FastQCFastQC Report
Wed 25 May 2016
SRR1033140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241159
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41520.12810232389092915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32730.10098239549494487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89400.032.955061
GGTATCA60000.032.0032581
TCAACGC98450.029.4662954
ATCAACG98900.029.284683
CAACGCA101050.028.7546585
AACGCAG103650.028.0678986
TATACCG2350.028.0102025
TATCAAC106450.027.295992
GTACATG86000.027.0342161
TACATGG87900.025.3539452
ACATGGG87300.024.6664873
ACGCAGA117050.024.653897
CGCAGAG118500.024.2306168
GCAGAGT132150.021.229949
GAGTACT71650.019.77452512-13
AGAGTAC104350.019.70246110-11
CCGATCG1751.07955915E-418.7975659
CATGGGG61050.018.7144344
GTATTAG14850.017.7478961
GTACTTT81000.017.43389314-15
Produced by FastQC (version 0.11.5)