##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033137_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3898019
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.677681663429553	33.0	31.0	34.0	30.0	34.0
2	31.86168230580713	34.0	31.0	34.0	30.0	34.0
3	31.949575669077035	34.0	31.0	34.0	30.0	34.0
4	35.35548261822223	37.0	35.0	37.0	33.0	37.0
5	35.44800961719273	37.0	35.0	37.0	35.0	37.0
6	35.53252921548099	37.0	37.0	37.0	35.0	37.0
7	35.4555103502574	37.0	37.0	37.0	35.0	37.0
8	35.45034541904491	37.0	37.0	37.0	35.0	37.0
9	37.14032307179621	39.0	38.0	39.0	35.0	39.0
10-11	37.12737393019377	39.0	38.0	39.0	35.0	39.0
12-13	37.08279333682057	39.0	38.0	39.0	35.0	39.0
14-15	38.554962918343904	41.0	39.0	41.0	35.0	41.0
16-17	38.57083546796463	41.0	39.0	41.0	35.0	41.0
18-19	38.56538564845374	41.0	39.0	41.0	35.0	41.0
20-21	38.58933858454769	41.0	39.0	41.0	35.0	41.0
22-23	38.55426294740995	41.0	39.0	41.0	34.0	41.0
24-25	38.55309761188953	41.0	39.0	41.0	34.0	41.0
26-27	38.5107130057601	41.0	39.0	41.0	34.0	41.0
28-29	38.48339323127979	40.5	39.0	41.0	34.0	41.0
30-31	38.4823899011267	41.0	39.0	41.0	34.0	41.0
32-33	38.5528844266793	41.0	39.0	41.0	34.0	41.0
34-35	38.52676962323683	41.0	39.0	41.0	34.0	41.0
36-37	38.45171188236897	41.0	39.0	41.0	34.0	41.0
38-39	38.38137474445353	41.0	39.0	41.0	34.0	41.0
40-41	38.32892630846592	41.0	39.0	41.0	34.0	41.0
42-43	38.226101899452004	40.5	38.5	41.0	33.5	41.0
44-45	38.11436655388289	40.0	38.0	41.0	33.0	41.0
46-47	38.002739724973125	40.0	38.0	41.0	33.0	41.0
48-49	37.8999898409936	40.0	38.0	41.0	33.0	41.0
50-51	37.796003046675764	40.0	38.0	41.0	33.0	41.0
52-53	37.66432641811136	40.0	38.0	41.0	32.5	41.0
54-55	37.528945856857035	40.0	37.0	41.0	32.0	41.0
56-57	37.39092985436962	40.0	37.0	41.0	32.0	41.0
58-59	37.21130104804517	40.0	37.0	41.0	31.5	41.0
60-61	37.0087013429129	40.0	36.0	41.0	31.0	41.0
62-63	36.76461979790247	39.0	36.0	41.0	31.0	41.0
64-65	36.508808063788294	39.0	35.0	41.0	31.0	41.0
66-67	36.22886561096803	39.0	35.0	41.0	31.0	41.0
68-69	35.926771393366735	38.0	35.0	40.0	30.0	41.0
70-71	35.58240352343075	37.0	35.0	40.0	30.0	41.0
72-73	35.2182181513225	37.0	35.0	39.5	30.0	41.0
74-75	34.84400512157586	36.5	34.5	39.0	29.0	41.0
76-77	34.50287941131123	36.0	34.0	39.0	29.0	40.5
78-79	34.116244174284425	35.5	34.0	37.5	29.0	39.5
80-81	33.739933797141575	35.0	34.0	37.0	29.0	39.0
82-83	33.494072630225766	35.0	34.0	37.0	29.0	39.0
84-85	33.20621538786753	35.0	34.0	36.0	29.0	38.0
86-87	32.92506270492781	35.0	34.0	36.0	29.0	37.0
88-89	32.543929621687326	35.0	34.0	36.0	27.5	37.0
90-91	32.33686739341188	35.0	34.0	35.0	27.0	36.5
92-93	32.14569413335337	35.0	34.0	35.0	27.0	36.0
94-95	31.962098953340146	35.0	34.0	35.0	27.0	36.0
96-97	31.780421285786446	35.0	34.0	35.0	26.0	36.0
98-99	31.588137846429174	35.0	33.0	35.0	25.5	36.0
100	31.42547406772517	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20160547403073892
1101	2	0.1838922313747986
1101	3	0.19201392603551426
1101	4	0.17279495564426384
1101	5	0.16352648112424362
1101	6	0.17604827219273034
1101	7	0.2461237101790772
1101	8	0.2175431811511075
1101	9	0.3071576742627826
1101	10-11	0.2956474478764619
1101	12-13	0.2630150192834577
1101	14-15	0.3136285136279966
1101	16-17	0.29004756037427626
1101	18-19	0.2636316049299907
1101	20-21	0.23719953538598304
1101	22-23	0.29852841003480535
