##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033138_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2749271
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74433113359869	34.0	31.0	34.0	31.0	34.0
2	32.8219407981243	34.0	31.0	34.0	31.0	34.0
3	32.92739311621153	34.0	31.0	34.0	31.0	34.0
4	36.36267468721709	37.0	37.0	37.0	35.0	37.0
5	36.30142099487464	37.0	37.0	37.0	35.0	37.0
6	36.383810471939654	37.0	37.0	37.0	35.0	37.0
7	36.35152336746723	37.0	37.0	37.0	35.0	37.0
8	36.34316478804745	37.0	37.0	37.0	35.0	37.0
9	38.15716275332625	39.0	39.0	39.0	37.0	39.0
10-11	38.139724312372266	39.0	39.0	39.0	37.0	39.0
12-13	38.11422227928786	39.0	39.0	39.0	37.0	39.0
14-15	39.648725425758315	41.0	40.0	41.0	37.0	41.0
16-17	39.66009844064118	41.0	40.0	41.0	37.0	41.0
18-19	39.66921031066053	41.0	40.0	41.0	37.0	41.0
20-21	39.66352025682444	41.0	40.0	41.0	37.0	41.0
22-23	39.601235745766786	41.0	40.0	41.0	37.0	41.0
24-25	39.5597078280024	41.0	40.0	41.0	37.0	41.0
26-27	39.4681119103937	41.0	39.0	41.0	36.5	41.0
28-29	39.38020315203558	41.0	39.0	41.0	36.0	41.0
30-31	39.29295347748548	41.0	39.0	41.0	36.0	41.0
32-33	39.339881190322814	41.0	39.0	41.0	36.0	41.0
34-35	39.29444969230025	41.0	39.0	41.0	35.5	41.0
36-37	39.21950655282801	41.0	39.0	41.0	35.0	41.0
38-39	39.13608662078056	41.0	39.0	41.0	35.0	41.0
40-41	39.02871615784694	41.0	39.0	41.0	35.0	41.0
42-43	38.925745952290626	41.0	39.0	41.0	35.0	41.0
44-45	38.799072008543355	41.0	39.0	41.0	35.0	41.0
46-47	38.60634728260692	41.0	39.0	41.0	35.0	41.0
48-49	38.43197978664162	41.0	38.0	41.0	35.0	41.0
50-51	38.3065188917353	41.0	38.0	41.0	34.5	41.0
52-53	38.177580893262245	40.0	38.0	41.0	34.0	41.0
54-55	38.042806620373185	40.0	38.0	41.0	34.0	41.0
56-57	37.88649100070528	40.0	37.0	41.0	34.0	41.0
58-59	37.69644825846561	40.0	37.0	41.0	34.0	41.0
60-61	37.50252994339227	40.0	36.5	41.0	33.0	41.0
62-63	37.2674148892561	39.5	36.0	41.0	33.0	41.0
64-65	37.013838759438414	39.0	35.5	41.0	33.0	41.0
66-67	36.72943172935662	39.0	35.0	41.0	33.0	41.0
68-69	36.40502736907347	38.0	35.0	40.5	32.0	41.0
70-71	36.06666039833833	37.0	35.0	40.0	32.0	41.0
72-73	35.70794894355631	37.0	35.0	39.5	32.0	41.0
74-75	35.37080938910715	36.0	35.0	39.0	31.0	41.0
76-77	35.03114698405504	36.0	35.0	39.0	31.0	40.5
78-79	34.691006270389494	35.0	35.0	37.5	31.0	39.5
80-81	34.35221391416124	35.0	35.0	37.0	31.0	39.0
82-83	34.03221908644146	35.0	34.5	36.5	31.0	39.0
84-85	33.7663242364976	35.0	34.0	36.0	31.0	37.5
86-87	33.539738170591406	35.0	34.0	36.0	30.5	37.0
88-89	33.32481374153366	35.0	34.0	35.5	30.0	37.0
90-91	33.135703428290626	35.0	34.0	35.0	30.0	36.0
92-93	32.979497292191276	35.0	34.0	35.0	30.0	36.0
94-95	32.834299528856924	35.0	34.0	35.0	30.0	36.0
96-97	32.71433718247492	35.0	34.0	35.0	30.0	36.0
98-99	32.611287319438496	35.0	34.0	35.0	30.0	35.5
100	32.52096974070581	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1846572768438577
1101	2	0.15580464223879886
1101	3	0.17106169137426264
1101	4	0.07744149979423298
1101	5	0.09029004989710643
1101	6	0.11065526474129683
1101	7	0.1529118026865106
1101	8	0.1375193076491712
1101	9	0.22043690419300077
1101	10-11	0.20312577835851187
1101	12-13	0.21236191968849027
1101	14-15	0.23746497260683697
1101	16-17	0.23825011450727374
1101	18-19	0.259408995812322
1101	20-21	0.24663731350138818
1101	22-23	0.29215549360050375
1101	24-25	0.24622371628116468
1101	26-27	0.21476827770037943
