##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033138_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2749271
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12202362007965	33.0	31.0	34.0	28.0	34.0
2	31.302750801939858	33.0	31.0	34.0	28.0	34.0
3	31.42111745259016	34.0	31.0	34.0	28.0	34.0
4	34.798648441714185	37.0	35.0	37.0	32.0	37.0
5	34.857338545381666	37.0	35.0	37.0	32.0	37.0
6	34.97035104942365	37.0	35.0	37.0	32.0	37.0
7	34.902238811670436	37.0	35.0	37.0	32.0	37.0
8	34.90668035271896	37.0	35.0	37.0	32.0	37.0
9	36.509323744367144	39.0	38.0	39.0	32.0	39.0
10-11	36.48946684411977	39.0	37.0	39.0	32.0	39.0
12-13	36.41968088995228	39.0	37.0	39.0	32.0	39.0
14-15	37.796354742766354	41.0	38.0	41.0	32.5	41.0
16-17	37.78867125139719	40.5	38.0	41.0	32.0	41.0
18-19	37.76697713684828	40.0	38.0	41.0	32.0	41.0
20-21	37.78209114343402	40.0	38.0	41.0	32.0	41.0
22-23	37.73110853749958	40.0	38.0	41.0	32.0	41.0
24-25	37.712426494150634	40.0	38.0	41.0	32.0	41.0
26-27	37.6586231404616	40.0	38.0	41.0	32.0	41.0
28-29	37.624168006718875	40.0	38.0	41.0	32.0	41.0
30-31	37.61667074653609	40.0	38.0	41.0	32.0	41.0
32-33	37.6833802488005	40.0	38.0	41.0	32.0	41.0
34-35	37.65329609194583	40.0	38.0	41.0	32.0	41.0
36-37	37.57065418432741	40.0	38.0	41.0	31.0	41.0
38-39	37.48430674895272	40.0	38.0	41.0	31.0	41.0
40-41	37.41781403142869	40.0	38.0	41.0	31.0	41.0
42-43	37.29760361928671	40.0	38.0	41.0	31.0	41.0
44-45	37.16680185401876	40.0	38.0	41.0	30.0	41.0
46-47	37.03427253988421	40.0	37.0	41.0	30.0	41.0
48-49	36.906776196308044	40.0	37.0	41.0	30.0	41.0
50-51	36.7734681302789	40.0	37.0	41.0	30.0	41.0
52-53	36.617523699919	40.0	36.5	41.0	30.0	41.0
54-55	36.45742234941554	40.0	36.0	41.0	30.0	41.0
56-57	36.293493620672535	40.0	35.5	41.0	30.0	41.0
58-59	36.08449839975761	39.0	35.0	41.0	29.0	41.0
60-61	35.85546386660319	39.0	35.0	41.0	29.0	41.0
62-63	35.58793240098921	39.0	35.0	41.0	28.5	41.0
64-65	35.318526620329536	38.0	35.0	40.5	28.0	41.0
66-67	35.02088790082898	37.5	34.5	40.0	28.0	41.0
68-69	34.708727331718116	37.0	34.0	40.0	28.0	41.0
70-71	34.356761119584064	36.5	34.0	39.5	27.5	41.0
72-73	33.98846857948889	36.0	34.0	39.0	26.5	41.0
74-75	33.61736020203174	36.0	34.0	39.0	26.0	40.5
76-77	33.289236674012855	35.0	34.0	37.5	26.0	40.0
78-79	32.920930493938215	35.0	33.0	37.0	26.0	39.0
80-81	32.56100180738821	35.0	33.0	37.0	26.0	39.0
82-83	32.33836169660975	35.0	33.0	36.0	26.0	38.5
84-85	32.066860633236956	35.0	33.0	36.0	25.0	37.0
86-87	31.80947131075838	35.0	33.0	36.0	25.0	37.0
88-89	31.441790751075466	35.0	33.0	35.0	23.5	36.5
90-91	31.246866169249955	35.0	33.0	35.0	23.0	36.0
92-93	31.06026761275989	35.0	33.0	35.0	20.5	36.0
94-95	30.88074984241277	35.0	33.0	35.0	20.0	36.0
96-97	30.693894308709474	35.0	32.0	35.0	18.0	35.5
98-99	30.48443441916057	35.0	32.0	35.0	7.5	35.0
100	30.30828354134605	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30064654214278974
1101	2	0.3188121972888496
1101	3	0.31029760745328616
1101	4	0.22562133603110368
1101	5	0.2760950706894221
1101	6	0.31265291486465685
1101	7	0.37935560573064464
1101	8	0.34819827135417825
1101	9	0.4365231665626652
1101	10-11	0.42314273060475216
1101	12-13	0.4154768259544852
1101	14-15	0.4846344903390687
1101	16-17	0.3702012479830401
1101	18-19	0.3862992978381996
1101	20-21	0.33884506404515236
1101	22-23	0.3044315531388335
1101	24-25	0.24621970151426353