1101	24-25	0.2624802278637759
1101	26-27	0.2703566793642267
1101	28-29	0.31678741719898795
1101	30-31	0.3204555006875509
1101	32-33	0.29534715730508765
1101	34-35	0.321228485663525
1101	36-37	0.29342054225156033
1101	38-39	0.325152612660915
1101	40-41	0.3181187956180196
1101	42-43	0.30949716664319027
1101	44-45	0.278665656643895
1101	46-47	0.3163786234923194
1101	48-49	0.24423081730656548
1101	50-51	0.20926690365585188
1101	52-53	0.2232573122711088
1101	54-55	0.2109076950874993
1101	56-57	0.23137348562519122
1101	58-59	0.24399979943807892
1101	60-61	0.2862085421401801
1101	62-63	0.2891201539165067
1101	64-65	0.19685449828578072
1101	66-67	0.1623913880704535
1101	68-69	0.220879638334317
1101	70-71	0.23134094412875328
1101	72-73	0.22688916534662695
1101	74-75	0.19513951222923964
1101	76-77	0.12531525701803758
1101	78-79	0.1682458799195743
1101	80-81	0.10100002010278786
1101	82-83	0.15860690774741215
1101	84-85	0.13499884289366548
1101	86-87	0.1985510427203181
1101	88-89	0.3943484100096981
1101	90-91	0.4029639785673709
1101	92-93	0.38067549713518645
1101	94-95	0.4038875551949026
1101	96-97	0.353143462811385
1101	98-99	0.4524132762630799
1101	100	0.5396163205717919
1102	1	0.21197006395330575
1102	2	0.2387716765486303
1102	3	0.2408745668180714
1102	4	0.19864516369290897
1102	5	0.101840944994386
1102	6	0.14878697376475714
1102	7	0.16348141699959484
1102	8	0.12647875100387296
1102	9	0.1542996271412136
1102	10-11	0.13385689947649126
1102	12-13	0.1559432610036069
1102	14-15	0.2009560485776234
1102	16-17	0.21113075734979958
1102	18-19	0.23573583773333695
1102	20-21	0.21912505637335045
1102	22-23	0.23491232520334648
1102	24-25	0.23617619197982265
1102	26-27	0.27429237908156523
1102	28-29	0.24341932274805345
1102	30-31	0.3386191394148952
1102	32-33	0.3258418743301661
1102	34-35	0.30736572096669335
1102	36-37	0.27997851086789893
1102	38-39	0.20627756040089906
1102	40-41	0.2863302677992081
1102	42-43	0.2955474240962275
1102	44-45	0.22491777881267438
1102	46-47	0.269199537024015
1102	48-49	0.2627783420178318
1102	50-51	0.2752437136120278
1102	52-53	0.18181747224751632
1102	54-55	0.24221108095035504
1102	56-57	0.1943688177615357
1102	58-59	0.22842840785112628
1102	60-61	0.14037770485994372
1102	62-63	0.21867401252833218
1102	64-65	0.30168796022693556
1102	66-67	0.1997706384474185
1102	68-69	0.15352388995545851
1102	70-71	0.2037830517554866
1102	72-73	0.26776032418273843
1102	74-75	0.22002140877156506
1102	76-77	0.22339775063023382
1102	78-79	0.2894904196725179
1102	80-81	0.2566937406336507
1102	82-83	0.2099512959078922
1102	84-85	0.19919694446899427
1102	86-87	0.17514687942943397
1102	88-89	0.27761921284929514
1102	90-91	0.2903124943269262
1102	92-93	0.3843094187969598
1102	94-95	0.342207307904804
1102	96-97	0.28850293146137673
1102	98-99	0.249332825574335
1102	100	0.2765028605408979
1103	1	0.12208979746977988
1103	2	0.09697810309188881
1103	3	0.07409334715912053
1103	4	0.09873855626382522
1103	5	0.06859244062660963
1103	6	0.09688289702486941
1103	7	0.06243561149853605
1103	8	0.08804543061069836
1103	9	0.15138170291099584
1103	10-11	0.12451237513181468
1103	12-13	0.10492222663958017
1103	14-15	0.1137541550153216
1103	16-17	0.07639174085925049
1103	18-19	0.1265011264108935
1103	20-21	0.1309241405469237
1103	22-23	0.08061616661242255
1103	24-25	0.12534266044171716