1101	28-29	0.24291539848688615
1101	30-31	0.28256892480256823
1101	32-33	0.25209418414849694
1101	34-35	0.3335733389539399
1101	36-37	0.32899518422702556
1101	38-39	0.35678192177039847
1101	40-41	0.40218721638601096
1101	42-43	0.4213916250484573
1101	44-45	0.35365965436601243
1101	46-47	0.4343090286615805
1101	48-49	0.3585978096031255
1101	50-51	0.337166469825938
1101	52-53	0.506949673619367
1101	54-55	0.39480516123345666
1101	56-57	0.3677862660796407
1101	58-59	0.35683518684992066
1101	60-61	0.39381438854861983
1101	62-63	0.33096263728225495
1101	64-65	0.3990621999826942
1101	66-67	0.4088411421032774
1101	68-69	0.39604907857356153
1101	70-71	0.3317118351596733
1101	72-73	0.36105467793272794
1101	74-75	0.33591369685360206
1101	76-77	0.2801166962031658
1101	78-79	0.31407234702279396
1101	80-81	0.37376024653481466
1101	82-83	0.3387266193470069
1101	84-85	0.307325292248521
1101	86-87	0.26721957464710755
1101	88-89	0.3038075562992262
1101	90-91	0.3337229266751365
1101	92-93	0.26776158428502583
1101	94-95	0.29297420629738014
1101	96-97	0.39575492191882233
1101	98-99	0.3631100220025374
1101	100	0.40607868616601195
1102	1	0.1521691841927435
1102	2	0.1514556544970418
1102	3	0.174983902632178
1102	4	0.059879683108626125
1102	5	0.11258737559738563
1102	6	0.11445571289223011
1102	7	0.1171730126940318
1102	8	0.1627473552001959
1102	9	0.21716070001001953
1102	10-11	0.14306261686386534
1102	12-13	0.22958384702248935
1102	14-15	0.25305200401659533
1102	16-17	0.18708670328782517
1102	18-19	0.2527994820453898
1102	20-21	0.2648113300095858
1102	22-23	0.28965414879146323
1102	24-25	0.2374007358993424
1102	26-27	0.27876415955667966
1102	28-29	0.2634176267731192
1102	30-31	0.3020762437698963
1102	32-33	0.2952765188794828
1102	34-35	0.2612669291131411
1102	36-37	0.2557752660911561
1102	38-39	0.26992293256805766
1102	40-41	0.2965332829531633
1102	42-43	0.25186560343889397
1102	44-45	0.2839235219299354
1102	46-47	0.3472610180883535
1102	48-49	0.2457110478676654
1102	50-51	0.2415208368038364
1102	52-53	0.1832979560043313
1102	54-55	0.22037544932539532
1102	56-57	0.23822875278295186
1102	58-59	0.2593281243759691
1102	60-61	0.17014286394485367
1102	62-63	0.15352489926887358
1102	64-65	0.10839407035378912
1102	66-67	0.12912111175155871
1102	68-69	0.11737105257510905
1102	70-71	0.1527227753147784
1102	72-73	0.22607884887131746
1102	74-75	0.17707126572386045
1102	76-77	0.11758542716654574
1102	78-79	0.16262679308615446
1102	80-81	0.20208979777827096
1102	82-83	0.20607348033115613
1102	84-85	0.2112984459414875
1102	86-87	0.2043051686252113
1102	88-89	0.20957890670124613
1102	90-91	0.18658546403589327
1102	92-93	0.19654313386127598
1102	94-95	0.20326806933704233
1102	96-97	0.18446048533147064
1102	98-99	0.2693570607508491
1102	100	0.18016031518396147
1103	1	0.1536606618299885
1103	2	0.18850000363077868
1103	3	0.19182504264254874
1103	4	0.13817531320574972
1103	5	0.07006941644792875
1103	6	0.11713640357741895
1103	7	0.1468123241986632
1103	8	0.13195879499448893
1103	9	0.19640189066014102
1103	10-11	0.17122649805889978
1103	12-13	0.173621101458167
1103	14-15	0.2633442261950947
1103	16-17	0.2272406412986001
1103	18-19	0.2418493194336122
1103	20-21	0.1904156453378718
1103	22-23	0.2094681503414506
1103	24-25	0.2190222003120894
1103	26-27	0.2675196020472015
1103	28-29	0.2751006287525257
1103	30-31	0.2935363966129998