1101	26-27	0.23257351545571936
1101	28-29	0.2996810691935323
1101	30-31	0.3574741802798016
1101	32-33	0.47221437898868146
1101	34-35	0.4118217445322401
1101	36-37	0.3533795348227855
1101	38-39	0.4187567872970135
1101	40-41	0.4447344358262839
1101	42-43	0.41236001879790507
1101	44-45	0.3653360071075369
1101	46-47	0.3602029413405816
1101	48-49	0.3097768320586667
1101	50-51	0.3253704307048437
1101	52-53	0.3358497955260731
1101	54-55	0.3403759428825026
1101	56-57	0.3734454991269658
1101	58-59	0.30824004804443916
1101	60-61	0.38761169234711446
1101	62-63	0.3418054923529539
1101	64-65	0.3162498284306281
1101	66-67	0.3478245017471764
1101	68-69	0.32254126360862756
1101	70-71	0.28111976578784237
1101	72-73	0.33417349688004805
1101	74-75	0.30619597767172735
1101	76-77	0.289741336542491
1101	78-79	0.3240547700102212
1101	80-81	0.37191641119681407
1101	82-83	0.36421983541793423
1101	84-85	0.28166049862153386
1101	86-87	0.36557326950230973
1101	88-89	0.5505792084517651
1101	90-91	0.6112454677208632
1101	92-93	0.6625734099073455
1101	94-95	0.6754658498197301
1101	96-97	0.6350709445794216
1101	98-99	0.6877596026274375
1101	100	0.6579530740000266
1102	1	0.2445919803011698
1102	2	0.25370527161136636
1102	3	0.23037398548177634
1102	4	0.17199954264364692
1102	5	0.060600163151725894
1102	6	0.10260649668782662
1102	7	0.12904834803673282
1102	8	0.1442477033282188
1102	9	0.2097379192068587
1102	10-11	0.2045411538829498
1102	12-13	0.17338066943652564
1102	14-15	0.21867012866915303
1102	16-17	0.14972787520197528
1102	18-19	0.20540171350709358
1102	20-21	0.12894352900352857
1102	22-23	0.20992663879693652
1102	24-25	0.20682010976622678
1102	26-27	0.2921659047271703
1102	28-29	0.2494349424216793
1102	30-31	0.2411848201239124
1102	32-33	0.21237686041962434
1102	34-35	0.2569842793993047
1102	36-37	0.24260203926688462
1102	38-39	0.2899910548124822
1102	40-41	0.2249486434220529
1102	42-43	0.2689166308053501
1102	44-45	0.20132872554548698
1102	46-47	0.27288105283682995
1102	48-49	0.2440041635656769
1102	50-51	0.2538720106328114
1102	52-53	0.16007463334744898
1102	54-55	0.19993684046683313
1102	56-57	0.16322249238333342
1102	58-59	0.22967003425792143
1102	60-61	0.2059646486114346
1102	62-63	0.3063157146828388
1102	64-65	0.3393365481296726
1102	66-67	0.3026813463798703
1102	68-69	0.24920920086749732
1102	70-71	0.2602131329403008
1102	72-73	0.2727655263482873
1102	74-75	0.1707594580018963
1102	76-77	0.1254247667139481
1102	78-79	0.19067290480461452
1102	80-81	0.2040137789335148
1102	82-83	0.14354525783047478
1102	84-85	0.23440533402047237
1102	86-87	0.278861443376325
1102	88-89	0.3876962228688292
1102	90-91	0.3504138235626755
1102	92-93	0.3852435059854926
1102	94-95	0.36668362338595273
1102	96-97	0.3644548617320602
1102	98-99	0.47312059924671246
1102	100	0.4498255112927403
1103	1	0.23269314347580305
1103	2	0.2648928936129735
1103	3	0.27758070688271985
1103	4	0.31306595428668516
1103	5	0.24127261119037513
1103	6	0.31589991129568773
1103	7	0.2849640465317407
1103	8	0.3185638356827454
1103	9	0.3915894523425365
1103	10-11	0.37900523643943984
1103	12-13	0.4568633501272146
1103	14-15	0.48036810017060105
1103	16-17	0.44754239341432367
1103	18-19	0.48652081786775625
1103	20-21	0.4734384231134072
1103	22-23	0.4399050655771575
1103	24-25	0.36674606623314787
1103	26-27	0.4801774902818394
1103	28-29	0.5057187371869674
1103	30-31	0.4889700665051393