1103	26-27	0.17428577745816654
1103	28-29	0.16766617918474935
1103	30-31	0.21598513916821815
1103	32-33	0.1571981541127201
1103	34-35	0.1858980576845397
1103	36-37	0.19019609823473616
1103	38-39	0.1885105150360431
1103	40-41	0.17997956963238693
1103	42-43	0.13601493073196025
1103	44-45	0.09163695525112558
1103	46-47	0.05582715958077955
1103	48-49	0.10368610737300088
1103	50-51	0.054803920417406005
1103	52-53	0.02331542072782611
1103	54-55	0.03365260461306718
1103	56-57	-0.022411003985268962
1103	58-59	-0.013948222054239068
1103	60-61	-0.014158841111836296
1103	62-63	0.04313321784002966
1103	64-65	0.10735228687338605
1103	66-67	0.15457082128629906
1103	68-69	0.04302998752785214
1103	70-71	0.12056373632844952
1103	72-73	0.12264381656591894
1103	74-75	0.09774317382226627
1103	76-77	0.036399678009971126
1103	78-79	0.03304373711200981
1103	80-81	0.02896871824699332
1103	82-83	0.019388062388607352
1103	84-85	0.012797833728399155
1103	86-87	0.03283144663984672
1103	88-89	0.06427634874268051
1103	90-91	0.12998986289174752
1103	92-93	0.11610096093350819
1103	94-95	0.07744396774288376
1103	96-97	0.08551948260744169
1103	98-99	0.17384266005350923
1103	100	0.17545026613634462
1104	1	0.011985427521931769
1104	2	-0.010383327176906931
1104	3	-0.03305786179507919
1104	4	-0.010226812161640453
1104	5	-0.0014150828467123233
1104	6	-0.04145189040084318
1104	7	-0.08836791358402962
1104	8	-0.09011184502394087
1104	9	-0.02566039349735405
1104	10-11	-0.0050478183450906045
1104	12-13	-0.005682234707435896
1104	14-15	-0.09594504622084088
1104	16-17	-0.055878921960612615
1104	18-19	-0.02329520742453184
1104	20-21	-0.009521057980890646
1104	22-23	0.007042483993430437
1104	24-25	0.028873082928370764
1104	26-27	0.05955406312793343
1104	28-29	0.03893242005921849
1104	30-31	0.002093310267220261
1104	32-33	-0.03650894043710906
1104	34-35	-0.012784569341832253
1104	36-37	-0.008985832544041727
1104	38-39	-0.07046065304154325
1104	40-41	-0.006339848329439235
1104	42-43	0.00890881953822742
1104	44-45	-0.08845995680705698
1104	46-47	-0.11177701570579757
1104	48-49	-0.1752178606113759
1104	50-51	-0.07926882998143725
1104	52-53	-0.1224281490043353
1104	54-55	-0.09178978764184365
1104	56-57	-0.09601934306198956
1104	58-59	-0.07367139347118723
1104	60-61	-9.433339480153791E-4
1104	62-63	-0.07779308111969385
1104	64-65	-0.14878052374540118
1104	66-67	-0.11326502181112375
1104	68-69	-0.12600690115296942
1104	70-71	-0.10068630474170703
1104	72-73	-0.05038467303555905
1104	74-75	-0.09606864559868455
1104	76-77	-0.14202099313323657
1104	78-79	-0.10846854596604771
1104	80-81	0.013503032866033493
1104	82-83	0.013916650011935872
1104	84-85	0.0019804377040344434
1104	86-87	0.01640028314154307
1104	88-89	0.1626095306549331
1104	90-91	0.09126455055444183
1104	92-93	0.0872874276574862
1104	94-95	0.1750878944365688
1104	96-97	0.19940711286894341
1104	98-99	0.2472201382554644
1104	100	0.2980629811213156
1105	1	0.09695529804410441
1105	2	0.15478279440967668
1105	3	0.09576520246656628
1105	4	0.14461558150697584
1105	5	0.11932515888575068
1105	6	0.05975440036436197
1105	7	0.06681983334169672
1105	8	0.07081350901873407
1105	9	0.14507658738889972
1105	10-11	0.08942612193547461
1105	12-13	0.16076460275345994
1105	14-15	0.15409895667539075
1105	16-17	0.21268103647842196
1105	18-19	0.16014156513811173
1105	20-21	0.15557697491364308
