FastQCFastQC Report
Wed 25 May 2016
SRR1033138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749271
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40950.032.443521
TCAACGC40450.030.5485524
ATCAACG40700.030.3642223
CAACGCA41550.029.7381785
GGTATCA25150.027.911361
AACGCAG44500.027.7546486
GTACATG82400.026.5863341
TACATGG82150.025.355132
TATCAAC50000.025.2960052
TAGACCG1151.3815159E-424.5122435
ACATGGG84750.023.5086123
ACGCAGA52600.023.1235247
CGCAGAG53550.022.538328
GTACCGA1303.1788487E-421.6744426
ACCGCGT1554.2676664E-521.208718
GAGTACA60100.019.832561
ATAACCG1550.001036513718.1865025
GCAGAGT63750.018.1218629
CATGGGG53500.017.6520714
TCTACGC1902.0180494E-417.322642
Produced by FastQC (version 0.11.5)