FastQCFastQC Report
Wed 25 May 2016
SRR1033137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3898019
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63240.16223625385099458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51840.13299062934275077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51750.047.2889751
GTATCAA96450.042.2554321
TCAACGC110650.036.0753484
ATCAACG110650.035.905383
CAACGCA113200.035.1800775
AACGCAG117700.033.859356
TATCAAC120700.033.0719832
ACGCAGA132800.029.8324367
GTACATG106400.029.7231311
CGCAGAG134000.029.3533448
TACATGG105700.029.1802272
ACATGGG105250.028.679213
GCAGAGT157050.024.7157339
GAGTACT100550.021.56799912-13
CATGGGG62550.021.497754
AGAGTAC137350.020.87048510-11
CAGAGTA153600.019.99335710-11
CCTATAC14100.019.3402963
AGTATCG2451.958233E-719.190835
GAGTACA74350.018.9259241
Produced by FastQC (version 0.11.5)