FastQCFastQC Report
Wed 25 May 2016
SRR1033138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749271
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42330.15396808826776262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34860.12679724916168686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43000.046.952521
GTATCAA75050.040.195491
TCAACGC86700.034.4354364
ATCAACG87050.034.2969863
CAACGCA89000.033.5455325
AACGCAG92300.032.382396
TATCAAC94050.032.0948332
ACGCAGA108950.027.3042327
CGCAGAG109800.026.835088
GTACATG89400.025.6893041
TACATGG88500.024.597822
GCAGAGT120800.024.196989
ACATGGG91650.023.3415623
GAGTACT70750.021.45427112-13
CGCGCGT1554.2445834E-521.2236397
TAGCGCG1605.4016906E-520.5697524
CAGAGTA118400.020.22228210-11
AGAGTAC108050.019.63679910-11
GTACTTT78950.019.07715414-15
GAGTACA66800.018.8811451
Produced by FastQC (version 0.11.5)