1103	32-33	0.4284582672467536
1103	34-35	0.43331648987591365
1103	36-37	0.4292549738919931
1103	38-39	0.47365049561957306
1103	40-41	0.5058126329108816
1103	42-43	0.42392457748306356
1103	44-45	0.34870081311805023
1103	46-47	0.4290598043273661
1103	48-49	0.4551712901530891
1103	50-51	0.4076389411622543
1103	52-53	0.40544131820564644
1103	54-55	0.4693914537313759
1103	56-57	0.3798332899423542
1103	58-59	0.3953358358287744
1103	60-61	0.3879978689747716
1103	62-63	0.35006298809131664
1103	64-65	0.42451466833408347
1103	66-67	0.3656944117787191
1103	68-69	0.385421039278782
1103	70-71	0.371419436905434
1103	72-73	0.5030735563420805
1103	74-75	0.38128311754535105
1103	76-77	0.3709722491825005
1103	78-79	0.33353357191494837
1103	80-81	0.36601620485148345
1103	82-83	0.3682188965876776
1103	84-85	0.316646690618434
1103	86-87	0.391940802372833
1103	88-89	0.4934805830543496
1103	90-91	0.5600739726420088
1103	92-93	0.4764806043959844
1103	94-95	0.4709994649040503
1103	96-97	0.5014136365237256
1103	98-99	0.603314210767941
1103	100	0.5689680570695401
1104	1	0.12267166697637322
1104	2	0.1392504677474271
1104	3	0.14533008237667033
1104	4	0.16954729653314615
1104	5	0.03807841142366186
1104	6	0.04212088987914342
1104	7	0.07439302029959549
1104	8	0.05242049156255035
1104	9	0.053462647653709894
1104	10-11	0.05745790866667022
1104	12-13	0.07306954270949717
1104	14-15	0.08204766206898029
1104	16-17	0.12347190336389957
1104	18-19	0.09086771253794268
1104	20-21	0.07279784363051078
1104	22-23	0.11381878686022162
1104	24-25	0.03663535537550189
1104	26-27	0.051475044865753716
1104	28-29	0.1675004415582677
1104	30-31	0.22597677785437043
1104	32-33	0.1525776174214002
1104	34-35	0.12352826493201263
1104	36-37	0.18881298398710555
1104	38-39	0.0791197700908981
1104	40-41	0.0699960767597716
1104	42-43	0.061005579345554395
1104	44-45	0.07561740865215683
1104	46-47	-0.0228539932143903
1104	48-49	-0.024619994881824425
1104	50-51	-0.05101436416799032
1104	52-53	-0.0040840805821460435
1104	54-55	-0.022530159351660473
1104	56-57	0.003468176590693872
1104	58-59	-0.0569204437722135
1104	60-61	-0.02422503478332061
1104	62-63	-0.025677018366636162
1104	64-65	0.012897936710032809
1104	66-67	0.02230764128417917
1104	68-69	-0.10550813906893097
1104	70-71	-0.031689579177559324
1104	72-73	0.05417928459729637
1104	74-75	0.05026529204432961
1104	76-77	-0.08248192191121007
1104	78-79	-0.12194298090909683
1104	80-81	-0.1315733969969628
1104	82-83	-0.17231061542751291
1104	84-85	-0.14036948876018585
1104	86-87	-0.06767148520140509
1104	88-89	0.1202946905480502
1104	90-91	0.026803261506351816
1104	92-93	-0.041035412364557544
1104	94-95	-0.02836672405887697
1104	96-97	-0.013211987551986937
1104	98-99	0.04408293732643642
1104	100	0.16939155911407155
1105	1	0.19774017721241677
1105	2	0.25061059464008295
1105	3	0.19417080720047153
1105	4	0.266375867476107
1105	5	0.21605643216018677
1105	6	0.21626579915119493
1105	7	0.11027522518444499
1105	8	0.19500995103403795
1105	9	0.23426749670873903
1105	10-11	0.23732949009086468
1105	12-13	0.30673290700449485
1105	14-15	0.2932750488171365
1105	16-17	0.32454397858169415
1105	18-19	0.3111042203205159
1105	20-21	0.4011019690914779
1105	22-23	0.40727348487279613
1105	24-25	0.34229701653752187
1105	26-27	0.336313568413928
1105	28-29	0.41204280517891334
1105	30-31	0.4228422595930539
1105	32-33	0.4904152196681082