1103	32-33	0.29702584867259674
1103	34-35	0.3110061857010109
1103	36-37	0.30347254300679083
1103	38-39	0.3643479403949854
1103	40-41	0.32580244522449675
1103	42-43	0.27063502318599575
1103	44-45	0.30909822052930025
1103	46-47	0.38290223542699664
1103	48-49	0.32978676114399974
1103	50-51	0.3121127573678564
1103	52-53	0.34408482251554773
1103	54-55	0.3236678203437222
1103	56-57	0.37873053116216937
1103	58-59	0.43038415144770426
1103	60-61	0.44599238859836987
1103	62-63	0.37183062310086257
1103	64-65	0.36923335585422024
1103	66-67	0.3943800502670243
1103	68-69	0.40763336992554855
1103	70-71	0.40777855603095503
1103	72-73	0.42202462327463763
1103	74-75	0.369916347423235
1103	76-77	0.3377812114069769
1103	78-79	0.2971373068230392
1103	80-81	0.2794169767846739
1103	82-83	0.24644058433597138
1103	84-85	0.2916341396120359
1103	86-87	0.2971506951332401
1103	88-89	0.26935609178098474
1103	90-91	0.25601092522548186
1103	92-93	0.3364731620381889
1103	94-95	0.352041304173774
1103	96-97	0.35373584070792674
1103	98-99	0.3939970651304989
1103	100	0.34420246251485764
1104	1	0.042403261927802305
1104	2	0.10819265383262433
1104	3	0.06667570000349343
1104	4	0.03697360228295565
1104	5	-0.022791516608961615
1104	6	-0.030455273624617973
1104	7	0.0021999973258601813
1104	8	0.05274277588902265
1104	9	0.04853960553799652
1104	10-11	0.06189151215084365
1104	12-13	0.10190798898446474
1104	14-15	0.1448174700156244
1104	16-17	0.15548585936240045
1104	18-19	0.09562386795975897
1104	20-21	0.08921542840982966
1104	22-23	0.09231947441379162
1104	24-25	0.0908524874442378
1104	26-27	0.02301602500153166
1104	28-29	0.14446131007129281
1104	30-31	0.12451446886219486
1104	32-33	0.05503543063532135
1104	34-35	0.06637341058691248
1104	36-37	0.10138623778768618
1104	38-39	0.13336920559158472
1104	40-41	0.14900999091133826
1104	42-43	0.12438414132311237
1104	44-45	0.09296654580730035
1104	46-47	0.05541981860856282
1104	48-49	-0.03011628379269382
1104	50-51	0.017476822130056746
1104	52-53	0.01738301795824526
1104	54-55	-0.042268495076768886
1104	56-57	0.0509022545572293
1104	58-59	0.09155062228197863
1104	60-61	0.039250464435752974
1104	62-63	-0.001878005180060427
1104	64-65	0.03754709761150821
1104	66-67	0.08904720999526461
1104	68-69	0.061302033197122796
1104	70-71	0.09326586496770517
1104	72-73	0.125309924243453
1104	74-75	0.017816144805067324
1104	76-77	-0.05908319889023517
1104	78-79	-0.04359434268292972
1104	80-81	0.017574064097686914
1104	82-83	0.0473371370859752
1104	84-85	-0.034686663803789486
1104	86-87	-0.03495647989350914
1104	88-89	-0.00893459893630677
1104	90-91	-0.03450171439238403
1104	92-93	0.019030682297895396
1104	94-95	-0.025227968309891935
1104	96-97	0.018101603882342943
1104	98-99	-0.01490176524698228
1104	100	0.016677174542493844
1105	1	0.08078655282861291
1105	2	0.10166137382346818
1105	3	0.04898163807383327
1105	4	0.0583563068101256
1105	5	0.09164488484652367
1105	6	0.08013264467899006
1105	7	-0.010413784404178728
1105	8	0.017725697069508328
1105	9	0.06162003670507943
1105	10-11	-0.014807677923698748
1105	12-13	0.08439685638354177
1105	14-15	0.07602292024110113
1105	16-17	0.06821151073453535
1105	18-19	0.04769254091074515
1105	20-21	0.09038280184085323
1105	22-23	0.17705474521293496
1105	24-25	0.03509197258518526
1105	26-27	0.0615378485485536
1105	28-29	0.05620517301882444
1105	30-31	0.038635069622728224
1105	32-33	0.06108050292224476