1105	22-23	0.18653187253289616
1105	24-25	0.19906637329263788
1105	26-27	0.2217775489280669
1105	28-29	0.2417336166415609
1105	30-31	0.21566011648328498
1105	32-33	0.24271694949599976
1105	34-35	0.19050714373631195
1105	36-37	0.1898375948572948
1105	38-39	0.21596540764132044
1105	40-41	0.21764906701677234
1105	42-43	0.16774463815514196
1105	44-45	0.20844990969834498
1105	46-47	0.20298338991383247
1105	48-49	0.16123393481795034
1105	50-51	0.21318905507817476
1105	52-53	0.1556314325846273
1105	54-55	0.13228324271441494
1105	56-57	0.15168259410298646
1105	58-59	0.1492073519626942
1105	60-61	0.1552745439377432
1105	62-63	0.2058996122873964
1105	64-65	0.2036060636530621
1105	66-67	0.19799942318173436
1105	68-69	0.1411167101156252
1105	70-71	0.16296608015969127
1105	72-73	0.19700942913119235
1105	74-75	0.15196530499801497
1105	76-77	0.1835814482030358
1105	78-79	0.15495900729506218
1105	80-81	0.16779061949151952
1105	82-83	0.17454093160675654
1105	84-85	0.09828341626472081
1105	86-87	0.14954674775432153
1105	88-89	0.1549844308841557
1105	90-91	0.20343441766755177
1105	92-93	0.18021922854133265
1105	94-95	0.22100690835667436
1105	96-97	0.2953161033924836
1105	98-99	0.33242614221443034
1105	100	0.34281068426276917
1106	1	0.032315864306422526
1106	2	0.0996246530983953
1106	3	0.11237574061134836
1106	4	0.062107009506078725
1106	5	0.08440542823635155
1106	6	0.09518462708286535
1106	7	0.03441623742254052
1106	8	0.0666901167202596
1106	9	0.06728305984448468
1106	10-11	-0.007773063638609301
1106	12-13	0.0305735136091414
1106	14-15	0.07240156079728877
1106	16-17	0.07274455724858342
1106	18-19	0.05717624240680408
1106	20-21	0.051055657322336856
1106	22-23	0.022931378800791435
1106	24-25	0.08035576300122216
1106	26-27	0.12160432714617286
1106	28-29	0.14516725321808366
1106	30-31	0.10065143142028887
1106	32-33	0.09544706163701733
1106	34-35	0.10795417562550824
1106	36-37	0.11614212423130255
1106	38-39	0.10787603389166378
1106	40-41	0.12116971996871229
1106	42-43	0.13434852280291665
1106	44-45	0.1612037471205099
1106	46-47	0.18634360953132045
1106	48-49	0.1929191720814245
1106	50-51	0.19681369792626668
1106	52-53	0.08054233847127534
1106	54-55	0.07179836734435696
1106	56-57	0.08235756532838678
1106	58-59	0.03267984638240051
1106	60-61	0.04686569578119304
1106	62-63	0.09380038189099338
1106	64-65	0.11858487901330506
1106	66-67	0.16696831820163283
1106	68-69	0.12743302991252392
1106	70-71	0.16035043894844847
1106	72-73	0.16576504523909819
1106	74-75	0.2306828931740128
1106	76-77	0.1796189178094565
1106	78-79	0.21296687570641382
1106	80-81	0.21780511630127108
1106	82-83	0.12316356301509757
1106	84-85	0.16019018064544355
1106	86-87	0.16174377982109434
1106	88-89	0.2269539879388418
1106	90-91	0.23670216906732833
1106	92-93	0.1918924161086153
1106	94-95	0.2503670621495395
1106	96-97	0.2163771858098933
1106	98-99	0.22584910715002593
1106	100	0.22400030111368707
1107	1	0.06590451173952516
1107	2	0.0788276167051194
1107	3	0.05452997624504974
1107	4	0.06693219661040217
1107	5	0.00411449018449872
1107	6	0.0971360075167027
1107	7	0.09771935348210548
1107	8	0.08190903936559124
1107	9	0.0913390205592961
1107	10-11	0.08600114855552476
1107	12-13	0.020540868568282633
1107	14-15	0.05980441595160357
1107	16-17	0.05716457257129548
1107	18-19	0.09654811688441356
1107	20-21	0.0497076032027195