1105	34-35	0.3471876288390874
1105	36-37	0.3836796464713501
1105	38-39	0.39477246568500135
1105	40-41	0.2893833925432219
1105	42-43	0.278918869031628
1105	44-45	0.32512982622617415
1105	46-47	0.420364430715253
1105	48-49	0.39293883369062144
1105	50-51	0.32174786807096467
1105	52-53	0.3109436246973658
1105	54-55	0.21719952265186748
1105	56-57	0.21339437499494096
1105	58-59	0.27526181470108213
1105	60-61	0.2511777409185143
1105	62-63	0.2843824138257318
1105	64-65	0.26597308651525253
1105	66-67	0.3168475741786452
1105	68-69	0.21886711431396577
1105	70-71	0.2991615286587077
1105	72-73	0.25232067887682774
1105	74-75	0.31870210016217726
1105	76-77	0.2887198965969091
1105	78-79	0.2893591647285092
1105	80-81	0.3606889267222826
1105	82-83	0.3133899973404297
1105	84-85	0.28224774972492384
1105	86-87	0.330070645119509
1105	88-89	0.3722597358307915
1105	90-91	0.37623808330225117
1105	92-93	0.3563531066595935
1105	94-95	0.38173355558086186
1105	96-97	0.4324678434444351
1105	98-99	0.49857565039676643
1105	100	0.48948339182412326
1106	1	0.1578943218224751
1106	2	0.1227822127512539
1106	3	0.09882339002646745
1106	4	0.10118957745390134
1106	5	0.0758204641636624
1106	6	0.12305017673809004
1106	7	0.07694085193186595
1106	8	0.17083671064884243
1106	9	0.12245645114693815
1106	10-11	0.11186634895684477
1106	12-13	0.09125451477998325
1106	14-15	0.1083470628279457
1106	16-17	0.11270343821107787
1106	18-19	0.11950316779523007
1106	20-21	0.06811522258441727
1106	22-23	0.10772613552375532
1106	24-25	0.13987862082649372
1106	26-27	0.16194346178966157
1106	28-29	0.2402816852668579
1106	30-31	0.21637722850503138
1106	32-33	0.24902485795720963
1106	34-35	0.19989279220484235
1106	36-37	0.1753775305611498
1106	38-39	0.176968305976267
1106	40-41	0.12536909547941377
1106	42-43	0.19566372809954657
1106	44-45	0.22709453533614976
1106	46-47	0.22517154871639633
1106	48-49	0.17756921622530797
1106	50-51	0.15532915466857844
1106	52-53	0.039752551205396
1106	54-55	0.10061530729237234
1106	56-57	0.07187990170054803
1106	58-59	0.12800180742323874
1106	60-61	0.13192392981294176
1106	62-63	0.13126613307230173
1106	64-65	0.05060989908497504
1106	66-67	0.13080885917589313
1106	68-69	0.05717751306187324
1106	70-71	0.13153176578887127
1106	72-73	0.16227107472883517
1106	74-75	0.15306159961014743
1106	76-77	0.13177286293876733
1106	78-79	0.06503887896823102
1106	80-81	0.06593665691991646
1106	82-83	0.10167258023310666
1106	84-85	0.03510899623721997
1106	86-87	0.08078717726349183
1106	88-89	0.2449811814741345
1106	90-91	0.209205301554789
1106	92-93	0.17853816830695735
1106	94-95	0.17732899349482523
1106	96-97	0.14462875435653544
1106	98-99	0.13466954462602843
1106	100	0.18755240467561762
1107	1	0.03882326233075872
1107	2	0.008089026626823426
1107	3	0.10367169165010282
1107	4	0.0045116964577403
1107	5	-0.04939427499973448
1107	6	-0.0036800215479857457
1107	7	0.0013091376312175385
1107	8	-0.030108759461448642
1107	9	0.01585576459373783
1107	10-11	-0.038710537780694665
1107	12-13	-0.041060772131949363
1107	14-15	-0.042905143508910726
1107	16-17	-0.1269269627445624
1107	18-19	-0.021508013774322876
1107	20-21	-0.029457917390438126
1107	22-23	-0.05434320016012606
1107	24-25	0.01102809470067001
1107	26-27	0.048007240072166724
1107	28-29	0.02813857736387604
1107	30-31	0.05952491384497449
1107	32-33	0.0684408263779801
1107	34-35	0.05489813184895098
