FastQCFastQC Report
Wed 25 May 2016
SRR1033139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3900426
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53900.030.2422331
CAACGCA51950.029.6614975
TCAACGC52000.029.6333584
ATCAACG52450.029.4766273
AACGCAG57700.026.8581356
GTACATG89400.025.6636221
TACATGG92150.024.129862
TATCAAC64700.023.8318942
GGTATCA36350.023.3289171
ACGCAGA66750.022.8649257
CGCAGAG66350.022.8612168
ACATGGG94700.022.2804373
GCAGAGT82250.017.4141249
CATGGGG60200.017.1686674
TCTGTCG9250.016.7536688
GAGTACA67000.016.7351131
AGAGTAC61650.016.52967610-11
GTCTTAC15950.015.9499591
CTGTCGC9750.015.8945069
AGTACAT68200.014.4751792
Produced by FastQC (version 0.11.5)