1105	34-35	0.11421078913183891
1105	36-37	0.038837060885306585
1105	38-39	-0.037085842595530494
1105	40-41	0.11121532316224148
1105	42-43	0.08300752673356016
1105	44-45	0.1304999573571024
1105	46-47	0.12690560092247694
1105	48-49	0.051799813288383234
1105	50-51	0.054868795409809934
1105	52-53	0.14042390889625267
1105	54-55	0.1171597106448985
1105	56-57	0.05770438244866938
1105	58-59	0.06220697708751288
1105	60-61	0.08329303576163483
1105	62-63	0.07286123226427321
1105	64-65	0.0420952737295579
1105	66-67	0.01869259016584124
1105	68-69	0.04047889558328421
1105	70-71	0.005521117710735268
1105	72-73	-0.03580682384733791
1105	74-75	-0.06424820157828748
1105	76-77	-0.07650888115570353
1105	78-79	-0.03498607276510057
1105	80-81	0.018167404982733615
1105	82-83	0.03306596252409122
1105	84-85	0.06441254281718756
1105	86-87	0.050203355661935234
1105	88-89	0.07301152292443192
1105	90-91	0.11462592985027698
1105	92-93	0.13554613571768925
1105	94-95	0.11586125196313901
1105	96-97	0.10305817074218027
1105	98-99	0.052381266509677005
1105	100	0.04920847654690874
1106	1	0.07274055473952501
1106	2	0.0692355407052645
1106	3	0.04131822706885657
1106	4	0.03446908390891679
1106	5	-0.019345974025164026
1106	6	0.00878021303026344
1106	7	-0.009596931806299835
1106	8	0.032317351741383504
1106	9	0.07742268130704133
1106	10-11	0.043828347323952244
1106	12-13	0.035098432742351804
1106	14-15	0.07568450987912456
1106	16-17	0.043919731462871425
1106	18-19	0.06843934661223017
1106	20-21	0.06488247367092015
1106	22-23	0.16912228487196046
1106	24-25	0.15363770585209835
1106	26-27	0.10160958868749503
1106	28-29	0.04422524377778814
1106	30-31	0.12471579418765089
1106	32-33	0.10022703246563225
1106	34-35	0.11731709142350155
1106	36-37	0.1443422068185214
1106	38-39	0.1210029390414391
1106	40-41	0.1020557382662517
1106	42-43	0.13876444426049517
1106	44-45	0.1694164345362026
1106	46-47	0.23010444808863895
1106	48-49	0.22379181908780055
1106	50-51	0.11974957541593056
1106	52-53	0.15937375795713393
1106	54-55	0.19624806980474574
1106	56-57	0.20771513489612659
1106	58-59	0.13589285654007455
1106	60-61	0.1759466254834834
1106	62-63	0.14502116914514573
1106	64-65	0.14746241205914146
1106	66-67	0.15427839255324471
1106	68-69	0.19646201936097896
1106	70-71	0.18893042468632615
1106	72-73	0.21436803738298238
1106	74-75	0.21228134895750372
1106	76-77	0.1822622819947668
1106	78-79	0.1796315878262149
1106	80-81	0.17695920378264418
1106	82-83	0.12690946595011354
1106	84-85	0.17579420492884879
1106	86-87	0.1685096891987996
1106	88-89	0.029920345504045542
1106	90-91	0.08347104078505652
1106	92-93	0.12166738141075228
1106	94-95	0.0577980356691441
1106	96-97	0.08260399117178707
1106	98-99	0.08619592765635531
1106	100	0.1114608606610119
1107	1	-0.008864974957589311
1107	2	-0.005964399805399978
1107	3	0.002547797304870869
1107	4	-0.005332944010945084
1107	5	-0.09893313979013385
1107	6	-0.12044693909115978
1107	7	-0.09837666244701637
1107	8	-0.03942148372083665
1107	9	-0.06315414761883176
1107	10-11	-0.08271778882206604
1107	12-13	-0.02221404010175121
1107	14-15	-0.03976249501751994
1107	16-17	-0.041771864387342816
1107	18-19	-0.0011321181846000172
1107	20-21	-0.0747161478722731
1107	22-23	-0.05321313296865071
1107	24-25	-0.00868784319718685
1107	26-27	0.005051241810932083
1107	28-29	-0.013981496178203656
1107	30-31	-0.02779251227175905
1107	32-33	-0.05934656796213034