1107	36-37	9.638733207140149E-5
1107	38-39	-0.05015499462999884
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2216	30-31	-0.34243431260247803
2216	32-33	-0.3053016159746704
2216	34-35	-0.2813461747852344
2216	36-37	-0.29917440028378905
2216	38-39	-0.26244308288008256
2216	40-41	-0.25582542545553366
2216	42-43	-0.29072643063577175
2216	44-45	-0.18512738854280286
2216	46-47	-0.13876478624768396
2216	48-49	-0.21703144775777616
2216	50-51	-0.19663485236618783
2216	52-53	-0.17988071375836512
2216	54-55	-0.21234808427212215
2216	56-57	-0.2804307063177802
2216	58-59	-0.35013262784006116
2216	60-61	-0.2206131296722944
2216	62-63	-0.20699079086533345
2216	64-65	-0.20815432349823482
2216	66-67	-0.18942821116089448
2216	68-69	-0.1340439431914433
2216	70-71	-0.2455277379761256
2216	72-73	-0.20469298687255133
2216	74-75	-0.15480681412177688
2216	76-77	-0.22587122021745643
2216	78-79	-0.22742512096257883
2216	80-81	-0.2100981578181802
2216	82-83	-0.26024036274230156
2216	84-85	-0.32244500052048863
2216	86-87	-0.24361620883198754
2216	88-89	-0.23579674906729764
2216	90-91	-0.2289656748108655
2216	92-93	-0.18617764565505368
2216	94-95	-0.2787467832712842
2216	96-97	-0.32091623540861747
2216	98-99	-0.29585175807889286
2216	100	-0.2428526093582981
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47208.0
3	8025.0
4	5048.0
5	3928.0
6	3278.0
7	3473.0
8	4038.0
9	4574.0
10	4904.0
11	5230.0
12	5891.0
13	6364.0
14	6665.0
15	7042.0
16	7116.0
17	7083.0
18	7151.0
19	7100.0
20	7427.0
21	7474.0
22	8614.0
23	11645.0
24	16972.0
25	21908.0
26	22512.0
27	20634.0
28	22636.0
29	27644.0
30	33615.0
31	41428.0
32	53003.0
33	71266.0
34	98000.0
35	149081.0
36	273352.0
37	555477.0
38	838623.0
39	323303.0
40	539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11224052223464	19.63113364580699	13.437462021969504	23.819163809988865
2	15.25298304584777	19.818386087070525	40.865206204808196	24.063424662273505
3	18.455891318392343	25.077971592098198	29.565473753173364	26.900663336336088
4	12.078588824518945	16.399089287189287	38.883266980256366	32.6390549080354
5	12.837997161675338	38.21578545176668	35.679524034787676	13.266693351770314
6	31.30268493314131	37.89113669044858	17.745425893786372	13.060752482623725
7	27.759745515326006	32.44374037179644	22.163883619458836	17.63263049341872
8	25.11881323548885	36.97554470011481	20.078963872918585	17.82667819147775
9	24.938063296582403	17.267007036591586	20.90888519754625	36.88604446927976
10-11	24.001648782722125	27.034997887178804	28.711812098434365	20.251541231664703
12-13	24.470608222345547	24.322927665630413	28.80229231364447	22.404171798379572
14-15	21.73644859847084	26.43810466916876	25.739936709770262	26.085510022590142
16-17	21.63051412707574	29.782575439386644	26.37400356853569	22.21290686500192
18-19	21.53989211827481	28.784818995973083	28.212177094251174	21.463111791500943
20-21	22.628702279078414	28.08454888669222	27.73855594698559	21.548192887243776
22-23	22.26795268979306	27.792763918982434	27.827233054281887	22.11205033694262
24-25	22.1360769021011	27.94464643723108	27.66768579693082	22.251590863737004
26-27	21.918110226480835	27.87800481785871	27.831710736388764	22.37217421927169
28-29	21.93418123080953	27.893504707577232	27.883718476694945	22.28859558491829
30-31	22.121456806458674	28.348604277926036	27.52884641445521	22.001092501160077
