FastQCFastQC Report
Wed 25 May 2016
SRR1033136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267680
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC38500.033.1900864
ATCAACG39600.032.2725873
GTATCAA42900.031.743341
CAACGCA41300.030.9413345
GGTATCA27350.030.1503811
AACGCAG43050.029.6712936
GTACATG88800.028.6545011
TACATGG91000.026.3460222
TATCAAC52100.025.264262
ACATGGG92600.025.0147423
ACGCAGA51650.024.3671727
CGCAGAG52800.023.6585668
GAGTACA65550.020.0559671
CATGGGG61300.019.6957664
TCGCGTA1200.00509023719.5672369
GTACCGT2201.501694E-619.2114686
AGTACAT65350.018.3434432
GCAGAGT67900.018.2589119
CTGTCGC8300.017.5397859
GTATAGA14400.017.343051
Produced by FastQC (version 0.11.5)