1107	34-35	-0.031828106427852276
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2216	18-19	-0.2800667841017912
2216	20-21	-0.25741289069922146
2216	22-23	-0.2885796286043245
2216	24-25	-0.3847186116959733
2216	26-27	-0.38852883343945166
2216	28-29	-0.43819056044459614
2216	30-31	-0.2897998323534665
2216	32-33	-0.29900330023217947
2216	34-35	-0.3336284965977043
2216	36-37	-0.34197737565830266
2216	38-39	-0.38693053251360965
2216	40-41	-0.44116431819887225
2216	42-43	-0.4124666095403313
2216	44-45	-0.33837907613425244
2216	46-47	-0.43446991478431585
2216	48-49	-0.36370137810216363
2216	50-51	-0.25664560550740845
2216	52-53	-0.25671704213157653
2216	54-55	-0.24266362266849484
2216	56-57	-0.1977299753068209
2216	58-59	-0.18146031652938177
2216	60-61	-0.21021973804673877
2216	62-63	-0.16097602139468847
2216	64-65	-0.10988791736880188
2216	66-67	-0.08594265167063497
2216	68-69	-0.1154065908107782
2216	70-71	-0.21497482583725258
2216	72-73	-0.13207552662849764
2216	74-75	-0.08206422123173951
2216	76-77	-0.10440627974654149
2216	78-79	-0.10447054111614307
2216	80-81	-0.05311594989445467
2216	82-83	-0.16374287076752125
2216	84-85	-0.09978822165485468
2216	86-87	-0.11142004958770713
2216	88-89	-0.07069771925890933
2216	90-91	-0.0751613776754283
2216	92-93	-0.12751911267164928
2216	94-95	-0.21028714344011945
2216	96-97	-0.19195217115998986
2216	98-99	-0.2067081057227682
2216	100	-0.19064238391131028
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13.0
3	7.0
4	11.0
5	19.0
6	63.0
7	105.0
8	260.0
9	474.0
10	919.0
11	1483.0
12	2253.0
13	3424.0
14	4352.0
15	5767.0
16	7367.0
17	10433.0
18	12420.0
19	6753.0
20	4821.0
21	4569.0
22	4622.0
23	5263.0
24	5865.0
25	7177.0
26	9202.0
27	11829.0
28	15384.0
29	20116.0
30	25899.0
31	32887.0
32	43001.0
33	55781.0
34	78391.0
35	121433.0
36	229185.0
37	535548.0
38	989837.0
39	490306.0
40	2032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.024173612542114	19.72771828446646	13.592641971461173	23.655466131530257
2	15.627602993993245	20.018965255099776	40.45910175837814	23.89432999252884
3	18.486064123907756	25.136518007864627	29.971872543667033	26.40554532456058
4	12.307812060109221	16.510394425849555	38.65244563813853	32.5293478759027
5	12.580233400964694	38.014039571622774	35.79068288266784	13.615044144744695
6	31.194082782125403	37.984261248006284	17.441134266794943	13.380521703073367
7	27.65901027294863	32.50263171104504	22.102626182087487	17.735731833918845
8	25.38882266907362	36.3813402947192	19.91920737972859	18.310629656478593
9	25.33161554275538	16.49617279945062	21.031634602374158	37.14057705541984
10-11	24.230105764920165	26.579867473308706	28.62958453327853	20.5604422284926
12-13	25.00375554101433	23.851104529164278	29.01623739529497	22.128902534526425
14-15	21.986153410233776	25.822118534053963	26.425660784739506	25.76606727097276
16-17	21.279422799716723	29.4010848694072	26.9901002847664	22.329392046109678
18-19	21.129587948501204	27.92315991020155	29.650165062118354	21.297087079178894
20-21	22.189227617066486	27.1957729885486	29.347343350291766	21.267656044093144
22-23	21.818920858010358	26.97094771607346	29.473487028817676	21.7366443970985
24-25	21.670538301174187	27.18711279487151	29.312713795428202	21.8296351085261
26-27	21.424140318536406	27.148633044773803	29.434454477352844	21.992772159336948
28-29	21.473763778885964	27.212615444720218	29.472292721503447	21.841328054890376