1107	22-23	0.085291315314457
1107	24-25	0.030933507801449878
1107	26-27	0.08932009210005987
1107	28-29	0.10354149533797141
1107	30-31	0.09500596193886679
1107	32-33	0.09216764021991963
1107	34-35	0.057423312237730784
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2216	20-21	-0.3522740159912985
2216	22-23	-0.31404805586106477
2216	24-25	-0.32665342864942915
2216	26-27	-0.41620590998778084
2216	28-29	-0.36982129409700093
2216	30-31	-0.4503778933148155
2216	32-33	-0.4011184915671606
2216	34-35	-0.3715659858941365
2216	36-37	-0.3222702401653592
2216	38-39	-0.26932087562057916
2216	40-41	-0.3225923661998138
2216	42-43	-0.37838857378375224
2216	44-45	-0.3222470940661495
2216	46-47	-0.26483343746748744
2216	48-49	-0.2218327614629274
2216	50-51	-0.23989192430263273
2216	52-53	-0.23825745101127893
2216	54-55	-0.2550736434427705
2216	56-57	-0.31509912535518225
2216	58-59	-0.2934844387482016
2216	60-61	-0.24843908189347275
2216	62-63	-0.19509425010815562
2216	64-65	-0.14343386511404788
2216	66-67	-0.10920433250341688
2216	68-69	-0.18228043072375755
2216	70-71	-0.28271129724402755
2216	72-73	-0.2297384245333305
2216	74-75	-0.2598262316102691
2216	76-77	-0.28884250262625955
2216	78-79	-0.253878154647424
2216	80-81	-0.2062699927763063
2216	82-83	-0.21298283220841085
2216	84-85	-0.2339981858878275
2216	86-87	-0.1657397816310322
2216	88-89	-0.0853531889670478
2216	90-91	-0.10296288672483911
2216	92-93	-0.13774409191815806
2216	94-95	-0.1507373149868627
2216	96-97	-0.14036310405823116
2216	98-99	-0.19179300134739918
2216	100	-0.16996788680001984
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33812.0
3	6181.0
4	4003.0
5	3220.0
6	2841.0
7	3194.0
8	3774.0
9	4172.0
10	4633.0
11	4975.0
12	5746.0
13	6407.0
14	7334.0
15	7807.0
16	7678.0
17	7948.0
18	7682.0
19	7506.0
20	7615.0
21	7925.0
22	9379.0
23	12358.0
24	19257.0
25	26508.0
26	27080.0
27	22927.0
28	25023.0
29	31472.0
30	38663.0
31	47072.0
32	63160.0
33	86020.0
34	119699.0
35	180779.0
36	332320.0
37	735557.0
38	1329005.0
39	645723.0
40	1564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88247443272199	20.08916758729116	13.134626017105596	23.893731962881244
2	14.968688572465517	20.42474431119868	41.27503944590036	23.33152767043544
3	17.947502169888747	25.999207775607957	30.18813917375052	25.865150880752775
4	11.596336415920018	16.755453683820694	40.228308006127705	31.419901894131584
5	12.174317000899586	38.79434890349273	36.40778854458214	12.623545551025542
6	30.121969085292154	39.2289975413245	18.222703198638555	12.426330174744788
7	26.54935097088188	33.15452909310383	23.29918965966219	16.996930276352096
8	24.295198691426922	37.52176785657013	20.841001956445044	17.342031495557908
9	24.714506160960383	17.04498160512891	21.445304702097513	36.7952075318132
10-11	23.14027860939837	27.549162836222585	29.73759335765265	19.5729651967264
12-13	23.792172050470985	24.906436856873665	29.718209827760685	21.58318126489467
14-15	21.081084687208048	27.021356739113088	26.532655799404246	25.36490277427462
16-17	20.8345435940175	30.36832295331661	27.194711528592602	21.602421924073294
18-19	20.772796995622038	29.443984290445098	29.022827914891696	20.76039079904117
20-21	21.935364177437314	28.730748736961132	28.454963171496793	20.87892391410476
22-23	21.429025947767826	28.559184044206244	28.597620240121024	21.4141697679049
