FastQCFastQC Report
Wed 25 May 2016
SRR1033139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3900426
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52240.1339340882252349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.10901373337168811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62150.042.478371
GTATCAA106950.037.9731031
TCAACGC120000.033.109594
ATCAACG121050.032.783553
CAACGCA122000.032.5672265
AACGCAG128200.031.1233546
TATCAAC131900.030.2654252
ACGCAGA145400.027.1507347
CGCAGAG146000.026.9093638
GTACATG90650.025.5932371
TACATGG90300.024.3692422
GCAGAGT166150.023.3064889
ACATGGG93800.022.7077273
GAGTACT105850.021.97651312-13
AGAGTAC140550.020.51321410-11
CAGAGTA163650.019.2832410-11
GTACTTT121300.019.06113414-15
CATGGGG58300.018.469084
AGTACTT117750.018.2189912-13
CCTATAC13500.017.0663493
Produced by FastQC (version 0.11.5)