57 22.13083103484077 27.823883043689072 27.52242428318889 22.522861638281267 58-59 22.102666387540783 27.48102825902738 27.798037716242213 22.618267637189625 60-61 22.24303514780665 27.880729080451456 27.551945879125583 22.32428989261631 62-63 22.141902432281665 27.8504457784338 27.528422694713356 22.479229094571178 64-65 22.131643983968356 27.704367974971383 27.55292201080501 22.61106603025525 66-67 22.000125574398744 27.73688553585018 27.466946859043357 22.79604203070772 68-69 22.310193977875485 27.691418146285535 27.53386193521853 22.464525940620454 70-71 22.26735612469422 27.597435003863662 27.555134504634466 22.580074366807654 72-73 22.129690337091827 27.708482138036256 27.6570644888086 22.50476303606332 74-75 22.07882244717024 27.82854680725909 27.482583429764233 22.610047315806437 76-77 22.15553241141775 27.677399379181043 27.460416659274493 22.70665155012671 78-79 22.235923179335124 27.75330872200564 27.52728663968985 22.48348145896938 80-81 22.2452942777257 27.480119121742497 27.66964080203539 22.604945798496416 82-83 22.120521323892284 27.576440612885555 27.82951870116715 22.473519362055008 84-85 22.016026504839374 27.521618256448455 27.664842628287555 22.797512610424615 86-87 22.197916255282177 27.670785242486474 27.551550590419023 22.57974791181233 88-89 22.00432169381344 27.535409999864974 27.629662842388232 22.830605463933352 90-91 22.021887982874052 27.42552643939067 27.71520663481421 22.837378942921067 92-93 22.23579632716901 27.56744985854803 27.62011310880339 22.57664070547957 94-95 21.955287798617398 27.32734760008101 27.77613016215176 22.94123443914983 96-97 21.93549017864251 27.353985551610137 27.798632569199082 22.911891700548267 98-99 21.885392042406398 27.498516821947096 27.729167885513395 22.88692325013311 100 22.108080056910378 27.197952269276467 27.637505478120417 23.05646219569274 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 337.0 1 311.5 2 265.5 3 220.0 4 161.5 5 143.0 6 139.5 7 135.0 8 151.5 9 156.0 10 158.0 11 162.0 12 175.5 13 197.0 14 227.5 15 276.0 16 355.5 17 495.5 18 704.5 19 994.0 20 1467.5 21 2186.0 22 3326.5 23 5185.5 24 7796.5 25 11089.0 26 15479.0 27 21085.0 28 28437.0 29 37062.5 30 45636.5 31 54075.0 32 62806.5 33 72389.5 34 81952.0 35 89549.0 36 96256.5 37 103857.5 38 109997.0 39 113246.0 40 115774.0 41 118705.5 42 119968.5 43 119828.0 44 118643.5 45 116839.0 46 114709.0 47 111688.5 48 111002.0 49 109697.0 50 106041.5 51 102785.0 52 97187.0 53 93114.0 54 91049.5 55 85939.5 56 78506.5 57 71743.5 58 64545.5 59 56956.0 60 50216.5 61 42774.0 62 36325.5 63 30207.0 64 24506.5 65 19867.0 66 15358.0 67 11607.5 68 9217.5 69 7506.0 70 5715.5 71 4187.0 72 3185.0 73 2366.5 74 1756.0 75 1151.5 76 660.5 77 426.0 78 282.5 79 197.0 80 132.0 81 74.5 82 52.0 83 39.5 84 28.0 85 17.5 86 16.0 87 12.0 88 6.5 89 9.0 90 10.5 91 8.0 92 5.5 93 5.5 94 5.5 95 5.5 96 5.5 97 3.0 98 2.0 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.331949157693282 2 0.12038903367591655 3 0.06519272889728643 4 0.0374865904449612 5 0.04180603296536826 6 0.010366662048976925 7 0.007034520676091485 8 0.01644473473840685 9 0.01838848387259002 10-11 0.021628065762895308 12-13 0.016583573962277075 14-15 0.01957633056570196 16-17 0.020393939328493295 18-19 0.02013168746118287 20-21 0.025052766618360898 22-23 0.016167056290666396 24-25 0.02599378802459244 26-27 0.019468344502691783 28-29 0.019221519215811382 30-31 0.020424792489353347 32-33 0.026055494346312536 34-35 0.01541115384959516 36-37 0.009857584894786093 38-39 0.014084467932612994 40-41 0.0103512354685469 42-43 0.009055402712424783 44-45 0.009271374838445137 46-47 0.008222367369203424 48-49 0.004118896974816724 50-51 0.006340324556740352 52-53 0.007466464928132189 54-55 0.0032704350511653392 56-57 0.002637945253534307 58-59 0.002730504736114458 60-61 0.003625246401055919 62-63 0.0026842249948243824 64-65 0.0021288680993434756 66-67 0.0016197909451526444 68-69 0.0028847705404147096 70-71 0.0019283225537531483 72-73 0.00166607068644272 74-75 0.001990028875473249 76-77 0.003625246401055919 78-79 0.0032087287294452387 80-81 0.0032395818903052888 82-83 0.001990028875473249 84-85 0.0027922110578345585 86-87 0.002190574421063576 88-89 0.6018063291557125 90-91 0.6123735367502797 92-93 0.6092727940838447 94-95 0.5964378791660637 96-97 0.5947718084796211 98-99 0.5921492898065168 100 0.5939541997168297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3241159.