24-25	21.40608996182852	28.707156841273186	28.45401466428382	21.432738532614476
26-27	21.179205105773054	28.642610896878058	28.56505592233264	21.613128075016252
28-29	21.16851729352942	28.672883206003842	28.684134764158614	21.474464736308125
30-31	21.380051282932914	29.15943819195459	28.273880010869334	21.186630514243163
32-33	21.041625903812708	28.787904642827588	28.753955734267034	21.41651371909267
34-35	21.143456299101025	28.61279696350284	28.85286815994979	21.390878577446344
36-37	21.47639762468907	28.900052468252856	28.31809102792876	21.30545887912931
38-39	21.270884191574776	29.036693110707173	28.49007004662377	21.202352651094277
40-41	21.102024788739477	28.7684665743795	28.74382330842452	21.385685328456507
42-43	21.085754016512475	28.64508448658939	28.70236244310689	21.56679905379125
44-45	21.1903186167136	28.784800977923723	28.66188061484812	21.362999790514557
46-47	21.22148501455551	28.936436438683614	28.569306422010815	21.27277212475006
48-49	21.2090141985467	28.770057482005683	28.617613627690393	21.40331469175722
50-51	21.163326280935166	28.718598537584132	28.626634718144174	21.49144046333653
52-53	21.207812144622196	28.69860062362411	28.612421606021293	21.481165625732405
54-55	21.294576102515464	29.098927221927973	28.412975751759838	21.193520923796726
56-57	21.202253013810036	28.72638786625585	28.51478620484416	21.55657291508996
58-59	21.038854221276868	28.45666136593627	28.855415512570197	21.64906890021667
60-61	21.28759907862062	28.79229750030241	28.515337231292676	21.404766189784294
62-63	21.133268956656572	28.805056681919357	28.50971756015816	21.551956801265916
64-65	21.129545724502883	28.604196955248707	28.62836368412803	21.637893636120385
66-67	21.014190847204993	28.65605763051462	28.52999087978635	21.79976064249404
68-69	21.379505499065584	28.765964248088615	28.485940917514462	21.368589335331336
70-71	21.335546647966115	28.568434303948166	28.54098379791605	21.555035250169674
72-73	21.140743055357802	28.697863713922224	28.629224771180006	21.532168459539967
74-75	21.192436271764496	28.79287640405047	28.468369917384123	21.54631740680091
76-77	21.26739593035509	28.74346259619224	28.363689959499556	21.625451513953113
78-79	21.350280531310485	28.81421309040075	28.411929433794832	21.423576944493938
80-81	21.357030414606566	28.59700851543307	28.559487965716606	21.48647310424376
82-83	21.160404390344894	28.638308614575426	28.692722292444913	21.508564702634768
84-85	21.202550399587057	28.609425231387476	28.49278547237884	21.69523889664663
86-87	21.359613476301746	28.693991981372648	28.410417955814427	21.53597658651118
88-89	21.169311908479184	28.550802554484633	28.4778759051951	21.802009631841084
90-91	21.204117293803293	28.546292451864648	28.550680625015247	21.698909629316816
92-93	21.360299128179502	28.695596397906968	28.375826494918165	21.56827797899536
94-95	21.08531495169866	28.509315600000722	28.566067838926873	21.839301609373745
96-97	21.186674698061367	28.479088178623176	28.539697852324537	21.794539270990924
98-99	21.22879689406499	28.53794571426802	28.438007754514693	21.7952496371523
100	21.36448026988483	28.458480070650694	28.294628059289188	21.882411600175285
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	597.0
1	551.5
2	481.5
3	412.5
4	326.0
5	267.0
6	262.5
7	261.0
8	256.5
9	258.5
10	250.5
11	265.0
12	293.5
13	325.5