30-31	21.714841071493222	27.538123174891492	29.15279670617661	21.594239047438677
32-33	21.43715901568398	27.31372712741196	29.536666086364594	21.712447770539463
34-35	21.576749898262523	27.115070772355278	29.544399836201297	21.763779493180905
36-37	21.880298221180226	27.39428661657505	29.027266601760047	21.698148560484675
38-39	21.701454200153535	27.61630508733569	29.033631789390196	21.648608923120584
40-41	21.556661276323403	27.372992651996125	29.24152976595577	21.828816305724697
42-43	21.58776911857425	27.286805263240232	29.111865501924765	22.013560116260752
44-45	21.66957837155052	27.502986892598475	28.962562339104352	21.86487239674665
46-47	21.918539285478474	27.640503025541808	28.665660305149814	21.77529738382991
48-49	21.941274388975142	27.554012603910966	28.439047387620462	22.065665619493426
50-51	21.85099404959808	27.674934468461558	28.350238518066917	22.123832963873443
52-53	21.8998664845235	27.65703709860557	28.36309953059516	22.07999688627577
54-55	21.962362623722083	27.967279406348606	28.198423115753187	21.87193485417613
56-57	21.791224583997618	27.73247373875265	28.22076070773966	22.255540969510072
58-59	21.732217848687917	27.344626521125758	28.6226895192978	22.300466110888525
60-61	21.974762949115423	27.646343013725737	28.30515869501208	22.073735342146765
62-63	21.73870897282311	27.674081047071553	28.384025415120856	22.20318456498448
64-65	21.779582673366097	27.5062159229111	28.42814218517858	22.28605921854423
66-67	21.695364435935474	27.568518095171584	28.30779096377183	22.428326505121113
68-69	22.086079472552125	27.644909672260155	28.12513355971117	22.143877295476546
70-71	22.06175266199098	27.484433981823457	28.179294195015526	22.27451916117003
72-73	21.870967084865264	27.58459391863678	28.23917196386508	22.305267032632877
74-75	21.882879415928404	27.653358077033317	28.18171997328728	22.282042533751
76-77	21.939042836459357	27.687974775744078	27.987310032868862	22.385672354927703
78-79	21.967580842838434	27.784253235268498	28.10715719211958	22.14100872977349
80-81	21.899448757749855	27.636114642383635	28.16340486206213	22.30103173780438
82-83	21.787144285063718	27.619694375051836	28.304462450150076	22.28869888973437
84-85	21.811905749183417	27.567636382153744	28.113874278896866	22.506583589765974
86-87	21.91178369491482	27.63140330233717	28.073230481601446	22.38358252114656
88-89	21.65835961605822	27.594478681803285	28.14271492333786	22.604446778800636
90-91	21.66897030848189	27.48771492068804	28.190828838200687	22.65248593262939
92-93	21.708576875211076	27.705840512965167	28.07211949497149	22.513463116852268
94-95	21.541055792608297	27.454332439399394	28.209478076188198	22.795133691804118
96-97	21.552404255528103	27.39868132315803	28.177324097915413	22.871590323398458
98-99	21.491715440202146	27.558814682146647	28.071405110663882	22.878064766987322
100	21.57168209318034	27.498416853049406	27.97316088519466	22.956740168575596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	15.0
2	64.5
3	138.5
4	112.0
5	79.0
6	206.0
7	466.5
8	835.0
9	1073.5
10	955.0
11	769.0
12	799.0
13	931.5
14	1096.5
15	1256.5
16	1402.5
17	1603.5
18	1866.5
19	2222.0
20	2742.0
21	3536.5
22	4667.5
23	6454.5
24	8726.0
25	11262.5
26	14983.0
27	20112.5
28	26419.0
29	33321.0
30	40312.0
31	47833.5
32	56072.0
33	64456.0
34	72031.5
35	78905.0
36	85167.5
37	90918.5
38	95820.0
39	98834.0