32-33	21.822583409038735	28.0759283022259	28.01076769096043	22.090720597774936
34-35	21.977727150776	27.816396782305407	28.07319391202725	22.13268215489134
36-37	22.256343658887488	28.041273115181674	27.607643492827407	22.094739733103435
38-39	22.070956893109937	28.242284022194152	27.71338588119527	21.973373203500646
40-41	21.814848395835966	27.99309207744221	28.03728968622426	22.15476984049756
42-43	21.852040873860467	27.878428966007444	27.986030047547455	22.283500112584633
44-45	21.924067973930388	28.06873856526197	27.88794716089289	22.119246299914753
46-47	22.10297490204338	28.15385032312089	27.76902626490584	21.97414850992989
48-49	22.055190310285898	28.03405571629257	27.704862098793004	22.20589187462852
50-51	21.909993423273463	27.99353822067537	27.801892845918356	22.294575510132812
52-53	21.975192642876383	27.927965287087765	27.883295529550335	22.213546540485517
54-55	22.07144575839347	28.302311619497694	27.681289287901308	21.944953334207533
56-57	21.954903080543726	28.053353896966016	27.68664588478788	22.30509713770238
58-59	21.865087967920662	27.662826496880527	28.084678944734147	22.38740659046466
60-61	22.098059246727505	28.018457971503707	27.72431405978071	22.15916872198808
62-63	21.86196771913692	28.074868060802068	27.81253557875194	22.250628641309074
64-65	21.87008437886191	27.881671354381215	27.868994974006146	22.379249292750732
66-67	21.79570000421939	27.923837812434982	27.751297462691127	22.529164720654503
68-69	22.142586543328363	28.04551455494198	27.64819702119154	22.163701880538113
70-71	22.100657808228597	27.773696800766622	27.75196324901867	22.373682141986112
72-73	21.893803639106896	27.866464597120967	27.923335304196446	22.316396459575692
74-75	21.89514442330706	27.917456287442576	27.853801637561336	22.33359765168903
76-77	21.929703472158945	27.9105664130809	27.73875082593155	22.420979288828605
78-79	22.004506798390793	28.04278002895409	27.74050807877258	22.212205093882538
80-81	21.91779302156778	27.82050076559457	27.92595075496409	22.335755457873567
82-83	21.817877267495184	27.86029350650815	28.00204349873318	22.319785727263486
84-85	21.815210689713496	27.81318827216066	27.86162080762117	22.509980230504677
86-87	22.004171386211148	27.834127380778305	27.773473504713724	22.388227728296826
88-89	21.763174063893924	27.825591016397066	27.866705359648954	22.54452956006006
90-91	21.785666119581307	27.708205200745073	27.95593989588116	22.550188783792464
92-93	21.89596541744557	27.8726144346739	27.850290359366376	22.381129788514155
94-95	21.670367645619358	27.655506524267558	28.031581117853598	22.642544712259483
96-97	21.753836133477503	27.621441731204456	27.961374293560493	22.66334784175755
98-99	21.68483856780196	27.70180030984561	27.919182546193266	22.694178576159157
100	21.762512139092966	27.60490115649788	27.815958543762576	22.81662816064658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	305.0
1	270.5
2	227.0
3	209.5
4	172.0
5	128.5
6	128.0
7	142.0
8	141.5
9	145.0
10	151.5
11	157.5
12	158.5
13	172.0
14	198.0
15	256.5
16	371.0
17	480.0
18	621.0
19	961.5
20	1501.5
21	2309.0
22	3504.5
23	5332.0
24	7708.0
25	10506.5
26	14561.0
27	19844.0
28	26516.5
29	33834.5
30	40698.5
31	48349.5
32	56553.5
33	64874.0
34	72565.0
35	79229.0
36	85806.5
37	92023.0
38	96768.5
39	99188.0
40	101574.5
41	102930.0
42	101876.0
43	101798.5
44	101025.5
45	98875.5
46	97620.5
47	95508.0
48	92361.5
49	90115.5