14	378.5
15	486.0
16	630.5
17	872.5
18	1326.5
19	1896.5
20	2863.5
21	4396.0
22	6753.5
23	10324.0
24	14890.5
25	20709.5
26	28636.5
27	38950.0
28	51377.5
29	64008.0
30	75819.0
31	88003.5
32	101008.5
33	112974.5
34	122901.5
35	131792.5
36	138665.5
37	144490.5
38	148130.5
39	148077.0
40	147853.0
41	147040.5
42	144944.5
43	142656.5
44	139090.5
45	134932.0
46	130619.5
47	125768.0
48	121726.5
49	117577.5
50	112289.0
51	106253.5
52	98271.5
53	92047.5
54	87610.0
55	81736.5
56	74732.5
57	67675.0
58	61119.0
59	54140.0
60	46797.0
61	39314.0
62	32875.5
63	27657.0
64	22954.5
65	18415.0
66	14326.0
67	10765.5
68	8046.0
69	6239.0
70	4776.5
71	3541.0
72	2622.5
73	1988.0
74	1515.0
75	1075.5
76	664.5
77	471.0
78	366.0
79	213.5
80	121.5
81	76.0
82	51.0
83	39.0
84	29.5
85	23.0
86	20.0
87	12.0
88	11.5
89	8.5
90	7.5
91	6.0
92	3.5
93	5.5
94	4.5
95	2.5
96	3.0
97	3.5
98	3.5
99	2.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3328613842056696
2	0.12088191463407438
3	0.06818848240606318
4	0.0414569554432649
5	0.045766836949742935
6	0.010056390181782079
7	0.007029211504612985
8	0.01652121244149913
9	0.018907039704013757
10-11	0.021613542673855617
12-13	0.01670079083760238
14-15	0.019189234326461725
16-17	0.020189742533322695
18-19	0.01983058574111619
20-21	0.024871607860300324
22-23	0.01670079083760238
24-25	0.025641229557885684
26-27	0.02009995333527107
28-29	0.020035818193805625
30-31	0.02093371017432188
32-33	0.02530772682226536
34-35	0.01625184484734425
36-37	0.0108773199925398
38-39	0.013942979754588162
40-41	0.010325757775936957
42-43	0.00920980631443818
44-45	0.009581790134937773
46-47	0.009068709003214197
48-49	0.004887097779667057
50-51	0.0074525034382849345
52-53	0.00817081702269794
54-55	0.003809627403047548
56-57	0.003309373299617062
58-59	0.003296546271323973
60-61	0.004297054478184945
62-63	0.003258065186444704
64-65	0.0025140975454455198
66-67	0.0021549407532390173
68-69	0.0033222003279101513
70-71	0.002424308347393894
72-73	0.0022062488664113746
74-75	0.0025654056586178775
76-77	0.004117476082081693
78-79	0.003783973346461369
80-81	0.00397637877085771
82-83	0.002616713771790235
84-85	0.003078486790341453
86-87	0.0024371353756869837
88-89	0.6048713461889231
90-91	0.6149149093424121
92-93	0.6112592062788816
94-95	0.5972777454394143
96-97	0.595815464214002
98-99	0.5929935179895224
100	0.5946866857242101
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3898019.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.3409142519431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9416922924822	48.86157911938046
2	14.506733168362517	18.66752949722863
3	4.614284550915547	8.90661859873569
4	1.9955839298520461	5.135907780526646
5	0.9624170114433841	3.0961395203945066
6	0.5464183564505037	2.1094233970841727
7	0.34329988975107045	1.5461760138422602
8	0.22506563778279354	1.1584743121313283
9	0.1619375488615446	0.937728895092344
>10	0.6614052798007549	7.17958697033187
>50	0.031320743503495646	1.3590528768667927
>100	0.009761696007579571	1.0059537094454774
>500	7.98947865875353E-5	0.03582930893980507
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.5654056586178775E-5	0.0
8	0.0	0.0	0.0	1.7957839610325142E-4	0.0
9	0.0	0.0	0.0	3.3350273562032405E-4	0.0
10-11	0.0	0.0	0.0	4.2329193367194977E-4	0.0
12-13	0.0	0.0	0.0	4.361189619650392E-4	0.0
14-15	0.0	0.0	0.0	4.361189619650392E-4	0.0
