FastQCFastQC Report
Wed 25 May 2016
SRR1033137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3898019
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48500.030.497771
TCAACGC45700.030.3243484
GTACATG109150.029.3039531
ATCAACG47950.029.1044523
GGTATCA33700.028.934811
TACATGG108350.028.5044142
CAACGCA49050.028.0613615
ACATGGG109500.027.2061273
AACGCAG52450.026.4119576
TATCAAC61900.023.238412
ACGCAGA59400.022.9263867
CGCAGAG61100.022.135078
CATGGGG66350.021.5945724
TACACCG4550.020.6489855
GAGTACA75950.019.785321
AGTACAT77600.017.8704682
TATACCG2403.4390105E-617.6161675
CATGGGC37250.017.529694
GTCTTAC15200.016.735181
GTATAGA18000.016.2255481
Produced by FastQC (version 0.11.5)