40	101009.5
41	102218.0
42	101648.5
43	102063.0
44	101528.0
45	98690.5
46	97359.5
47	94996.0
48	91786.5
49	89930.0
50	87727.0
51	83686.5
52	77915.0
53	73622.5
54	70308.5
55	66562.0
56	61680.5
57	55430.5
58	49690.5
59	45143.0
60	40988.5
61	35765.5
62	30304.0
63	25232.0
64	20350.0
65	16484.0
66	12984.5
67	9675.5
68	7401.0
69	5621.5
70	3892.0
71	2678.5
72	1926.5
73	1382.5
74	1057.5
75	769.5
76	471.5
77	343.0
78	247.0
79	125.5
80	65.0
81	19.5
82	4.0
83	3.5
84	3.0
85	1.5
86	1.0
87	0.5
88	1.0
89	1.0
90	1.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09300647335239051
2	0.0012003181934410975
3	0.0
4	3.2735950730211757E-4
5	0.03779183645410001
6	2.1823967153474506E-4
7	0.003964687366214535
8	3.637327858912417E-4
9	1.0911983576737253E-4
10-11	4.910392609531764E-4
12-13	0.0
14-15	7.274655717824833E-5
16-17	0.0
18-19	1.0911983576737253E-4
20-21	0.0
22-23	1.273064750619346E-4
24-25	5.4559917883686264E-5
26-27	0.0014731177828595289
28-29	0.008293107518320311
30-31	0.03548213326369063
32-33	0.02555222820885973
34-35	0.028207477545865795
36-37	0.02571590796251079
38-39	0.024770202719193557
40-41	0.030462620818391493
42-43	0.022624179282435236
44-45	0.021642100760528884
46-47	0.01544045676108321
48-49	0.01029363784072214
50-51	0.0077475083394834485
52-53	0.0055105517062523125
54-55	0.004201113677043842
56-57	0.0024733829440604436
58-59	0.0011821315541465354
60-61	7.092789324879213E-4
62-63	0.001509491061448653
64-65	0.0029644222050136198
66-67	5.637858181314246E-4
68-69	0.0011821315541465354
70-71	4.7285262165861424E-4
72-73	6.910922931933593E-4
74-75	6.910922931933593E-4
76-77	4.546659823640522E-4
78-79	5.4559917883686264E-5
80-81	9.457052433172285E-4
82-83	6.910922931933593E-4
84-85	4.001060644803659E-4
86-87	2.1823967153474506E-4
88-89	0.0
90-91	3.637327858912417E-5
92-93	1.8186639294562084E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2749271.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.31130973680314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.97050341697647	43.872874395515474
2	15.036579080516136	17.83678398452854
3	5.024008488093921	8.93941570673
4	2.185697611237536	5.185463520444009
5	1.1316747800284808	3.356055669979889
6	0.6593231130077094	2.346319042334011
7	0.4381188811383649	1.8189783262517438
8	0.29981709802564627	1.422603581231086
9	0.21032039295175795	1.1226940173295077
>10	0.9784526738475291	10.014084449654765
>50	0.04707903227379117	1.896418210755154
>100	0.017933542778473536	1.7179053288896895
>500	2.459445621523091E-4	0.07884432462889258
>1k	2.459445619911051E-4	0.39155944172723595
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4233	0.15396808826776262	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3486	0.12679724916168686	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.6373278589124174E-5	0.0
4	0.0	0.0	0.0	7.274655717824835E-5	0.0
5	0.0	0.0	0.0	1.0911983576737251E-4	0.0
6	0.0	0.0	0.0	1.0911983576737251E-4	0.0
7	0.0	0.0	0.0	1.0911983576737251E-4	0.0
8	0.0	0.0	0.0	1.0911983576737251E-4	0.0
9	3.6373278589124174E-5	0.0	0.0	2.546129501238692E-4	0.0
10-11	1.273064750619346E-4	0.0	0.0	3.0917286800755545E-4	0.0
12-13	1.454931143564967E-4	0.0	0.0	3.273595073021175E-4	0.0
14-15	1.454931143564967E-4	3.6373278589124174E-5	0.0	3.4554614659667963E-4	0.0
16-17	1.454931143564967E-4	9.093319647281042E-5	0.0	3.637327858912417E-4	0.0