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.107964012759396 #Duplication Level Percentage of deduplicated Percentage of total 1 75.27425381112496 45.99856392977541 2 14.168136042339349 17.315718948063047 3 4.8315390978484976 8.85736551952698 4 2.1218873426309584 5.186568614904892 5 1.102560990935151 3.3687628677968777 6 0.6661711450013361 2.4425017413048224 7 0.41036728301040265 1.7553696413549473 8 0.26722670056931275 1.3063743681310396 9 0.20355667155850274 1.1195040384138661 >10 0.8961516406473812 9.37588185902995 >50 0.04375743771530235 1.800054894921256 >100 0.01403821990920129 1.3239550494014274 >500 3.0310003747567355E-4 0.10545093027279598 >1k 5.051667215405376E-5 0.04392759710280783 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.085316086005037E-5 0.0 5 0.0 0.0 0.0 3.085316086005037E-5 0.0 6 0.0 0.0 0.0 3.085316086005037E-5 0.0 7 0.0 0.0 0.0 3.085316086005037E-5 0.0 8 0.0 0.0 0.0 3.085316086005037E-5 0.0 9 0.0 0.0 0.0 3.085316086005037E-5 0.0 10-11 0.0 0.0 0.0 1.0798606301017629E-4 0.0 12-13 0.0 0.0 0.0 1.6969238473027703E-4 0.0 14-15 0.0 0.0 0.0 2.1597212602035259E-4 0.0 16-17 0.0 0.0 0.0 2.4682528688040295E-4 0.0 18-19 0.0 0.0 0.0 2.4682528688040295E-4 0.0 20-21 0.0 0.0 0.0 3.8566451075062964E-4 0.0 22-23 0.0 0.0 0.0 4.319442520407052E-4 0.0 24-25 0.0 6.170632172010074E-5 0.0 5.553568954809067E-4 0.0 26-27 0.0 6.170632172010074E-5 0.0 7.713290215012593E-4 0.0 28-29 0.0 6.170632172010074E-5 0.0 0.0014809517212824178 0.0 30-31 0.0 6.170632172010074E-5 0.0 0.004905652576748009 0.0 32-33 0.0 6.170632172010074E-5 0.0 0.013559964197992137 0.0 34-35 0.0 6.170632172010074E-5 0.0 0.02534587164653138 0.0 36-37 0.0 9.255948258015111E-5 0.0 0.04686595134641651 0.0 38-39 0.0 1.2341264344020147E-4 0.0 0.08359663935030648 0.0 40-41 0.0 1.2341264344020147E-4 0.0 0.12626656081975615 0.0 42-43 0.0 1.2341264344020147E-4 0.0 0.16930672021952642 0.0 44-45 0.0 1.2341264344020147E-4 0.0 0.21939682687581818 0.0 46-47 0.0 1.2341264344020147E-4 0.0 0.28124198781978915 0.0 48-49 0.0 1.2341264344020147E-4 0.0 0.35050733395060224 0.0 50-51 0.0 1.2341264344020147E-4 0.0 0.4240766960213923 0.0 52-53 0.0 1.2341264344020147E-4 0.0 0.500469122310877 0.0 54-55 0.0 1.2341264344020147E-4 0.0 0.5809650189947484 0.0 56-57 0.0 1.2341264344020147E-4 0.0 0.6798956792924999 0.0 58-59 0.0 1.2341264344020147E-4 0.0 0.7897175053738492 0.0 60-61 0.0 1.2341264344020147E-4 0.0 0.9082553493981629 0.0 62-63 0.0 1.2341264344020147E-4 0.0 1.0316217130970742 0.0 64-65 0.0 1.2341264344020147E-4 0.0 1.1606496318138049 0.0 66-67 0.0 1.5426580430025186E-4 0.0 1.3081894470465656 0.0 68-69 0.0 1.5426580430025186E-4 0.0 1.4766939850837308 0.0 70-71 0.0 1.5426580430025186E-4 0.0 1.6566296192195447 0.0 72-73 0.0 1.5426580430025186E-4 0.0 1.8409618287779155 0.0 74-75 0.0 1.5426580430025186E-4 0.0 2.0277005848833705 0.0 76-77 0.0 1.5426580430025186E-4 0.0 2.235265224569359 0.0 78-79 0.0 1.5426580430025186E-4 0.0 2.4704125900642335 0.0 80-81 0.0 1.5426580430025186E-4 0.0 2.7295482881277966 0.0 82-83 0.0 1.5426580430025186E-4 0.0 2.9922320996902654 0.0 84-85 0.0 1.5426580430025186E-4 0.0 3.2608705712987236 0.0 86-87 0.0 2.1597212602035259E-4 0.0 3.544503679085167 0.0 88 0.0 2.4682528688040295E-4 0.0 3.7777844283480078 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8735 0.0 27.503862 1 TACATGG 8960 0.0 25.654325 2 ACATGGG 9040 0.0 24.947748 3 GTATCAA 5235 0.0 24.745852 1 TCAACGC 5115 0.0 23.876959 4 CAACGCA 5155 0.0 23.69132 5 ATCAACG 5240 0.0 23.671793 3 AACGCAG 5430 0.0 22.658197 6 GGTATCA 3820 0.0 20.840576 1 TATCAAC 6175 0.0 20.553534 2 CATGGGG 6525 0.0 20.085144 4 CCTATAC 1050 0.0 19.688921 3 TACGTCG 245 1.9777872E-7 19.17249 5 GAGTACA 5965 0.0 18.87443 1 ACGCAGA 6240 0.0 18.813921 7 ACGTCGC 225 1.8627343E-6 18.78382 6 CGCAGAG 6290 0.0 18.664368 8 CCGTCGT 155 0.0010398699 18.17789 9 GTATAGG 1115 0.0 17.74438 1 AGTACAT 6365 0.0 16.764368 2 >>END_MODULE