50	87777.0
51	84065.5
52	78696.0
53	74121.5
54	70799.0
55	67115.0
56	61955.5
57	55500.0
58	49960.0
59	45561.5
60	41143.0
61	35696.0
62	30698.0
63	25902.5
64	20934.5
65	17009.0
66	13467.5
67	10105.0
68	7725.5
69	5885.0
70	4178.0
71	2933.5
72	2082.0
73	1483.5
74	1174.0
75	894.5
76	587.5
77	418.5
78	290.5
79	167.5
80	104.5
81	59.0
82	35.5
83	24.5
84	21.0
85	15.0
86	10.5
87	10.5
88	12.5
89	10.5
90	5.0
91	4.0
92	3.0
93	1.5
94	2.0
95	2.5
96	4.0
97	3.0
98	2.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3346341630199424
2	0.12134125737331823
3	0.06532640834606701
4	0.039792366776501845
5	0.042593109227864405
6	0.00960254554752878
7	0.006765429817577095
8	0.015240403728843029
9	0.018077519458794714
10-11	0.020969195106630086
12-13	0.015731442989796205
14-15	0.01838669232680227
16-17	0.019077784619995628
18-19	0.018914104866344567
20-21	0.02400636386882195
22-23	0.015822376186269017
24-25	0.02493388247284462
26-27	0.01962338379883249
28-29	0.01858674535904245
30-31	0.01936877084870862
32-33	0.024661082883426187
34-35	0.014858484303657224
36-37	0.00967529210470703
38-39	0.012676087588309773
40-41	0.009220626122342977
42-43	0.008293107518320311
44-45	0.008966013172219109
46-47	0.008093054486080128
48-49	0.0046921529379970175
50-51	0.006656309981809724
52-53	0.007547455307243265
54-55	0.0034372748266722343
56-57	0.0029462355657190577
58-59	0.003164475237253803
60-61	0.0038737541697417242
62-63	0.003200848515842927
64-65	0.0023097031904093846
66-67	0.002146023436758326
68-69	0.0030735420407809926
70-71	0.002382449747587633
72-73	0.001909597125929019
74-75	0.0022187699939365743
76-77	0.0037282610553852273
78-79	0.0033099683516102994
80-81	0.003655514498206979
82-83	0.0023460764689985088
84-85	0.0031281019586646783
86-87	0.002364263108293071
88-89	0.6055423419517392
90-91	0.6151630741385625
92-93	0.6108892139043405
94-95	0.5978130202515504
96-97	0.5956488101754974
98-99	0.5927753211669566
100	0.5952487041110172
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2749271.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.20457744934219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.19989592274781	47.39982327558381
2	13.910383057580459	17.30579000510562
3	4.556188408527286	8.502473241960923
4	1.9537583502457192	4.861308504606355
5	1.0209010588639427	3.175235949210879
6	0.5997120895530612	2.2382902273146144
7	0.37803014548251285	1.6460643823997145
8	0.25706499804096106	1.279249566412317
9	0.195044582862038	1.0919399274644654
>10	0.875220244174244	9.426437206434315
>50	0.0395384285064543	1.6524328055017963
>100	0.014145560397503337	1.3840439535996119
>500	1.171530179642334E-4	0.03691095440553199
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.6373278589124174E-5	0.0
4	0.0	0.0	0.0	7.274655717824835E-5	0.0
5	0.0	0.0	0.0	1.454931143564967E-4	0.0
6	0.0	0.0	0.0	1.454931143564967E-4	0.0
7	3.6373278589124174E-5	0.0	0.0	1.454931143564967E-4	0.0
8	3.6373278589124174E-5	0.0	0.0	1.454931143564967E-4	0.0
9	3.6373278589124174E-5	0.0	0.0	4.7285262165861424E-4	0.0
10-11	7.274655717824835E-5	0.0	0.0	4.7285262165861424E-4	0.0
12-13	7.274655717824835E-5	0.0	0.0	4.7285262165861424E-4	0.0
14-15	7.274655717824835E-5	1.8186639294562087E-5	0.0	4.7285262165861424E-4	0.0
16-17	1.0911983576737251E-4	3.6373278589124174E-5	0.0	5.092259002477384E-4	0.0
18-19	1.454931143564967E-4	5.455991788368626E-5	0.0	5.274125395423005E-4	0.0