18-19	2.0005303224018294E-4	1.0911983576737251E-4	0.0	4.18292703774928E-4	0.0
20-21	2.1823967153474503E-4	1.0911983576737251E-4	0.0	4.7285262165861424E-4	0.0
22-23	3.2735950730211757E-4	1.0911983576737251E-4	0.0	5.455991788368625E-4	0.0
24-25	4.001060644803659E-4	1.0911983576737251E-4	0.0	6.183457360151109E-4	0.0
26-27	4.001060644803659E-4	1.0911983576737251E-4	0.0	6.910922931933593E-4	0.0
28-29	4.7285262165861424E-4	1.0911983576737251E-4	0.0	0.0014003712256812806	0.0
30-31	4.7285262165861424E-4	1.0911983576737251E-4	0.0	0.004164740398454717	0.0
32-33	4.7285262165861424E-4	1.0911983576737251E-4	0.0	0.011312089641217618	0.0
34-35	5.455991788368625E-4	1.273064750619346E-4	0.0	0.020878261910157275	0.0
36-37	5.455991788368625E-4	2.727995894184313E-4	0.0	0.03881028825459549	0.0
38-39	6.183457360151109E-4	4.18292703774928E-4	0.0	0.06861819005838275	0.0
40-41	6.183457360151109E-4	4.546659823640521E-4	0.0	0.10615541356235889	0.0
42-43	6.183457360151109E-4	5.092259002477384E-4	0.0	0.14367445042704047	0.0
44-45	7.274655717824834E-4	5.092259002477384E-4	0.0	0.18925016849921306	0.0
46-47	7.638388503716076E-4	5.092259002477384E-4	0.0	0.2431553673682951	0.0
48-49	8.36585407549856E-4	5.092259002477384E-4	0.0	0.302880290811637	0.0
50-51	8.36585407549856E-4	5.274125395423005E-4	0.0	0.3703709092337569	0.0
52-53	8.36585407549856E-4	5.455991788368625E-4	0.0	0.43744323495210186	0.0
54-55	8.36585407549856E-4	5.819724574259868E-4	0.0	0.5095168864764514	0.0
56-57	8.547720468444181E-4	5.819724574259868E-4	0.0	0.596667261975993	0.0
58-59	8.729586861389801E-4	5.819724574259868E-4	0.0	0.6969120177676191	0.0
60-61	9.275186040226663E-4	6.183457360151109E-4	0.0	0.806140973370759	0.0
62-63	9.457052433172285E-4	6.183457360151109E-4	0.0	0.9184616576539745	0.0
64-65	9.457052433172285E-4	6.183457360151109E-4	0.0	1.0336922042243197	0.0
66-67	9.457052433172285E-4	6.910922931933593E-4	0.0	1.164799686898818	0.0
68-69	9.457052433172285E-4	6.910922931933593E-4	0.0	1.3123660781348947	0.0
70-71	9.457052433172285E-4	7.092789324879213E-4	0.0	1.4764459378504338	0.0
72-73	9.457052433172285E-4	7.274655717824834E-4	0.0	1.6495827439346649	0.0
74-75	9.820785219063527E-4	7.274655717824834E-4	0.0	1.8231014694440817	0.0
76-77	0.0010002651612009148	7.638388503716076E-4	0.0	2.006822899597748	0.0
78-79	0.0011457582755574113	7.638388503716076E-4	0.0	2.223189347285153	0.0
80-81	0.0011639449148519736	7.638388503716076E-4	0.0	2.4639986381844494	0.0
82-83	0.0011639449148519736	7.638388503716076E-4	0.0	2.715683539381894	0.0
84-85	0.0012003181934410977	7.638388503716076E-4	0.0	2.9683687057405397	0.0
86-87	0.0012366914720302218	7.638388503716076E-4	0.0	3.2311474569076672	0.0
88	0.0012366914720302218	7.638388503716076E-4	0.0	3.4496417413925364	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4300	0.0	46.95252	1
GTATCAA	7505	0.0	40.19549	1
TCAACGC	8670	0.0	34.435436	4
ATCAACG	8705	0.0	34.296986	3
CAACGCA	8900	0.0	33.545532	5
AACGCAG	9230	0.0	32.38239	6
TATCAAC	9405	0.0	32.094833	2
ACGCAGA	10895	0.0	27.304232	7
CGCAGAG	10980	0.0	26.83508	8
GTACATG	8940	0.0	25.689304	1
TACATGG	8850	0.0	24.59782	2
GCAGAGT	12080	0.0	24.19698	9
ACATGGG	9165	0.0	23.341562	3
GAGTACT	7075	0.0	21.454271	12-13
CGCGCGT	155	4.2445834E-5	21.223639	7
TAGCGCG	160	5.4016906E-5	20.569752	4
CAGAGTA	11840	0.0	20.222282	10-11
AGAGTAC	10805	0.0	19.636799	10-11
GTACTTT	7895	0.0	19.077154	14-15
GAGTACA	6680	0.0	18.881145	1
>>END_MODULE