16-17	0.0	0.0	0.0	4.4894599025812855E-4	0.0
18-19	0.0	0.0	0.0	5.130811317235755E-4	0.0
20-21	0.0	0.0	0.0	5.772162731890224E-4	0.0
22-23	0.0	0.0	0.0	6.670054712406481E-4	0.0
24-25	0.0	0.0	0.0	7.952757541715421E-4	0.0
26-27	0.0	1.2827028293089388E-5	0.0	0.001064643348326419	0.0
28-29	0.0	3.8481084879268164E-5	0.0	0.0018342650459117825	0.0
30-31	0.0	5.130811317235755E-5	0.0	0.006041530326045101	0.0
32-33	0.0	5.130811317235755E-5	0.0	0.015443742064879622	0.0
34-35	0.0	5.130811317235755E-5	0.0	0.029450856960933233	0.0
36-37	0.0	5.130811317235755E-5	0.0	0.052744740341183566	0.0
38-39	0.0	5.130811317235755E-5	0.0	0.09289333889855333	0.0
40-41	0.0	5.130811317235755E-5	0.0	0.14253393839280926	0.0
42-43	0.0	5.130811317235755E-5	0.0	0.1906994296333599	1.2827028293089388E-5
44-45	0.0	5.130811317235755E-5	0.0	0.24674071624586746	2.5654056586178775E-5
46-47	0.0	5.130811317235755E-5	0.0	0.315416625727068	2.5654056586178775E-5
48-49	0.0	5.130811317235755E-5	0.0	0.3934562658622238	2.5654056586178775E-5
50-51	0.0	5.130811317235755E-5	0.0	0.48111617721719674	2.5654056586178775E-5
52-53	0.0	5.130811317235755E-5	0.0	0.5667878991867408	2.5654056586178775E-5
54-55	0.0	5.130811317235755E-5	0.0	0.6583344001145197	2.5654056586178775E-5
56-57	0.0	5.130811317235755E-5	0.0	0.7678515676809169	2.5654056586178775E-5
58-59	0.0	6.413514146544694E-5	0.0	0.8912090987755574	2.5654056586178775E-5
60-61	0.0	7.696216975853633E-5	0.0	1.0241997281183082	2.5654056586178775E-5
62-63	0.0	7.696216975853633E-5	0.0	1.1608075794397101	2.5654056586178775E-5
64-65	0.0	7.696216975853633E-5	0.0	1.3053168801896553	2.5654056586178775E-5
66-67	0.0	8.978919805162572E-5	0.0	1.4652314419196006	2.5654056586178775E-5
68-69	0.0	1.026162263447151E-4	0.0	1.648824697878589	2.5654056586178775E-5
70-71	0.0	1.026162263447151E-4	0.0	1.8508247394381607	2.5654056586178775E-5
72-73	0.0	1.026162263447151E-4	0.0	2.057288586843727	2.5654056586178775E-5
74-75	0.0	1.026162263447151E-4	1.2827028293089388E-5	2.2670489805206184	2.5654056586178775E-5
76-77	0.0	1.026162263447151E-4	2.5654056586178775E-5	2.4942284786195246	2.5654056586178775E-5
78-79	0.0	1.026162263447151E-4	2.5654056586178775E-5	2.7538475312716537	2.5654056586178775E-5
80-81	0.0	1.026162263447151E-4	2.5654056586178775E-5	3.03745312683186	2.5654056586178775E-5
82-83	0.0	1.026162263447151E-4	2.5654056586178775E-5	3.332218237007054	2.5654056586178775E-5
84-85	0.0	1.2827028293089387E-4	2.5654056586178775E-5	3.626175244399784	2.5654056586178775E-5
86-87	0.0	1.2827028293089387E-4	2.5654056586178775E-5	3.933908480179291	2.5654056586178775E-5
88	0.0	1.2827028293089387E-4	2.5654056586178775E-5	4.1855619482614115	2.5654056586178775E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4850	0.0	30.49777	1
TCAACGC	4570	0.0	30.324348	4
GTACATG	10915	0.0	29.303953	1
ATCAACG	4795	0.0	29.104452	3
GGTATCA	3370	0.0	28.93481	1
TACATGG	10835	0.0	28.504414	2
CAACGCA	4905	0.0	28.061361	5
ACATGGG	10950	0.0	27.206127	3
AACGCAG	5245	0.0	26.411957	6
TATCAAC	6190	0.0	23.23841	2
ACGCAGA	5940	0.0	22.926386	7
CGCAGAG	6110	0.0	22.13507	8
CATGGGG	6635	0.0	21.594572	4
TACACCG	455	0.0	20.648985	5
GAGTACA	7595	0.0	19.78532	1
AGTACAT	7760	0.0	17.870468	2
TATACCG	240	3.4390105E-6	17.616167	5
CATGGGC	3725	0.0	17.52969	4
GTCTTAC	1520	0.0	16.73518	1
GTATAGA	1800	0.0	16.225548	1
>>END_MODULE