20-21	1.6367975365105879E-4	7.274655717824835E-5	0.0	5.819724574259868E-4	0.0
22-23	2.0005303224018294E-4	7.274655717824835E-5	0.0	5.819724574259868E-4	0.0
24-25	2.1823967153474503E-4	7.274655717824835E-5	0.0	6.183457360151109E-4	0.0
26-27	2.727995894184313E-4	7.274655717824835E-5	0.0	6.729056538987972E-4	0.0
28-29	2.909862287129934E-4	7.274655717824835E-5	0.0	0.0014003712256812806	0.0
30-31	3.4554614659667963E-4	7.274655717824835E-5	0.0	0.004201113677043842	0.0
32-33	3.637327858912417E-4	7.274655717824835E-5	0.0	0.011239343084039368	0.0
34-35	3.637327858912417E-4	9.093319647281042E-5	0.0	0.0207691420743899	0.0
36-37	3.637327858912417E-4	1.273064750619346E-4	0.0	0.03859204858306074	0.0
38-39	4.001060644803659E-4	1.6367975365105879E-4	0.0	0.06845451030473168	0.0
40-41	4.001060644803659E-4	1.8186639294562085E-4	0.0	0.10597354716941326	0.0
42-43	4.001060644803659E-4	2.1823967153474503E-4	0.0	0.14321978444467642	0.0
44-45	4.3647934306949006E-4	2.1823967153474503E-4	0.0	0.1885227029274306	0.0
46-47	4.3647934306949006E-4	2.1823967153474503E-4	0.0	0.24171862286402468	0.0
48-49	4.546659823640521E-4	2.1823967153474503E-4	0.0	0.30117074671794813	0.0
50-51	4.7285262165861424E-4	2.1823967153474503E-4	0.0	0.3680975793219366	0.0
52-53	4.7285262165861424E-4	2.1823967153474503E-4	0.0	0.43427875971484803	0.0
54-55	4.7285262165861424E-4	2.1823967153474503E-4	0.0	0.505588572388826	0.0
56-57	4.910392609531764E-4	2.1823967153474503E-4	0.0	0.5919751090379959	0.0
58-59	5.274125395423005E-4	2.1823967153474503E-4	0.0	0.6912014130291266	0.0
60-61	5.455991788368625E-4	2.1823967153474503E-4	0.0	0.79924823707812	0.0
62-63	6.001590967205488E-4	2.1823967153474503E-4	0.0	0.9102049234142433	0.0
64-65	6.54719014604235E-4	2.1823967153474503E-4	0.0	1.0243260849876203	0.0
66-67	6.910922931933593E-4	2.1823967153474503E-4	0.0	1.1544333025009175	0.0
68-69	6.910922931933593E-4	2.1823967153474503E-4	0.0	1.3005265759541347	0.0
70-71	6.910922931933593E-4	2.1823967153474503E-4	0.0	1.4621330527256133	0.0
72-73	6.910922931933593E-4	2.1823967153474503E-4	0.0	1.6329419689801408	0.0
74-75	6.910922931933593E-4	2.1823967153474503E-4	0.0	1.8048420835923413	0.0
76-77	6.910922931933593E-4	2.546129501238692E-4	0.0	1.9865811700629004	0.0
78-79	7.274655717824834E-4	2.546129501238692E-4	0.0	2.199146610137742	0.0
80-81	7.638388503716076E-4	2.546129501238692E-4	0.0	2.4352273748204523	0.0
82-83	7.638388503716076E-4	2.546129501238692E-4	0.0	2.6829475886516825	0.0
84-85	7.638388503716076E-4	2.546129501238692E-4	0.0	2.9310860951866875	0.0
86-87	7.638388503716076E-4	2.546129501238692E-4	0.0	3.1889544537442838	0.0
88	7.638388503716076E-4	2.546129501238692E-4	0.0	3.4037750370916506	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4095	0.0	32.44352	1
TCAACGC	4045	0.0	30.548552	4
ATCAACG	4070	0.0	30.364222	3
CAACGCA	4155	0.0	29.738178	5
GGTATCA	2515	0.0	27.91136	1
AACGCAG	4450	0.0	27.754648	6
GTACATG	8240	0.0	26.586334	1
TACATGG	8215	0.0	25.35513	2
TATCAAC	5000	0.0	25.296005	2
TAGACCG	115	1.3815159E-4	24.512243	5
ACATGGG	8475	0.0	23.508612	3
ACGCAGA	5260	0.0	23.123524	7
CGCAGAG	5355	0.0	22.53832	8
GTACCGA	130	3.1788487E-4	21.674442	6
ACCGCGT	155	4.2676664E-5	21.20871	8
GAGTACA	6010	0.0	19.83256	1
ATAACCG	155	0.0010365137	18.186502	5
GCAGAGT	6375	0.0	18.121862	9
CATGGGG	5350	0.0	17.652071	4
TCTACGC	190	2.0180494E-4	17.32264	2
>>END_MODULE